• 한국환경과학회지
• /
• 제26권11호
• /
• pp.1247-1253
• /
• 2017

The Fuji variety of apple, introduced in Japan, has excellent storage quality and good taste, such that it is the most commonly cultivated apple variety in Gunwi County, North Gyeongsang Province, Korean Peninsula. Accurate prediction of harvest maturity allows farmers to more efficiently manage their farm in important aspects such as working time, fruit storage, market shipment, and labor distribution. Temperature is one of the most important factors that determine plant growth, development, and yield. This paper reports on the beta distribution (function) model that can be used to simulate the the phenological response of plants to temperature. The beta function, commonly used as a skewed probability density in statistics, was introduced to estimate apple harvest maturity as a function of temperature in this study. The model parameters were daily maximum temperature, daily optimum temperature, and maximum growth rate. They were estimated from the input data of daily maximum and minimum temperature and apple harvest maturity. The difference in observed and predicted maturity day from 2009 to 2012, with optimal parameters, was from two days earlier to one day later.

• 한국수소및신에너지학회논문집
• /
• 제26권4호
• /
• pp.301-307
• /
• 2015

We present a study of hydrogen liquefaction using the CFD (Computational Fluid Dynamics) program. Liquid hydrogen has been evaluated as the best storage method because of high energy per unit mass than gas hydrogen, but efficient hydrogen liquefaction and storage are needed in order to apply actual industrial. In this study, we use the CFD program that apply navier-stokes equation. A hydrogen is cooled by heat transfer with the while passing gas hydrogen through Cu tube. We change diameter and flow rate and observe a change of the temperature and flow rate of gas hydrogen passing through Cu tube. As a result of, less flow rate and larger diameter are confirmed that liquefaction is more well. Ultimately, When we simulate the hydrogen liquefaction by using CFD program, and find optimum results, it is expected to contribute to the more effective and economical aspects such as time and cost.

• 한국수산과학회지
• /
• 제28권3호
• /
• pp.331-337
• /
• 1995

해조류 중 생산량이 많고 건강식품 소재로서의 가능성이 높은 미역과 다시마 해조묵을 제조하여 수침저장 중 저장 온도와 시간에 따른 생균수, pH, 적정산도, 겔강도 및 TVN의 변화를 측정하여 적정저장 조건을 검토한 결과 묵 저장 중 겔강도는 약화되었으며, TVN, 생균수는 증가하였다. 조체묵의 수침저장은 $32^{\circ}C$에서는 3일, $18^{\circ}C$ 이하에서는 15일 저장까지도 가능하였으며, 두유혼합묵은 조체묵 보다는 저장 기간이 짧아서 $18^{\circ}C$ 저장시 3일까지 가능한 것으로 판단되었다.

• 한국식품조리과학회지
• /
• 제23권1호통권97호
• /
• pp.50-61
• /
• 2007

The aim of this study was to determine the optimum amount of astringent persimmon concentrate to add to rice(polished rice, brown rice, and black rice) flour in the preparation of GamJeopyeon (persimmon rice cake). The moisture contents of polished rice, brown rice, and black rice persimmon Jeolpyeon were 50.37${\sim}$51.65%, 49.52${\sim}$52.44%, and 48.82${\sim}$51.87%, respectively. With increasing astringent persimmon content, the L value of all of the Jeolpyeon decreased, whereas the values of polished rice and brown rice persimmon Jeolpyeon increased. The b values of polished rice, brown rice, and black rice persimmon Jeolpyeon were the highest at addition levels of 8%, 4%, and 12%, respectively. In the mechanical textural evaluation, the hardness of polished rice and brown rice, persimmon Jeolpyeon decreased with increasing astringent persimmon concentrate content during storage time, and was the highest at the 0% addition level in black rice Jeolpyeon after 2 days storage. In the sensory evaluation, the overall acceptability of polished rice, brown rice, and black rice persimmon Jeolpyeon was higher at 4, 8, 12, and 16% compared to the 0% addition level. According to the above study. Jelopyeon made with the addition of up to 16% astringent persimmon concentrate to polished rice, brown rice, and black rice flour was found to be the best recipe in terms of overall acceptability and textural qualities.

