• 한국양식학회:학술대회논문집
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• 한국양식학회 2003년도 추계학술발표대회 논문요약집
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• pp.38-38
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• 2003

Blocking the first mitotic cleavage of the zygote is a key tool for chromosome-set manipulations in fish. We developed an improved method for inducing tetraploidy by blocking the mitosis with a combination of heat shock at 40.5$^{\circ}C$ for 1, 2 or 3 min followed by cold shock at $1.5^{\circ}C$ for 30, 45 or 60 min. When applied during the first cleavage metaphase of mud loach (Misgurnus mizolepis) zygotes, the optimal combination was heat for 2 min followed by cold for 45 min. At 1 month, the frequency of 4N survivors and the yield from total eggs fertilized was 55.7% and 14.4%, respectively, compared to heat shock alone with 20.0% efficiency and 3.6% yield. The effectiveness of the procedure was confirmed by diploid mitotic gynogenesis using transgenic markers. The overall yield of homozygous diploids, 34.0%, was better than that for single heat shock, 17.3%. The tetraploids and homozygous diploids had higher early mortality than normal diploid controls. However at 1 month, the viability of the tetraploids was the same as normal diploids. For gynogenetic diploids, the survival was similar to normal diploids after 3 months. The high efficiency of this new protocol extends the opportunity to study polyploidy in basic and applied research.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2005년도 춘계학술대회 논문집
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• pp.1963-1966
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• 2005

Degenerative lumbar spinal stenosis(DLSS) is a disease inducing low back pain, leg pain, convulsion, numbness, and neurogenic claudication from compression of nerve root. Intervertebra fixation was reported to increase the degenerative of neighbor region after treatment. Recently, a new surgical technique of inserting a fixator between interspinous processes has been introduced. The purpose of this study is to design of the interspinous process fixator with flexibility to complement the trouble of using fixator in DLSS. This study evaluated the existing fixator through the mechanical test and modified fixators using the finite element analysis(FEA). Displacement, stiffness and Von-Mises stress were found to have similar values to those obtained from the mechanical test and the FEA in the biomechanical loading condition. Effects of variation in length and thickness were investigated to design an optimal fixator.

• 한국추진공학회:학술대회논문집
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• 한국추진공학회 2005년도 제25회 추계학술대회논문집
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• pp.141-146
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• 2005

본 논문은 램제트/스크램제트 엔진에 대한 이론적인 해석 결과를 보여준다. 본 연구의 주요 목적은 램제트/스크램제트 엔진의 성능변수에 대하여 해석적 연구를 통해 물리적인 이해와 기초를 제시하는 것이다. 램제트/스크램제트 성능에 있어서 비행 마하수와 흡입구, 연소특성 등의 영향에 대하여 해석적 연구를 수행하였으며, 일정한 압력 또는 일정한 단면적을 가진 연소기에 대하여 사이클 해석을 통해 비교/분석하였다. 또한 연소기 입구에서의 최적 마하수에 대하여 연구하였다.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2000년도 추계학술대회 논문집
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• pp.393-396
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• 2000

Spent fuel rod cutting device should cut a spent fuel rod to an optimal size in order to fast decladding operation. In this paper, for developing spent fuel rod cutting device with cutter blade, rod properties such as dimension and material of zircaloy tube and fuel pellet are investigated at first and then, various methods of existing cutting devices used commercially are investigated and their performance are analyzed and compared. This device is designed to be operated automatically via remote control system considering later use in Hot-Cell (radioactive area) and the mdularization in the structure of this device makes maintenance easy. SUS and Zircaloy-4 are selected as cut material used in the test of spent fuel rod cutting device by cutter blade. In order for constructing the high durable cutter blade, various materials are analyzed in terms of quality, shape, characteristic, and heat treatment, etc. and from these results, spent fuel rod cutting device is designed and manufactured based on the considerations of durability, round shape sustainability of rod cross-section, debris generation, and fire risk, etc.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2001년도 The 6th International Symposium of East Asian Resources Recycling Technology
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• pp.443-447
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• 2001

A laboratory-scale electrodewatering system for enhancing conventional filter pressure dewatering by an electric field has been developed to decrease the water content of sludge generated in the wastewater treatment. It consists of a piston-typed filter press, a power supply and data acquisition system. The offset of electrodewatering is investigated as a function of applied pressure, applied voltage, sludge type and filtration time. Also the optimal conditions for maximizing the dewatering efficiency in the eletrodewatering system are investigated. Electric field strength and mechanical pressure are in the range of from 0 to 120 V/cm and from 98.1 to 392.4 kPa. The dewatering rates increased with increasing electric strength. These experiments produced a final sludge cake with water content of 60 wt% using electrodewatering technology, compared with a 80 wt% using pressure filtration alone. The conventional filtration system using the electrodewatering shows the potential to be effective method for improving dewatering Sludge.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2000년도 춘계학술대회 논문집
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• pp.833-836
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• 2000

The mechanical and thermal load. and thermal softening which is happened by the high temperature of die, in hot and warm forging, cause wear. heat checking and plastic deformation, etc. This study is for the effects of solid lubricants and surface treatments for warm forging die Because cooling effect and low friction are essential to the long lift of dies. optimal surface treatments and lubricants are very important to hot and warm forging Process. The heat that is generated by repeated forging processes. and its transfer are important factors to affect die life. The main factors, which affect die hardness and heat transfer, are surface treatments and lubricants, which are related to thermal diffusion coefficient and heat transfer coefficient, etc. For verifying these. experiments art performed for diffusion coefficient and heat transfer coefficient in various conditions - different initial billet temperatures and different loads. Carbonitriding and ionitriding are used as surface treatments. and oil- base and water-base graphite lubrirants are used. The effects of lubricant and surface treatment for warm forging die lift are explained by their thermal characteristics.

