• 대한화장품학회지
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• 제13권1호
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• pp.7-14
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• 1987

Production of shikonin derivatives through cell suspension culture of Lithospermum erythrorhizon was investigated. Optimal concentrations of IAA and kinetin on the growth of cell suspension were 0.2 and 0.1 ppm respectively. Pigment content was markedly increased when aluminum oxide was added to the production medium and its optimal concentration was 1.5g/70ml medium. The most effective concentration of IAA was 0.5 ppm and the production of pigment did not depend on the kinetin concentration.

• 식물조직배양학회지
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• 제28권4호
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• pp.215-219
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• 2001

팔레높시스 대량생산시 PLB 증식에 미치는 배지와 배양환경에 대해 조사하였다. PLB 증식량은 MS배지에서 높았지만, 증식된 PLB상태는 NDM배지가 더욱 양호하였다 천연첨가 물인 CW 10% 또는 사과와 감자의 사용은 PLB 증식효율을 향상시키는 데 필수적이었다. 배지내 지지물에 따른 PLB 증신률은 gelrite가 첨가된 고체배지가 흰색과 분홍색계통 모두 액체탈지면 배지보다 증식이 양호하였다. PLB 증식시 적정 sucrose 농도는 10g/L이며 농도가 높아질수록 PLB가 유식물체로 재분화되었고 저농도 일수록 증식과 유식물체의 재분화가 모두 억제되었다. PLB증식을 위한 적정 PPF와 온도는 각각 14.3 $\mu$mol.s$^{-1}$m$^{-2}$ , $25^{\circ}C$이었다.

• Reproductive and Developmental Biology
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• 제30권3호
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• pp.163-167
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• 2006

The purpose of the present study was to establish culture conditions for the in vitro study of the neonatal piglet Sertoli cell. Isolation for the culture of Sertoli cell was established using collagenase and pancreatin digestion of testicular tissues. The effects of various culture media, fetal bovine serum(FBS), follicular stimulating hormone(FSH), epidermal growth factor(EGF) and insulin-transferrin-sodium selenite(ITS) on growth of neonatal piglet Sertoli cells were investigated. The mitogenic effects of Dulbecco's modified Eagle's medium+Ham's F-12 medium was higher than other media used in this experiment. The addition of 1% FBS in cultures was necessary for attachment of Sertoli cell clusters. However, except FBS and EGF, FSH and ITS did not stimulate Sertoli cell proliferation. When Sertoli cells isolated from neonatal piglets were cultured in Dulbecco's modified Eagle's medium+Ham's F-12 medium supplemented with 1% FBS, FSH EGF and ITS, the yield and plating efficiency of Sertoli cells were largely increased. Confluency of Sertoli cells was reached as early as 4 days of culture. The method described here reduces or eliminates many of the drawbacks of the conventional procedures used to isolate and culture of Sertoli cells, thus providing a useful tool in studies of growth kinetics and regulation of cell proliferation in vitro.

• Biotechnology and Bioprocess Engineering:BBE
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• 제5권5호
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• pp.350-354
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• 2000

The growth promotion of a Taxus brevifolia cell suspension culture was investigated using conditioning factors. The conditioning factors produced and secreted from cultured cells usually stimulate cell division and the production of secondary metabolites. Therefore, the effective incubation time for the optimal secretion of conditioning factors was firstly determined for the promotion of cell growth. Conditioned media obtained by cultivating for 2 and 5 days showed the promotion of initial cell growth during the early cell growth period. However, the positive effect of the conditioning factors on the initial cell growth did not continue because of the depletion of the medium nutrients. Accordingly, the addition of a carbon source to the conditioned medium prolonged the positive effect on the cell growth. The addition of sucrose to the conditioned medium resulted in the maximum cell density being reached 4 days earlier compared to the control group and an increased substrate yield.

• Reproductive and Developmental Biology
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• 제34권3호
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• pp.207-211
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• 2010

Current developments in IVF and animal cloning have resulted in increasing demand for large quantities of oocytes and ovarian follicles at specific stages of development. These medical and scientific needs may be met by developing an optimal culture system for preantral follicles. In this study, we investigated the growth of porcine preantral follicle cultures in different media and in the presence and absence of serum. Follicles were manually dissected from ovaries obtained from prepubertal gilts at a local slaughterhouse, and cultured for 3 days in M199 or NCSU23 medium supplemented with porcine FSH, transferrin, L-ascorbic acid and insulin. Follicle diameters were measured on day 1 and 3 of culture. In Experiment 1, the effect of supplementing culture medium with fetal calf serum (FCS) on porcine preantral follicle growth was examined. In the group of cultures supplemented with FCS, follicle diameter after 3 days of culture, survival rate and antrum formation rate in the FCS group were significantly higher than those of the control group. In Experiment 2, the effects of culture medium (M199 and NCSU23) on follicle growth were compared. Follicle diameters were increased in the M199 group, compared with those in NCSU23 (p<0.05), but we observed no significant differences in survival and antrum formation rates between cultures grown in the two media. In conclusion, supplementation of the culture medium with serum enhances preantral follicle growth and antrum formation, and M199 is superior to NUSU23 for porcine preantral follicle culture in vitro.

• Mycobiology
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• 제33권4호
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• pp.230-234
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• 2005

Oudemansiella radicata, one of edible mushrooms belonging to Tricholomataceae of Basidiomycota, has been known to exhibit outstanding therapeutic effects on the hypertension caused by high blood pressure and inhibitory effects on the sarcoma 180 and Erhrlich carcinoma of mice. As one of preliminary experiments for producing fruiting-body of O. radicata, this study was carried out to obtain the basic information for culture conditions of mycelial growth of the fungus. The optimal temperature and pH for the mycelial growth were $25^{\circ}C$ and pH 6, respectively. The medium for favorable mycelial growth of O. radicata was shown in the Lilly medium, whereas compact mycelial density was found in Hamada medium. The carbon and nitrogen sources promoting for mycelial growth of O. radicata were xylose and alanine, respectively. The optimum C/N ratio was about 20 : 1 in case that 3% glucose was supplimented to the basal medium as a carbon source.

