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β-Carotene, Cucumisin Content and Fruit Morphology of Melon (Cucumis melo) Germplasm Collection

  • Hyungjun Noh;Ae-Jin Hwang;Jae-Jong Noh;Bum-Soo Hahn;On-Sook Hur;Na-Young Ro;Jae-Eun Lee;Bit-Sam Kim;Ju-hee Rhee;Jung Yoon Lee;Ji Hyun Kim;Awraris Debie Assefa;Tania Afroz;Myung-kon Kim
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2021.04a
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    • pp.48-48
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    • 2021
  • This study was carried out to investigating quality and nutritional traits of melon genetic resources. It could provide important baseline data in breeding for increased β-Carotene, cucumisin content thereby increasing the marketability of melon. To this end, we have examined some fruit morphological traits and β-Carotene, cucumisin content of 163 genetic resources. The morphological characters were recorded on the field and inside laboratory and nutritional contents for β-carotene, cucumisin was measured using spectrophotomertic and HPLC methods. Melon fruits have shown a diverse morphological characters. Green and white is dominant for fruit skin color and round and oval-shape is dominant for form in the entire collections. The β-carotene content varied between 0.5 and 233.6mg/kg, 0.7 and 226.5mg/kg, 0.4 and 189.0 mg/kg using UV-Vis and microplated reader instruments, HPLC respectively. The high β-carotene contents were characterized five melon fruit originated from South Africa, Uzbekistan, Albania, France. The cucumisin content varied between 0.3 and 33.5 unit/mg, 0.29 and 32.1unit/mg using UV-Vis and microplated reader instruments, respectively.

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Clinical and laboratory factors associated with the presence of dysmorphic oocytes in intracytoplasmic sperm injection cycles

  • Tae Eun Kim;Hyun Kyung Lee;Byung Chul Jee
    • Clinical and Experimental Reproductive Medicine
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    • v.50 no.4
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    • pp.270-276
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    • 2023
  • Objective: This study investigated the clinical and laboratory factors associated with the presence of dysmorphic oocytes in intracytoplasmic sperm injection (ICSI) cycles. Methods: The study involved 200 ICSI cycles, performed from 2020 to 2021, that yielded at least one mature oocyte. Clinical characteristics and ovarian stimulation methods were compared between 68 cycles with at least one dysmorphic oocyte (the dysmorphic group) and 132 cycles with normal-form oocytes only (the non-dysmorphic group). Dysmorphic oocytes were characterized by dark cytoplasm, cytoplasmic granularity, cytoplasmic vacuoles, refractile bodies in the cytoplasm, smooth endoplasmic reticulum in the cytoplasm, an oval shape, an abnormal zona pellucida, a large perivitelline space, debris in the perivitelline space, or an abnormal polar body. Results: The ages of the women, indications for in vitro fertilization, serum anti-Müllerian hormone levels, and rates of current ovarian endometrioma were similar between the dysmorphic and non-dysmorphic groups. In both groups, the three ovarian stimulation regimens, two types of pituitary suppression, and total gonadotropin dose were employed similarly. However, the dual-trigger method was used more frequently in the dysmorphic group (67.6% vs. 50%, p=0.024). The dysmorphic group contained significantly more immature oocytes and exhibited significantly lower oocyte maturity (50% vs. 66.7%, p=0.001) than the non-dysmorphic cycles. Within the dysmorphic group, significantly lower oocyte maturity was found in the cycles using a dual-trigger, but not in those with a human chorionic gonadotropin trigger. Conclusion: ICSI cycles with dysmorphic oocytes are closely associated with reduced oocyte maturity. This association was observed exclusively in dual-trigger cycles.

