• Tribology and Lubricants
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• v.34 no.4
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• pp.138-145
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• 2018

Elliptical bearings are widely used for large steam turbines owing to their excellent load carrying capacity and good dynamic stability. Power loss in bearings is an extremely important parameter, especially for high turbine capacities. Optimization of operation conditions and design variables such as bearing clearance and bearing length can reduce the power loss in elliptical bearings. Although changes in the oil supply method have served to increase the efficiency of the tilting pad journal bearing, it has not explicitly improved elliptical bearings. In this study, we verify the static characteristics of an elliptical bearing by changing the direction of oil supply. We evaluate the bearing power loss and bearing metal temperature, and compare the bearing performance and reliability in different test cases. The direction of oil supply is $90^{\circ}$ (9 o'clock) and $270^{\circ}$ (3 o'clock) when the rotor rotates in a counterclockwise direction. We use an elliptical bearing with an inner diameter and active length of 220.30 and 110.00 mm, respectively. Bearing power loss and bearing metal temperatures are measured and evaluated by rotor rotational speed, oil flow rate, and bearing load. The results reveal a 20 reduction in the power loss when the direction of oil supply is 90. Furthermore, the oil film on the upper part of the bearing has a high temperature when the direction of oil supply is $90^{\circ}$. In contrast, when the direction of oil supply is $270^{\circ}$, the oil film on the upper part of the bearing is relatively cold.

• Korean Journal of Food Science and Technology
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• v.36 no.3
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• pp.403-409
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• 2004

Onion, licorice, abgelia root, Chinese date, pine needle, and mulberry leaf were used to extract functional components far onion complex drink. No differences were observed between water extraction at room temperature and methanol extraction in electron-donating ability (EDA), thiosulfinate content (TSC), and ascorbic acid content (AAC), whereas water extraction resulted in higher nitrite-scavenging ability at pH 1.2 and 3.0, and lower superoxide dismutase-like ability than methanol extraction. Level of water extracts prepared by hard-boiling for 3 hr at above $100^{\circ}C$ was higher in all functional abilities except TSC than those prepared at room temperature. Optimal conditions for water extraction and storage were $100-120^{\circ}C$ for 6 hr and low-temperature storage, respectively.

• Journal of Korea Water Resources Association
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• v.39 no.6 s.167
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• pp.503-510
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• 2006

Riverbank filtration is a natural process, using alluvial aquifers to remove contaminants and pathogens in river water for the production of drinking water. Back analysis which minimizes the objective function that is typically the sum of squares of the differences between the calculated and measured quantities is used for the estimation of aquifer parameters. In this study, transmissivity is back-analysed using the BFGS (Broyden-Fletcher-Goldfarb-Shanno) scheme for optimization. MOC is used to obtain calculated groundwater level. The developed inverse model was applied to Dae-san, Chang-won city where riverbank filtration is being undertaken. The model showed good convergence behavior for different groundwater conditions. The performance of the model was better than a widely-used commercial software package in terms of error between calculated and observed groundwater level.

• Journal of Microbiology and Biotechnology
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• v.26 no.9
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• pp.1570-1578
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• 2016

Commercial application of laccase is often hampered by insufficient enzyme stocks, with very low yields obtained from natural sources. This study aimed to improve laccase production by mutation of a Coriolopsis gallica strain and to determine the biological properties of the mutant. The high-yield laccase strain C. gallica TCK was treated with N-methyl-N-nitro-N-nitrosoguanidine and ultraviolet light. Among the mutants isolated, T906 was found to be a high-production strain of laccases. The mutant strain T906 was stabilized via dozens of passages, and the selected ones were further processed for optimization of metallic ion, inducers, and nutritional requirements, which resulted in the optimized liquid fermentation medium MF9. The incubation temperature and pH were optimized to be 30℃ and 4.5, respectively. The mutant strain T906 showed 3-times higher laccase activity than the original strain TCK under optimized conditions, and the maximum laccase production (303 U/ml) was accomplished after 13 days. The extracellular laccase isoenzyme 1 was purified and characterized from the two strains, respectively, and their cDNA sequence was determined. Of note, the laccase isoenzyme 1 transcription levels were overtly increased in T906 mycelia compared with values obtained for strain TCK. These findings provide a basis for C. gallica modification for the production of high laccase amounts.