• 한국포장학회지
• /
• 제9권1호
• /
• pp.33-54
• /
• 2003

The aim of this study was to develop a model that could be used in the design of modified atmosphere packaging (MAP) for peaches. Respiratory data at 5, 10, $20^{\circ}C$ for peaches were gathered and altered for create useful respiration model. Packaging materials were conventional low density polyethylene and polypropylene with anti-fog, and anti-fungi treatments, and thickness was $30{\mu}m$ and $50{\mu}m$ each. Permeability tests were performed to find their oxygen, carbon dioxide, water vapor transmission rate as increases in temperature. Test results were then converted to logarithm format for MAP modeling. The maximum rate of oxygen uptake increased with increasing temperature. Optimum gas composition in the package system for fruits were set according to literature and upper or lower limits of oxygen and dioxide established. To predict gas composition at certain storage time, weight of fruits, film thickness, film type, and other variables, respiration rate was studied at various storage conditions. The results of tests were used to calculate Cameron's model and converted to a cubic estimation equation. The validity of the model was tested experimentally by observing actual atmospheric changes inside packages. This result of study may be useful for designing dynamic gas exchange MAP systems for similar agricultural products.

• Journal of Microbiology and Biotechnology
• /
• 제31권1호
• /
• pp.163-170
• /
• 2021

Enzyme replacement therapy for lysosomal storage diseases usually requires recombinant enzymes containing mannose-6-phosphate (M6P) glycans for cellular uptake and lysosomal targeting. For the first time, a strategy is established here for the in vitro mannosyl-phosphorylation of high-mannose type N-glycans that utilizes a recombinant Mnn14 protein derived from Saccharomyces cerevisiae. Among a series of N-terminal- or C-terminal-deleted recombinant Mnn14 proteins expressed in Pichia pastoris, rMnn1477-935 with deletion of N-terminal 76 amino acids spanning the transmembrane domain (46 amino acids) and part of the stem region (30 amino acids), showed the highest level of mannosyl-phosphorylation activity. The optimum reaction conditions for rMnn1477-935 were determined through enzyme assays with a high-mannose type N-glycan (Man8GlcNAc2) as a substrate. In addition, rMnn1477-935 was shown to mannosyl-phosphorylate high-mannose type N-glycans (Man7-9GlcNAc2) on recombinant human lysosomal alpha-glucosidase (rhGAA) with remarkably high efficiency. Moreover, the majority of the resulting mannosyl-phosphorylated glycans were bis-form which can be converted to bis-phosphorylated M6P glycans having a superior lysosomal targeting capability. An in vitro N-glycan mannosyl-phosphorylation reaction using rMnn1477-935 will provide a flexible and straightforward method to increase the M6P glycan content for the generation of "Biobetter" therapeutic enzymes.

• 한국식품조리과학회지
• /
• 제14권1호
• /
• pp.1-8
• /
• 1998

부재료를 달리하여 제조한 김치의 발효속도특성 및 미생물학적 발효양상을 조사하였다. 발효 진행에 따라 김치 시료들의 pH는 감소하고 총산도는 증가하였고, 산화환원전위는 감소되다가 다시 증가하는 현상을 보였고 발효 최적기에 가장 낮은 값을 나타내었다. 또한 전 발효 기간 동안 생강, 고추가루 및 혼합시료에서 총생균수와 젖산균수가 많았으며, Leu. mensenteroides의 수는 생강과 고추가루 시료에 많았다. 김치의 적숙기에 젖산균 및 Leu. mensenteroides의 수가 최대이었고 Leu. mensenteroides가 감소하는 시기에 Lac. plantarum이 생기기 시작하였다. 이러한 발효속도 및 미생물 특성의 경시적 변화가 마늘 시료에서는 느리게 적생고추 시료에서는 빠르게 진행되었다. 따라서, 마늘은 젖산균 발효를 비교적 낮은 수준으로 오래 지속되게 하여 김치의 가식일수를 늘이는 저장성 향상에 필요한 부재료이고, 생강과 고추가루는 젖산균과 그 중 Leu. mensenteroides의 생육을 촉진하여김치 맛을 좋게하는데 도움이 되는 부재료라고 생각된다. 그리고, 산화환원전위가 김치의 발효 최적기를 찾는데 유용한 지표가 됨을 알 수 있었다.