Injury Aspects of the Stone Leek Leafminer, Liriomyza chinensis Kato (Diptera: Agromyzidae) on Welsh Onion (파를 가해하는 파굴파리의 충태별 피해 양상 및 행동)

  • 최인후;김정화;김길하;김철우
    • Korean journal of applied entomology
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    • v.42 no.4
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    • pp.335-343
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    • 2003
  • An experiment was conducted to investigate types of injury inflicted by the stone leek leafminer, Liriomyza chinensis Kato (Diptera: Agromyzidae) on welsh onion. A feeding scar made by an adult female was a hole round in shape, with diameter of 0.08 mm and 0.48 mm in lesion, resulting in a white spot, many of which often form vertical dotted lines on a leaf. Egg spots were oval with 0.1 ${\times}$0.14 mm in size, one or several of which often form a V-shape in group. Feeding by adults began immediately after emergence and was very active from 4th to 5th day. Oviposition was done from 2nd to 6th day after emergence. In both feeding and oviposition, they were more active in the day time. Larvae after emergence crawled up the leaf at first, and then moved up and down to feed on mesophyll. When in high density, they feed on leaf from leaf tip to bottom, and let the leaf die. Area of damage per one larva was calculated as 72.1 $\textrm{mm}^2$. The aged larvae escaped from the leaf in early morning, usually between 5 and 7 am. Most pupation sites were distributed near plants,5cm in soil depth and within 10 cm away from the plant. Pupae of L. chinensis overwintered 10cm below soil surface and emerged from early May to late June the next year Adults then moved to welsh onions near over wintering sites, nursery, transplanted, and levee.

Mineralogical Characteristics of Tachylite occurring in Basic Dike, Basaltic Agglomerate Formation, Ulleung Island and Its Implications of Volcanic Activity (울릉도의 하부층 현무암질 집괴암 층내 염기성 암맥에서 산출되는 타킬라이트의 광물학적 특성과 화산학적 의미)

  • Bae, Su-Gyeong;Choo, Chang-Oh;Jang, Yun-Deuk
    • Journal of the Mineralogical Society of Korea
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    • v.25 no.2
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    • pp.63-76
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    • 2012
  • Tachylite, black basaltic glass formed by the rapid cooling of molten basalt, locally occurs at the Basaltic Agglomerate Formation (BAF), the lowest formation of Ulleung Island. The purposes of this study are to characterize the occurrence and mineralogy of tachylite and to elucidate its formation condition, with emphasis on its fracture pattern, which can serve as implications for the early volcanic activity of Ulleung Island. To this end, we investigated the occurrence pattern of tachylite in the field and carried out mineralogical analyses using optical microscope, XRD, EPMA, and SEM. Tachylite occurs at the chilled margin of basic dikes which are distributed in Naesujeon, Dodong and Jeodong seasides, Turtle Rock, and Yaerimwon, whose widths vary from several cm to 10 cm. It is evident that the outer surface of tachylite is dense and smooth, whereas the inner surface, if fractured, is characterized by conchoidal fracture. The matrix of tachylite consists of amorphous, glass and some fine-grained phenocrysts present in tachylite include biotite, anorthoclase, sanidine, plagioclase, hornblende, and Fe-Ti oxides. The fracture patterns characteristic of tachylite are subrounded, oval, or less commonly polygonal, bounded by joints to form globule or lump. Taking into account texture and mineralogy, tachylite is interpreted to have undergone little subsequent alteration at low temperature via hydration or hydrolysis that could form clay minerals after it was formed. Because tachylite with peculiar fractures occurs as dikes in a close association with BAF, its presence is considered as reliable evidence that when tachylite formed, the most part of BAF was still under subaqueous conditions, or at least saturated with seawater.

Studies on the Artificial Seedling Production of Geoduck Clam, Panope japonica II. Development of Egg and larvae (코끼리조개의 인공종묘생산에 관한 연구 II. 난발생과 유생의 발달)

