• 한국식품저장유통학회지
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• 제10권3호
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• pp.417-420
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• 2003

해양 심층수 첨가에 따른 효모 발효력과 심층수 첨가 최적 농도를 알아보기 위하여 효모균주 9종에 대하여 증식을 조사하였다. 효모균주는 Saccharomyces cerevisiae 10호, 11호, 12호, 901, RCY, Sacch.cerwisiae ktwef DJ97, Saccharomyces cerevisiae YJK, JK99, CMY-28 등이었으며, 해양 심층수는 경도 250, 500, 1000으로 조절하였고 대조군과 함께 증식력을 측정하였다 Saccharomyces cerevisiae 12호 균주가 심층수 경도 500인 시험군에서 증식력도 높았으며, Sacch. cerevisiae 901에서는 경도 1000인 시험군이 대조군에 비해 증식력이 높게 나타나 효모균주의 종류와 심층수의 첨가량에 따라 적응력이 강한 균주가 있었다. 당농도와 심층수 첨가량에 따른 Sacch. cerevisiae kluyevery DJ97의 발효력은 심층수 경도 200의 당 10％ 첨가군에서 가장 잘 증식하였다.

• 한국잠사곤충학회지
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• 제36권2호
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• pp.130-137
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• 1994

천잠의 사육환경을 구명하기 위하여 사료자원, 기상환경 및 사육방법등에 관하여 조사한 결과를 다음과 같이 요약한다. 1. 천잠의 실내사육에 있어 사육온도는 항온보다 변온이 좋은 편이며, 치잠기 25~26$^{\circ}C$, 장잠기 22~23$^{\circ}C$ 범위가, 사육습도는 치잠기 80~90%, 장잠기 60~70% 범위가 적당한 것으로 나타났다. 2. 천잠사육에 있어 광조기는 유충의 성장에 미치는 영향이 크지 아니하였다. 3. 귀잠시기는 빠른것이 늦은 것보다 유충시기에 단축되는 경향이 있으며 질도 양호한 것으로 나타났다. 4. 사료수종은 상수리나무가 밤나무보다 더 적합한 것으로 나타났다. 야생수보다 재배수가 사육성육이 더 좋았다. 5. 천잠을 옥외사육으로 전환하는 시기는 빠를수럭 작황이 불안정해 지는 경향을 보였다.

• 한국세라믹학회지
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• 제27권5호
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• pp.597-604
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• 1990

In Cubic ZrO2 crystal growth by Skull method, it was examined on effect of homonization on melt as keeping ZrO2(90mol%)-Y2O3(10mol%) melt with 1hr, 2hr, 4hrs, 8hrs, 16hrs, respectively. The optimum homonizing condition in this system was obtained by the examination between quality of grown crystals and soaking time of melt. It was obtained that the lower quality crystal could be produced in the longer holding melt than the optimum soaking time of melt in spite of the supposedly well homonized state, because the melt stability is sensibly dependent on the convective state of melt in skull method (cold crucible process).

• 한국식품과학회지
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• 제13권2호
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• pp.91-100
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• 1981

두부폐수를 이용(利用)한 SCP생산(生産)의 기초 연구로서 유용(有用)효모의 검색(檢索), 최적배지선정 및 배양조건이 검토(檢討)되었다. 총 18종(種)의 효모중(中) 두부폐수를 가장 잘 이용(利用)하는 효모 C.utilis YUFE 1508과 C. guilliermondii KFCC 35120을 선정했으며 이들 효모의 최적생육(生育) pH는 $6.0{\sim}6.5$였고 최적배양온도는 $25^{\circ}C$였다. 탄소원으로서는 molasses 2.5%(전당기준), 질소원으로 ammonium acetate $0.1{\sim}0.3%$ (N함량기준), P 및 K원으로 $K_2HPO_4\;0.1{\sim}0.2%$ (P함량기준) 및 $K_2HPO_4$ 0.05% (P함량기준)를 두부폐수에 각각(各各) 첨가(添加) 함이 효모 생육(生育)에 가장 효과적(效果的)이었으며 금속염(金屬鹽)의 첨가는 생육(生育)에 아무런 영향을 미치지 못했다. 최적배양조건에서 48시간 배양했을때 최대(最大) 생육(生育)에 도달했으며 이때의 배양액 100ml의 건조균체중량은 C. utilis YUFE 1508이 1.313g, C. guilliermondii KFCC 35120의 경우가 1.322g이었다. 이를 대당(對糖)건조균 체수율로 환산하면 각각 68.4%, 74.2%였다. 또한 건조효모균체의 조단백질 함량은 C. utilis YUFE1508 이 약 54.0%, C. guilliermondii KFCC 35120의 경우가 56.8%정도였다.

• 한국버섯학회지
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• 제1권1호
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• pp.21-27
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• 2003

국내외에서 수집된 큰양송이(포토벨라)의 균주와 양송이 ASI707을 공시하여 균사체 배양조건과 균주별 자실체의 형태적 특성을 조사한 결과는 다음과 같다. 퇴비추출배지에서 균사생장이 우수하였고, 최적배양온도는 $25^{\circ}C$, pH는 7.0 이었다. 균사생장에 가장 적합한 탄소원으로 Sucrose와 Starch 질소원으로는 Malt extract가 선발되었고 최적 C/N율은 10이었다. 인공재배한 자실체는 공시균주간에 갓크기, 갓두께 등에서 큰 차이가 없었으나 개체중은 25.8~33.8g 내외로 균주간에 다소 차이를 보였다. 공시균주 중 KME59007은 다발성으며 평당 수량도 44.6kg으로 가장 많았다.