• Journal of Microbiology and Biotechnology
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• v.28 no.3
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• pp.439-447
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• 2018

The aromatic compound p-hydroxybenzoate (PHBA) is an important material with multiple applications, including as a building block of liquid crystal polymers in chemical industries. The cytochrome P450 (CYP) enzymes are beneficial monooxygenases for the synthesis of chemicals, and CYP53A15 from fungus Cochliobolus lunatus is capable of executing the hydroxylation from benzoate to PHBA. Here, we constructed a system for the bioconversion of benzoate to PHBA in Escherichia coli cells coexpressing CYP53A15 and human NADPH-P450 oxidoreductase (CPR) genes as a redox partner. For suitable coexpression of CYP53A15 and CPR, we originally constructed five plasmids in which we replaced the N-terminal transmembrane region of CYP53A15 with a portion of the N-terminus of various mammalian P450s. PHBA productivity was the greatest when CYP53A15 expression was induced at $20^{\circ}C$ in $2{\times}YT$ medium in host E. coli strain ${\Delta}gcvR$ transformed with an N-terminal transmembrane region of rabbit CYP2C3. By optimizing each reaction condition (reaction temperature, substrate concentration, reaction time, and E. coli cell concentration), we achieved 90% whole-cell conversion of benzoate. Our data demonstrate that the described novel E. coli bioconversion system is a more efficient tool for PHBA production from benzoate than the previously described yeast system.

• Journal of Korean Society of Environmental Engineers
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• v.39 no.8
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• pp.456-461
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• 2017

In this study, nano fibers with various physical properties were materialized by using a variety of polymers [PAN (Polyacrylonitrile), PU (Polyuretane), PSU (Polysulfone)] which are raw materials of dope solution manufactured for electrospinning and solvents [NMP (N-methyl-2 pyrrolidone), DMF (Dimethylformamide)] and evaluated characteristics of their flux and SS (Suspended Solids) separation and then ascertained application of manufactured fibers as separation membrane for water treatment. In this study, analysis of surface of manufactured material was carried out through SEM analysis to ascertain the cause of flux and SS separation performance by checking diameter, uniformity and straightness of fiber. If additive is used in manufacturing nano fiber water treatment separation membrane, it is expected to solve problems such as membrane fouling and mechanical strength and to be used as basic factor for manufacturing separation membrane with catalyst function added.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.62 no.5
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• pp.696-704
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• 2013

In this paper, we introduce the LED emotional lighting system realized with the aid of both intelligent algorithm and RGB LED combined with White LED. Generally, the illumination is known as a design factor to form the living place that affects human's emotion and action in the light- space as well as the purpose to light up the specific space. The LED emotional lighting system that can express emotional atmosphere as well as control the quantity of light is designed by using both RGB LED to form the emotional mood and W LED to get sufficient amount of light. RBFNNs is used as the intelligent algorithm and the network model designed with the aid of LED control parameters (viz. color coordinates (x and y) related to color temperature, and lux as inputs, RGBW current as output) plays an important role to build up the LED emotional lighting system for obtaining appropriate color space. Unlike conventional RBFNNs, Fuzzy C-Means(FCM) clustering method is used to obtain the fitness values of the receptive function, and the connection weights of the consequence part of networks are expressed by polynomial functions. Also, the parameters of RBFNN model are optimized by using PSO(Particle Swarm Optimization). The proposed LED emotional lighting can save the energy by using the LED light source and improve the ability to work as well as to learn by making an adequate mood under diverse surrounding conditions.

