• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 추계학술발표대회 및 bio-venture fair
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• pp.273-276
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• 2000

Marine fungus, Thraustochytrium aureum ATCC 34034, was incubated in artificial sea water media in order to produce docosahexaenoic acid(DHA). Cultures were performed at $24^{\circ}C$ in light for 13 days with orbital shaker at 100rpm. Maximum cell productivity of 1.34g/L and DHA yield of 41.4 mg/L were obtained by using this method, which is almost the twice level of DHA yield obtained for the strains reported previously. This strain did produce much more DHA after sporangium disruption.

• Journal of Microbiology and Biotechnology
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• 제21권12호
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• pp.1299-1305
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• 2011

An important modification of thrombolytic agents is resistance to plasminogen activator inhibitor-1 (PAI-1). In previous studies, a new truncated PAI-1-resistant variant was developed based on deletion of the first three domains in t-PA and the substitution of KHRR 128-131 amino acids with AAAA in the truncated t-PA. The novel variant expressed in a static culture system of Chinese Hamster Ovary (CHO) DG44 cells exhibited a higher resistance to PAI-1 when compared with the full-length commercial drug; Actylase. In the present study, the truncated-mutant protein was expressed in CHO DG44 cells in 50 ml orbital shaking bioreactors. The final yield of the truncated-mutant in the culture was 752 IU/ml, representing a 63% increase compared with the static culture system. Therefore, these results suggest that using the combined features of a transient and stable expression system is feasible for the production of novel recombinant proteins in the quantities needed for preclinical studies.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 춘계학술발표대회
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• pp.291-294
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• 2000

Thraustochytrium aureum ATCC 34034를 인공 해수 배지에서 $24^{\circ}C$, 200rpm, 초기 pH 6으로 고정하여 빛을 가하면서 3일간 배양을 한 결과, 최대 건조 균체량은 1.34g/L이었으며, 최대 DHA생산량은 41.4mg/L이었다. 모든 실험에서 탄소원으로 glucose를 5g/L로 고정하였다. YM배지와 YPG배지에서는 Thraustochytrium aureum이 DHA를 생산하지 못하였으며, 이 균주가 해양 미생물이라는 점을 고려해보면, 성장과 DHA생산에 있어서 염분이 중요한 영향을 미친다고 생각된다.

• 한국식품영양학회지
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• 제13권4호
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• pp.328-333
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• 2000

Aspergillus niger KCTC 6144 포자를 alginate bead로 고정화하여 호박배지(포도당 9%, 호박가루 1%, pH 6)를 이용하여 구연산 발효를 수행하였다. 고정화된 A. niger 포자가 bead 내에서 발아하여 균사가 bead를 뚫고 자라났으며 3$0^{\circ}C$에서 4일간 배양 후 그 크기는 2.0~2.5mm에서 6~8mm로 커졌다. 3$0^{\circ}C$에서 5일간 진탕배양(150rpm)으로, 50ml 호박 배지가 든 250ml 삼각 flask를 사용하여, 구연산 생성의 최적 발효 조건을 검토한 결과는 다음과 같다. 탄소원으로 포도당을 12%로 올렸을 때 구연산의 생성이 최고에 달하였고, 질소 및 무기질원으로 1%의 호박가루가 구연산 생성에 가장 알맞는 농도였다. 초기 pH는 6.0, bead의 수가 100개로 하였을 때 구연산 생성이 가장 좋았으며, 포도당 12%, 호박가루 1%로 한 최적 배양 조건에서, 5일만에 구연산이 23.5g/ι로 최고로 생성되었다.

• Journal of Microbiology and Biotechnology
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• 제28권9호
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• pp.1511-1516
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• 2018

In this study, we investigated Indole-3-acetic acid (IAA) production by a rice phylloplane bacteria, Enterobacter sp. DMKU-RP206, using sweet whey as a feed stock instead of lactose. We succeeded in using sweet whey for Enterobacter sp. DMKU-RP206 to produce 3,963.0 mg IAA/l with the optimal medium containing 1.48% sweet whey, 1.42% yeast extract and 0.88% $\text\tiny{L}$-tryptophan. The medium pH was adjusted to 6 and the culture conditions were shaking at 200 rpm on an orbital shaker at $30^{\circ}C$ for 3 days. We also evaluated the effect of IAA in culture filtrates of Enterobacter sp. DMKU-RP206 on the promotion of jasmine rice growth in a pot experiment. Compared with the negative control (without IAA), the result showed that biosynthetic IAA produced by Enterobacter sp. DMKU-RP206 significantly increased the growth of jasmine rice (Oryza sativa L. cv. KDML105) in terms of length and dry weight of shoot. This work thus reveals the impact of IAA produced by Enterobacter sp. on the promotion of jasmine rice growth.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2018년도 추계학술대회
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• pp.109-109
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• 2018

