• 한국식품영양학회지
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• 제27권4호
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• pp.684-691
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• 2014

This study was carried out to investigate the antioxidant activity and changes in major functional components of fermented Gastrodia elata Blume. Fermented G. elata Blume powder by Phellinus linteus repeated thrice ($3^{rd}$ FGP) showed more DPPH radical scavenging activity than a non-fermented G. elata Blume powder (NFGP), and once fermented G. elata Blume powder ($1^{st}$ FGP) at a concentration of 500 and 1,000 ppm. Free radical scavenging activity of $3^{rd}$ FGP was similar to that of BHA at a dose of 1,000 ppm. Moreover, the ABTS radical scavenging activity of the $3^{rd}$ FGP increased compared to NFGP and $1^{st}$ FGP at a concentration of 31.25 ppm. Total polyphenols and flavonoid contents were increased as fermentation progressed. Ergothioneine content was increased more than 8 times in the $1^{st}$ FGP, 3 times in the $3^{rd}$ FGP, respectively than NFGP. In conclusion, this study indicated that the antioxidant activity and functional component contents of G. elata Blume were increased depending on the fermentation number.

• International Journal of Concrete Structures and Materials
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• 제8권2호
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• pp.173-182
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• 2014

An experimental investigation was carried out to evaluate the mechanical properties of concrete mixtures in which coarse aggregate was partially (30, 50 or 70 %) replaced with pre-washed municipal solid waste incineration (MSWI) bottom ash. Results indicated that bottom ash reduced the compressive strength, elastic modulus, and levels of heavy metals in leachate when used as a replacement for gravel, and that the maximum amount of MSWI bottom ash in concrete should not exceed 50 %. To analyze the effect mechanism of bottom ash in concrete, the degree of hydration and the following pozzolanic reaction characterized by the pozzolanic activity index, and the porosity distribution in cement mortar. The study indicates that improved properties of concrete are not solely later strength gain and reduced levels of heavy metals in leachate but also the progression of pozzolanic reactions, where a dense structure contains a higher proportion of fine pores that are related to durability.

• 생약학회지
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• 제31권1호
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• pp.72-76
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• 2000

This study was carried out to evaluate cytotoxic effects of Ajuga multiflora Bunge extracts on murine leukemia tumor $(P388D_1)$ cell lines. Disruptions in cell organelles were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazoliumbromide (MTT) assay. The comparison of $IC_{50}$ values of Ajuga multiflora Bunge extracts in L1210 and $P388D_1$ cell lines showed that their susceptibility to these extracts decreased in the following order: Adriamycin>methanol extract>chloroform extract>ethyl acetate extract>hexane extract>water extract by the MTT assay. In order to develop an antimicrobial agent, dried Ajuga multiflora Bunge was extracted with several solvents, and then antimicrobial activity was investigated. The minimal inhibitory concentration (MIC) of the extract against microorganisms were also examined. Antimicrobial activities of amocla and ketoconazole as references were compared to those of extracts of $H_2O$, n-hexane, chloroform, ethyl acetate and methanol. The antimicrobial activity of all extracts from the sample had growth inhibition activity against gram-negative bacteria, gram-positive bacteria and fungi $(MIC>200\;{\mu}g/ml)$. These results suggest that the methanol soluble extract of Ajuga multiflora Bunge may be a valuable choice for the studies on the treatment of murine leukemia tumor cell lines.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 2001년도 춘계학술대회
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• pp.103-112
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• 2001

