• Microbiology and Biotechnology Letters
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• v.23 no.3
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• pp.275-281
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• 1995

From the plasmid pYA300 carring a CMCase of Rhizobium fredii USDA193 plasmid was subcloned into pBluescript II KS(+)/pBluescript II SK(+) vectors and designated pYA500 and pYA600, respectively. Escherchia coli cells transformed with pYA500 porduced the CMCase more than with pYA600. The orientation of the cloned fragment in pBluescript vector had the effect on gene expression in E. coli background. When the 1.7 kb CMCase gene fragment of R. fredii USDA193 was hybridized to EcoRI-digested total DNA from R. meliloti and R. fredii USDA 191 the unique bands hybridized respectively, indicating that some genetic diversity exists in the EcoRI restriction enzyme site for CMCase gene in Rhizobium strains. The optimum pH of enzyme activity was 7 and the optimum temperature of that was nearly 37$\circ$C. The cellulase-minus derivatives of pYA500 were constructed by Tn5 insertional mutation. Among 6000 transconjugants, two mutant plasmids (designated pYA500::Tn5a and pYA500::Tn5b) were detected from the cellulase- negative transconjugants. The product of CMCase gene was analyzed by one dimensional SDS- PAGE of the cell extracts. About 45 kDa protein was considered to be a product of CMCase gene.

• KCID journal
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• v.18 no.1
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• pp.4-17
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• 2011

The standard design methodology was suggested to construct wetland system for reducing non-point source pollution from Saemangeum reclaimed paddy land. To set for the design flow and concentrations, runoff and water quality survey were conducted during the irrigation period in 2008 at Gyehwa reclaimed paddy land located at near Saemangeum lake. It is rational that 1ha is the optimum constructed wetland size. To meet this size, the moderate drainage area of reclaimed paddy field was 50ha under the conditions that rainfall is 30mm, average runoff coefficient is 0.83, and runoff capture ratio is 0.6. At these condition, the runoff volume from 50ha was 10,520 $m^3/d$ including base flow during irrigation period. To select the optimum wetland system, several case studies were conducted by focusing on the tidal reclaimed land areas having wetland systems in Seokmun. Pond-Wetland system was selected as the standard model because of showing the highest reduction efficiency. Single variable regression equation were delivered to estimate effluent water concentrations from the designed wetland by using long-term monitoring data from the Seokmun experiment site. The effluent concentration from the designed wetland using these equation were showed moderately range.

• Korean Journal of Materials Research
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• v.33 no.4
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• pp.151-158
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• 2023

In this study, a new type of composite material combined with carbonyl iron, a relatively strong ferromagnetic material, was prepared to overcome the current application limitations of Prussian blue, which is effective in removing radioactive cesium. The surface of the prepared composite was analyzed using SEM and XRD, and it was confirmed that nano-sized Prussian Blue was synthesized on the particle surface. In order to evaluate the cesium removal ability, 0.2 g of the composite prepared for raw cesium aquatic solution at a concentration of 5 ㎍ was added and reacted, resulting in a cesium removal rate of 99.5 %. The complex follows Langmuir's adsorption model and has a maximum adsorption amount (q_e) of 79.3 mg/g. The Central Composite Design (CCD) of the Response Surface Method (RSM) was used to derive the optimal application conditions of the prepared composite. The optimal application conditions achieved using Response optimization appeared at a stirring speed of pH 7, 17.6 RPM. The composite manufactured through this research is a material that overcomes the Prussian Blue limit in powder form and is considered to be excellent economically and environmentally when applied to a cesium removal site.

• Journal of Korea Water Resources Association
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• v.40 no.12
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• pp.995-1005
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• 2007

This study presents a framework for optimum scale determination for small hydropower development in a river basin. The framework includes the construction of hydrology and topography data, the simulation of hydropower operation, the economic analysis, and the determination of optimum scale of the small hydropower. The optimum scale of design flow and facility are determined by Net Present Value among economic analysis indices. The investment cost is estimated by the cost function derived from the construction cost of existing small hydropower plants. The benefit from power generation is estimated by the price announced by government. The presented framework is applied to the two potential sites in Cho River basin for the dam and run-of-river type of plants. Finally, the sensitivity analysis for a design flow and scale of the plant is performed for the each site. The usage of the framework presented in the study is highly expected for the estimation of potential hydropower resources or the decision support tool for a proprietor by estimating the optimum scale and economical feasibility in advance.

• The Journal of Engineering Geology
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• v.12 no.1
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• pp.35-51
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• 2002

This study aims to establish the methodology for design of an optimum water curtain system of the unlined underground oil storage cavern satisfying the requirements of hydrodynamic performance in a volcanic terrain of the south coastal area. For the optimum water curtain system in the storage facility, the general characteristics of groundwater flow system in the site are quantitatively described, i.e. distribution of hydraulic gradients, groundwater inflow rate into the storage caverns, and hydrogeologic influence area of the cavern. In this study, numerical models such as MODFLOW, FracMan/MAFIC and CONNECTFLOW are used for calculating the hydrogeological stability parameters. The design of a horizontal water curtain system requires considering the distance between water curtain and storage cavern, spacing of the water curtain boreholes, and injection pressure. From the numerical simulations at different scales, the optimum water curtain systems satisfying the containment criteria are obtained. The inflow rates into storage caverns estimated by a continuum model ranged from about 120 m$^3$/day during the operation stage to 130~140m$^3$/day during the construction stage, whereas the inflow rates by a fracture network model are 80~175m$^3$/day. The excavation works in the site will generate the excessive decline of groundwater level in a main fracture zone adjacent to the cavern. Therefore, the vertical water curtain system is necessary for sustaining the safe groundwater level in the fracture zone.

