• Journal of Periodontal and Implant Science
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• 제27권4호
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• pp.867-882
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• 1997

Safflower(Carthamus tinctorius $Linn\acute{e}$ has been traditionally used for the treatment of blood stasis, and Dipsasi Radix has been used as a drug for fracture in Chinese medicine. The purpose of present study was to examine the biologic effects of safflower extract and Disasi radix extracts on the periodontal. ligament cells and osteoblastic cells and on the wound healing of rat calvarial defect. The ethanolic extract of safflower blossom, safflower seed and Dipsasi Radix(125, 250, and 500 ${\mu}g/ml$) were prepared as test group, and PDGF-BB(lOng/ml) and unsafonifiable fraction of Zea Mays L.(125, 250, and 500 ${\mu}g/ml$) were employed as positive control. The effects of each agents on the growth and survival, ALPase activity, expression of PDGF-BB receptor, chemotactic response of PDL cell and ATCC human osteosarcoma MG63 cells in vitro were examined. The tissue regenerative effect of each extracts was evaluated by histomorphometric measuring of newly formed bone on the 8mm defect in rat calvaria after oral administration of 3 different dosages groups : 0.02, 0.1 and 0.35g/kg, per day. It was also employed the same dosages of unsaponifiable fraction of Zea Mays L. as positive controls. Safflower blossom extract, safflower seed extract, and Dipsasi Radix extract stimulate the cellular activity of MG63 cells in concentration range of $125-500{\mu}g/ml$, and safflower bolssom extract and safflower seed extract stimulate also the cellular activity of periodontal ligament cells in concentration range of $250-500{\mu}g/ml$. In activity of ALPase, $250-500{\mu}g/ml$ of safflower blossom extracts showed significant stimulating effects on MG63 cells, and the same concentration range of safflower seed extracts showed significant effect on periodontal ligament cells. In the recovery on PDGF-BB receptor expression which was depressed by $IL-1{\beta}$, $125-250{\mu}g/ml$ of safflower blossom extracts and $250-500{\mu}g/ml$ of safflower seed extracts showed significant increasing effect on MG63 cells, and $500{\mu}g/ml$ of safflower blossom extract and $250-500{\mu}g/ml$ of safflower seed extracts showed significant effect on periodontal ligament cells. In chemotactic response, among all tested group, safflower seed extracts only were chemotactic to MG63 cells and periodontal ligament cells in concentration range of $125-500{\mu}g/ml$. Also in the view of bone regeneration in rat calvarial defect model, the only group that was orally administrated 0.35g/kg, day of safflower seed extract showed significant new bone formation. These results suggested that safflower extracts might have a potential possibilities as an useful drug for adjunct to treatment for regeneration of periodontal defect.

• 치위생과학회지
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• 제7권3호
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• pp.179-185
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• 2007

본 연구는 대구광역시에 소재한 인문계, 실업계 남자 고등학생들을 대상으로 청소년기의 측두하악장애 유병실태와 기여요인, 악안면외상 경험과 관리 실태, 고교별 측두하악장애와 학교생활에서의 스트레스와의 관계를 관찰함으로서 측두하악장애의 예방과 치료에 있어 적절한 인식과 구강보건교육프로그램개발에 필요한 기초 자료를 마련하고자 본 연구를 시도하여 다음과 같은 결과를 얻었다. 1. 남자고등학생에서 측두하악장애의 유병률은 61.8%에서 관절잡음을 경험하였고, 관절탈구 6.9%, 저작시동통 47.5%, 비저작시동통 29.8%, 개구장애 11.3%, 두통이 40.4%로 나타났다. 2. 측두하악장애를 일으키는 기여요인에서 관절잡음은 이악물기, 입술 뺨깨물기, 저작시동통에서는 이악물기, 이갈이, 편측저작, 과잉저작, 입술 뺨깨물기, 모로취침 등이 유의한 차이를 보였다(P < 0.01). 비저작시동통은 이악물기, 이갈이, 편측저작, 입술 뺨깨물기 개구장애는 이악물기, 이갈이, 모로취침에서 통계적으로 유의한 차이를 보였다(P < 0.01). 두통은 모로취침을 제외한 나머지 항목에 대한 악습관이 측두하악장애의 기여요인으로 나타났다(P < 0.01, P < 0.05). 3. 측두하악장애를 일으킬 수 있는 악안면외상 경험률은 인문계고 19.6%, 실업계고 13.4%였고, 외상 요인으로 인문계고는 운동 26.8%, 기타 24.4%, 넘어짐 19.5%순이였으며, 실업계고는 운동 44.4%, 넘어짐 22.2%, 기타 14.9%순이었다. 치료경험은 인문계고 있다 2.9%, 실업계고 5.0%로 나타났으며 치료기관으로는 인문계고 치과 50%, 정형외과 50%, 실업계고 정형외과 40%, 한의원 30%, 치과 30%로 나타났다. 4. 측두하악장애는 학년별, 학력수준별 유의한 차이는 없었고, 인문계고와 실업계고의 측두하악장애 정도에서 저작시동통, 비저작시동통은 유의한 차이를 보였다(P < 0.01). 5. 고교별 학교에서의 스트레스를 비교해보면 성적 및 학업문제로 인해 인문계고 $3.75{\pm}1.14$, 실업계고 $3.01{\pm}1.23$로 전반적으로 실업계고 보다는 인문계고에서 스트레스 정도가 높게 나타나 유의한 차이를 보였다. 6. 성적 및 학업의 문제, 학교생활문제, 교사와의 문제로 인한 스트레스와 측두하악장애의 저작시동통, 비저작시동통, 관절잡음은 유의한 상관관계를 보였다(P < 0.01, P < 0.05).