  • Lee, Chae-Sung;Rho, Sum
    • Journal of Aquaculture
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    • v.10 no.1
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    • pp.25-32
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    • 1997
  • Develoment precess and characteristics of eggs of the geoduck clam, Panope japonica are reporting in this study. Eggs and sperm were excised from gonad, artificially fertilized in an aquarium, reared under various temperature regimes, and record and record the larval period and the time need to reach a certain larval stage from ferilization. Unfertilized eggs of P. japonica appeared to be oval with a mean diameter of $70\mu$m and they became spherical after fertilization. The eggs of P. japonica can be classified as demersal. At a constant water temperature of $ 11^{citc}C$, it took 4 hours form fertilization to become four-cell stage, two days to become trochophore larvae, three days to become D-shape larvae, twenty-three days to become umbo stage, and thirty-six days to become fully grown veliger ready form settlement. A negative correlation was observed between the water temperature and the larval period of P. japonica. From fertilization to D-shape larvae, it took five days at 8$^{\circ}C$, while it was only two days to become D-shape larvae at $ 17^{citc}C$. Time required to D-shape larvae from fertilization was proportional to temperature, and the relationships were expressed as follows : To 8-cell stage, 1/t=0.0209 w-0.1167 (r=0.9967) To blastula stage, 1/t=0, 0055 w-0.0192 (r=0.9825) To trochophore stage, 1/t=0.0034 w-0.0155 (r=0.9907) To D-shape larvae stage, 1/t=0.0014 w-0.0023 (r=0.9843) (t, time in hours ; w, water temperature) Bioligical minimum temperature for egg development was calculated as 3.82$^{\circ}C$ in average.

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Determination of the Period of the Formation and Size of Sieve Element Area and Sieve Pore (Streptanthus tortus 조직배양 세포에서 사공의 형성시기와 사공 영역과 사공의 크기 결정)

  • Cho, Bong-Heuy
    • Journal of Plant Biotechnology
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    • v.29 no.1
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    • pp.41-44
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    • 2002
  • During the phloem development from parenchyma cells in a suspension culture of Streptanthus induced sucrose carrier and glucose carrier disappeared. Sieve element area and sieve pore induced suspension culture of Streptanthus were formed almost at the last period of the synthesis of sieve endoplasmic reticulum (SER) and p-protein. The new synthesized cell wall begann to digeste only after the new cell wall was surrounded by SER. The digested region of the cell wall and the formed region of sieve pore were regular comparatively. The completed sieve pore was an oval form, and the outer portion of sieve pore varied, ca 1.2 ${\mu}{\textrm}{m}$~1.6 ${\mu}{\textrm}{m}$ in longitudinal, 0.8 ${\mu}{\textrm}{m}$~1.3 ${\mu}{\textrm}{m}$ in tangential, and the inner size of sieve pore was irregular form of a star-like shape. The number of sieve pore between sieve cells was ca 2~7 per ${\mu}{\textrm}{m}$$^2$ and the sieve pore wall with callose was 0.05 ${\mu}{\textrm}{m}$~0.07 ${\mu}{\textrm}{m}$ in thickness. The energy for the formation of sieve element area and sieve pore might be supplied by mitochondria near the new cell wall and the role of SER remains to be illucidated.

Development of the Trichomes in Floating Leaves of Salvinia Species (생이가래속(Salvinia) 부유엽 모용의 분화발달)

  • Seo, Ae-Ri;Kim, In-Sun
    • Applied Microscopy
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    • v.38 no.2
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    • pp.117-124
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    • 2008
  • Salvinia is an aquatic plant forming dimorphic leaves that have been modified into floating and submerged leaves. A air of floating leaves plays an important role for the floating and photosynthesis while the submerged leaves, which are lim and long, have the form and function of root. Many aquatic plants develop trichomes in the epidermis but in Salvinia, richomes grow densely in the epidermis of the dimorphic leaves. The present study examined the differentiation pattern of trichomes developing in the floating leaves of S. natans and S. molesta by scanning and transmission electron microscopy. Trichomes developing in the floating leaves of Salvinia showed very different patterns. In S. natans, they were arranged in a V-shape form, having 20${\sim}$25 rows at $18{\sim}25^{\circ}$ on both sides of the lamina divided by the midrib in the floating leaf. In each row, 8${\sim}$10 oval-shaped cells, $200{\sim}290{\mu}m$ in length, were arranged in a spiral fashion. Four trichomes of this form made a trichome unit, but their apical parts were separated from one another and developed into the so-called 'knuckle-crane' type. On the other hand, in S. molesta, trichomes differentiated in a unique pattern quite different from those of S. natans. At the early stage of differentiation, trichomes protruded from the epidermis and then 4${\sim}$6 cylindrical cells grew $400{\sim}600{\mu}m$ long and the four trichomes formed as an unit. The four grouped trichomes were interconnected through their apex and developed in the 'egg-beater' type. Then $300{\sim}600{\mu}m$ long multi-cellular stalk cells grew and protruded out of the epidermal surface from the basal part of the trichomes. Such a structural characteristic of trichomes is considered to play a very important role along with the aerenchyma tissue in the leaf mesophyll tissue for the floating of Salvinia on the water surface.