• Korean Journal of Medicinal Crop Science
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• v.27 no.5
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• pp.322-329
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• 2019

Background: Studies have suggested that the northern provinces of Gangwon-do are good sites for licorice (Glycyrrhiza uralensis Fisch.) cultivation in Korea, as they have similar temperatures to its original locations in northern China. However, poor growth and freezing injury are often reported in Korea. Therefore, it is necessary to reassess the domestic cultivation site of licorice. Methods and Results: To determine the optimum temperature for cultivating licorice, the growth, yield, and pharmacological characteristics of G. uralensis were assessed in a temperature gradient tunnel at Eumseong, Chungcheongbuk-do, Korea in 2017. Plant height increased until the temperature rose to $5.9^{\circ}C$ above the local external temperature. Yield (㎏/10a) increased by 46.9% when the growing temperature was $1.5^{\circ}C$ to $3.0^{\circ}C$ (T2) above the external temperature and by 72.6% when the growing temperature was $3.0^{\circ}C-4.5^{\circ}C$ (T3) above the external temperature. However, a difference of $4.5^{\circ}C-5.9^{\circ}C$ (T4) above the external temperature, decreased the yield by 9.8% compared to that at T2. The glycyrrhizin content of G. uralensis roots in each temperature band was 0.72%, 0.53%, 0.91%, and 0.84% (T1, T2, T3, T4), these differences appear to result form individual plant variation rather than growth temperature. Conclusions: Based on the results of this study, we estimate that the temperature-based optimum cultivation site for G. uralensis in Korea is the south central region, rather than the northern province of Gangwon-do. Improvement in growth and yield maybe observed if the plantations in the central Jecheon (Chungcheongbuk-do, Korea) are expanded into the south central region.

• Journal of Korean Society of Transportation
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• v.19 no.5
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• pp.99-108
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• 2001

A framework of using and integrating GIS and OR tools for determining the best site selection has been provided. In this research, we demonstrated that both the P-Median heuristic method and MIP method can be successfully applied to the optimum site selection problem of oil tank location selection. Furthermore, the results identified by both approaches are identical. To accomplish this, both GIS road and maritime networks have been constructed and combined to calculated the minimum distance matrix, which is required by both approaches. After the application to the Korean peninsula, the facility locations chosen are Kunsan, Yosu, Busan, and Okgye for each district. As has been shown, the power of GIS and both algorithm have been demonstrated throughout the research and further similar research can also be conducted using the power of GIS and Operations Research tools.

• Journal of Microbiology and Biotechnology
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• v.9 no.1
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• pp.62-69
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• 1999

In order to investigate the critical amino acid residues involved in the catalytic activities of $\beta$-cyclodextrin glucanotransferase ($\beta$-CGTase) excreted by Bacillus firmus var. alkalophilus, the amino acid residues in $\beta$-CGTase were modified by various site-specific amino acid modifying reagents. The cyclizing and amylolytic activities of $\beta$-CGTase were all seriously reduced after treatment with Woodward's reagent K (WRK) modifying aspartic/glutamic acid, N-bromosuccinimde (NBS) modifying tryptophan, and diethylpyrocarbonate (DEPC) modifying histidine residues. The roles of tryptophan and histidine residues in $\beta$-CGTase were further investigated by measuring the protection effect of various substrates during chemical modification, comparing protein mobility in native and affinity polyacrylamide gel electrophoresis containing soluble starch, and comparing the $K_m$ and $V_{max}$ values of native and modified enzymes. Tryptophan residues were identified as affecting substrate-binding ability rather than influencing catalytic activities. On the other hand, histidine residues influenced catalytic ability rather than substrate-binding ability, plus histidine modification had an effect on shifting the optimum pH and pH stability.

• Environmental Engineering Research
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• v.20 no.1
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• pp.41-50
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• 2015

The cyanobacterium Anabaena doliolum Ind1 isolated from a coal mining site was tested for removal of cadmium at optimum pH 7.0 and temperature $25^{\circ}C$. The organism recorded high percentage of metal removal (92-69%) within seven days of exposure to 0.5-2.0 ppm cadmium. Biosorption onto the cell surface was the primary mode of metal removal. Fourier transform infrared spectroscopy (FTIR) established hydroxyl, amides, carboxyl, sulphate and carbonyl groups to be the major functional groups on the cell surface involved in cadmium binding. Cellular ultrastructure and a range of vital physiological processes (i.e., photosynthetic pigments, respiration, photosynthesis, heterocyst frequency and nitrogenase activity) remained unaffected upon 0.5 ppm treatment; higher concentrations of cadmium exerted visible adverse effects. Amongst the five photosynthetic pigments tested, phycocyanin was the most targeted pigment (inhibition was 15-89%). Both respiration and photosynthetic activities were inhibited by cadmium with more severe effect seen on respiration. 2.0 ppm cadmium exposure also had drastic negative effect on nitrogenase activity (87% decreased).

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.22 no.6
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• pp.404-412
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• 2010

The purpose of this study is to accomplished an in-site evaluation method for existing building insulation status using Infra-red camera and to consider improvement performance to prevent condensation and draw the optimum insulation design method for building using simulation tool. The research contents of this study are to evaluate validity and suitability of building insulation defect survey using Infra-red camera for apartment housing with temperature and heat flow pattern analyze method. Based on this research, the three corners, weak part in condensation, were selected in apartment building and conducted simulation by three-dimensional steady state. From the results, it is required to strengthen insulation design, and it is founded that existing insulation system typically applied to most Korean apartment housings have serious insulation defect that insulation is disconnected by structural components at the joints of wall-slab and wall-wall in envelope. Thus, it is considerate to need a concrete technology improvement.