• Journal of Veterinary Clinics
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• v.30 no.5
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• pp.371-375
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• 2013

An 11 year-old intact female Yorkshire Terrier weighing 3.5 kg was presented with the complain of having a perineal mass, fecal incontinence and tenesmus. Physical examination revealed reducible bilateral ventral perineal mass, enlarged mammary gland and vaginal discharge. Bilateral ventral perineal hernia (BVPH) and pyometra were diagnosed based on the clinical and radiographic findings. Ovariohysterectomy was performed after general anaesthesia to treat pyometra. The BVPH was repaired by herniorrhaphy with semitendinosus muscle transposition along with colopexy and cystopexy. The postoperative radiographs and CT revealed a good correction of the BVPH. The patient showed good activity after 10 days and no complications were observed during a one year follow up period. BVPH can be successfully surgically managed by herniorrhaphy with semitendinosus muscle transposition along with cystopexy and colopexy in the dog.

• Journal of Veterinary Clinics
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• v.18 no.4
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• pp.438-441
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• 2001

Two female Yorkshire terrier was referred to Veterinary Medical Teaching Hospital, Seoul National University. In case 1, clinical signs were vomiting, anorexia and weight loss. Cystic intraabdominal mass was identified in radiographic and ultrasonographic examination. In case 2, clinical sign was abdominal distension. In radiographic and ultrasonographic examination, cellular ascites and bilateral intraabdominal masses next to kidneys were observed. Many clusters of glandular epithelial cells with anisocytosis, anisokaryosis, high N:C ratio coarse chromatin and prominent nucleoli were shown in cytologic examination of sanguineous ascites. In all two cases exploratory laparotomy was performed and enlarged ovaries were observed (Rt: 6$\times$5$\times$5 cm and Lt: 3$\times$2$\times$1 cm in case 1 and 3$\times$2$\times$1 cm bilaterally in case 2). After ovariohysterectomy histopathologic examinations were performed. Histopathologically the masses were diagnosed as ovarian adenocarcinoma and ovarian cystadenocarcinoma in case 1 and case 2, respectively. In both dogs no further chemotherapy was carried out. These two dogs had no gross evidence of tumor recurrence at the time of 3 months after tumor resection, but long-term follow-up might be needed.

• Journal of Veterinary Clinics
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• v.30 no.4
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• pp.305-309
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• 2013

Pyometra is a diestrual, chronic disease process with acute manifestations in the adult, ovary-intact bitch. Serosal inclusion cysts develop during postpartum involution as mesothelium becomes trapped during rapid uterine contraction. A 10-year-old golden retriever bitch presented with lethargy, anorexia, tachypnea, abdominal distention, and abnormal vaginal discharge. Radiographic, ultrasound, and laboratory examinations were performed. On ultrasound examination, the uterus was distended by fluid containing echogenic "snow storm" particles; cystic structures containing anechoic fluid were found adjacent to the body of the uterus. Leukocytosis, neutrophilia, and anemia were diagnosed by a complete blood cell count. The initial diagnosis was pyometra, and an ovariohysterectomy was performed. Macroscopically, the uterine body and horns were expanded and partially adhered to the abdominal wall; numerous cysts containing clear fluid protruded over the entire surface of the uterus. Escherichia coli that was sensitive to enrofloxacin, was cultured from the lumen of the uterus. Histopathological assessment confirmed a final diagnosis of pyometra and serosal inclusion cysts of the uterus.

• Journal of Embryo Transfer
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• v.18 no.2
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• pp.85-90
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• 2003

This study examined the viability of canine oocytes following storage at 4 or 38$^{\circ}C$ for 5 h. Cumulus intact oocytes were collected from domestic dog following ovariohysterectomy at local veterinary clinics. In Exp I, the oocytes that collected from ovary transport different temperatures (4 or 38$^{\circ}C$) for 5 h, were cultured for (24 or 48 h). Survivability of oocytes judged by morphological appearance and PI (propidium iodide) staining. The survival rates at 4$^{\circ}C$ ovary transport group showed significantly lower than control group (0%; 0/129 vs. 72.9%; 129/177) 48 h after culture (P<0.05). In Exp II, to assess nuclear maturation of control group oocytes (ovary transported at 38$^{\circ}C$) after in vitro cultured for 24, 48 or 96 h. After 24 h and 48 h of culture, the metaphase I to metaphase II stages (MI to MII) was 8.3% (6/72) and 8.9% (9/101), and which was not increased at 96h (9.5%; 8/84). These results show that canine oocytes remarkably sensitive to low temperature and the percentage of oocytes reaching MI to MII did not increase 96 h after culture.

