• Advances in pediatric surgery
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• 제6권1호
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• pp.10-18
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• 2000

Multisystem organ failure resulting from gram negative bacterial sepsis is associated with high morbidity and mortality in surgical neonates. There are differences in the clinical characteristics of organ failure in neonates and adults. The purpose of this study is to identify the differences and determine the order of organ failure between baby rats and adult rats after induction of gram negative sepsis. Fifty baby rats less than 30-day-old and another 50 adult rats more than 2-month-old were divided into control group (G1) and experimental group (G2). The G1 consisted of 10 baby- and 10 adult-rats, and the G2 consisted of 40 babies and 40 adults. E. coli ($10^8/mL$ per 100g of body weight) were injected into the peritoneal cavity in G2 and same amount of saline was injected in G 1. Blood samples were obtained before injection, 24 hour, 48 hour, 72 hour and after death. WEC, platelet, $PaO_2$, $PaCO_2$, total bilirubin, BUN, creatinine, albumin and abdominal wall thickness were measured to evaluate the sequence of organ failure. The mortality was 55.0 % in G2-babies and 32.5 % in G2-adults. In baby rats, microvascular, hematologic and renal failure appeared within 24 hours after injection and pulmonary failure followed. Pulmonary, renal and liver failure developed within 24-48 hours in adult rats; however, microvascular failure did not appear until they were moribund. Thrombocytopenia, hypoalbuminemia, increased BUN and generalized edema was the earlist sign of sepsis in baby rats.

• Journal of Radiation Protection and Research
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• 제30권4호
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• pp.197-208
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• 2005

Although little information is available on the underlying mechanisms, various genetic factors have been associated with tissue-specific responses to radiation. In the present study, we explored the possibility whether organ specific gene expression is associated with radiosensitivity using samples from brain, lung and spleen. We examined intrinsic expression pattern of 23 genes in the organs by semi-quantitative RT-PCR method using both male and female C57BL/6 mice. Expression of p53 and p21, well known factors for governing sensitivity to radiation or chemotherapeutic agents, was not different among the organ types. Both higher expression of sialyltransferase, delta7-sterol reductase, leptin receptor splice variant form 12.1, and Cu/Zn superoxide dismutase (SOD) and lower expression of alphaB crystalline were specific for spleen tissue. Expression level of glutathione peroxidase and APO-1 cell surface antigen gene in lung tissue was high, while that of Na, K-ATPase alpha-subunit, Cu/ZnSOD, and cyclin G was low. Brain, radioresistant organ, showed higher expressions of Na, K-ATPase-subunit, cyclin G, and nucleolar protein hNop56 and lower expression of delta7-sterol reductase. The result revealed a potential correlation between gene expression patterns and organ sensitivity, and Identified genes which might be responsible for organ sensitivity.

• Asian-Australasian Journal of Animal Sciences
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• 제32권3호
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• pp.341-349
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• 2019

Objective: Internal organs indirectly affect economic performance and well-being of animals. Study of internal organs during later layer period will allow full utilization of layer hens. Hence, we conducted a genome-wide association study (GWAS) to identify potential quantitative trait loci or genes that potentially contribute to internal organ weight. Methods: A total of 1,512 chickens originating from White Leghorn and Dongxiang Blue-Shelled chickens were genotyped using high-density Affymetrix 600 K single nucleotide polymorphism (SNP) array. We conducted a GWAS, linkage disequilibrium analysis, and heritability estimated based on SNP information by using GEMMA, Haploview and GCTA software. Results: Our results displayed that internal organ weights show moderate to high (0.283 to 0.640) heritability. Variance partitioned across chromosomes and chromosome lengths had a linear relationship for liver weight and gizzard weight ($R^2=0.493$, 0.753). A total of 23 highly significant SNPs that associated with all internal organ weights were mainly located on Gallus gallus autosome (GGA) 1 and GGA4. Six SNPs on GGA2 affected heart weight. After the final analysis, five top SNPs were in or near genes 5-Hydroxytryptamine receptor 2A, general transcription factor IIF polypeptide 2, WD repeat and FYVE domain containing 2, non-SMC condensin I complex subunit G, and sonic hedgehog, which were considered as candidate genes having a pervasive role in internal organ weights. Conclusion: Our findings provide an understanding of the underlying genetic architecture of internal organs and are beneficial in the selection of chickens.