Bone homeostasis is maintained by a balance between activities of osteoblasts(bone forming cells) and osteoclasts (bone resorbing cells). The activities of these cells are closely regulated by multiple factors including hormones and cytokines. The cessation of estrogen at menopause disrupts the balanced regulation and is the main cause of osteoporosis in postmenopausal women. Recent molecular biological studies led to a discovery of tumor necrosis factor(TNF) and TNF receptor families genes that play critical roles in the regulation of osteoclast formation and function. RANKL (receptor activator of nuclear factor kappa B ligand; also called ODF, TRANCE, and OPGL) expressed on cells supporting osteoclast is essential for osteoclast differentiation, activation, and survival. RANK, the counter-receptor for RANKL, is expressed on progenitor and mature osteoclasts. The interaction between RANKL and RANK is requlated by a soluble decoy receptor OPG (osteoprotegerin). Gene knock out studies of these molecules showed profound effects on bone. These results prompted development of new strategies for treatment of bone diseases. Inhibition of osteoclast activity by blocking the RANKL-RANK interaction using OPG is being attempted. Research on the signaling pathways of RANK is also actively carried out. Screening natural products that inhibit the RANKL-RANK interaction or the activity of obteoclasts would be another effective means to a new drug target for bone resorbing diseases.

• 한국초등과학교육학회지:초등과학교육
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• 제35권2호
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• pp.229-242
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• 2016

The purpose of this study was to analyze the elementary teachers' conceptions on the inquiry activity about 'make a fossil model' focusing the 6th, 7th, 2007 revised, and 2009 revised elementary science textbook. This study is intended to find causes of teachers' conceptions and to derive educational implications. We have analyzed questionnaires which were collected from 77 elementary school teachers. It was carried out about comparisons actual fossil with fossil model in each step of the inquiry activity, the lesson goal, the thought for changes of the inquiry activity, the improvement of the inquiry and conception related fossil. Teachers have a general understanding about the contents of the inquiry activity but some of them have partially incorrect understanding. And they emphasize the understanding of the concept on lesson goal. It is necessary to improve inquiry activity reflecting teachers' thinking about the merit and weaknesses. There was not statistically difference in understanding of fossil related concepts according to teachers' career, teaching or training experience. Through this research, there is a need to improve a textbook, a guide book, a teachers' training. So, teachers who have professionalism about science textbook be able to teach students.

• 한국식품과학회지
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• 제48권5호
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• pp.515-520
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• 2016

본 연구에서는 꽃송이버섯 자실체와 기부의 항산화 활성, 베타글루칸 함량 및 면역 활성을 비교하였다. DPPH 라디컬제거능 실험결과 꽃송이버섯 기부의 항산화 활성이 자실체에 비해 약 2.3배 높은 활성을 보였다. 건조 중량 기준 꽃송이버섯 추출물의 베타글루칸 함량 또한 기부가 23.7 g/100 g로 자실체의 함량 13.8 g/100 g보다 유의하게 높은 것으로 측정되었다. 면역 저하에 대한 꽃송이버섯 추출물의 투여는 $TNF-{\alpha}$, IL-2, IL-10, IgE 함량에서 유의미한 결과를 도출하였지만, 자실체와 기부의 효과는 유의적인 차이가 없었다. 혈액 내의 백혈구 함량 및 비장의 무게는 꽃송이버섯 자실체보다 꽃송이버섯 기부 추출물을 경구 투여한 실험군에서 효과적이었다.

• 한국식품과학회지
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• 제45권5호
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• pp.550-556
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• 2013

오미자 유효성분의 식품 소재화를 위한 기초 자료를 얻기 위해 물과 에탄올을 이용하여 에탄올 농도(%), 추출온도($^{\circ}C$), 추출시간(h)을 달리한 추출물의 이화학적 특성과 항산화 작용을 확인하였다. 환원당 함량은 추출온도가 증가할수록 증가하였고 에탄올 농도는 50%, 추출시간은 4시간 추출하였을 때 높은 함량을 나타내었다. 산도는 에탄올 농도 50%, 추출시간 8시간, 추출온도 $100^{\circ}C$일 때 가장 높은 적정 산도를 나타내었다. 색도는 명도($L^*$)는 추출온도, 추출시간이 증가할수록 감소하는 것을 알 수 있었고, 오미자의 주 색상인 적색도($a^*$)는 에탄올 농도, 추출온도, 추출시간이 증가할수록 감소하는 경향을 보였다. 총 폴리페놀 함량은 50% 에탄올 추출물에서 가장 높은 함량을 나타내었고 추출시간과 온도가 증가할수록 유의적으로 증가하였다. 이와 같은 경향은 DPPH 및 ABTS 라디칼 소거능에서도 유사한 경향을 나타내었다. 각 추출조건별 실험 인자들의 상관성을 분석한 결과, 에탄올 농도는 총 페놀 및 ABTS 항산화능과 매우 높은 상관성을 나타내었다. 추출시간과 온도에 따른 총 페놀함량은 DPPH 및 ABTS 항산화능, 환원당 함량, 적색도, 적정산도에서 강한 상관관계를 나타내었고, 각 실험 항목 사이에서도 모두 높은 상관성을 나타내었다. 이상의 결과로 볼 때 오미자의 최적 추출조건은 용매는 50% 에탄올, 추출시간 4-6시간, 추출온도는 $60-80^{\circ}C$의 조건이 가장 바람직하다고 사료된다.