• 한국미생물·생명공학회지
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• 제23권6호
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• pp.647-651
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• 1995

A tylosin-hyperproductive mutant of S. fradiae MNU20 was isolated among 3500 strains obtaincd from either MNNG- or UV-treated Streptomyces fradiae NRRL2702. The composition of optimal medium for tylosin production was formulated as followed as: 4 g soluble starch, 1 g glucose, 1 g corn steep liquor, 7.5 ml soy bean oil, 0.2 g KH$_{2}$PO$_{4}$, 1 g Na$_{2}$S$_{2}$O$_{3}$$\cdot $5H$_{2}$O, 2 g CaCO$_{3}$, 2 g NaCl, 0.001 g CoCl$_{2}$$\cdot $6H$_{2}$O in 1 liter of distilled water. With the optimal medium, S. fradiae MNU20 was able to produce 159 mg tylosin (g biomass)$^{-1}$, indicating that tylosin productivity of Streptomyces fradiae NRRL2702 was increased 14 times higher by mutation. When the effect of valine, succinate, and natural zeolite on tylosin production was investigated by using the optimal medium, these substances essentially enhanced tylosin production and their addition time during culture period appeared to be critical for the increase of tylosin production.

• 한국균학회지
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• 제46권3호
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• pp.333-344
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• 2018

점박이응애와 복숭아혹진딧물 그리고 잿빛곰팡이병균에 대해 우수한 살충 및 항균활성을 지닌 4가지 곤충병원성 곰팡이에 대해 효율적인 배양 배지 선발 및 다른 식물병원균에 대한 항균활성을 검정하였다. 효율적인 배지 선발은 출아포자 생산량과 배양액의 항진균활성 검정을 통해 선발하였으며, 그 결과 Beauveria bassiana 2R-3-3-1, Metarhizium anisopliae 4-2, SD3의 경우 GY배지가, B. bassiana SD15의 경우에는 PDB 배지가 적절한 배지로 선발되었다. 다른 식물병원균에 대한 항균활성 검정 결과, 4개 균주 모두 Colletotrichum acutatum과 Sclerotinia sclerotiorum에 대해서는 항진균 활성을 보였으나, Phytophthora capsici와 Colletotrichum fructicola에 대해서는 M. anisopliae SD3만 P. capsici에 대해 높은 항진균 활성을 나타내었고 다른 균주들의 활성은 미미하였다. Clavibacter michiganensissubsp. michiganensis에 대한 항세균 활성은 Metarhizium속의 2균주는 높은 활성을 보였으나 Beauveria속의 2 균주들은 활성을 보이지 않았다. 이상의 결과로 해충방제에 효과적인 곤충병원성 곰팡이가 다양한 식물병원균에 대해서도 효과적인 방제제로 이용될 수 있음을 확인할 수 있었다.

• 한국미생물·생명공학회지
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• 제17권5호
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• pp.510-518
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• 1989

농산 폐자원 중에서도 감귤 가공공정에 따라 부생되는 감귤과피를 발효 기질로서의 이용을 목적으로 분리균 Asp. niger를 사용하여 구연산 발효에 미치는 배양조건 및 영향인자를 조사하였다. 그 결과 고 체 배양에서의 최적조건 즉 감귤과피와 물을 동량 혼합하고 여기에 NH$_4$NO$_3$0.2%, MgSO$_4$7$H_2O$ 0.1%, Methanol 2.5%를 첨가하여 PH4.5로 조절한 다음포자 현탁액(1x$10^8$ spore/$m\ell$) 2%를 접종하여 균체증식 초기에 35$^{\circ}C$, 20시간 배양하고 4시간 저온 처리한 다음 구연산 생성기에서 3$0^{\circ}C$로 48시간 배양했을 때 당에 대해 80.4%의 높은 수율의 구연산을 얻었다.

• Journal of Plant Biotechnology
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• 제5권1호
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• pp.1-6
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• 2003

The adventitious root of Panax ginseng C.A. Meyer is regarded as an efficient alternative to cell culture or hairy root culture for biomass production due to its fast growth and stable metabolite production. To determine optimal culture conditions for the bioreactor culture of ginseng roots, experiments have been conducted on physical and chemical factors such as bioreactor type, dissolved oxygen, gas supply, aeration, medium type, macro- and micro-elements, medium supplement during culture period, sucrose concentration, osmotic agents, medium pH and light. Elicitation is a key step to increase ginsenoside accumulation in the adventitious roots but biomass growth is severely inhibited by elicitor treatment. To obtain high ginsenoside content with avoiding biomass decrease, we applied two-stage bioreactor culture system. Ginseng adventitious roots were cultured for 40 days to maximize biomass increase followed by elicitation for 7 days to enhance ginsenoside accumulation. We also experimented on types and concentrations of jasmonate to determine optimal elicitation methods. In this paper, we discussed several factors affecting the root propagation and ginsenoside accumulation. Based on the results obtained from previous experiments we have established large-scale bioreactor system (1 ton-10 ton) for the efficient production of ginseng adventitious roots and bioactive compounds including ginsenoside. Still, experiments are on going in our laboratory to determine other bioactive compounds having effects on diet, high blood pressure, DPPH elimination and increasing memories.