• 대한방사선기술학회지:방사선기술과학
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• 제45권4호
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• pp.331-339
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• 2022

The purpose of this study was to evaluate maternal organ and fetal doses by week of pregnancy for pregnant women nuclear medicine practitioners in the nuclear medicine field. In addition, we intend to present basic data for the management of exposure doses of female nuclear medicine practitioners. In this study, phantoms of childbearing women, 3, 6, 9 months pregnant women were simulated using MCNPX(Monte Carlo N-Particle Extended) among the Monte Carlo methods. First, volume source was constructed based on 10 cm of the anterior part of the lower abdomen of the phantom, and the organ and fetal doses were evaluated for each week of the pregnant woman according to the type of radioactive isotope. Second, the organ and fetal dose of pregnant women were evaluated by increasing the distance between the source and the abdominal surface by 50 and 100 cm. As a result, ¹⁸F sources showed high organ and fetal doses in pregnant women 0 to 3 months, and the dose distribution gradually decreased in 6 to 9 months pregnant women. The distribution of organ and fetal doses for ⁹⁹mTc and ¹²³I sources showed the same tendency as that of ¹⁸F, and the overall absorbed dose distribution was relatively lower than that of ¹⁸F. Through this study, it is considered that workers in the early stages of pregnancy within 3 months will need appropriate management to minimize occupational exposure dose.

• 한국수산과학회지
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• 제26권1호
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• pp.76-90
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• 1993

징거미새우의 생식소 성숙 제어에 대한 광주성발달과 안병 X-organ과의 관계를 밝히기 위하여 1) 생식소 성숙 제어에 미치는 수온과 광주기의 영향, 2)생식소 성숙에 대한 안병 절제 효과, 3) 안병에 있는 외수 X-organ (MEX-organ)조직의 활성에 대한 계절적인 변화 등을 조사하였다. 전난황형성기(12월${\sim}$다음해 3월)와 난황형성기(4월${\sim}$5월)에 있어서, 생식소 성숙은 주로 수온에 영향을 받으며, 광주조건(12L/12D, 15L/9D)에 관계없이 수온상승에 의하여 GSI가 증가하고 생식소도 성숙하였다. 반면, 산란기(6월${\sim}8$월)에 있어서, 생식소 성숙은 주로 광주조건에 영향을 받으며, 15L/9D와 같은 장일 조건에서는 생식소 성숙이 지속되고 높은 GSI값을 나타내는 반면, 12L/12D와 같이 단일화된 조건에서는 난소내에 있는 대부분의 난황형성기 난모세포들은 퇴화${\cdot}$흡수되어 휴지기 상태의 난모세포로 되었고, GSI는 감소되었다. 휴지기(9월${\sim}11$월)에 있어서, 수온($16^{\circ}C,\;22^{\circ}C,\;28^{\circ}C$)과 광주조건(12L/12D, 15L/9D)을 조합한 모든 실험구에서는 재성숙이 일어나지 않았다. 생식소 성숙에 대한 안병 절제 효과는 전난황형성기와 산란기때 보다 휴지기 때가 더 강하게 나타났다. 안병 MEX-organ에서 직경이 $20{\mu}m$이상인 신경분비세포 수는 생식주기에 따라 변동을 보였으며, 휴지기 때 그 수는 $77{\pm}12$로 증가하였고, 난황형성기때는 $55{\pm}7$로 감소하였다. 그러나 Bellonci 조직의 크기(volume)는 휴지기 때에 비해서 난환형성기때 증가하는 경향을 보였다.

• Journal of Hospice and Palliative Care
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• 제9권1호
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• pp.11-16
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• 2006

