• 제목/요약/키워드: optimum dosage

검색결과 274건 처리시간 0.023초

Aspergillus niger CF-34 효소를 이용한 두부 또는 두유비지의 가용화 (Solubilization of Tofu-Residue Using Multienzyme Derived from Aspergillus niger CF-34)

  • 김강성;박은하;최연배;김교창;이상화;손헌수
    • 한국식품과학회지
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    • 제26권5호
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    • pp.484-489
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    • 1994
  • 두유 및 두부제조시 부생되는 비지를 효과적으로 이용하기 위한 방안으로 Aspergillus niger CF-34로부터 얻은 효소를 비지에 처리하여 가용화를 시도하였다. 비지가용화를 위한 본 연구에서 비지에 작용하는 복합효소액의 최적반응조건을 조사하였으며, 복합효소액 처리시 두유비지내 불용성 단백질의 회수율 및 고형분 용해도와 비지의 효소가수분해 특성을 검토하였고, 그 결과는 다음과 같다. 복합효소액의 사용량은 비지 고형분의 약 50%를 가용화하는 2.5%(% of total solid)를 최적 복합효소액의 농도로 선정하였다. 효소작용시간에 따른 비지의 단백질 회수율 및 고형분의 용해도는 효소반응후 3시간에서 각각 약 62%, 50%로 상대적으로 높은 값을 나타내었다. 비지를 alkali 처리(0.1% NaOH)로 입자구조를 변형시킴으로서 효소가수분해율이 증가되었고, 가수분해 시간도 단축됨에 따라 비지 가수분해의 효과적인 전처리방법임을 확인할 수 있었다.

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담수조류의 대량번식에 따른 피해를 최소화하기 위한 녹조제거기 개발 (The development of algae removal system to minimize the damage of algae bloom on freshwater)

  • 한재호;박우식;김종현;이영식;노준혁;김연규;윤범상
    • 한국해양환경ㆍ에너지학회지
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    • 제3권1호
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    • pp.62-69
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    • 2000
  • 본 연구는 담수조류의 대량 번식에 따른 정수장의 여과막 박힘, 어류의 대량폐사 등 각종 피해를 최소화하기 위하여 응집제를 이용한 응집-여과공정을 녹조제거시스템에 적용하기 위한 것이다. 응집-여과 공정에서 최적의 응집상태를 결정하기 위하여 시료로는 낙동강 원수를 사용하였고 Jar test와 실험실용 반응기를 사용하여 알칼리도, 탁도, Chl-a, pH를 측정하였다. 응집시간, 응집제 주입량, 드럼필터 회전속도 그리고 Chl-a는 각각 5min, 5mg/l, 3rpm 그리고 90㎍/l의 조건에서 높은 조류제거율을 보였다. Alum을 사용하였을 때의 조류 및 탁도 평균제거율은 50~60%, 30~50%이었고, PAC는 Chl-a의 제거율이 Alum보다 약 20% 더 좋은 효율을 보였다.

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소성굴패각분말과 황토의 동시 사용에 의한 적조생물의 응집 (Flocculation of Red Tide Organisms in Sea Water by Using an Ignited Oyster Shell Powder and Loess Combination)

  • 김성재
    • 한국수산과학회지
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    • 제36권6호
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    • pp.716-722
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    • 2003
  • This study determined the optimum dosage for coagulation reactions of red tide organisms (RTO) using a combination of ignited oyster shell powder (10sp) and loess and examined the electrokinetic and rheological characteristics of their flocs. Two kinds of RTO, Cylindrotheca closterium and Skeletonema costatum, were sampled in Masan Bay and cultured in the laboratory. Coagulation experiments were conducted using various concentrations of IOSP, loess, IOSP+1oess, RTO, and a jar tester RTO cell numbers were counted for both the supernatant and RTO culture solution. The removal rates increased rapidly with increasing IOSP concentrations up to 50 mg/L and loess concentrations up to 800 mg/L. A removal rate of $100\%$ was reached at 400 mg/L of IOSP and 6,400 mg/L of loess. The highest increment $(16.7\%)$ of the rates of coagulation reaction occurred using both IOSP and loess (50+200 mg/L) in comparison with IOSP alone. The rate of coagulation reaction using both IOSP and loess (50+200 mg/L), $90.6\%,$ was similar to employing either IOSP of 150 mg/L or loess of 3,200 mg/L. All of the coagulation liquids for RTO, IOSP (200 mg/L), loess (200 ma/L), and IOSP+1oess (200+200 mg/L) revealed non-Newtonian fluid properties and therefore their shear rate vs. shear stress curves were non-linear. The coagulation liquids revealed elastic body properties at a lower shear rate increasing in the following order: RTO, IOSP (200 mg/L), loess (200 mg/L), and IOSP+1oess (200+200 mg/L. IOSP+1oess (200+200 mg/L) especially demonstrated plastic flow properties at a lower shear rate.

