• Journal of Microbiology and Biotechnology
• /
• v.11 no.1
• /
• pp.72-78
• /
• 2001

It is possible ot prepare protoplasts of the zygomycete fungus, Phycomyces blakesleeanus, by digesting the cell wall of spore germlings with commercially available chitinase and chitosanase. However, the cells without any cell walls immediately form large aggregates, and thus, it is difficult to isolate the individually separated protoplasts. Inherent problem with the formation of aggregates in preparing protoplasts could be solved by the use of bovine serum albumin (BSA). As a result, we were able to prepare a large number of single protoplsts quickly and easily. We took time-lapse photomicrographs of the formation of protoplasts, and found that there were certain regions of the cell wall of spore germlings that were sensitive to chitinase and chitosanase, although the cell wall of the original spores is known to be insensitive to these enzymes. There are two kinds of cell walls on a spore germling; one with a bound wheat germ agglutinin (WGA), and the other a bound concanavalin A (ConA). Furthermore, only cells with walls which had bound WGA were able to regenerate, while those with walls with bound ConA were not able to regenerate.

• Structural Engineering and Mechanics
• /
• v.85 no.5
• /
• pp.655-664
• /
• 2023

Reinforced concrete (RC) shear wall structures are one of the most widely used structural systems to resist seismic loading all around the world. Although there have been several efforts to provide conceptually simple procedures to reasonably assess the seismic demands of structures over recent decades, it seems that lesser effort has been put on a number of structural forms such as RC shear wall structures. Therefore, this study aims to represent a simple linear response spectrum-based method which can acceptably predict the nonlinear displacements of a non-ductile RC shear wall structure subjected to an individual ground motion record. An effective period and an equivalent damping ratio are introduced as the dynamic characteristics of an equivalent linear SDOF system relevant to the main structure. By applying the fundamental mode participation factor of the original MDOF structure to the linear spectral response of the equivalent SDOF system, an acceptable estimation of the nonlinear displacement response is obtained. Subsequently, the accuracy of the proposed method is evaluated by comparison with another approximate method which is based on linear response spectrum. Results show that the proposed method has better estimations for maximum nonlinear responses and is more utilizable and applicable than the other one.

• Earthquakes and Structures
• /
• v.25 no.5
• /
• pp.385-398
• /
• 2023

The parameters of the multiple-vertical-line-element model (MVLEM) of reinforced concrete (RC) shear walls are often empirically determined, which causes large simulation errors. To improve the simulation accuracy of the MVLEM for RC shear walls, this paper proposed a novel method to determine the MVLEM parameters using the artificial neural network (ANN). First, a comprehensive database containing 193 shear wall specimens with complete parameter information was established. And the shear walls were simulated using the classic MVLEM. The average simulation errors of the lateral force and drift of the peak and ultimate points on the skeleton curves were approximately 18%. Second, the MVLEM parameters were manually optimized to minimize the simulation error and the optimal MVLEM parameters were used as the label data of the training of the ANN. Then, the trained ANN was used to generate the MVLEM parameters of the collected shear walls. The results show that the simulation error of the predicted MVLEM was reduced to less than 13% from the original 18%. Particularly, the responses generated by the predicted MVLEM are more identical to the experimental results for the testing set, which contains both flexure-control and shear-control shear wall specimens. It indicates that establishing MVLEM for RC shear walls using ANN is feasible and promising, and that the predicted MVLEM substantially improves the simulation accuracy.

• Journal of Korea Foresty Energy
• /
• v.20 no.1
• /
• pp.62-72
• /
• 2001

To elucidate the behaviors of alkali swelllng of woods. the dimensional change in cross section of cell elements of four major Korean woods during alkali swelling were examined by an optical microscory, an imaging analysis method and an X-ray diffrartion During alkali swelling, tracheid diameter of Larix kaempferi wood showed greater swelling property than that of Pinus koraiensis wood, and the cell wall swelled highly over 10% sodium hydroxide solution treatment. The radial diameter of vessel elements in earlywood shrunk, but it swelled slightly in tangential direction. When treated with 5% NaOH, the wall thickness of wood fiber increased about three times over the original one. The thickness of cell wall in all elements and the diameter of wood fiber and tracheid showed almost isotropic shrinkage. The diameter of cell elements during the mercerization process decreased, but cell wall thickness Increased. Crystal transformation of cellulose in wood was not occurred by alkali treatments. but relative crystallinity and crystallite width of the woods increased slightly. Consequently, it was demonstrated that the swelling properties of woods were dependant on wood species, cell elements and alkali concentration.