• 대한본초학회지
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• 제22권4호
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• pp.51-58
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• 2007

Objectives : Investigation of the memory and cognitive enhancing effect of HT008-1 in scopolamine induced amnesia mice. Methods : At 60 min before acquisition trials, HT008-1 (30, 100, 300 mg/kg p.o.) was administered, and 30 min later, mice were injected with scopolamin (1.0 mg/kg, i.p.). In the passive avoidance test, acquisition trials were carried out 30 min after a single scopolamine treatment. Retention trials were carried out 24h after acquisition trials. Y-maze test was carried out 30 min after a single scopolamine treatment. Spontaneous alternation behavior during an 8-min session was recorded. Inhibitory effects of HT008-1 (0.01, 0.1, 1.0 mg/ml) on AChE activity was measured. Result : HT008-1 ameliorated scopolamine-induced learning impairments and spatial cognitive function in passive avoidance and Y-maze test, respectively. Moreover HT008-1 showed a significant inhibitory effect on AChE activity. Discussion: This study presented that eMultiherb mixture HT008-1 enhanced learning memory and spatial cognitive function in scopolamine-induced amnesia mice. These results suggest that the effect of HT008-1 may be dependent on the inhibition of AChE activity.

• BMB Reports
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• 제43권9호
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• pp.629-634
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• 2010

Endotoxin including lipopolysaccharide (LPS) confers organ tolerance against subsequent challenge by ischemia and reperfusion (I/R) insult. The mechanisms underlying this powerful adaptive defense remain to be defined. Therefore, in this study we attempted to determine whether nitric oxide (NO) and its associated enzymes, inducible NOS (iNOS) and endothelial NOS (eNOS, a constitutive NOS), are associated with LPS-induced renal tolerance against I/R injury, using iNOS (iNOS knock-out) or eNOS (eNOS knock-out) gene-deleted mice. A systemic low dose of LPS pretreatment protected kidney against I/R injury. LPS treatment increased the activity and expression of iNOS, but not eNOS, in kidney tissue. LPS pretreatment in iNOS, but not eNOS, knock-out mice did not protect kidney against I/R injury. In conclusion, the kidney tolerance to I/R injury conferred by pretreatment with LPS is mediated by increased expression and activation of iNOS.

• 한국초등과학교육학회지:초등과학교육
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• 제35권1호
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• pp.54-64
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• 2016

The purpose of this study is to develop instructional strategy which utilizes thinking maps focused on drawing as a measure to enhance science learning motivation, self-directed learning activity and science academic achievement of learners, and to examine the effects of its application. The target unit for this study is 'life cycle of plants' in the fourth grade of elementary school. Two classes of 4th grades of elementary school were selected and divided into two groups. The learners of experimental group have completed thinking map by drawing a picture to express the results to be observed and measured, and used it to arrange the learning contents. The result of this study is as follows. First, it is proven that using thinking maps focused on drawing actually helped improving the motivation of learners to study science. Second, it is proven that this strategy was effective to change their self-directed learning ability in positive ways. Third, it contributed to the improvement of learners' science academic achievement. We found out that the application of this strategy enabled them to enjoy the mapping using drawing, to be immersed in learning, to better recognize the scientific concepts and the structure of learning contents, and to have a positive awareness of the usefulness of thinking maps focused on drawing.