Behcet`s Syndrome with Aortic Aneurysm: A Case Report (Bechet`s 병과 합병된 상부대동맥류: 치험 1례 보고)

  • Gang, Jeong-Ho;Lee, Jeong-Ho;Yu, Hoe-Seong
    • Journal of Chest Surgery
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    • v.10 no.1
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    • pp.98-105
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    • 1977
  • A 36 year old blindman, engineer was admitted with chief complaints of hemoptysis, recurrent sore throat, pyoderma in genital organ, uveitis and thrombophlebitis for 10 years. Above the chief complaints were remission or exacerbation during hospitalization. Physicalexamination showed that left radial, ulnar & brachial pulse was not palpable. No bruit or murmur was obtained over the mass. Neurologic examination revealed no significant finding.On admission, chest P-A showed hen egg sized round & oval compact hazy density on left upper lung field. Bronchogram revealed no pathological finding and Lt. tomogram showed well define large,ovoid mass density in the superior mediastinum. Fluoroscopy finding showed nonpulsatile on left upper lung field. Pre-op. aortography was not taken, under the impression of lung Ca. rule out .sortie aneurysm, exploratory operation was performed through the 2nd intercostal space, Lt. It was performed that the mass was ascending sortie aneurysm of saccular type. Direct aneurysmectomy with multiple figure of eight suture were done without any prosthetic graft. Post-op. control I.V.C graphy showed completely obstruction sign. Postopcontrol aortography revealed good surgical result. Final, histopathological answered non-specific sortie aneurysm, saccular type. Post-op. courses were uneventful except mild neurologic disturbance with subclavian steal syndrome and associated with both lower leg pitting edema due to inferior vena cava obstruction. After op, 3 month later, discharged to home, with big systemic problem. Behcet`s syndrome reviewed with related literatures. The coexistence of mouth and genital ulceration with hypopyon mentioned by hippocrates and described by various workers in the early part of this century was first defined as a syndrome by Behcet in 1937. In 1937 Behcet described a chronic relapsing triple symptom complex of oral ulceration, genital ulceration, and ocular inflammation. The place of the syndrome as part of a systemic disorder in now clearer, and the under lying pathology appears to be a vasculitis. The disease runs a- chronic course, blindness being the greatest disability and control nervous system involvement a cause of death. Thrombophlebitis is fairly frequent, france et al [1951] giving an incidence of 25% and Dowling [1961] 12%, superficial thrombophlebitis migrans and thrombosis of large veins, including venae cavae [Thomas, 1947: Boolukos 1960] are recorded. Little attention has been paid to arterial involvement. Mishima et al. [1961] described resection cf an aortic aneurysm in a 38 year old man with Behcet`s syndorme. Mounsey in a clinicopathological conference described a case [Brit, med. J., 1966] of ruptured aortic aneurysm in Bechcet`s syndrome treated by aorto-iliac graft. Also, Shikano and Oshima et al [1963] recorded two aneyrysm of smaller arteries. Unfrequently, aortic aneurysm was presumed to be secondary to osteomyelitis of the lumber spine, though the possible association between aortic aneurysm and Behcet`s syndrome was raised. A further case is reported here, in which ascending aortic aneurysm with Behcet`s Ds. appeared to form part of this generalized disease. This is a case report of surgical experience of Behcet`s Ds. with ascending aortic aneurysm which had nearly all the typical clinical features. Above mentioned and was reviewed with related literatures.

