• Title/Summary/Keyword: ovine

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The Role of Phosphatidylinositol 3-kinase and Mitogenic Activated Protein Kinase on the Differentiation of Ovine Preadipocytes

  • Choi, K.C.;Shrestha, S.G.;Roh, S.G.;Hishikawa, D.;Kuno, M.;Tsuzuki, H.;Hong, Y.H.;Sasaki, S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.16 no.8
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    • pp.1199-1204
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    • 2003
  • The aim of this study was to investigate the role of phosphatidylinositol 3-kinase (PI3 kinase) and the mitogenactivating protein (MAP) kinase pathway on the differentiation of ovine preadipocytes. In order to investigate this issue, we monitored glycerol 3-phosphate dehydrogenase (GPDH) activity during differentiation with specific inhibitors of PI3 kinase and MAP kinase-Erk kinase, LY294002 and PD098059, respectively. The preadipocytes, which were obtained from ovine subcutaneous adipose tissues, were proliferated to confluence and then differentiated to adipocytes in differentiation medium with each inhibitor for 10 days. The confluent preadipocytes and differentiated adipocytes at days 3, 7 and 10 were harvested for assay of GPDH activity. LY294002 inhibited the differentiation program in dose- and day-dependent manners during 10 days of differentiation. PD098059 did not affect GPDH activity during differentiation. Furthermore, the expression of peroxisome proliferator-activated receptor ${\gamma}2$ (PPAR-${\gamma}2$), the representative early gene of differentiation, was markedly reduced by LY294002 treatment, although PD098059 did not change it. Our results demonstrated that the activation of PI3 kinase contributes to the differentiation process of ovine preadipocytes.

Diagnostic performance of stitched and non-stitched cross-sectional cone-beam computed tomography images of a non-displaced fracture of ovine mandibular bone

  • Farzane Ostovarrad;Sadra Masali Markiyeh;Zahra Dalili Kajan
    • Imaging Science in Dentistry
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    • v.53 no.4
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    • pp.375-381
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    • 2023
  • Purpose: This study assessed the diagnostic performance of stitched and non-stitched cross-sectional cone-beam computed tomography (CBCT) images of non-displaced ovine mandibular fractures. Materials and Methods: In this ex vivo study, non-displaced fractures were artificially created in 10 ovine mandibles (20 hemi-mandibles) using a hammer. The control group comprised 8 hemi-mandibles. The non-displaced fracture lines were oblique or vertical, <0.5 mm wide, 10-20 mm long, and only in the buccal or lingual cortex. Fracture lines in the ramus and posterior mandible were created to be at the interface or borders of the 2 stitched images. CBCT images were obtained from the specimens with an 80 mm×80 mm field of view before and after fracture induction. OnDemand software (Cybermed, Seoul, Korea) was used for stitching the CBCT images. Four observers evaluated 56 (28 stitched and 28 non-stitched) images to detect fracture lines. The diagnostic performance of stitched and non-stitched images was assessed by calculating the area under the receiver operating characteristic curve (AUC). Sensitivity and specificity values were also calculated (alpha=0.05). Results: The AUC was calculated to be 0.862 and 0.825 for the stitched and non-stitched images, respectively (P=0.747). The sensitivity and specificity were 90% and 75% for the non-stitched images and 85% and 87% for the stitched images, respectively. The inter-observer reliability was shown by a Fleiss kappa coefficient of 0.79, indicating good agreement. Conclusion: No significant difference was found in the diagnostic performance of stitched and non-stitched cross-sectional CBCT images of non-displaced fractures of the ovine mandible.

Immunosuppression Associated with Ovine Foetal Skin Extracts (면양태아피부추출액(緬羊胎兒皮膚抽出液)의 세포면역(細胞免疫) 기능저하(機能低下) 효과(效果)에 관(關)한 연구(硏究))

  • Jun, Moo Hyung
    • Korean Journal of Agricultural Science
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    • v.11 no.2
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    • pp.344-351
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    • 1984
  • Crude 3 M KCI extracts of ovine foetal skin (FLS) were shown to induce serum inhibition of lymphocyte blastogenesis when injected to normal sheep. When fractionated through Sephadex C-150 columns, four protein peaks were obtained, immunosuppressive activity in vitro and in vivo being associated with Peak IV. In discussion, the immunosuppressive activity of FLS is compared with that obtained with extracts of ovine squamous cell carcinoma, and possible identity of the immunosuppressors is suggested.