• 대한구강악안면임플란트학회지
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• 제22권4호
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• pp.242-254
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• 2018

The aim of the current study was to evaluate the results of horizontal guided bone regeneration (GBR) with xenograf t (deproteinized bovine bone mineral, DBBM), allograf t (irradiated allogenic cancellous bone and marrow), titanium membrane, resorbable collagen membrane, and advanced platelet-rich fibrin (A-PRF) in the anterior maxilla. The titanium membrane was used in this study has a three-dimensional (3D) shape that can cover ridge defects. Case 1. A 32-year-old female patient presented with discomfort due to mobility and pus discharge on tooth #11. Three months after extracting tooth #11, diagnostic software (R2 GATE diagnostic software, Megagen, Daegu, Korea) was used to establish the treatment plan for implant placement. At the first stage of implant surgery, GBR for horizontal augmentation was performed with DBBM ($Bio-Oss^{(R)}$, Geistlich, Wolhusen, Switzerland), irradiated allogenic cancellous bone and marrow (ICB $cancellous^{(R)}$, Rocky Mountain Tissue Bank, Denver, USA), 3D-titanium membrane ($i-Gen^{(R)}$, Megagen, Daegu, Korea), resorbable collagen membrane (Collagen $membrane^{(R)}$, Genoss, Suwon, Korea), and A-PRF because there was approximately 4 mm labial dehiscence after implant placement. Five months after placing the implant, the second stage of implant surgery was performed, and healing abutment was connected after removal of the 3D-titanium membrane. Five months after the second stage of implant surgery was done, the final prosthesis was then delivered. Case 2. A 35-year-old female patient presented with discomfort due to pain and mobility of implant #21. Removal of implant #21 fixture was planned simultaneously with placement of the new implant fixture. At the first stage of implant surgery, GBR for horizontal augmentation was performed with DBBM ($Bio-Oss^{(R)}$), irradiated allogenic cancellous bone and marrow (ICB $cancellous^{(R)}$), 3D-titanium membrane ($i-Gen^{(R)}$), resorbable collagen membrane (Ossix $plus^{(R)}$, Datum, Telrad, Israel), and A-PRF because there was approximately 7 mm labial dehiscence after implant placement. At the second stage of implant surgery six months after implant placement, healing abutment was connected after removing the 3D-titanium membrane. Nine months after the second stage of implant surgery was done, the final prosthesis was then delivered. In these two clinical cases, wound healing of the operation sites was uneventful. All implants were clinically stable without inflammation or additional bone loss, and there was no discomfort to the patient. With the non-resorbable titanium membrane, the ability of bone formation in the space was stably maintained in three dimensions, and A-PRF might influence soft tissue healing. This limited study suggests that aesthetic results can be achieved with GBR using 3D-titanium membrane and A-PRF in the anterior maxilla. However, long-term follow-up evaluation should be performed.