• 제목/요약/키워드: oxidation content

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솔잎 추출물의 기능적 특성과 대두유 산화억제 효과에 관한 연구 (Functional Properties of Pine Needle Extract and Its Antioxidant Effect on Soybean Oil)

  • 정갑섭
    • 한국환경과학회지
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    • 제17권10호
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    • pp.1139-1146
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    • 2008
  • To investigate the functional properties and the antioxidant effect of pine needle(Pinus densiflora), the pine needle extract was obtained with methanol and its functionality was measured by spectrophotometric method, and the antioxidant experiment on soybean oil was carried out by the active oxygen method. The extraction yield of pine needle with 99% methanol was about 19%, the total flavonoid content of the pine needle-methanol extract was 11.32 mg/g on dry basis and the superoxide dismutase-like activity was 94.3%. The nitrite scavenging ability of the extract was pH dependent with the values of 77.44% at pH 1.2, 48.45% at pH 3.0 and 11.04% at pH 6.0, respectively. The peroxide value was 92.6 meq/kg at 5% dosage, 138.4 meq/kg at 2% dosage of the extract on 8 oxidation days. The period of the peroxide value to be 100 mg/kg was 4.9, 6.3 and 8.5 days at control, 2% and 5% dosage of extract, respectively. And the relative antioxidant effectiveness of the extract was 27.9% and 72.3% increase at 2% and 5% dosage, respectively, compared to control. The thiobarbituric acid value showed few differences within 4 oxidation days, but with the dosage of the extract it fairly decreased with considerable antioxidant effect to control above 4 days.

A Study on Anti-oxidative Activity of the Lithospermum Erythrorhizon Extracts for Application as a Cosmetic Ingredient

  • Park, Hee-Jeong;Lee, Ki-Young
    • 한국자원식물학회지
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    • 제26권3호
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    • pp.403-409
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    • 2013
  • This study has assessed the anti-oxidative activities and cytotoxic effects of Lithospermum erythrorhizon ethanol extract and measured the effects of tyrosinase inhibition activities with a goal of estimating the usage of the medicinal plant as an ingredient of cosmetics. First, to perform a basic test on the extract, pH and UV-spectrum were measured. According to the measurement, the extract had control functions at pH 5.5, and maximum absorbance occurred at 530nm. In particular, DPPH (1-1-diphenyl-2-picryl-hydrazyl)-inhibiting activity (IC50) and polyphenol content were 149.81 ${\mu}g/mL$ and $51.28{\pm}2.52$ mg/mL respectively. In addition, as extract concentration increased, tyrosinase inhibition activities improved as well. In raw 264.7 cell-based MTT assay, cell survival rates were 98% at 1000 ppm and 153% at 100 ppm. Therefore, it's been confirmed that there is almost no cytotoxin. According to the test results above, it appears that the Lithospermum erythrorhizon ethanol extract would be effective in anti-oxidation and application as a cosmetic ingredient.

중등 및 대학 교재 중 산화-환원반응에 대한 연계성 연구 (A Study of the Curricular Articulation of Oxidation-Reduction in the Textbooks from Middle School to College)

  • 김효겸;문성배
    • 대한화학회지
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    • 제44권2호
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    • pp.145-156
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    • 2000
  • 본 연구는 중학교 과학 교과서( 제5차 및 제6차 교육 과정), 고등학교 화학 교과서(제5차 교육과정) 및 대학 일반화학에서 화학 관련 부분 중 산화와 환원반응을 중심으로 이 학습 내용이 학생들의 사고력을 향상시키는 데 도움이 되는 적절한 연계성을 갖고 있는지를 분석하였다. 먼저 각급 학교간 배당 시간수의 차이 및 교과서 설명의 차이를 비교하였으며, 연계성 판별을 위하여 준거의 기준을 5가지로 수립하여 분석한 결과는 중학교에서 고등학교 과정의 연계시 다소 격차가 있었지만 고등학교에서 대학교 과정의 연계는 무난한 것으로 나타났다 . 이 분석결과를 이용하여 수업의 효율성을 도모하기 위하여 중등 및 대학에 이르기까지 일관된 연계성을 갖춘 각급 학교간의 새로운 교과내용 구조도를 제시하였다.