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Analysis of protein-protein interaction network based on transcriptome profiling of ovine granulosa cells identifies candidate genes in cyclic recruitment of ovarian follicles

  • Talebi, Reza;Ahmadi, Ahmad;Afraz, Fazlollah
    • Journal of Animal Science and Technology
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    • v.60 no.6
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    • pp.11.1-11.7
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    • 2018
  • After pubertal, cohort of small antral follicles enters to gonadotrophin-sensitive development, called recruited follicles. This study was aimed to identify candidate genes in follicular cyclic recruitment via analysis of protein-protein interaction (PPI) network. Differentially expressed genes (DEGs) in ovine granulosa cells of small antral follicles between follicular and luteal phases were accumulated among gene/protein symbols of the Ensembl annotation. Following directed graphs, PTPN6 and FYN have the highest indegree and outdegree, respectively. Since, these hubs being up-regulated in ovine granulosa cells of small antral follicles during the follicular phase, it represents an accumulation of blood immune cells in follicular phase in comparison with luteal phase. By contrast, the up-regulated hubs in the luteal phase including CDK1, INSRR and TOP2A which stimulated DNA replication and proliferation of granulosa cells, they known as candidate genes of the cyclic recruitment.

Development of dot blot hybridization method using non-radio labeled probes for the diagnosis of malignant catarrhal fever (Dot blot hybridization에 의한 malignant catarrhal fever virus의 진단법 개발)

  • Kim, Ok-Jin
    • Korean Journal of Veterinary Pathology
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    • v.7 no.1
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    • pp.1-4
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    • 2003
  • Malignant catarrhal fever (MCF) is a systemic disease of ruminants caused by a gamma herpesvirus, ovine herpesvirus 2 (OvHV-2). Dot blot hybridization (DBH) protocols for detecting and differentiating this MCF virus were developed. OvHV-2 specific primer pairs, 556/555, were used for the amplification of target DNA. Then, the amplified DNA was labeled with incorporation of digoxigenin (DIG). The Dig-labeled probe was able to detect and differentiate specifically OvHV-2 DNA. This DBH technique can be applied to confirm the presence of MCF virus on clinical samples and to differentiate specifically between OvHV-2 infection and other viral infections.

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Mural folliculitis and alopecia caused by infection with malignant catarrhal fever virus in goat (Capra hircus) (Malignant catarrhal fever virus 감염과 관련된 goat (Capra hircus)의 mural folliculitis와 alopecia)

  • Kim, Ok-Jin;Crawford, Timothy B.
    • Korean Journal of Veterinary Pathology
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    • v.7 no.1
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    • pp.5-9
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    • 2003
  • Malignant catarrhal fever (MCF) is a systemic disease of ruminants caused by a gamma herpesvirus, ovine herpesvirus 2 (OvHV-2). Four 1-year old goats (Capra hircus), which were infected with MCF virus, OvHV-2, by being housed together with MCF virus-infected seep, were referred with a I-month history of chronic dermatitis. On the other hand, MCF virus-negative goats, which were isolated for negative control, had not those kinds of skin problems. Examination of the affected goats revealed generalized alopecia, patchy erythema, and superficial erosions with histologic evidence of mural folliculitis. Fungal culture tests and external parasite tests with the scraping skin samples were negative. However, polymerase chain reaction revealed the existence of MCF virus DNAs in the lesion. These results suggested that MCF virus may induce mural folliculitis and alopecia in goat.

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The Effect of Pronuclear Injection of Recombinant DNA on the Development Potential of Bovine Follicular Oocytes In Vitro (재조합유전자의 미세주입이 소 난포란의 체외발생에 미치는 영향)

  • 이철상;한용만;박정선;강용국;김선정;유대열;이경광
    • Korean Journal of Animal Reproduction
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    • v.17 no.3
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    • pp.193-199
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    • 1993
  • Bovine follicular oocytes were matured in two different conditions, TCM 199+10% FBS with or without hormones (0.01 unit/ml ovine follicle stimulating hormone, 0.01 unit/ml ovine luteinizing hormone and 1$\mu\textrm{g}$/ml $\beta$-estradiol). There was no significant difference in maturation and fertilization rates of the oocytes between two groups. The result indicates that hormonal treatment does not have beneficial effect on in vitro maturation and fertilization of follicular oocytes. IVF-derived cone-cell bovine embryos were injected with foreign DNA (CChcLf) by microinjection method and then co-cultrued with bovine oviductal epithelial cells. Developmental rate of microinjected embryos to blastocyst stage (21%) was similar to that of non-injected embryos(29%). This result represents that microinjected bovine embryos produced in vitro have a potential of development to normal blastocysts.

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