• Journal of Veterinary Clinics
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• v.26 no.6
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• pp.619-621
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• 2009

Leukemoid reaction has been rarely encountered in dogs. This report describes a case of leukemoid reaction showing extremely elevated leukocyte count. A 10-year-old female Maltese dog was presented with purulent and bloody vaginal discharge. The CBC revealed severe leukemoid reaction ($976\times10^9$ cells/l, reference range 6 to $17\times10^9$ cells/ l) and mild non-regenerative anemia. On the blood smear, elevated leukocytes consist of numerous neutrophils and monocytes only. Radiographic and ultrasonographic examinations revealed generalized enlargement of the uterus, which was filled with a complex fluid. After ovariohysterectomy, Escherichia coli was isolated from the uterine fluid. To author's knowledge, it is the new record of extreme leukemoid reaction caused by local bacterial infection in dogs.

• Korean Journal of Veterinary Research
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• v.58 no.1
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• pp.33-37
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• 2018

Various trials have been conducted to develop therapies for serious untreatable diseases. Among these, those using stem cells have shown great promise, and adipose-derived mesenchymal stem cells (ADMSCs) are easier to obtain than other types of stem cells. Prior to clinical trials, characterization of ADMSCs with monoclonal antibodies should be performed. However, it is difficult to use species-specific antibodies for veterinarians. This study was conducted to confirm the panel of human antibodies applicable for use in immunophenotypic characterization of canine adipose-derived stem cells and feline ADMSCs extracted from subcutaneous adipose tissue collected during ovariohysterectomy. For flow cytometric immunophenotyping, the third passages of canine ADMSC and feline ADMSC and human CD31, CD34, CD42, CD44, CD62 and CD133 antibodies were used. Of these, CD133 reacted with canine cells (3.74%) and feline cells (1.34%). CD133 is known as a marker related with more primitive stem cell phenotype than other CD series. Because this human CD133 was not a species-specific antibody, accurate percentages of immunoreactivity were not confirmed. Nevertheless, the results of this study confirmed human CD133 as a meaningful marker in canine and feline ADMSCs.

• Journal of Veterinary Clinics
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• v.26 no.6
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• pp.655-658
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• 2009

An 11-year-old, female, Maltese dog was presented with abdominal distension and intermittent slight lameness on left hindlimb. Palpable abdominal pain and purulent vaginal discharge were observed on physical examination. Severe leukopenia with toxic change, and azotemia, hyperphosphatemia, and hyponatremia were identified on blood profile. On radiographs and abdominal sonograms, remarkable soft tissue mass containing echogenic material deviating intestines craniodorsally compatible with pyometra. On stifle radiographs, 4-5 fragmented sesamoid bone (fabella) was identified on both stifle joint with medial patella luxation. Immediate ovariohysterectomy was performed and the patient was recovered well with normal blood work. No specific treatment was attempted to the left hindlimb showing minimal and intermittent lameness. No remarkable abnormal gait was reported for 3-month follow-up period. The fragmented lateral fabella is considered congenital multipartite of lateral fabella not related to the lameness.

• Journal of Veterinary Clinics
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• v.24 no.1
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• pp.38-41
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• 2007

A 12-year-old, intact female Yorkshire terrier was presented with progressive abdominal distention. On radiographic and ultrasonographic evaluation, a large mass was detected in right upper abdomen and it had several discrete anechoic follicles and large cysts. The mass was removed by complete ovariohysterectomy and the resected specimen was histopathologically examined. Based on the gross and histopathologic findings, the mass was definitely diagnosed as malignant granulosa cell tumor (GCT). Until now, the dog has been successfully managed more than a year without complications.

• Journal of Veterinary Clinics
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• v.28 no.5
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• pp.542-545
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• 2011

A 12-year-old Yorkshire Terrier bitch evaluated with vaginal prolapsed and vaginal bleeding. Abdominal sonography and radiography demonstrated abnormal enlargement of uterus in abdominal cavity. The dog had been submitted to the vaginal mass resection and the ovariohysterectomy. In gross examination, the vaginal mass was firm and multiple cysts were detected in both ovaries and uterine horns. In microscopic examination, vaginal fibroma, uteroovarian cystadenocarcinoma were revealed. To our knowledge, this report is a very rare case of multiple complex neoplasms in the genital organs of dog. These findings may contribute to study and enhance the knowledge on genital tumors.

• Journal of Veterinary Clinics
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• v.31 no.6
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• pp.539-543
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• 2014

Vaginal tumors are the second most common types of tumor in canine female reproductive tract, and most of these tumor are diagnosed as leiomyoma. These tumors are categorized by intraluminal or extraluminal form, and tend to develop as hormone dependent manner. In this report, we describe two cases of extra-luminal form of vaginal tumor. Both tumors were located in abdominal cavity, and adhered to entire surrounding internal organs, including urinary bladder, rectum, and particularly a part of ureter. Surgical excision was performed via laparotomy without damage to the surrounding internal organs and ovariohysterectomy, as well. A diagnosis of vaginal leiomyoma was established based on histopathology.