• Korean Journal of Environmental Agriculture
• /
• v.36 no.4
• /
• pp.241-248
• /
• 2017

BACKGROUND: RNA interference (RNAi) eliminates or decreases gene expression by disrupting the target mRNA or by interfering with translation. Recently, RNAi technique was applied to generate new crop traits which provide protection against pests. To establish the environmental risk assessment protocol of RNAi LMO in lab scale, we developed dsRNA expression system using E. coli and tested acute oral toxicity assay to honey. METHOD AND RESULTS: The dsRNA expression vector, L4440, was chosen and cloned 240 bp of Snf7 and GFP gene fragment. To develop the maximum dsRNA induction condition in E. coli, we tested induction time, temperature and IPTG concentration in media. To estimate the risk assessment of dsRNA to honey bee, it has been selected and cultured with dsRNA supplement for 48 hours according to OECD guideline. As a result, the optimum condition of dsRNA induction was $37^{\circ}C$, 4 hours and 0.4 mM IPTG concentration and the difference between Snf7 and GFP dsRNA molecules from E. coli was not significant in survival and behavior to honey bee. Furthermore, blast search results indicated that effective match of predicted dsRNA fragments were not existed in honey bee genome. CONCLUSION: In this study, we developed and tested the acute oral toxicity of dsRNA using E. coli expression system to honey bee.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.36 no.4
• /
• pp.333-339
• /
• 2003

New manufacturing process was applied to manufacture the low salted Squid-Jeotgal to improve the quality variation salty taste and shelf-life. It's optimum salting and sugaring process was made by the addition of $17\%$ NaCl for 3 hr and $15\%$ corn syrup for 4 hr with 10 rpm agitation. Quality variations of Squid-Jeotgal produced by the improved and the conventional process were packed by jar, polyethylene lerephthalatefpolyethyleneilinear low density polyethylene (PET) and low density polyethylene (PE). And then quality variations of them were investigated at storage temperature of 10, 20, and $30^{\circ}C.$ Decreasing rate of pH, increasing rates of VBN and viable cell counts of Squid-Jeotgal produced by the improved process were slower than those of the conventional process at all storage temperature. Sensory evaluation indicated that the production of Squid-Jeotgal by the improved process extended the shelf-life about 10-20 days.

• Journal of Pharmaceutical Investigation
• /
• v.41 no.6
• /
• pp.347-354
• /
• 2011

Repeated oral administration of loxoprofen can induce many side effects such as gastric disturbances and acidosis. Therefore, we considered alternative routes of administration for loxoprofen to avoid such adverse effects. The aim of this study was to develop an ethylene-vinyl acetate (EVA) matrix system containing a permeation enhancer for enhanced transdermal delivery of loxoprofen. The EVA matrix containing loxoprofen was fabricated and the effects of drug concentration, temperature, enhancer and plasticizer on drug release were studied from the loxoprofen-EVA matrix. The solubility of loxoprofen was highest at 40% (v/v) PEG 400. The release rate of drug from drug-EVA matrix increased with increased loading dose and temperature. The release rate was proportional to the square root of loading dose. The activation energy (Ea), which was measured from the slope of log P versus 1000/T, was 5.67 kcal/mol for a 2.0% loaded drug dose from the EVA matrix. Among the plasticizer used, diethyl phthalate showed the highest release rate of loxoprofen. Among the enhancers used, polyoxyethylene 2-oleyl ether showed the greatest enhancing effect. In conclusion, for the enhanced controlled transdermal delivery of loxoprofen, the application of the EVA matrix containing plasticizer and penetration enhancer could be useful in the development of a controlled drug delivery system.