국소치료용 구강점막패취의 제제설계 및 약제학적 특성 (Formulation and Pharmaceutical Properties of Local Mucoadhesive-Patch Preparation)

  • 이계주;서현주;이덕근;박종범;신광현;황성주
    • 약학회지
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    • 제42권2호
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    • pp.187-195
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    • 1998
  • In order to ameliorate disadvantages of buccal ointments and mucoadhesive tablets used for the treatment of aphthosis, a thin mucoadhesive patch containing triamcinolone acetoni de was designed and evaluated for the pharmaceutical properties. The adhesive gel layer consisting of Noveon AA-1, hydroxypropylcellulose-M and ethylcellulose N 100, and the protective gel layer of ethylcellulose N 100, Eudragit RSPO and castor oil have been formulated and various properties such as viscosity of drug gel layer, thickness, in vitro adhesion time, adhesive strength, surface pH, content uniformity and drug release are tested. The mean viscosity of drug-containing gel layer was found to increase with increasing amount of Noveon OAA-1 or hydroxypropylcellulose-M. The optimum formulation showed the thickness of 171 ${\mu}$m, surface pH of 4.6, in vitro adhesion time of 8 hours and adhesive strength of 272.7g/sheet. The drug content of each patch was relatively homogeneous with the value of 273${\pm}$6.77g. Drug release study showed that compared to mucoadhesive tablet, the patch showed a faster drug release. Drug release was delayed by hydroxypropylcellulose-M, but not by ethylcellulose N 100. The patches prepared were nonirritant and the muco adhesion was better than the commercial product (AftachR) on the market. Based on these results, this mucoadhesive patch is expected to be an effective dosage form for the treatment of aphthosis.

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만니톨의 효소 가수분해 반응 조건 최적화 (Optimization for Enzymatic Hydrolysis of Mannitol)

  • 박은영;김용진;정승미;이동훈
    • KSBB Journal
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    • 제28권2호
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    • pp.65-73
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    • 2013
  • This study aimed to investigate the enzymatic hydrolysis of mannitol using Viscozyme$^{(R)}$ L, Celluclast$^{(R)}$ 1.5 L, Saczyme$^{(R)}$, Novozym$^{(R)}$, Fungamyl$^{(R)}$ 800 L, Driselase$^{(R)}$ Basidiomycetes sp., and Alginate Lyase, and to optimize of reaction conditions for production of reducing sugar. Response surface methodology (RSM) based on central composite rotatable design was used to study effects of the independent variables such as enzyme (1-9% v/w), reaction time (10-30 h), pH (3.0-7.0) and reaction temperature ($30-70^{\circ}C$) on production of reducing sugar from mannitol. The coefficient of determination ($R^2$) of $Y_1$ (yield of reducing sugar by Viscozyme$^{(R)}$ L) and $Y_3$ (yield of reducing sugar by Saczyme$^{(R)}$) for the dependent variable regression equation was analyzed as 0.985 and 0.814. And the p-value of $Y_1$ and $Y_3$ showing 0.000 and 0.001 within 1% (p < 0.01), respectively, was very significant. The optimum conditions for production of reducing sugar with Viscozyme$^{(R)}$ L were 9.0 % (v/w) amount of enzyme, 30.0 hours of reaction time, pH 4.5 and $30.0^{\circ}C$ of reaction temperature, and those with Saczyme$^{(R)}$ were 9.0% (v/w) of amount of enzyme dosage, 30.0 h of reaction time, pH 7.0 and $30.0^{\circ}C$ of reaction temperature, consequently, the predicted reducing sugar yields were 22.5 and 27.9 mg/g-mannitol, respectively.