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산화촉매에 의한 대형디젤엔진의 배출가스 정화특성 -실차실험을 중심으로- (Characteristics of Exhaust Emission Reduction of Heavy Duty Diesel Engine by Oxidation Catalyst - Engine Bench Test -)

  • 조강래;김희강
    • 한국대기환경학회지
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    • 제14권5호
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    • pp.443-454
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    • 1998
  • Pt-V catalytic converter was installed on a heavy duty diesel engine and the characteristics of the emission reduction were tested using a engine dynamometer at various operating conditions. The emission reduction performance of Pt-V catalyst was also compared with that of a commercialized Pt catalyst currently being used in some of the heavy duty diesel engines in advanced countries. The effects of Pt-V and Pt catalysts on regulated and unregulated emissions were investigated using a 0.05 weight percent sulfur content fuel with an engine dynamometer Experiments for gaseous emissions (CO, HC and aldehyde) as well as particulate emissions (TPM, SOF and sulfate) have been conducted at several operating conditions such as T-7 mode, D-13 mode and S-13 mode before and after installing the Pt-V and Pt catalysts in the exhaust system. The emission reduction performance of Pt catalyst with respect to CO, HC, SOF, PAHs and aldehyde was found to be a little higher than that of Pt-V catalyst, but the Pt catalyst showed innate disadvantage of causing an increase of PM due to the sulfate formation via high SO2 conversion at high exhaust temperature, especially above 45$0^{\circ}C$.

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탄수화물과 N-3 지방산급원의 차이가 혈중중성지방함량이 높은 흰쥐의 지질대사에 미치는 영향 (Effect of Different Sources of Carbohydrate and N-3 Fatty Acid on Lipid Metabolism in Hypertriglyceridemic Rats)

  • 김우경
    • Journal of Nutrition and Health
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    • 제29권9호
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    • pp.925-933
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    • 1996
  • This study intended to compare the hypolipidemic effects among six experimental groups fed by three different dietary carbohydrates on hyperlipodemic rats. Sixty experimental animals were divided into 6 groups, SB, ST, SP and CB, CT, CP after production of hyperlipidemia fed by SB diet on Sprague-Dawley rats for 4 weeks. Rats were fed by six experimental diets for eight weeks. Hyjperlipidemic rats showed three times higher in plasma TG level not in cholesterol content compare to control group fed by stock diet. Two different dietary carbohydrates seem to be effective in body weight gain and fat cumulation as weight of epididymal fat pad. In comparison of S and C groups, C fed groups showed lowering effect in plasma TG and total lipid contents, but among S fed groups, ST and SP group showed lower than SB in this respects. Dietary carbohydrates seem to be more effective than fat in plasma lipid contents. When we compare among three different fat groups, only T groups with different carbohydrates increased in peroxisomal lipid oxidation and decreased in lipogenic enzyme activites. As same token, sucrose fed group with three fat sources seem to increase activiteies enzyme activities. In epididymal fat pad and Heart, SP and CP effect more in LPL activites than other groups. In conclusion, we can recommed to consume polysaccharides rather than disaccharide and n-3 fatty acids such as perilla and tuna oils to alleviate hypertringlycemic condition.

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Screening of the Physiological Activity of Solvent Extracts of Paulownia coreana Bark and Antioxidative Effect of the Extracts on an Edible Oil

  • Lee, Nam Gull;Jeong, Kap Seop
    • 한국환경과학회지
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    • 제22권10호
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    • pp.1317-1325
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    • 2013
  • Paulownia coreana is a medicinal, edible and industrial plant with the largest leaf, and is native to Korea. We evaluated the reducing power activities, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities (RSAs), nitrite scavenging activities (NSAs) and 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) cation RSAs by solvent extraction of P. coreana bark by using $50^{\circ}C$ hot water and $25^{\circ}C$ methanol. The antioxidative effect of P. coreana bark extract on corn seed oil was evaluated using the Rancimat test. The extraction yields on dry weight basis with 15 folds of hot water and methanol were 23.88% and 5.30%, respectively; further the flavonoid content in the hot water extract was over 2.5 times more than that in the methanol extract. The DPPH RSA of the methanol extract was substantially higher than that of the water extract, whereas the NSA of the water extract was higher than that of the methanol extract at pH 1.2. The ABTS RSAs of the two extracts were almost the same as that of ascorbic acid and 2,6-ditertiarybutyl hydroxytoluene. The two extracts of P. coreana bark in this study were found to slightly improve the oxidation stability of corn seed oil.