• Journal of Microbiology and Biotechnology
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• v.10 no.5
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• pp.728-732
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• 2000

Propagation of Anagrapha falcifera nuclear polyhedrosis virus(AfNPV) was investigated using well-plates and split-flow air-lift bioreactors. In well-plate experiments, the effects of pH, cell density at a point of infection, serum concentration, DEAE-dextran, and lipid on virus propagation were all closely examined. The AfNPV titer in well-plates was optimal at pH 6.8 and $3{\times}10^6$ cells/$cm^2$. The virus titer was not dramatically affected when the fetal bovine serum concentration was reduced from 10% to 5%. The addition of cholesterol at AfNPV infection of Sf21 cells enhanced the virus titer, whereas the addition of DEAE-dextran did not improve the titer. The AfNPV titer ($3.8{\times}10^7$ $TCID_{50}/ml$) at optimized conditions for well-plate experiments was 2.5-fold higher than for the control. In bioreactor experiments, the AfNPV titer showed its maximum level at air flow rates of 20-40 ml/min. In a split-flow air-lift bioreactor, AfNPV titer ($2.3{\times}10^7\;TCID_{50}/ml$) was 1.5-fold higher than the control when the culture was at pH 6.8 and supplemented with 0.34 mM cholesterol.

• Journal of Microbiology and Biotechnology
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• v.24 no.6
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• pp.823-834
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• 2014

In order to solve the shortage of natural Tripterygium wilfordii Hook. f. plant resource for the production of the important secondary metabolites triptolide and wilforine, hairy roots were induced from its root calli by Agrobacterium rhizogenes. Induced hairy roots not only could be maintained and grown well in hormone-free half-strength Murashige and Skoog medium but also could produce sufficient amounts of both triptolide and wilforine. Although hairy roots produced approximately 15% less triptolide than adventitious roots and 10% less wilforine than naturally grown roots, they could grow fast and could be a suitable system for producing both secondary metabolites compared with other tissues. Addition of $50{\mu}M$ methyl jasmonate (MeJA) could slightly affect hairy root growth, but dramatically stimulated the production of both triptolide and wilforine, whereas $50{\mu}M$ salicylic acid had no apparent effect on hairy root growth with slightly stimulatory effects on the production of both secondary metabolites. Addition of precursor nicotinic acid, isoleucine, or aspartic acid at the concentration of $500{\mu}M$ had varying effects on hairy root growth, but none of them had stimulatory effects on triptolide production, and only the former two had slightly beneficial effects on wilforine production. The majority of triptolide produced was secreted into the medium, whereas most of the produced wilforine was retained inside of hairy roots. Our studies provide a promising way to produce triptolide and wilforine in T. wilfordii hairy root cultures combined with MeJA treatment.

• Journal of Microbiology and Biotechnology
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• v.30 no.1
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• pp.136-145
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• 2020

Chlorella sp. HS2, which previously showed excellent performance in phototrophic cultivation and has tolerance for wide ranges of salinity, pH, and temperature, was cultivated heterotrophically. However, this conventional medium has been newly optimized based on a composition analysis using elemental analysis and ICP-OES. In addition, in order to maintain a favorable dissolved oxygen level, stepwise elevation of revolutions per minute was adopted. These optimizations led to 40 and 13% increases in the biomass and lipid productivity, respectively (7.0 and 2.25 g l^-1d^-1 each). To increase the lipid content even further, 12 h heat shock at 50℃ was applied and this enhanced the biomass and lipid productivity up to 4 and 17% respectively (7.3 and 2.64 g l^-1d^-1, each) relative to the optimized conditions above, and the values were 17 and 14% higher than ordinary lipid-accumulating N-limitation (6.2 and 2.31 g l^-1d^-1). On this basis, heat shock was successfully adopted in novel Chlorella sp. HS2 cultivation as a lipid inducer for the first time. Considering its fast and cost-effective characteristics, heat shock will enhance the overall microalgal biofuel production process.