• The Plant Pathology Journal
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• 제32권3호
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• pp.190-200
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• 2016

Xanthomonas oryzae pv. oryzae (Xoo) causes bacterial leaf blight (BLB) in rice (Oryza sativa L.). In this study, we investigated the effect of a mutation in opsX (XOO1056), which encodes a saccharide biosynthesis regulatory protein, on the virulence and bacterial chemotaxis of Xoo. We performed DNA micro-array analysis, which showed that 63 of 2,678 genes, including genes related to bacterial motility (flagellar and chemotaxis proteins) were significantly downregulated ($<\;-2\;log_2$ fold changes) by the mutation in opsX. Indeed, motility assays showed that the mutant strain was nonmotile on semisolid agar swarm plates. In addition, a mutant strain (opsX::Tn5) showed decreased virulence against the susceptible rice cultivar, IR24. Quantitative real-time RT-PCR reaction was performed to confirm the expression levels of these genes, including those related to flagella and chemotaxis, in the opsX mutant. Our findings revealed that mutation of opsX affects both virulence and bacterial motility. These results will help to improve our understanding of Xoo and provide insight into Xoo-rice interactions.

• Journal of applied mathematics & informatics
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• 제17권1_2_3호
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• pp.797-802
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• 2005

We generate simplex polynomials by using a method, which produces an OPS in (d + 1) variables from an OPS in d variables and the Jacobi polynomials. Also we obtain a partial differential equation of the form $${\Sigma}_{i,j=1}^{d+1}\;A_ij{\frac{{\partial}^2u}{{\partial}x_i{\partial}x_j}}+{\Sigma}_{i=1}^{d+1}\;B_iu\;=\;{\lambda}u$$, which has simplex polynomials as solutions, where ${\lambda}$ is the eigenvalue parameter.

• 정보과학회 컴퓨팅의 실제 논문지
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• 제22권11호
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• pp.547-558
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• 2016

다양한 ICT 인프라 기술들을 종합적으로 활용하는 클라우드의 대표적인 오픈소스 프로젝트인 오픈스택을 활용하여 멀티사이트 기반의 클라우드 인프라 구축 시 설치 복잡성 및 지리적인 제약으로 인한 인적, 시간적인 비효율성을 내포한다. 이러한 비효율성을 해소하기 위해 멀티사이트 환경의 오픈스택 실증 테스트베드인 OF@KOREN SmartX 놀이터 (Playground)를 대상으로 리눅스 및 오픈스택을 설치/설정을 자동화하는 도구를 데브옵스 (DevOps) 개발 방법론에 따라 점진적으로 개발해왔다. 하지만 이전 개발도구들이 고정된 형태의 프로비저닝만을 제공한다는 한계를 해결하고자 본 논문에서는 소프트웨어 정의 인프라의 자원관리 구도에 따라 자동 설치/설정도구를 엮어 Playground 수준의 프로비저닝을 수행하는 SmartX 프로비저닝 프레임워크의 프로토타입을 설계, 개발한다. 그리고 멀티사이트 오픈스택 클라우드를 자동으로 구축하는 과정을 제시함으로써 프레임워크의 효율적인 놀이터 프로비저닝 기능에 대해 검증한다.

• Asian-Australasian Journal of Animal Sciences
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• 제21권10호
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• pp.1404-1410
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• 2008

This study was performed to clarify whether the variation of stress related heat shock protein 70 (HSP70) (GenBank X68213) gene was associated with the nuclear morphological change of in vitro maturation and in vitro capacitation in oocytes of pig ovaries obtained at the slaughterhouse. The nucleic acid substitution of C to G at the 483rd position was found out in HSP70 K1 (290-512) from X68213. The ovaries were categorized into CC, CG, and GG genotypes using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) (BsiHKA I). After the second in vitro maturation of immature fresh oocytes, the relation of nuclear morphological change in oocytes with the genotype of HSP70 K1 gene was such that the MII ratios of the genotype GG and CG (46.93% and 42.20%, respectively) were significantly higher than that of the CC genotype (10.71%) (p<0.05). With respect to in vitro maturation of frozen-thawed oocytes by an open pulled straw (OPS) method, the percentage of oocytes matured to MII stage of the CG genotype showed a higher trend than CC and GG genotypes. After the in vitro maturation of immature fresh oocytes and frozen-thawed oocytes by the OPS method, the relation of the pronuclei change in oocytes matured in vitro with HSP70 genotype was assessed, and the result showed that the enlarged sperm heads (ESH) of matured fresh oocytes and frozen-thawed oocytes were 80.0% and 60.0% in the CC genotype, respectively. The CC genotype group had a significantly higher rate of ESH than the CG and the GG genotype group (p<0.05). The ratios of polyspermic invasion were not different among HSP70 of the three genotypes. It was considered that the rate of in vitro maturation of fertilized oocytes was expected to differ according to genotype of the stress related gene.

• 한국식품저장유통학회지
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• 제18권5호
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• pp.721-728
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• 2011

Camellia oil과 palmitic, 그리고 stearic ethyl ester를 기질로 하고 Lipozyme TLIM을 촉매로 이용하여 interesterification을 통한 POS를 많이 함유한 반응물을 합성하고자 하였다. 반응 조건(반응효소비율, 반응시간, 기질의 비율)을 독립변수로 하여 중심 합성 계획에 의한 반응 표면 분석을 통하여 POS의 함량은 높이고, sn-2 position의 acyl migration을 낮추어 합성하는 최적화 조건을 얻었다. POS를 많이 함유한 반응물의 합성 최적화 조건은 반응 효소양($X_1$)= 5.9%, 반응시간($X_2$)= 60 min, 그리고 기질비율($X_3$)= 1:3(1 mole의 camellia oil: 3 mole의 palmitic ethyl ester + 3 mole의 stearic ethyl ester)이었다. 이와 같은 조건으로 획득된 실재 효소반응 합성물의 TAG-P/O/S(palmitic, stearic과 oleic 지방산을 각각 1 분자씩 어느 위치이던지 함유한 TAG, 즉 POS 및 PSO/OSP/OPS/SPO)의 합성율($Y_1$)은 20.19%이었고, 이때 acyl migration 등에 의하여 sn-2 위치에 결합(이동)한 palmitic과 stearic acid의 양(P/S-sn-2)인 $Y_2$는 12.71% 이었다.