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Ultrastructure of the Compound Eye of the Rice Brown Planthopper, Nilaparvata lugens (StaL) (Homopteera : Auchenorhyncha : Delphacidae) (벼멸구 겹눈의 미세구조)

  • Young Nam Youn
    • Korean journal of applied entomology
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    • v.34 no.3
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    • pp.266-277
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    • 1995
  • The adult brown planthopper possesses tow oval shaped compound eyes which, on their ventral borders, curve around the base of the antennae. Compound eye of the adult brown planthopper is recognised apposition eye which each ommatidium is optically isolated from it surroundings, the rhabdoms receiving light only from their own corneal lens. Each ommatidium possesses its own dioptric apparatus formed from the cuticular cornea and an underlying crystalline cone. The retinula cells lying immediately beneath the crystalline cone have their individual rhabdomeres tightly opposed to form one central, closed rhbdom. The rhabdom stretches from the spex of the crystalline cone nearly to the basement membrane and is approximately 110~120 $\mu\textrm{m}$ in length. The crystalline cone is surrounded by a pair of primary pigment cells an these in turn are surrounded by accessory pigment cells. Accessory pigment cells extend beyond the crystalline cone surrounding the retinular cells in the distal region of the eye. The crystalline cone is surrounded by the distal-most regions of the retinula cells show the presence of seven cells and sections taken proximally in the last quarter of the omatidium before the basement membrane is reached, reveal the presence of a small, eighth retinula cell which also contributes to the central rhabdom. Each ommatidium has a central rhabdom formed from the modified inner border of all of the retinula cells. Th rhabdom consists of micrvilli arising from the inner wall of each retinula cell. In cross section th microvilli exhibit a characteristic honeycomb appearance. Pigment cells comprise the primary pigment cells enveloping the crystalline cone, the accessory pigment cells extending from the inner surface of the comea to the basement membrane and the small pigment cells of the basement membrane.

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Anatomical studies on trigeminal nerve of Korean native goat (한국재래산양 삼차신경에 관한 해부학적 연구)

  • Shin, Nam-Shik;Lee, Heungshik S.;Lee, In-se;Kang, Tae-Cheon;Kim, Jin-sang;Lee, Jong-Hwan;Seo, Jehoon
    • Korean Journal of Veterinary Research
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    • v.38 no.3
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    • pp.474-487
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    • 1998
  • The present study was undertaken to investigate the morphological characteristics of trigeminal nerve in the Korean native goat by macroscopic methods. Trigeminal nerve was originated from the lateral side of pons, and extended shortly forward to form trigeminal ganglion at the opening of oval foramen. Thereafter this nerve was divided into maxillary, mandibular and ophthalmic nerve. Ophthalmic nerve gave off the zygomaticotemporal branch, frontal nerve, frontal sinus branch, and was continued as the nasociliary nerve. Maxillary nerve gave rise to the zygomaticofacial branch, accessory zygomaticofacial branch, communicating branch with oculomotor nerve, pterygopalatine nerve, caudal superior alveolar branch, malar branch and was continued as the infraorbital nerve. Mandibular nerve was divided into the masseteric nerve, buccal nerve, lateral pterygoid nerve, medial pterygoid nerve, nerve to tensor tympani m., auriculotemporal nerve, and furnished the inferior alveolar nerve and lingual nerve as terminal branches. The course and distribution of the trigeminal nerve in the Korean native goat appeared to be similar to that in other small ruminants such as sheep and goat. But the main differences from other small ruminants were as follows : 1. There was no accessory branch of the major palatine nerve. 2. The caudal superior alveolar branch was directly branched from the maxillary nerve. 3. The communicating branch with oculomotor nerve was originated from maxillary nerve or common trunk with zygomaticofacial branch. 4. The malar branch arose from the maxillary nerve at the rostral to the origin of the caudal superior alveolar branch. 5. The inferior alveolar nerve originated in a common trunk with the lingual nerve. 6. The mylohyoid nerve arose at the origin of the inferior alveolar nerve. 7. The zygomaticotemporal branch was single fascicle, and gave off lacrimal nerve and cornual branch. 8. The base of horn was provided by the cornual branches of zygomaticotemporal branch and infratrochlear nerve of nasociliary nerve.

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