• Journal of Acupuncture Research
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• v.23 no.2
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• pp.21-31
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• 2006

In the present study, We have investigated the bee venom (BV) and melittin (a major component of BV)-mediated anti-proliferative effects, and defined its mechanisms of action in cultured rat aortic vascular smooth muscle cells (VSMCs). BV and melittin $(0.4{\sim}0.8\;{\mu}g/ml)$ effectively inhibited 5% FBS-induced VSMCs proliferations. The regulation of apoptosis has attracted much attention as a possible means of eliminating excessively proliferating VSMCs. In the present study, the treatment of BV and melittin strongly induced apoptosis of VSMCs. These results suggest that the anti-proliferative effects of BV and melittin in VSMCs should be related with induction of apoptosis. Further study about Influence of BV and melittin upon apoptosis mechanism is therefor thought to be necessary to confirm the above results.

• Korean Journal of Acupuncture
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• v.17 no.1
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• pp.123-140
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• 2000

The purpose of this study is to understand the special character and the tendency of the movement change that 12 meridians possess. We studied about the incidental and fundamental rule of the six atmospheric influence that was presented by Pyobonjoong(the incidental, fundamental, intermediate) theory, and the Hwang won-uh's the Six Channel activity of the Qi theory that presented movement form of the meridian through the six atmospheric influence. Then we found the following substances. The Pyobonjoong(the incidental, fundamental, intermediate) theory is applicable to understand the special character and the tendency of the movement change that Meridians possess. But, because the Pyobonjoong(the incidental, fundamental, intermediate) theory can't classify the characteristic difference of the hand and foot meridians, then the principle that can devide the meridians of the hand and foot, must be supplied. The Jungwha(right changing)-Daewha(opposite changing) theory is able to concretely classify the special character of the hand and foot six meridians. And Hwang's Shawha(superintending change)-Jongwha(following change) theory that is base on Jungwha(right changing)-Daewha(opposite changing) theory, is able to classify the special character of the hand and foot six meridians, too. If the concept of the Meridians is understood by the Shawha(superintending change)-Jongwha(following change) theory, the special character of the hand and foot six meridians could be concretely classified and then the meridian of the Bowels and the six atmospheric influence corresponded to the meridians could be expansively explained as the point that take charge and control these special Qi in the human body The Bon-Qi(fundamental Qi) act on the special character of Shawha(superintending change) six meridians and the tendency of the movement change is cause by the insufficiency and excessiveness of the Bon-Qi(fundamental Qi). The Qi of the Shawha(superintending change) meridians act on the special character of Jongwha(following change) six meridians and the tendency of the movement change is cause by the exuberance of Yang and the deficiency of Yang.

• Herbal Formula Science
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• v.15 no.2
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• pp.9-19
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• 2007

Nineteen Medicaments of Mutual Antagonism currently belong to pharmaceutical incompatibility and some of them cannot be used in a same prescription: if they are used in a prescription, the treatment effect is rather reduced or toxic response may be produced. Therefore. inthisstudy, it was intended to look about how Nineteen Medicaments of Mutual Antagonism were defined through survey of literatures and to review the meaning and clinical potential. According to "Sinnongbonchogyeong," "Medicaments contain the substance that suppress toxins and the toxins may be removed with use of mutual restraint or mutual detoxication substances" and they have been used in terms of this concept. Since Tang and Song era, mutual restraint and mutual inhibition were confused and were difficult to be distinguished. In terms of pharmaceutical incompatibility, the original meaning of mutual restraint was deteriorated in "Sinnongbonchogyeong". That is. mutual restraint has been used as the concept of mutual inhibition or incompatibility. When various literatures were reviewed. it could be found that Nineteen Medicaments of Mutual Antagonism were firstly included in the phrases of songs and then in "seven emotion." It could be supposed that Nineteen Medicaments of Mutual Antagonism was created based on the clinical experiences of the author and the influence of doctors. Such supposition means indicates that the interactions among medicaments could effectively be applied and mutual restraint did not belong to pharmaceutical incompatibility. However. many doctors used mutual restraint and mutual inhibition in clinical practice with no distinguishment since Song era and. especially, it is supposed that. when medicaments were used with mixing. the pharmaceutical incompatibility of "Nineteen Medicaments of Mutual Antagonism" or "Eighteen Incompatible Medicaments" were emphasized and influenced on the efficacy of pharmaceutical preparations or acted as an obstacle in treating diseases. That is. an error was transferred: mutual restraint and mutual inhibition were not distinguished and were discretionally added or deleted through common people or professionals with no specific verification. The pharmaceutical preparations that belong to Nineteen Medicaments of Mutual Antagonism belong to pharmaceutical incompatibility but. when reviewed various literatures and clinical reports. they are not thought to be the ones that can never be used. Therefore. systematic literature review and experimental research should be performed.

