• Title/Summary/Keyword: ovule culture

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Genetic Analysis on the Bacterial Blight Resistance of Suweon497, a Rice Breeding Line Developed through Wide Hybridization (벼 종간교잡 후대계통 '수원497호'의 흰잎마름병 저항성에 대한 유전분석)

  • Jeung, Ji Ung;Roh, Tae Hwan;Kang, Kyung Ho;Jeong, Jong Min;Kim, Myeong Ki;Kim, Yeon Gyu
    • Korean Journal of Breeding Science
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    • v.43 no.1
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    • pp.81-91
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    • 2011
  • Rice wild relatives have been recognized as reservoirs of genetic reinforcements to improve cultivating rice against biotic and abiotic stresses. A wild relative, Oryza. minuta(BBCC; Acc. 101141), was hybridized with a Korean Japonica cultivar, 'Hwaseong'(AA), followed by ovule culture and several times of back crossings to overcome high level of sterility. During evaluation of the introgression lines, breeding line exhibited resistance to bacterial blight with reasonable agronomic performances, and nominated as an elite breeding line, the 'Suweon497'. A mapping population, to dissect genetic basis of the resistance, was constructed by using $F_2$ progenies of the 'Suweon497' ${\times}$ 'Milyang23'. Association analysis between SSR marker genotypes and pathogenisity levels of each $F_2$ progeny revealed the end terminal region of rice chromosome 11 as the nesting place for the wild rice derived bacterial blight resistance gene, where at least four other genes, Xa3, Xa4, Xa26 and Xa31, have been reported.