Effects of Packaging Methods on the Meat Quality of ${\alpha}$-Tocopherol Supplemented Broiler Chicks during Refrigerated Storage

  • Ryu, Youn-Chul;Rhee, Min-Suk;Lee, Moo-Ha;Lee, Sung-Ki;Kim, Byoung-Chul
    • Food Science and Biotechnology
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    • 제15권2호
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    • pp.248-253
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    • 2006
  • This study examined the effects of vacuum packaging and modified atmosphere packaging (MAP) on the meat quality of ${\alpha}$-tocopherol supplemented broiler chicks after prolonged refrigeration. The supplemented ${\alpha}$-tocopherol levels had no effect on the body weight and feed efficiency. The dietary treatments and packaging methods had no significant effects on the level of microbial growth, lightness, and the metmyoglobin content. The vacuum packaging showed the highest level of purge loss. The broilers supplemented with either 200 or 400 IU of ${\alpha}$-tocopherol had a lower level of malondialdehyde than the control group. The vacuum packaged meats from chicks fed the control diet showed the highest levels of lipid oxidation. The lipid oxidative stability was most improved in modified atmosphere (20% $CO_2$ + 80% $N_2$) packaged breast meat from the broilers supplemented with either 200 or 400 IU of ${\alpha}$-tocopheryl acetate.

Starch를 원료로 알데하이드 탄닝제의 제조 공정 연구 (Process for the Production of Aldehyde Tanning Agent from Starch)

  • 박재형;백인규;윤종국;조도광;정운길
    • 청정기술
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    • 제11권1호
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    • pp.51-56
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    • 2005
  • 본 연구에서는 피혁 제조 공정별 발생 폐수중 탄닝 공정에 사용되고 있는 크롬을 대체하기 위해서 전분 가수분해물인 starch을 원료로 알데하이드 함량이 다른 여러 가지 dexrin dialdehyde (DAS)를 제조하였다. 수용액상에서 산화제를 변량 하면서 (glucose에 대한 산화제의 첨가율은 1:0.3에서 1:1), 반응 조건을 달리하여 다양한 실험을 진행하였고, DAS 자체의 물성을 파악하고 가죽과의 반응 특성을 확인하였다. 제조한 DAS는 가죽의 아미노기와 결합하여 일반적인 알데하이드 탄닝제 (포름알데하이드, 그루타알데하이드)와 유사한 탄닝 특성을 보였다.

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매생이 추출물의 생리 활성과 항산화 활성에 관한 연구 (A Study on Physiological Activity and Antioxidative Activity of Maesangi(Capsosiphon fulvescens) Extract)

  • 정갑섭;이남걸
    • 한국환경과학회지
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    • 제19권4호
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    • pp.407-414
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    • 2010
  • Physiological activity and antioxidative activity of Maesangi(Capsosiphon fulvescens) extracts with distilled water or 95% ethanol were investigated. For the evaluation of physiological and antioxidative activities, some evaluation assay methods such as measurement of Hunter color value, chlorophyll a/b value, total phenolics, reducing power and thiobarbituric acid(TBA) value of soybean oil were used. Proximate composition and mineral contents of Masaengi were orders of crude protein>crude fiber>moisture>crude ash>crude lipid, and K>Ca>Mg>Na>P>Fe>Zn, respectively. In ethanol extract, the content of total phenolic compounds in Maesangi was determined to half times of that in Dasima(Laminaria). The reducing power of Maesangi-ehtanol extract was about 5 % of vitamin C and was lower than that of Dasima-ethanol extract. The TBA value of Maesangi-ethanol extract on soybean oil oxidation was about 47 % and 68.4 % to control in three and eight days oxidation, respectively. But TBA value difference was not observed significantly with the dosage below 5mL of ethanol extract.

Protective Role of Thioredoxin Peroxidase Against Ionizing Radiation

  • Lee, Su-Min;Kim, Sun-Yee;Park, Jeen-Woo
    • BMB Reports
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    • 제31권6호
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    • pp.572-577
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    • 1998
  • A soluble protein from Saccharomyces cerevisiae provides protection against a thiol-containing oxidation system but not against an oxidation system without thiol. This 25-kDa protein acts as a peroxidase but requires the NADPH-dependent thioredoxin system or a thiol-containing intermediate, and was thus named thioredoxin peroxidase. The protective role of thioredoxin peroxidase against ionizing radiation, which generates reactive oxygen species harmful tocellular function, was investigated in wild-type and mutant yeast strains in which the tsa gene encoding thioredoxin peroxidase was disrupted by homologous recombination. Upon exposure to ionizing radiation, there was a distinct difference between these two strains in regard to viability and the level of protein carbonyl content, which is the indicative marker of oxidative damage to protein. Activities of other antioxidant enzymes, such as catalase, superoxide dismutase, glucose-6-phosphate dehydrogenase, and glutathione reductase were increased at 200-600 Gy of irradiation in wild-type cells. However, the activities of antioxidant enzymes were not significantly changed by ionizing radiation in thioredoxin peroxidase-deficient mutant cells. These results suggest that thioredoxin peroxidase acts as an antioxidant enzyme in cellular defense against ionizing radiation through the removal of reactive oxygen species as well as in the protection of antioxidant enzymes.

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