• Proceedings of the Botanical Society of Korea Conference
• /
• 1998.07a
• /
• pp.10-18
• /
• 1998

For improvement of plants in disease resistance, it is most important to elucidate the mechanism to perceive and respond to the signal molecules of invaders. A model system with pea and its pathogen, Mycosphaerella pinodes, showed that the fungal elicitor induced defense responses in all plant species tested but that the suppressor of the fungus blocked or delayed the expression of defense responses and induced accessibility only in the host plant. In the world, many researchers believe that the pathogens` signals are recognized only on the receptors in the plasma membranes. Though we found that the ATPase and polyphosphoinositide metabolism in isolated plasma membranes responded to these fungal signals, we failed to detect specific actions of the suppressor in vitro on these plasma membrane functions. Recently, we found that ATPase (NTPases) and superoxide generating system in isolated cell wall were regulated by these fungal signals even in vitro, especially, by the suppressor in a strictly species-specific manner and also that the cell wall alone prepared an original defense system. The effects of both fungal signals on the isolated cell wall functions in vitro coincide perfectly with those on defense responses in vivo. In this treatise, we discuss the key role of the cell wall, which is plant-specific and the most exterior organelle, in determining host-parasite specificity and molecular target for improvement of plants.

• Journal of Conservation Science
• /
• v.26 no.4
• /
• pp.445-458
• /
• 2010

The deterioration and structural damage such as exfoliation, cracks, and separation of painted layer on the wall paintings of Maitreya Hall in Geumsan-sa temple have been accelerated since it was re-positioned to the original place after the dismantling from the building in 1993. The examination of which result and analysis described in this study, is a preliminary survey for establishing conservation plan of the wall paintings. It aimed at the understanding of the physical and chemical characteristics of the materials applied in the 1993 conservation. The research focused on the south walls which displayed the worst condition compared to other walls. Samples for the examination for the understanding of micro-structure, chemical composition, cristalisation, and particle distribution, were collected for finishing, middle, and consolidated layers of the walls between pillars and the ones between brackets. Those samples were collected from separated fragments of the walls. The sample analysis displayed that: 1. the 1993 conservation used the similar type of weathered soil as the original for the finishing layer, and such soil and sand for the middle layer; 2. those walls are composed of a group of mineral particles which are relatively equal in size and shape and in their distribution; 3. the mineral particles were cohered forming solid aggregate due to the application of acrylic resin for the reinforcement on the wall. The main composition of crystalisation on the first and the second reinforcement layers of the back walls were lime plaster ($CaSO_4{\cdot}2H_2O$). The overall examination confirmed that the priority of the future conservation treatment should be given to the removal of the first and the second layers of reinforcement and the treatment on the back walls which were partially consolidated.

• Korean Journal of Heritage: History & Science
• /
• v.42 no.4
• /
• pp.50-73
• /
• 2009

Recently old stone walls were designated as registered cultural properties that meant an extension of categories about cultural properties from a spot area to whole area. Moreover given the changing situation of residential pattern, which is due to rapid social change, this designation can be seen as a significant measure to keep as intact as possible traditional landscapes in agricultural and fishing villages. In this paper, I analyze the symbol system and meaning of old stone walls and attempt to pick out the cultural elements which are related to them. These days we have made efforts to various aspects for which make traditional cultural resources into cultural contents. But many studies had done before emphasized aspects for beauty only. Especially existing studies about an old stone wall was mainly focused on architectural interpretation and tourist route. So we need to build a plot around oral research and need a creative approach for sharing with tourists. Cultural contents combine the original form, potential and capabilities with media by detecting original form of culture and finding out the worth and meaning. In this paper examined the probability of using by investigating a stone wall in Sang-hak Village that is related with recovering of places to live in contemporary society and finding cultural contents. I suggest more creative ways to make cultural properties into tourist resources by considering the possibilities of place marketing using storytelling, based on an analysis of data gathered.

