• Biotechnology and Bioprocess Engineering:BBE
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• v.7 no.4
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• pp.212-215
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• 2002

Tyrosinase transcript In the blood Is known as the marker of malignant melanoma and it has been often determined by using reverse transcription-polymerase chain reaction (RT-PCA) . However, after the PCR process, the quantification of amplified CDMA by the gel electrophoresis is not reliable and time-consuming. for this reason, we tried to quantify the PCR product using a cuvette-type biosensor, where the oligonucleotide probe was immobilized on the cuvette surface and the single strand CDMA, the denatured PCH product, was then hybridized onto the immobilized probe to give a response signal. The response was Immediate and takes 15 min to obtain a stable signal. The biosensor was much more sensitive comparing to the gel electrophoresis method. The quantification of PCR product using a cuvette-type biosensor was feasible and rapid.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.61 no.3
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• pp.433-436
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• 2012

We propose a novel micro surface plasmon resonance (SPR) sensor system based on polymer materials. The proposed SPR system consists of the incident medium with polymer waveguide and the gold thin film for sensing area. Using a polymer optical waveguide instead of a prism in SPR sensing system offers miniaturization, low cost, and potable sensing capability. The whole device performance was analyzed using the finite-difference time domain method. The optimum gold thickness in the attenuated total reflection mirror of polymer waveguide is around 50 nm and the resonance angle to generate surface plasmon wave is 68 degrees.