• Natural Product Sciences
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• v.18 no.1
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• pp.60-66
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• 2012

Human skin is the first line of defense for the protection of the internal organs of the body from different stimuli. Ultraviolet B (UVB) irradiation induces skin damage and inflammation through the secretion of various cytokines, which are immune regulators produced by cells. To prevent the initiation of skin inflammation, keratinocytes that have been irreversibly damaged by radiation must be removed through the apoptotic mechanism. Ixeris dentata (family: Asteraceae) is a perennial medicinal herb indigenous to Korea. It has been used in Korea, China, and Japan to treat in digestion, pneumonia, diabetes, hepatitis, and tumors. To gain insight into the anti-inflammatory effects of I. dentata, we examined its influence on UVB-induced pro-inflammatory cytokine production in human keratinocytes (HaCaT cells), by observing cells that were stimulated with UVB in the presence or absence of I. dentata. In the present study, pro-inflammatory cytokine production was determined by performing enzyme-linked immunosorbent assay, reverse transcription polymerase chain reaction, and western blot analysis to measure the activation of mitogen-activated protein kinase (MAPKs). I. dentata inhibited UVBinduced production of the pro-inflammatory cytokine interleukin (IL)-6 in a dose-dependent manner. Further, I. dentata inhibited the UVB-induced expression of cyclooxygenase (COX)-2. Furthermore, I. dentata inhibited the phosphorylation of c-Jun NH2-terminal kinase and p38 MAPKs, suggesting that it inhibits the secretion of the pro-inflammatory cytokines IL-6 and IL-8, and COX-2 expression, by blocking MAPK phosphorylation. These results suggest that I. dentate can potentially protect against UVB-induced skin inflammation.

• Journal of Korean Medicine Rehabilitation
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• v.18 no.4
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• pp.73-83
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• 2008

Objectives : In this research, it was investigate that had effects on acupuncture techniques of $HT_{8}$(Shaofu) and $LR_{2}$(Xingjian) acupoints in high fat rats. We recorded data of weight gain, food intake, food efficiency, total cholesterol, triglyceride, AST, ALT, r-GTP, total bilirubin on rats fed high fat diet for 10 weeks. Methods : We divided experimental groups into four. Group of high-fat diet feeding and no treatment was control, group of high-fat diet feeding and acupuncture at $HT_{8}{\cdot}LR_{2}$ was Acu-1, group of high-fat diet feeding and acupuncture at $HT_{8}{\cdot}LR_{2}$ and rotated counter-clockwise 36times was Acu-2, high-fat diet feeding and acupuncture at $HT_{8}$(body direction)${\cdot}LR_{2}$(lower limb direction) and rotated counter-clockwise 36times was Acu-3. Results : In Acu-3 group, body weight on 2 weeks after starting acupuncture, food efficiency and the level of serum total cholesterol were decreased, and the level of HDL-cholesterol was increased. In Acu-1 group, the level of triglyceride was increased but ALT was decreased. Conclusions : These results suggest that treatment of acupuncture at $HT_{8}$(body direction)${\cdot}LR_{2}$(lower limb direction) and rotated counter-clockwise 36times may be having the influence on high fat by controlling the level of serum total cholesterol and HDL-cholesterol.

• Journal of Pharmacopuncture
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• v.19 no.3
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• pp.197-206
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• 2016

Introduction: This study aims to investigate the effects of acupuncture stimulation on the radial artery's pressure pulse wave, along with various hemodynamic parameters, and to explore the possible underlying mechanism of pulse diagnosis in healthy participants in their twenties. Methods and analysis: This study is a prospective, single-arm, exploratory clinical study. A total of 25 healthy participants, without regard to gender, in their twenties will be recruited by physicians. Written informed consent will be obtained from all participants. The participants will receive acupuncture once at ST36 on both sides. The radial arterial pulse waves will be measured on the left arm of the subjects by using an applicable pulse tonometric device (KIOM-PAS). On the right arm (appearing twice), electrocardiogram (ECG), photoplethysmogram (PPG), respiration and cardiac output (CO) signals, will be measured using a physiological data acquisition system (Biopac module), while the velocity of blood flow, and the diameter and the depth of the blood vessel will be measured using an ultrasonogram machine on the right arm (appearing twice). All measurements will be conducted before, during, and after acupuncture. The primary outcome will be the spectral energy at high frequencies above 10 Hz ($SE_{10-30Hz}$) calculated from the KIOM-PAS device signal. Secondary outcomes will be various variables obtained from the KIOM-PAS device, ECG, PPG, impedance cardiography modules, and an ultrasonogram machine. Discussion: The results of this trial will provide information regarding the physiological and the hemodynamic mechanisms underlying acupuncture stimulation and clinical evidence for the influence of acupuncture on the pressure pulse wave in the radial artery. Ethics and dissemination: This study was approved by the Institutional Review Board (IRB) of Kyung Hee University's Oriental Medical Center, Seoul, Korea (KOMCIRB-150818-HR-030). The study findings will be published in peer-reviewed journals and presented at national and international conferences. Trial registration number: This trial was registered with the Clinical Research Information Service (CRIS) at the Korea National Institute of Health (NIH), Republic of Korea (KCT0001663), which is a registry in the World Health Organization's (WHO's) Registry Network.