Bacillus subtilis B22, a chemotrophic and aerobic bacterial strain was isolated from homemade kimchi, identified by 16S rRNA gene sequencing. B22 was primarily screened by biochemical, carbon source utilization tests. B22 was used to produce pectinase and ${\beta}$-glucosidase by submerged fermentation under different light sources. B22 was incubated in pectin media and basal media (pH 7.0) under blue, green, red and white light-emitting diodes (LEDs), fluorescent white light, and in darkness at $37^{\circ}C$, orbital shaker 150 rpm for 24 hours. Fermentation under blue LEDs maximized pectinase production ($71.59{\pm}1.6U/mL$ at 24 h) and ${\beta}$-glucosidase production ($56.31{\pm}1.6U/mL$ at 24 h). Further, the production of enzyme increased to pectinase ($156{\pm}1.28U/mL$) and ${\beta}$-glucosidase ($172{\pm}1.28U/mL$) with 3% glucose as a carbon source. Activity and stability of the partially purified enzymes were higher at pH 6.0 to 8.0 and $25-55^{\circ}C$. The effect on the metal ions $Na^+$ and $K^+$ and (moderateactivity) $Mn^{2+}$ and $Ni^{2+}$ increased activity, while $Hg^{2+}$, $Cu^{2+}$, $Fe^{2+}$, and $Fe^{2+}$ inhibited activity. EDTA, phenylmethylsulfonyl fluoride and 5,5-dithiobis (2-nitrobenzoicacid) reduced activity, while tetrafluoroethylene and 1,10-phenanthroline inhibited activity. The amylase was highly tolerant of the surfactants TritonX-100, Tween-20, Tween-80 and compatible with organic solvents methanol, ethanol, isoamylalcohol, isopropanol, t-butylalcohol and the oxidizing agents hydrogen peroxide, sodium perborate and sodium hypochlorite, although potassium iodide and ammonium persulfate reduced activity. These properties suggest utility of pectinase and ${\beta}$-glucosidase produced by B. subtilis B22 under blue LED-mediated fermentation for industrial applications.

• 한국임상수의학회지
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• 제21권1호
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• pp.20-22
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• 2004

개의 아토피성 피부염의 주된 세균성 감염은 Staphylococcus intermedius에 의해 발생한다. 본 연구의 목적은 아토피성 피부염을 가지고 있는 환견의 혈청과 정상견의 혈청을 이용하여 체액성 면역반응을 유발하는 주된 세균단백질을 규명하는데 있다. 건국대학교 수의과대학 부속동물병원에 내원한 아토피성 피부염 및 표재성 세균성 농피증을 앓고 있는 환견의 혈청 및 정상견의 혈청을 분리하여 본 실험에 사용하였으며 아토피성 피부염을 가지고 있는 환견에서 S. intermedius 균을 분리하였다. Brain heart infusion 액체배지 조건 및 $37^{\circ}C$ 배양조건에서 호기상태로 증균시켰으며 증균후 포도상구균을 PBS완충액에 부유시킨후 원심분리하고 최종적으로 lysostaphin을 이용하여 세균단백질을 분리하였다. 수거한 세균단백질은 SDS-PAGE을 이용하여 단백질을 전기영동하였으며 영동후 nitrocellulose membrane을 이용하여 단백질을 이동시켰다. Western blot은 anti-dog-IgG, 아토피성 피부염의 혈청 및 정상혈청을 이용하였다. 결론적으로 아토피성 피부염의 혈청을 이용해 확인한 S. intermedius의 주요 세균단백질은 18, 31, 75, 및 110 kDa이였다. 현재의 연구결과로 볼 때 아토피성 피부염에 감염된 대부분의 환견은 S. intermedius의 세균단백질에 대한 체액성 면역반응이 유발된다고 생각된다.