• 한국가금학회지
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• 제49권4호
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• pp.221-228
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• 2022

주요 계란 난백 단백질과 그 가수분해물은 일부 금속 킬레이트 특성을 갖는 기능성 식품 성분으로 알려져 있다. 식이 oxalate를 제거하기 위해 계란 난백 단백질과 그 가수분해물을 이용할 경우 신장 결석을 예방하는데 도움이 될 것이다. 본 연구의 목적은 계란 난백 단백질인 ovalbumin, ovomucin, ovotransferrin과 이 단백질들의 효소처리를 통해 얻은 가수분해물의 biogenic oxalate chelating 활성을 측정하는 것이었다. 채소 중에서 시금치를 과일 중에서 스타푸르트(starfruit)를 선택하여 HCl처리를 거쳐 옥살레이트 추출물(oxalates extract)을 제조하였다. 계란의 난백 단백질 중, ovalbumin, ovomucin, ovotransferrin를 선택하여 이들의 가수분해물과 함께 옥살레이트 추출물과 별도로 혼합하여 3℃에서 24시간 반응을 위한 배양을 시켰다. 원심분리 후 상등액을 HPLC로 분석하여 단백질과 가수분해물의 biogenic oxalate chelating 활성을 비교 분석하였다. 사용된 모든 계란 난백 단백질과 그 가수분해물은 시금치의 oxalates에 대한 biogenic oxalate chelating 활성을 보였다. 그러나열대과일인 starfruit의 oxalates에 대한 킬레이트 활성에서는ovalbumin, hydrolyzed ovalbumin, ovomucin만이 활성을 보였다. 전반적으로, hydrolyzed ovalbumin은 시금치와 스타프루트 모두에서 oxalates chelating 효과가 가장 좋았다. 따라서 계란의 난백 단백질과 그 가수분해물의 옥살산 킬레이트 활성이 있었으며, 식이 옥살산에 대한 영양 개선을 위한 소재로 개발 가능성이 있다. 그러나, 다른 과일과 야채에 대한 옥살레이트 킬레이트 활성과 특정 생체 내 조건에서의 계란 단백질의 옥살레이트 킬레이팅 효과를 검증하기 위한 추가 연구가 필요하다.

• 한국축산식품학회지
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• 제44권4호
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• pp.885-898
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• 2024

Ovomucin (OM), which has insoluble fractions is a viscous glycoprotein, found in egg albumin. Enzymatic hydrolysates of OM have water solubility and bioactive properties. This study investigated that the immunostimulatory effects of OM hydrolysates (OMHs) obtained by using various proteolytic enzymes (Alcalase^®, bromelain, α-chymotrypsin, Neutrase^®, pancreatin, papain, Protamax^®, and trypsin) in RAW 264.7 cells. The results showed that OMH prepared with pancreatin (OMPA) produced the highest levels of nitrite oxide in RAW 264.7 cells, through upregulation of inducible nitric oxide synthase mRNA expression. The production of pro-inflammatory cytokines such as tumor necrosis factor-α and interleukin-6 were increased with the cytokines mRNA expression. The effect of OMPA on mitogen-activated protein kinase signaling pathway was increased the phosphorylation of p38, c-Jun NH2-terminal kinase, and extracellular signal-regulated kinase in a concentration-dependent manner. Therefore, OMPA could be used as a potential immune-stimulating agent in the functional food industry.

• 한국축산식품학회지
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• 제38권2호
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• pp.291-301
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• 2018

To shorten the production cycle of Zaodan, this study first pickled Zaodan by a novel technology - vacuum decompression technology. Vacuum decompression technology could reduce the pickling time of Zaodan from 20 wk to about 9 wk. The protein content, moisture and pH of the Zaodan egg white gradually decreased with a concomitant increase in salt during the pickling process. The total sulfhydryl group (SH) group content of the egg white proteins was increased to $2.43{\times}10^{-3}mol/L$ after being pickled for 30 d, whereas the content of disulphide bonds (SS) was reduced to $23.35{\times}10^{-3}mol/L$. The surface hydrophobicity was lowest after pickling for 30 d. In addition, great changes occurred in the secondary structure of the egg white proteins after pickling for 20 d. The disappearance of ovomucin was noticeable based on sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis.

• 한국가금학회:학술대회논문집
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• 한국가금학회 2006년도 제23차 정기총회 및 학술발표회
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• pp.43-58
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• 2006

As a bioreactor, bird has proved to be most efficient system for producing useful therapeutic proteins. More than half of the egg white protein content derives from the ovalbumin gene with four other proteins(lysozyme, ovomucoid, ovomucin and conalbumin) present at levels of 50 milligrams or greater. And the naturally sterile egg also contains egg white protein at high concentration allowing for a long shelf life of recombinant protein without loss in activity. In spite of these advantages, transgenic procedures for the bird have lagged far behind because of its complex process of fertilized egg and developmental differences. Recently, a system to transplant mouse testis cells from a fertile donor male to the seminiferous tubules of an infertile recipient male has been developed. Spermatogenesis is generated from transplanted cells, and recipients are capable of transmitting the donor haplotype to progeny. After transplantation, primitive donor spermatogonia migrate to the basement membrane of recipient seminiferous tubules and begin proliferating. Eventually, these cells establish stable colonies with a characteristic appearance, which expands and produces differentiating germ cells, including mature spermatozoa. Thus, the transplanted cells self-renew and produce progeny that differentiate into fully functional spermatozoa. In this study, to develop an alternative system of germline chimera production that operates via the testes rather than through developing embryos, the spermatogonial stem cell techniques were applied. This system consisted of isolation and in vitro-culture of chicken testicular cells, transfer of in vitro-maintained cells into heterologous testes, production of germline chimeras and confirmation of germline transmission for evaluating production of heterologous, functional spermatozoa.