폴리카르본산계 고분자가 시멘트계 재료의 물성에 미치는 영향 (Effect of the Polycarboxylates on the Physical Properties of Cement Materials)

  • 조헌영;서정목;전기석;이기환;김진만
    • 콘크리트학회논문집
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    • 제13권4호
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    • pp.354-361
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    • 2001
  • 폴리카르본산계 고분자는 분산성이 양호하고 시멘트계 재료에서 분산 유지성이 뛰어나므로 최근 콘크리트용 혼화제로 크게 주목을 끌고 있다. 따라서 본 연구에서는 시멘트콘크리트용 분산제로서 수평균 분자량(Mn)이 적당한 poly(acrylate-co-methyla-crylate)를 합성하여 시멘트 모르타르의 분산성과 기계적 물성에 미치는 영향에 대하여 연구하였다. 폴리카르본산계 고분자의 수평균 분자량이 2,000~3,000 보다 5,000 정도로 비교적 큰 polycarboxylate가 시멘트계 재료의 물성을 크게 향상시키며, 최적 첨가량은 시멘트 중량의 0.6% 이다. 그러나 poly(acrylate-co-methylacrylate)계 고분자는 시멘트 모르타르 반죽에서 흐름도의 경시변화가 큰 것으로 나타났으며, 이러한 현상은 폴리카르본산계 서방성 고분자가 시멘트계 재료에 사용되었을 때, 알칼리성 분위기에서 카르복실기의 서방성에 의해서 분산성이 지속적으로 유지된다는 기존의 이론과 다르다.

마그네사이트 광석(鑛石)의 염산용해(鹽酸熔解) 특성(特性) 및 불순물(不純物) 제거 (Dissolution Characteristics of Magnesite Ore in Hydrochloric Acid Solution and Removal of Impurity)

  • 엄형춘;박형규;김철주;김성돈;윤호성
    • 자원리싸이클링
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    • 제18권6호
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    • pp.38-45
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    • 2009
  • 마그네사이트 광석의 염산용해 특성 및 불순물 제거에 대한 연구를 수행하였다. 마그네사이트의 용해율은 용해온도가 증가할수록, 광석 입도가 감소할수록 증가하였다. 최적 용해조건은 용해시간 2시간, 용해온도 $80^{\circ}C$, 광석입도 $100\;{\mu}m$ 였으며, 최적 용해조건에서 98%의 Mg를 용해할 수 있었다. Si, Al 성분들은 대부분 용해잔사에 존재하므로 용해 후 여과를 통하여 상당량 제거가 가능하였으며, 용해된 불순물들은 용액의 pH 조절을 통하여 금속 수산화물로 석출시켰다. 또한 석출된 금속 수산화물을 응집, 침강시키기 위하여 고분자 응집제를 사용하였으며, 최적 응집제 주입 조건은 비이온계 고분자 응집제 1 mg/100 ml이었다.

Removal of heavy metals in electroplating wastewater by powdered activated carbon (PAC) and sodium diethyldithiocarbamate-modified PAC

  • Kim, Tae-Kyoung;Kim, Taeyeon;Choe, Woo-Seok;Kim, Moon-Kyung;Jung, Yong-Jun;Zoh, Kyung-Duk
    • Environmental Engineering Research
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    • 제23권3호
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    • pp.301-308
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    • 2018
  • We investigated simultaneous removal of heavy metals such as Cr, Ni, and Zn by adsorption onto powdered activated carbon (PAC) and PAC modified with sodium diethyldithiocarbamate (PAC-SDDC). Modification of PAC was confirmed by Fourier transform infrared spectroscopy and Scanning electron microscopy and energy dispersive X-ray spectroscopy. Both PAC and PAC-SDDC reached adsorption equilibrium within 48 h, and the adsorption kinetics followed a pseudo-second order reaction kinetics. The removal of metals was enhanced with increasing both adsorbent dosage and followed the descending order of Cr > Ni > Zn for PAC and Cr > Zn > Ni for PAC-SDDC, respectively. Adsorption kinetics followed pseudo-second order kinetics. Adsorption kinetic results were well fitted by the Freundlich isotherm except for Cr adsorption onto PAC. The optimum pH for heavy metal adsorption onto PAC was 5, whereas that for PAC-SDDC ranged from 7 to 9, indicating that modification of PAC with SDDC significantly enhanced heavy metal adsorption, especially under neutral and alkaline pH conditions. Our results imply that SDDC modified PAC can be applied to effectively remove heavy metals especially Cr in plating wastewaters without adjusting pH from alkaline to neutral.