• Journal of fish pathology
• /
• v.24 no.1
• /
• pp.29-37
• /
• 2011

Effects of temperature ($13{\pm}1.5^{\circ}C$, $23{\pm}1.5^{\circ}C$) on the pharmacokinetic properties of nalidixic acid (NA) and piromidic acid (PA) were studied after oral administration to cultured black rockfish, Sebastes schlegeli. Serum concentrations of NA and PA were determined using HPLC-UV detector after a single dosage of 60 mg/kg body weight. At $23{\pm}1.5^{\circ}C$, the peak serum concentrations of NA and PA, which attained at 24 h post-dose, were 5.87 and $0.43\;{\mu}g/ml$, respectively. At $13{\pm}1.5^{\circ}C$, the peak serum concentrations of NA and PA, which attained at 10 h post-dose, were 6.22 and $1.57\;{\mu}g/ml$, respectively. Better absorption of PA was noted at $13{\pm}1.5^{\circ}C$ compared to $23{\pm}1.5^{\circ}C$. However, absorption of NA was not affected significantly by temperature. The elimination of NA and PA from serum of black rockfish was considerably faster at $23{\pm}1.5^{\circ}C$ than at $13{\pm}1.5^{\circ}C$. The kinetic profile of absorption, distribution and elimination of NA and PA in serum were analyzed by fitting to a one compartment model, with WinNonlin program. The AUC, $T_{1/2}$, $T_{max}$ and $C_{max}$, respectively, were: $161.25\;{\mu}g{\cdot}h/ml$, 0.15 h, 12.29 h and $8.91\;{\mu}g/ml$ at $23{\pm}1.5^{\circ}C$, and $134.12{\mu}g{\cdot}h/ml$, 0.18 h, 8.79 h and $5.00\;{\mu}g/ml$ at $13{\pm}1.5^{\circ}C$ with NA; $41.57\;{\mu}g{\cdot}h/ml$, 0.58 h, 8.24 h and $0.21\;{\mu}g/ml$ at $23{\pm}1.5^{\circ}C$, and $40.36\;{\mu}g{\cdot}h/ml$, 0.59 h, 5.04 h and $1.20\;{\mu}g/ml$ at $13{\pm}1.5^{\circ}C$ with PA.

• Journal of Dental Rehabilitation and Applied Science
• /
• v.25 no.1
• /
• pp.73-82
• /
• 2009

Injectable calcium phosphate cement (CPC) has been used as bone substitute successfully due to good biocompatibility and osteoconductivity. One of the important mechanical characteristics of CPC is flowablility, which can be evaluated by measuring rheological parameters. However, there have been few studies that measured rheological properties of CPC. The purpose of this study was to evaluate the effects of temperature and concentrations of 2 kinds of setting solutions, hydroxyprophyl methylcellulose (HPMC) and polyacrylic acid (PAA), on rheological properties of CPC. The CPC used was dicalcium phosphate dihydrate (DCPD). Rheological properties of CPC paste were measured using rheometer. The effect of concentrations of each solution (2% and 1% HPMC and 35% and 17.5% PAA) was evaluated. The effect of temperature ($25^{\circ}C$ and $37^{\circ}C$) on the rheological properties of CPC was also investigated. The statistical analysis was carried out with Mann-whitney test with Bonferronis collection. CPC with both setting solutions showed shear thinning behavior. Higher concentrations of setting solution (2% HPMC and 35% PAA) produced significantly higher viscosity than lower concentrations of setting solution (1% HPMC and 17.5% PAA). CPC with HPMC showed significantly higher viscosity at $37^{\circ}C$ that at $25^{\circ}C$. CPC with PAA showed lower viscosity at $37^{\circ}C$ than at $25^{\circ}C$, although the difference was not statistically significant. The results showed that CPC with HPMC or PAA solutions are pseudoplastic and the concentrations of setting solutions and temperature may have an effect on the rheological properties of CPC paste. These results showed that the flowability of injectable CPC could be improved by use of increasing frequency of oscillation. In clinical practice, the use of ultrasonic vibration would be helpful in application of injectable CPC. CPC with HPMC could be more easily applicated at $25^{\circ}C$ than $37^{\circ}C$. The use of lower concentrations of HPMC and PAA solution would be beneficial in terms of flowability.