• The Journal of Pediatrics of Korean Medicine
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• v.21 no.3
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• pp.177-188
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• 2007

Objectives The purpose of this study is to investigate the effects of simultaneous treatment with supplementary therapy and treatment interval for improving symptoms and satisfaction rate by treating child rhinitis or paranasal sinusitis patients. Methods 41 rhinitis or paranasal sinusitis patients who visited the clinic between April 2004 and April 2006 were involved for this study. The patients were classified into Group A(2 sessions per week, simultaneous supplementary therapy), Group B(1 session in 2 weeks, simultaneous supplementary therapy) and Group C(1 session in 2 weeks, no supplementary therapy). After the experiment, the improvement rates of symptoms and satisfaction rate were surveyed by the questionnaires. Supplementary therapies used in Group A and B were aroma spray, Saengbit-patch, cutaneous acupuncture, nebulizer and Chuna manipulation. Results From 41 of the rhinitis or paranasal sinusitis patients, the Group A(simultaneous treatment group, 2 sessions of intensive care per week with supplementary therapy) showed the remaining symptoms score of $12.1{\pm}10.0$ in average. This score was the lowest comparing with the average of $25.7{\pm}12.9$ in Group B(1 session in 2 weeks, simultaneous treatment group with supplementary therapy) and the average of $21.0{\pm}20.1$ in Group C(1 session in 2 weeks, no supplementary therapy). Nose symptoms and sleep scores were significantly lower in Group A(p<0.01). The satisfaction score was the highest in Group B that had the most remaining symptoms and it had no significant relationship with the symptom improvement. Additional analysis showed that among many factors such as treatment interval, treatment method, cost, accessibility of the clinic, family history and satisfaction with the doctor; the satisfaction with their doctor was the most closely related to the satisfaction rate of the patients. Conclusions In this study with 41 of rhinitis or paranasal sinusitis patients, the 2 sessions of intensive care group showed the best results in improving symptoms. Supplementary treatments were also used simultaneously; aroma spray, cutaneous acupuncture, Saengbit-patch, Chuna manipulation and nebulizer. The questionnaires showed that the satisfaction rate of the patients(care taker) was not related to the symptom improvement. Additional analysis of the factors that influence the satisfaction rate showed that it was more closely related to the satisfaction with their doctors.

• The Journal of Internal Korean Medicine
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• v.22 no.3
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• pp.285-290
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• 2001

Purpose : Impaired glucose tolerance (IGT) was standardized in 1979 by the National Diabetes Data Group and the World Health Organization as a risk factor for type 2 diabetes, replacing groups such as 'borderline' and 'chemical' diabetes. The main clinical significance of IGT is as a risk factor for type 2 diabetes and as a risk factor for cardiovascular disease and as a component of the metabolic syndrome. In 1997 the American Diabetes Association(ADA) was proposed the new classification and diagnostic criteria for diabetes, which is strict with the diagnostic baseline of Diabetes from 140mg/dl to 126mg/dl. And it's main purpose is to prevent chronic complications by early diagnosis and treatments. In the oriental medicine, Supungsunkihwan has been used in treatments of Diabetes including IGT & NIDDM, however there is not enough studies about the its objective hypoglycemic effect. so in order to investigate whether there is hypoglycemic effect of Supungsunkihwan, clinical studies were performed with IGT and mild NIDDM patients. Methods : Prior to the study, fasting blood sugar(FBS) and postprandial 2hrs(PP2hrs) glucose were checked. In addition, ECG, cholesterol, TG, HbA1c levels were measured, and the dinical characteristics of patients that can be influence to the blood sugar level such as age, gender, rest type, diet type were surveyed. Supungsukihwan was administered for 7 days, and FBS & PP2hrs were measured again after the therapy. Result : PP2hrs glucose levels were decreased by the administration of Supungsunkihwan. And It's hypoglycemic effects has the correlation with age. That is, the more age increase, the more hypoglycemic effect decrease. Conclusion : Supungsukihwan has hypoglycmic effects on IGT & mild NIDDM patients and it is more effective when administrated to young patients relatively.