감마선 및 EMS처리에 의한 유채(Brassica napus L.)와 갓(Brassica juncea L.)의 유묘 감수성 평가 및 형태적 변이체 선발 (Determination of Seedling Sensitivity and Selection of Morphological Mutants by Treatments of Gamma-Ray and EMS in Rapeseed and Leaf Mustard)

  • 강은선;김준수;은종선
    • 방사선산업학회지
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    • 제8권2호
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    • pp.111-121
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    • 2014
  • This study was aimed to select useful mutants of rapeseed (Brassica napus L.) and leaf mustard (Brassica juncea L.), the seeds of three lines S-14, S-27, and S-28 were treated with gamma-ray and EMS. The optimum ranges of gamma-ray dose and EMS concentration to enlarge the characteristic morphological variations were also separately investigated. The survival rates of S-28 only linearly decreased with increasing the gamma-ray dose. The overall growth parameters decreased of gamma-ray dose in all three lines of S-14, S-27, and S-28. The reduction dosage 50 of gamma-ray was identified as 1,200 Gy for S-14 leaf mustard, while those of S-27 and S-28 rapeseed lines were appeared as same 1,000 Gy. The emergence rates of S-14 and S-27 showed no significant differences by EMS treatment, while the growth of all three lines were significantly decreased. The reduction concentration 50 in S-14 could not be determined, demonstrating that this leaf mustard line is presumably insensitive to mutagenic EMS, while those in S-27 and S-28 were identified as 3.0 and 2.5%, respectively, showing that these rapeseed lines possess higher sensitivity to EMS than S-14. Various morphological characteristics of $M_1$ generation obtained from mutagen treatment were elaborately investigated for further maintenance of $M_2$ generation. In $M_2$ generation variants showing short stem, yellow color in seed coat, chlorophyll deficiencies in leaf or pod, abnormal flower color were selected as potentially useful mutants for breeding.

Research on the factors affecting the development of shrinkage cracks of rammed earth buildings

  • Zhao, Xiang;Cai, Hengli;Zhou, Tiegang;Liu, Ling;Ding, Yijie
    • Earthquakes and Structures
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    • 제20권4호
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    • pp.365-375
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    • 2021
  • Rammed earth (RE) buildings have existed all over the world for thousands of years, and have gained increasing attention because of its sustainable advantages, however, the shrinkage cracks reduce its bearing capacity and seriously affect its durability and applicability. In this study, the shrinkage cracks test was carried out to investigate the effects of initial water content, proportion of sand and gravel, compaction degree, thickness and the additives (polypropylene fiber, cement and sodium silicate) of shrinkage cracks in RE buildings, ten groups of RE samples were prepared and dried outdoors to crack. Four quantitative parameters of geometrical structure of crack patterns were used to evaluate the development of cracks. The results show that the specimens cracking behavior and the geometrical structure of crack patterns are significantly influenced by these considered factors. The formation of crack can be accelerated with the increase of initial water content and thickness of specimen, while restricted with the increase of the compaction degree and the proportion of sand and gravel. Moreover, the addition of 1% polypropylene fiber, 10% cement and 0.5 volume ratio sodium silicate can significantly restrain the form and development of cracks. In RE construction, these factors should be considered comprehensively to prevent the harm caused by shrinkage cracks. Further works should be carried out to obtain the optimum dosage of the additives, which can benefit the construction of RE buildings in future.