목적: 본 연구는 간호사를 대상으로 뇌사자 장기 기증에 대한 태도와 태도도구 항목의 요인분석을 실시하여 뇌사자 장기기증 활성화를 위한 기초자료를 마련하고, 뇌사자 장기기증 활성화를 위한 방법을 모색할 목적으로 실시하였다. 방법: B광역시에 위치하고 있는 3개의 대학병원과 4개의 종합병원에 근무하는 간호사 198명을 대상으로 조사한 서술적 조사연구이다. 기관의 부서장을 만나 연구의 목적과 필요성을 설명하여 허락을 받고, 병동 수간호사에게 연구의 취지와 목적을 다시 설명하고 협조를 구한 후, 해당 병동 간호사의 동의를 얻어 구조화된 설문지를 배부하여 설문조사 하였다. 결과: 대상자의 뇌사자 장기기증에 대한 태도도구 항목의 요인은 긍정적 태도가 '뇌사의 법적 사망 인정' 요인, '뇌사시 장기기증 의사' 요인, '학교교육과정에 포함' 요인, '타인에 대한 선물' 요인, '전문단체와의 연결'요인, '뇌사인정은 꼭 필요' 요인, '국가적으로 제도화' 요인, '장기이식을 위해 좋은 일' 요인으로 나타났고, 부정적 태도는 '종교와 생명의 존엄성에 위배' 요인, '뇌사인정 시 위험성' 요인, '의료진에 대한 불신감' 요인, '장기 기증 권유의 부담'요인, '경제적 보상'요인으로 나타났다. 대상자의 뇌사자 장기기증에 대한 긍정적 태도정도는 총 평균평점이 3.75점, 표준편차가 3.40로 중간 정도로 나타났다. 각 요인별 긍정적 태도정도는 '학교교육과정에 포함' 요인이 가장 높았고($4.15{\pm}0.54$), 긍정적 태도정도가 가장 낮은 요인은 '뇌사시 장기기증 의사' 요인($3.27{\pm}0.56$)이었다. 그리고 대상자의 뇌사자 장기기증에 대한 부정적 태도정도는 총 평균평점이 2.92점, 표준편차가 0.47로 중간 정도로 나타났다. 각 요인별 긍정적 태도정도는 '장기기증 권유의 부담' 요인($3.11{\pm}0.58$)이 가장 높았고, 부정적 태도정도가 가장 낮은 도인은 '종교와 생명의 존엄성에 위배' 요인($2.50{\pm}0.64$)으로 나타났다. 결론: 뇌사자 장기 기증에 대한 간호사의 수용적인 태도 전환을 위해 간호대학 교육과정에 이에 관한 교육내용이 포함되어야 하고 또한 졸업간호사들을 위한 보수교육과정에도 이를 포함시켜야 할 필요성이 있으며, 이는 뇌사 입법화 후 감소추세에 있는 장기 이식과 뇌사자 장기 기증에 관한 태도의 변화를 가져오는데 기여할 수 있을 것으로 사료된다.

• Parasites, Hosts and Diseases
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• 제54권1호
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• pp.21-29
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• 2016

The sodium hydrogen exchanger 1 (NHE1), which functions in maintaining the ratio of $Na^+$ and $H^+$ ions, is widely distributed in cell plasma membranes. It plays a prominent role in pH balancing, cell proliferation, differentiation, adhesion, and migration. However, its exact subcellular location and biological functions in Toxoplasma gondii are largely unclear. In this study, we cloned the C-terminal sequence of T. gondii NHE1 (TgNHE1) incorporating the C-terminal peptide of NHE1 (C-NHE1) into the pGEX4T-1 expression plasmid. The peptide sequence was predicted to have good antigenicity based on the information obtained from an immune epitope database. After induction of heterologous gene expression with isopropyl-b-D-thiogalactoside, the recombinant C-NHE1 protein successfully expressed in a soluble form was purified by glutathione sepharose beads as an immunogen for production of a rabbit polyclonal antiserum. The specificity of this antiserum was confirmed by western blotting and immunofluorescence. The antiserum could reduce T. gondii invasion into host cells, indicated by the decreased TgNHE1 expression in T. gondii parasites that were pre-incubated with antiserum in the process of cell entry. Furthermore, the antiserum reduced the virulence of T. gondii parasites to host cells in vitro, possibly by blocking the release of $Ca^{2+}$. In this regard, this antiserum has potential to be a valuable tool for further studies of TgNHE1.

• 대한수의학회지
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• 제28권1호
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• pp.145-154
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• 1988