• 한국표면공학회:학술대회논문집
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• 한국표면공학회 2018년도 춘계학술대회 논문집
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• pp.24-24
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• 2018

One of the challenges in tissue engineering is the design of optimal biomedical scaffolds, which can be governed by topographical surface characteristics, such as size, shape, and direction. Of these properties, we focus on the effects of nano - to micro - sized hierarchical surface. To fabricate the hierarchical surface structure on poly(${\varepsilon}$-caprolactone) (PCL) film, we employed a nano/micro-casting technique (NCT) and modified plasma process. The micro size topography of PCL film was controlled by sizes of the micro structures on lotus leaf. Also, the nano-size topography and hydrophilicity of PCL film were controlled by modified plasma process. After the plasma treatment, the hydrophobic property of the PCL film was significantly changed into hydrophilic property, and the nano-sized structure was well developed, as increasing the plasma exposure time and applied power. The surface properties of the modified PCL film were investigated in terms of initial cell morphology, attachment, and proliferation using osteoblast-like-cells (MG63). In particular, initial cell attachment, proliferation and osteogenic differentiation in the hierarchical structure were enhanced dramatically compared to those of the smooth surface.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2004년도 춘계학술대회
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• pp.198-203
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• 2004

In these days, the orthodontic surgery including lingual orthodontics has attracted a person' attention due to its functional and esthetic appreciation. The delivery of the optimal orthodontic treatment is greatly influenced by clinician' ability to predict and control the tooth movement by applying force system to dentition. The skeletal anchorage system with the miniscrew has been used recently in the lingual orthodontics to assist the anchorage control. Precise understanding of the force system produced from the various orthodontic appliances is necessary. However, the qualitative and quantitative effect of the miniscrew has not been identified well. In this paper, three dimensional finite element analysis is introduced on the lingual orthodontics to investigate the effect of anterior retraction force on the miniscrew and transpalatal arch wire. The purpose of this study is to determine the location of the miniscrew and the point of force application of the anchorage system in the lingual orthodontics. The analysis results indicate the efficient position of the miniscrew and the transpalatal arch wire in the lingual orthodontics.

• 한국공작기계학회:학술대회논문집
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• 한국공작기계학회 2005년도 춘계학술대회 논문집
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• pp.185-190
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• 2005

The shot peening process is often used to improve fatigue properties of metal parts. Among them, It is the most use in an auto-component. In order to achieve optimum, repeatable and reliable fatigue enhancement from the shot peening process, the important shot peening parameters must be controlled. In this paper, the optimum peening intensity (Almen intensity) condition is investigated by experiment. The Spur Gear steel was used to investigate shot peening effects. The fatigue life at $\sigma_a=1,050$ and $\sigma_a=1,250MPa$ first gently increases, then drops gently as peening intensity increases compared with unpeened specimen. Experimental results show that the optimum peening intensity range is $0.391\~0.434mmA$..So the fatigue strength and fatigue life have been tremendously increased by optimum-peening treatment. However, the fatigue strength and fatigue life have been decreased by over peeing.

• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2001년도 춘계학술발표대회
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• pp.30-30
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• 2001

The culture of preantral follicles has important biotechnological implications through its potential to produce the large quantity of oocytes for embryo production, transgenesis research, conservation of rare breed, and a potential source of ovarian genetic material. The present study was conducted to establish the optimal conditions of in vitro culture for intact bovine preantral follicles; and to examine the developmental ability of oocytes derived from the in vitro-grown preantral follicles; and to investigate the effects of various concentrations of FSH and LH on these processes. Bovine preantral follicles (150 $\pm$ 1.2${\mu}{\textrm}{m}$), surrounded by theca cell, were isolated enzymetically and mechanically from ovarian cortical slides in Leibovitz L-15 medium containing 1 mg/$m\ell$ collagens and 0.2 mg/$m\ell$ DNase I and cultured for 25 days in the presence of different concentrations of bovine FSH and LH in $\alpha$MEM medium with insulin, transferrin, and selenite. The survival was tested by frypan Blue and Hematoxylin. The survival and growth rates of follicles were higher in FSH treatment groups than these in control (P<0.001), but there were no significant differences between the LH treatment groups and the control. In 25 days, the survival and growth rates of follicles in FSH and LH treatment group (50%, 300$\pm$1.0${\mu}{\textrm}{m}$) were higher than in FSH treatment group (40%, 244$\pm$0.5${\mu}{\textrm}{m}$) and the control group (25%, 160$\pm$ 1.0${\mu}{\textrm}{m}$). Fifty-five percent of healthy antral follicles were obtained, and 60% of the oocytes complete meiotic maturation to the metaphase II stage. Twenty-two percent of the mature oocytes underwent cleavage, and 9% developed to the blastocyst stage. In this study, in vitro-grown oocytes (111 $\pm$ $1.5mutextrm{m}$), under our culture conditions, were not equivalent in size to the in vivo-grown oocytes (130$\pm$1.3${\mu}{\textrm}{m}$). Therefore, these results suggest that bovine preantral follicles with intact theca cell can grow to the antral stage in 25days, and that oocytes from those follicles can acquire the meiotic competence and normally undergo fertilization and development to the blastocyst stage. However, the developmental capacity of in vitro-grown oocytes is presumably not comparable to those of the in vivo counterparts.