• Journal of Physiology & Pathology in Korean Medicine
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• v.19 no.5
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• pp.1129-1136
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• 2005

The writer reports the conclusions gained from study about the cause of the hypersensitive large intestine syndrome with Dongeuibogam as the central figure through researching the disharmony among Body Essence, Vital Energy, Mentality, and Blood, mutual action of five viscera and six bowels, and external shapes. The hypersensitive large intestine syndrome is generally chronic and recurred in many cases, so it is more efficacious than symptomatic to treat according to find the contradictions of individual shapes. The shapes and cases suffering frequently the hypersensitive large intestine syndrome are Gi-kwa and Sin-kwa, having a long nose, having a bruised spot on Triple warmer, man with inclined mouth, Taeeum type, man with congested fluids, man with colic symptoms. The hypersensitive large intestine syndrome in Oriental medicine is recognized of diarrhea, constipation, abdominal pain, abdominal distention and fullness caused by seven emotions. In Dongeuibogam it can be found out the similarity in depressive symptoms due to disorder of Gi, stagnation of Gi, dysphasia due to disorder of Gi, diarrhea due to disorder of Gi, fullness of due to Gi, diarrhea due to phlegm-retention, retention of undigested food, immoderate drinking, hypo-function of the spleen, or deficiency, abdominal pain from colic symptom, and difficulty in defecation and urination, internal injury, diarrhea due to weakness and fatigue. If the Jung, Gi, Sin, and Hyul composed the human body is broken harmony, the function of large intestinal transmission would be fallen, so similar symptoms like the hypersensitive large intestine syndrome are gotten. Especially Gi-kwa suffers diarrhea, constipation abdominal pain, and abdominal distention and fullness due to depressive symptoms from disorder of Seven emotions or Seven Gi. And Sin-kwa suffers from the hypersensitive large intestine syndrome due to emotional restlessness having an influence on rhythmic movement of abdomen. Examining between five viscera and six bowels and the hypersensitive large intestine syndrome, Liver cannot disperse well having influence on mutual relation of Liver-Large intestine, Heart reduces the function of defecation and urination not to control the seven emotions, Lung having exterior and interior relation with intestine has an influence on primordial energy and let the main symptoms occur, Spleen circulating the body fluid let the main symptoms occur due to malfunction of circulation, Kidney locating in lower part of the body has deep connection with intestine, so let the disorder. Urinary bladder is connected with intestine in moisture metabolism, Stomach is connected in receive and transmission, Small intestine is connected in absorption and excretion, from small intestine pain disturbing the abdominal movement, Samcho managing the catharsis of lower heater if declined its function causes the hypersensitive large intestine syndrome. The colic symptoms of Front private parts which disorder in lower abdomen give rise to abdominal pains, difficulty in defecation and urination due to Cold are similar to the hypersensitive large intestine syndrome. The treatments of applying the shapes of colic syndrome advocated by Master Park can be efficacious cure in clinic. Researching after the clinical cases of Master Park advocating Hyungsang medicine, we came to know that plenty of prescriptions of internal injury are applied and take good effects.

• Journal of Sasang Constitutional Medicine
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• v.23 no.3
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• pp.275-284
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• 2011

1. Objective: This literary review investigates Lee Jema's clinical experiences with Soyang-type patients and their influence on his conceptualization of the Soyang physiology and pathology. 2. Methods: 1) The case reports in "Soyang constitutional type: Spleen Cold-based Exterior-Cold disease" were compared before and after the Sinchuk revision to explore the temporal change in the Sasang medical concepts. 2) The texts in Donguisusebowon (Gabo edition) and Donguisusebowon (Sasangchobongwon) were analyzed to investigate the pathological concepts appearing before the completion of the Donguisusebowon (Gabo edition). 3) The texts in Donguisusebowon (Sinchuk edition) was analyzed to investigate the pathological concepts formed between the Gabo and Sinchuk editions of Donguisusebowon. 3. Results and Conclusions: 1) Gabo edition : The Gabo edition divided the External-origin Exterior disease into Cold-dominant (Heat-moderate) and Heat-dominant (Cold-moderate) patterns and differentiated the severity of Exterior-based Exterior disease and Interior-based Interior disease into mild, moderate, severe, and critical conditions. Cold-damage Delirium disease pattern was categorized as an Interior-based Interior disease, and the treatment protocol using Baekho-tang (Baihu-tang) was established. The stool condition and bowel movement reflecting the patient's defecation habits, the prime indicator of health in the Soyang constitutional type, were emphasized on their importance. 2) Sinchuk edition: The Delirium disease pattern was moved into the Exterior-cold disease, and the treatment protocol applying Jihwangbaekho-tang (Dihuangbaihu-tang) and Hyeongbangsabaek-san (Jingfangxiebai-san), with variational usage of Gypsum, was newly established. The Seong-Jeong and basal disease patterns were suggested as important factors in treating the patients. Also, it was proposed that the symptoms and signs reflecting the condition of the life-preserving energy be assessed to understand the patient's current condition. The importance of post-acute rehabilitation and aftercare as well as the most appropriate acute-stage treatment were emphasized.