• 수산해양교육연구
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• 제26권4호
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• pp.789-796
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• 2014

This study was conducted to investigate the effects of feeding rate on growth performance, blood components and histology of growing olive flounder. The experiment was carried out to determine the optimum feeding rate of the fish (initial fish mean weight of $97{\pm}3.0$ g) at the optimum water temperature. Two replicated groups of fish were fed commercial diet at the feeding rates of 0, 1.0, 1.65 and 2.3% body weight (BW)/day and satiation. Feeding trial was conducted under a flow-through system with ten 1.2 metric ton aquaria receiving filtered seawater at $21{\pm}2^{\circ}C$ for three weeks. Weight gain and specific growth rate (SGR) for fish fed to satiation were significantly higher than those for the unfed fish and for fish fed at 1.0 and 1.65% BW/day. There were no significant differences in values of such parameters between fish fed at 1.65% and those fed at 2.3% BW/day and between fish fed at 2.3% and those fed to satiation. Weight gain and SGR for the unfed fish were significantly lower than those for fish in the other treatments. All the tissues (hepatopancreas, kidney and anterior intestine) were in good condition in fish fed the experimental diet at different feeding rates. This result indicates that the optimum feeding rates of olive flounder (97-160 g) was approximately 2.52% BW/day at the optimum water temperature.

• 한국세라믹학회지
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• 제31권12호
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• pp.1423-1428
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• 1994

TiO2 doped sapphire single crystals were grown by Verneuil method. The doping amount of TiO2 to Al2O3 were varied 0.1, 0.2, 0.3 wt% respectively. The grown crystals have reddish color and somewhat transparent. Optimum growth condition was established by changing growth rate and gas flow ratio. Growth condition are as follows; The flow rate range of oxygen ws 5.0~7.3 ι/min and that of hydrogen was 16~25ι/min and average growth rate was 6~8mm/hr. The basic cause of color appearence and defects in crystal were studied.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2018년도 춘계학술발표회
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• pp.36-36
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• 2018

Soybean is the important crop in Asian countries as protein source, oil production and animal feed. Improving soybean using genetic transformation is the principal tool in nowadays. Developing herbicide resistant transgenic soybean plants through Agrobacterium-mediated transformation has been worked in many previous studied. However, the transformation efficiency is still low. Many attempts try to find the optimum media condition for plant regeneration after infection. After transformation, the plant regeneration is very important condition to promote growth of transgenic plant. In this study, we optimized a regeneration condition for two Korean soybean cultivar, Dawonkong and Pungsannamulkong using cotyledon, cotyledonary nodes and hypocotyl as explant. The results showed that shoot regeneration of cotyledonary nodes on B5 medium containing 2 mg/L 6-benzylaminopurine showed the highest percentage of regeneration in Dawonkong (75.8%) while Pungsannamulkong presented high number of shoots 2.12 shoots per explant. For transformation condition, co-cultivation in 7 days showed a high number of GUS positive expression. Most of explants can survived under media including 5 mg/L of glufocinate which refers phosphinotricin for 2-week selection. Washing with 400 mg/L of cefotaxime in several times and selection in plant regeneration media with 400 mg/L of cefotaxime can prevent bacteria growth, effectively.

• Journal of Microbiology and Biotechnology
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• 제33권2호
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• pp.228-234
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• 2023

In this study, the performance of a gold biosensor combined with light microscope imaging system (GB-LMIS) was comparatively evaluated against enzyme-linked immunosorbent assay (ELISA) for detecting Salmonella under simulated chilling condition. The optimum concentration of antiSalmonella polyclonal antibodies (pAbs) was determined to be 12.5 and 100 ㎍/ml for ELISA and GBLMIS, respectively. GB-LMIS exhibited a sufficient and competitive specificity toward three tested Salmonella among only. To mimic a real-world situation, chicken was inoculated with Salmonella cocktail and stored under chilling condition for 48 h. The overall growth of Salmonella under chilling condition was significantly lower than that under non-exposure to the chilling condition (p < 0.05). No significant differences in bacterial growth were observed between brain heart infusion and brilliant green broth during the enrichment period (p > 0.05). Finally, both GB-LMIS and ELISA were employed to detect Salmonella at every 2-h interval. GB-LMIS detected Salmonella with a competitive specificity by the direct observation of bacteria on the sensor using a charge-coupled device camera within a detection time of ~2.5 h. GB-LMIS is a feasible, novel, and rapid method for detecting Salmonella in poultry facilities.

• 한국전기전자재료학회논문지
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• 제23권11호
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• pp.848-853
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• 2010

We have grown GaN nanowires by the low pressure MOCVD method on Ni deposited oxidized Si surface and have established optimum conditions by observing surface microstructure and its photoluminescence. Optimum growth temperature of $880^{\circ}C$, growth time of 30 min, TMG source flow rate of 10 sccm have resulted in dense nanowires on the surface, however further increase of growth time or TMG flow rate has not increased the length of nanowire but has formed nanocrystals. On the contrary, the increase of ammonia flow has increased the length of nanowires and the coverage of nanowire over the surface. The shape of nanowire is needle-like with a Ni droplet at its tip; the length is tens of micron with more than 40 nm in diameter. Low temperature photoluminescence obtained from the sample at optimum growth condition has revealed several peaks related to exciton decay near band-edge, but does not show any characteristic originated from one dimensional quantum confinement. Strong and broad luminescence at 2.2 eV is observed from dense nanowire samples and this suggests that the broad band is related to e-h recombination at the surface state in a nanowire. The current result is implemented to the nanowire device fabrication by nanowire bridging between micro-patterned neighboring Ni catalysis islands.