• Molecules and Cells
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• v.19 no.3
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• pp.391-397
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• 2005

Molecular identification techniques are used where morphological characters are not useful for distinguishing species that resemble each other closely. The example studied here is the Adoxophyes species complex, in which A. orana (Fischer von $R{\ddot{o}}sslerstamm$) is officially the only known Korean species in the genus Adoxophyes (Lepidoptera: Tortricidae). However there have been suspicions that at least two types of A. orana exist in Korea based on the distribution and range of the host, with A. orana attacking apples and peaches, and another Adoxophyes sp. attacking tea and pears. The latter is presumed to be A. honmai (Yasuda), but the two have remained confused because of their extreme morphological similarity, despite several Asian studies of pheromonal and morphological characteristics. To confirm the occurrence of an Adoxophyes species other than A. orana in Korea, we compared 940 bp of the mitochondrial cytochrome oxidase I (COI) gene from 16 samples of Adoxophyes and found that there is a second Adoxophyes species different from A. orana. Comparison of the different sequences to that of Japanese A. honmai confirmed that they belong to the latter. From the sequence difference between the two Korean species, we were able to develop new PCR primer sets that distinguish them. This molecular identification technique with no enzyme digestion or sequencing step is a convenient and rapid way of differentiating between species that are hard to distinguish morphologically.

• Animal cells and systems
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• v.16 no.2
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• pp.154-161
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• 2012

We reviewed the genus $Adoxophyes$ (Lepidoptera: Tortricidae) from Korea and recognized three species of the genus. Among them, $A.$ $paraorana$ sp. nov., formerly misidentified as $A.$ $orana$ in Korea, is described as new to science. $Adoxophyes$ $orana$, a notorious pest known in most Eurasian countries for a long time, turns out to be only recently introduced or found in Korea. Photographs of the adults and genitalia of the species are provided. Specific distinction was supported by the COI barcode study.