제주도 남제주군 성산읍 성산리 성산일출봉 부근에서 생포된 천연기념물(天然記念物) 보호조인 흰꼬리 수리로 부터 Ixodes signatus 약충을 채취하여 생리학적(生理學的) 주요감각기관(主要感覺器官)에 대(對)한 표면미세구조(表面微細構造)를 주사(走査) 전자현미경(電子顯微鏡)으로 관찰(觀察)하였든 바, 영상관찰(映像觀察) 및 사진분석(寫眞分析) 결사(結査) 얻어진 소견(所見)을 요약(要約)하면 다음과 같다. 1. 충체(蟲體) 배면(背面) 및 복면(腹面)에 대(對)한 관찰(觀察)에서 지문상(指紋狀)의 수많은 표면(表面)주름과 다양(多樣)한 크기의 강모(剛毛)들을 볼수 있었으며, 특(特)히 구기부(口器部)에 있어서 크고 잘 발달(發達)된 강모(剛毛) 또는 강모군(剛毛群)을 확인(確認)할 수 있었다. 2. 복면하부(腹面下部)의 항문(肛門)과 항문(肛門)주위에 위로부터 아래로 뻗어내린 항문구(肛門溝)가 뚜렷이 패여져 있었으며 1쌍(雙)으로된 항문엽(肛門葉)에는 각각(各各) 3개씩의 감각성(感覺性) 강모(剛毛)가 위치(位置)하고 있음을 확인(確認)하였다. 3. 측면(側面)에 부착되어 있는 기문판(氣門板) 관찰(觀察)에서 중앙부(中央部)에 단추모양의 구조물(構造物)이 있었으며 변연부(邊緣部)에는 수많은 기공(氣孔)들이 뚫려 있음을 확인(確認)하였다. 4. 사지(四肢)에 대(對)한 관찰결과(觀察結果), 기절(基節)의 외측돌기(外側突起)가 특징적으로 관찰(觀察)되었으며, 각(各) 마디 표면(表面)의 많은 강모(剛毛)와 제1지(第1肢)의 부절에서 화학수용기(化學受容器)의 기능(機能)을 갖고 있는 홀러씨 기관(Haller's organ)을 관찰(觀察)하였다. 5. 타원형(楕圓型)으로 열려있는 홀러씨 기관 주변에서 2종류(種類) 크기의 강모군(剛毛群)을 관찰(觀察)할 수 있었으며, 기관내부(器官內部)에는 갈쿠리 모양의 돌출형(突出型) 감각성(感覺性) 간상체 7개가 정렬되어 있음을 확인(確認)할 수 있었다.

• Reproductive and Developmental Biology
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• 제31권2호
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• pp.83-90
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• 2007

Insulin-like growth factor II (IGF2) and H19 genes are mutually imprinted genes which may be responsible for abnormalities in the cloned fetuses and offspring. This study was performed to identify putative differentially methylated regions (DMRs) of porcine H19 locus and to explore its genomic imprinting in in vitro fertilized (IVF) and somatic cell nuclear transferred (SCNT) embryos. Based on mice genomic data, we identified DMRs on H19 and found porcine H19 DMRs that included three CTCF binding sites. Methylation patterns in IVF and SCNT embryos at the 2-, 4-, $8{\sim}16$-cells and blastocyst stages were analyzed by BS (Bisulfite Sequencing)-PCR. The CpGs in CTCF1 was significantly unmethylated in the 2-cell stage IVF embryos. However, the 4- (29.1%) and $8{\sim}16$-cell (68.2%) and blastocyst (48.2%) stages showed higher methylation levels (p<0.01). On the other hand, SCNT embryos were unmethylayted ($0{\sim}2%$) at all stages of development. The CpGs in CTCF2 showed almost unmethylation levels at the 2-,4- and $8{\sim}16$-cell and blastocyst stages of development in both IVF ($0{\sim}14.1%$) and SCNT ($0{\sim}6.4%$) embryos. At all stages of development, CTCF3 was unmethylated in IVF ($0{\sim}17.3%$) and SCNT ($0{\sim}1.2%$) embryos except at the blastocyst stage (54.5%) of IVF embryos. In conclusion, porcine SCNT embryos showed an aberrant methylation pattern comprised to IVF embryos. Therefore, we suggest that the aberrant methylation pattern of H19 loci may be a reason for increased abnormal fetus after embryo transfer of porcine SCNT embryos.

• Journal of Gastric Cancer
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• 제12권2호
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• pp.81-87
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• 2012

Purpose: The aim of the present study was to assess the association between the pre-operative plasma fibrinogen level and the adjacent organ involvement in advanced gastric cancer. Materials and Methods: A total of 923 pre-operative plasma samples were obtained from 923 patients diagnosed clinically as having advanced gastric cancer, and fibrinogen levels were measured by immunoassay. Associations between fibrinogen levels and clinicopathologic findings (depth of tumor, adjacent organ involvement, and lymph node metastasis), along with survival were examined by univariate and multivariate analyses. Results: Tumor size, tumor depth, and the presence of lymph node metastasis were found to be positively correlated with the preoperative plasma fibrinogen levels (P<0.001). Fifty (5.4%) patients had adjacent organ involvement. Lymphatic invasion (P<0.001), tumor size (P<0.001), clinical T (depth of invasion) stage (P<0.001), and clinical nodal stage (P=0.018) were found to be associated with adjacent organ involvement. Univariate and multivariate regression analyses showed that a preoperatively elevated plasma fibrinogen level was associated with adjacent organ involvement (P<0.001, 0.028), and Kaplan-Meier analysis showed that it was associated with poorer survival (P<0.001). Conclusions: Plasma fibrinogen was found to be a clinically useful marker of adjacent organ involvement and overall survival. When a high fibrinogen level is encountered, preoperatively, adjacent organ involvement should be suspected in clinically advanced gastric cancer.