• Smart Structures and Systems
• /
• v.9 no.3
• /
• pp.207-230
• /
• 2012

Full-scale shake table seismic experiments and low-amplitude vibration tests on a masonry building are carried out to assess its seismic performance as well as study the effectiveness of a new multifunctional textile material for retrofitting masonry structures against earthquakes. The un-reinforced and the retrofitted with glass fiber reinforced polymer (GFRP) strips masonry building was subjected to a series of earthquake excitations of increasing magnitude in order to progressively induce various small, moderate and severe levels of damage to the masonry walls. The performance of the original and retrofitted building states is evaluated. Changes in the dynamic characteristics (lowest four modal frequencies and damping ratios) of the building are used to assess and quantify the damage states of the masonry walls. For this, the dynamic modal characteristics of the structure states after each earthquake event were estimated by performing low-amplitude impulse hammer and sine-sweep forced vibration tests. Comparisons between the modal results calculated using traditional accelerometers and those using Fiber Bragg Grating (FBG) sensors embedded in the reinforcing textile were carried on to investigate the reliability and accuracy of FBG sensors in tracking the dynamic behaviour of the building. The retrofitting actions restored the stiffness characteristics of the reinforced masonry structure to the levels of the original undamaged un-reinforced structure. The results show that despite a similar dynamic behavior identified, corresponding to reduction of the modal frequencies, the un-reinforced masonry building was severely damaged, while the reinforced masonry building was able to withstand, without visual damage, the induced strong seismic excitations. The applied GFRP reinforcement architecture for one storey buildings was experimentally proven reliable for the most severe earthquake accelerations. It was easily placed in a short time and it is a cost effective solution (covering only 20% of the external wall surfaces) when compared to the cost for full wall coverage by GFRPs.

• Journal of the Earthquake Engineering Society of Korea
• /
• v.25 no.5
• /
• pp.213-221
• /
• 2021

Many Korean domestic masonry structures constructed since 1970 have been found to be vulnerable to earthquakes because they lack efficient lateral force resistance. Many studies have shown that the brick and mortar suddenly experience brittle fracture and out-of-plane collapse when they reach the inelastic range. This study evaluated the seismic retrofitting of non-reinforced masonry with Hybrid Super Coating (HSC) and Cast, manufactured using glass fiber. Four types of specimen original specimen (BR-OR), one layered HSC (BR-HS-O), two-layered HSC (BR-HS-B), one layered HSC, and Cast (BR-CT-HS-O) were constructed and analyzed using compression, flexural tensile, diagonal compression, and triplet tests. The specimen responses were presented and discussed in load-displacement curves, maximum strength, and crack propagation. The compressive strength of the retrofit specimens slightly increased, while the flexural tensile strength of the retrofit specimens increased significantly. In addition, the HSC and Cast also produced a considerable increase in the ductile response of specimens before failure. Diagonal compression test results showed that HSC delayed brittle cracks between the mortar and bricks and resulted in larger displacement before failure than the original brick. The triplet test results confirmed that the bonding strength of the retrofit specimens also increased. The application of HSC and Cast was found to restrain the occurrence of brittle failure effectively and delayed the collapse of masonry wall structures.

• The Korea Journal of Herbology
• /
• v.33 no.4
• /
• pp.43-51
• /
• 2018

Objectives : Pharbitidis Semen, the seeds of Ipomoea nil (L.) Roth or I. purpurea (L.) Roth, is well-known traditional herbal medicine in Korea. But it is often marketed as a different seed or mixtures of its closely related species. Thus, the present study aims to provide external and micromorphological characters and identification key by using stereoscope (ST) and scanning electron microscope (SEM) for discriminating authentic of Pharbitidis Semen. Methods : A discrimination on external morphological characteristics of sepals, fruits, seeds, and hilum, testa cell micromorphology in the original plants and its congeneric species was carried out using digital calipers, ST, and SEM. Results : Number of valves (degree of apex of each valve), number of seeds per locule, hairy in capsules and size, luster, density of hairy, hilum shape in seeds and shape of cell, anticlinal, periclinal wall in testa may have high discriminative value. The seeds of Ipomoea nil as an original plant of Pharbitidis Semen were distinguished from other species by the relative larger in size, ovoid-trigonous in shape, mostly flabellate or triangular to trapezoid in outline (c.s.), dull, and puberulent in surface and thicken anticlinal wall. Conclusions : On the basis of the results, an identification key of Pharbitidis Semen and closely related species is provided. Our observations suggest that the combination of morphological characters and other studied results could be helpful in the successfully identified authentic herbal medicines. Moreover, micromorphological characters using SEM could be useful for discriminating authentic medicines.