• 농업과학연구
• /
• 제49권1호
• /
• pp.113-120
• /
• 2022

Leaf blight disease caused by Stemphylium species is an important disease in Allium crops, specifically onion, garlic and welsh onion. In 2018, leaf blight symptoms were severe and damaged onion and garlic in Jeonnam province in Korea. In addition, small purple spots on garlic burbs were observed in a post-harvest storage warehouse. Several Stemphylium isolates were isolated from diseased leaves from the field and from garlic bulb samples and were analyzed in terms of homology and the phylogenetic relationship based on the internal transcribed spacer region and calmodulin gene sequence. The results showed that among three Stemphylium species identified, S. vesicarium is most prevalent on onion and garlic. S. eturmiunum was for the first time identified as pathogenic to onion and garlic, whereas S. solani was found in welsh onion crops. Although these isolates grew well at the optimum temperature at 20 - 25℃, they could also grow at low temperatures of 10 - 15℃. A pathogenicity test was conducted using S. vesicarium and S. eturmiunum on onion and garlic respectively. These results showed that two Stemphylium species were highly virulent with cross pathogenicity in onion and garlic. The results of this study can support the biological characterization of Stemphylium species in Korea. Moreover, further research will need to develop fungicide application strategies for onion and garlic crops.

• 환경영향평가
• /
• 제21권6호
• /
• pp.823-839
• /
• 2012

Environmental Specimen Banks (ESBs) are playing pivotal role in monitoring the effect of environmental pollution on the ecosystem based on the retrospective analysis of the representative samples collected regularly and stored in cryogenic condition. In Korea, National Environmental Specimen Bank (NESB) was established in 2009 and the standard operating procedures (SOPs) for sampling, and cryogenic milling and storage had been prepared during 2007-2010. Since then, the tentative SOPs for the seven kinds of specimens (shoots of Red Pine (Pinus densiflora) and Korean Pine (Pinus koraiensis), leaves of Mongolian Oak (Quercus mongolica) and Zelkova Tree (Zelkova serrata), eggs of Feral Pigeon (Columba livia var. domestica), muscles and organs of Common Carp (Cyprinus carpio), and Freshwater Bivalve (Unio (Nodularia) douglasiae)) have been put to test in the field and laboratory as well against the practicality and feasibility. The SOPs were improved by reflecting the findings from the research and the following discussion regarding the selection of specimen (Feral Pigeon suffering from a control management), sample size (a problem of decreasing number of sampling trees related to increasing sampling time) and period (a problem related to a bud growth), and sampling methods etc.. In addition, barcoding system for the management of the specimen information, and monitoring system of the cryogenic storage to regulate the optimum temperature and the liquid nitrogen level were also developed for the efficient and effective control of the samples. Lastly, the safety guide and emergency protocol were augmented to guarantee a safe work environment with the cryogenic facility. These improvements of the SOPs are expected to contribute to more stable operation of the NESB.

• 한국식품과학회지
• /
• 제35권2호
• /
• pp.254-259
• /
• 2003

본 연구에서는 동결건조된 열무김치를 0, 10, $25^{\circ}C$에 60일간 저장하면서 동결건조/저장/복원시료의 젖산균수, pH, 관능적특성을 관찰하여 다음과 같은 결과를 얻었다. 동결건조된 열무김치의 적정한 복원시간은 2시간이었다. 동결건조/저장/복원시료의 저장중 젖산균수는 저장 10일만에 표준시료와 비교하여 유의적으로 감소하였으며(p<0.05), 그 이후에는 큰 변화가 없었다. 한편 저장온도가 높을 수록 10일시료의 젖산균수 감소 정도가 높았다. pH는 동결건조에 의하여 다소 증가하였으나, 60일 저장기간 중에 점차 저하하는 경향을 보였다. 동결건조/복원 열무김치의 관능적특성은 표준시료(동결건조전 시료)와 비교하면 기호성이 다소 낮았으나, 동결건조/복원시료만 단독으로 시식하면 기호성이 비교적 양호하였다. 동결건조/저장/복원시료의 전반적인기호도는 $0^{\circ}C$저장시료의 경우 표준시료보다 유의적으로 낮았으며(p<0.05), 저장온도가 증가할수록 전반적인기호도도 점차로 저하하였다. 저장기간이 10일에서 60일로 연장됨에 따라 $0^{\circ}C$나 $10^{\circ}C$저장시료의 관능적 특성은 다소 저하하였으나 $25^{\circ}C$저장시료의 관능적특성은 현저하게 저하하였다.