• Sleep Medicine and Psychophysiology
• /
• v.1 no.1
• /
• pp.47-59
• /
• 1994

Objectives: The main purpose of this study was to investigate the possible differences in sleepiness, performance, and body temperature during the night between morning(M) and evening(E) type subjects. Methods: After a survey study, to verify the validity and reliability of the Korean translation of the Home' and $\ddot{O}stberg's$ Morningness-Eveningness Questionnaire(1976), 8 extreme M-type subjects(3males, 5 females) and 8 extreme E-type subjects(3 males, 5 females) were selected from the university student population who had participated in the survey study. All subjects underwent sleep latency test and a battery of performance tests at intervals of 2 hours through the night, from 23:00 to 07:00. Oral temperature of each subject was taken every hour from 21 : 00 to 8 : 00. Between the testing times, the experimenters ensured that subjects remained awake. Results: More profound sleepiness was found in the M-type compared to the E-type throughout the night, with significant differences in sleepiness occuring at 23:00 and 01:00 hours. Overall performance efficiency tended to be lower through, the night in the M-type than in the E-type on all tests. A difference in time of temperature minimum between the two types was not noteworthy. Rather, there appeared to be a substantial difference in temperature level during the declining phase, with the temperature of the M-type being lower than that of the E-type. Conclusions: These results indicate the existance of a temporal relationship between sleepiness, perfonnance and body temperature during night work. Since the M-type exhibited greater sleepiness and lower performance efficiency overnight than the E-type, it may be assumed that the E-type is more suitable for and tolerable to night work. There was some discussion of the limitations in generalizing these results together with some suggestions for future studies.

• The Journal of the Korean dental association
• /
• v.56 no.1
• /
• pp.58-65
• /
• 2018

Dental local anesthesia is important procedure for the elimination of pain during dental treatment. However, the pain during local anesthesia is one of the main source of fear to the patients. The cause of pain during dental local anesthesia includes soft tissue damage during penetration of the oral mucosa, pressure from the spread of the anesthetic solution, temperature of anesthetic solution, low pH of anesthetic solution, and the characteristics of the drug. Several concepts and devices introduced to date to reduce the pain during local anesthesia for dental treatment. In this report, devices that can reduce the pain during local anesthesia will be discussed.

• Journal of Korean Society of Industrial and Systems Engineering
• /
• v.23 no.57
• /
• pp.1-10
• /
• 2000

The purpose of this paper is to propose a method of rest to reduce work load of night shift workers for night shift work. The experiment was carried out 10minutes preparing time, 45minutes first work, 10minutes first rest, 45minutes second work, 10minutes second rest between 2 and 4 o'clock that the lowest physiological function of workers. The methods of rest set up as four patterns (1) non-action rest (2) non-action rest + listening music (3) action rest + non-action rest, (4) action rest + non-action rest + listening music. For the measurements of experiment, heart rates(R-R interval), critical flicker fusion frequency(CFF), blood pressure, oral temperature, reaction time and error rates were considered as criteria for work performance. As a result, action rest + non-action rest and action rest + non-action rest + listening music were more effective to reduce work load additional work than non-action rest and non-action rest + listening music.

• The Korean Journal of Pain
• /
• v.10 no.2
• /
• pp.274-277
• /
• 1997

The complex regional pain syndrome(CRPS) exhibit symptoms such as: abnormal skin color, temperature change, abnormal pseudomotor activity, edema. If CRPS is not treated appropriately at acute stage, then the affected extremity may become a useless, painful appendage. Treatment of CRPS by sympathetic blockade may be achieved by repeated intravenous regional guanethidine blocks, repeated anesthetic sympathetic blocks, surgical sympathectomy or oral sympatholytic therapy. We treated 29-year-old male patient with CRPS of left upper extremity by continuous cervical epidural blockade. Due to wound infection and dislocation of the epidural catheter, we inserted an implantable port system to inject the mixture of local anesthetics and small amount of morphine. After 10 months of treatment, patient was cured of symptoms and signs of CRPS and was able to resume a normal life.

• The Journal of the Korean dental association
• /
• v.15 no.12
• /
• pp.1027-1030
• /
• 1977

It has been widely believed that the exact measurement of the forces exerted on teeth and its structures by the lingual and perioral musculatures such as lip, cheek and tongue is important and significant in dentistry. Such measuring, moreover, is highly emphasized the importance of the fact that it can be of much help to study the physiological function displayed in the oral cavity. Recognizing the importance of measuring the pressures, the author has devised an electronic device consisting of pressure transducers utilizing reistance-strnain gauges. This electronic strain gauge was very easy to manipulate and its scale error was extremely minimized, unaffected by mouth temperature, mosture and external forces Author was able to read its results with attached meter without using calibration chart. Futhermore, the sensitivity of this electronic device was extremely high, Thus it facilitated to measure a force from 0 to 230 grams.