• 한국응용곤충학회지
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• 제36권4호
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• pp.331-340
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• 1997

곤충 유전자를 이용한 항세균성 펩타이드 생산 및 응용에 관한 기초 연구로서 비병원성 세균(Escherichia coli K12)으로 면역 반응을 유도한 누에로부터 발현량이 증가하는 유전자 중 Hyalophora cecropia의 attacin과 cDNA 상동성을 나타내는 BmInc6 클론을 분리하고 그 특성을 조사하였다. BmInc6 cDNA의 전체염기서열을 분석한 결과 그 크기는 852 bp이고, 35번째 염기에서 변역이 개시되어 679 bp 위치에서 종결되는 open reading frame을 가지며 812번째 위치에 잠정 전사 종결 신호의 존재가 확인되었다(GenBank, AF005384). BmInc6에 의해 coding되는 아미노산은 214개이며, hydropathy 분석 결과 친수성을 나타내는 단백질이었다. 그리고 BmInc6 유전자에 의해 연역되는 펩타이드를 nuecin으로 명명하였다. Nuecin 유전자를 baculovirus 발현 백터계를 이용하여 곤충세포주에서 발현시킨 결과 전사체의 크기는 약 950 bp였고, 세포내 벌현 펩타이드의 분자량은 약 23 kDa이었다. 세포내에서 발현된 nuecin 전구체로 추정되는 23 kDa 펩타이드는 세포외로 분비되는 과정에서 약 3 kDa의 signal 펩타이드가 제거됨으로서 약 20 kDa의 성숙 nuecin으로 된다는 사실을 단백질 전기영동상으로 확인하였다. Nuecin 단백질의 항세균 활성을 수종의 그람 음성 및 양성 세균에 대해 검정한 결과, 특히 E. coli와 Bacillus subtilis에 높은 활성을 나타내었으며, attacin이 한정된 그람 음성 세균에만 항세균 활성을 나타내는데 비해 nuecin은 그람 음성은 물론 그람 양성에도 항세균 활성을 나타내어, 보다 넓은 항세균 스펙트럼을 가지는 것을 알 수 있었다.

• 한국도서관정보학회지
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• 제51권4호
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• pp.185-210
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• 2020

도서관은 코로나 19와 같은 예기치 못한 위기에 직면하게 되었으며, 이에 대한 대응 전략으로 비대면 온라인서비스를 강화하였다. 이에 본 연구에서는 급격하게 변화하는 외부환경 변화와 현재의 위기를 극복하기 위한 방안을 제시하고자 하였다. 이를 위해 통합사이트로 서비스되는 공공도서관 288개관의 홈페이지를 대상으로 자료검색과 전자도서관, 도서관서비스, 문화행사, 열린공간에 대한 운영 현황을 조사하였으며, 분석 및 파악하였다. 이를 기반으로 공공도서관의 온라인서비스가 포스트 코로나 시대에 어떠한 의미를 지니며 시사점은 무엇인지를 파악하였다. 그 결과, 첫째, 비대면 문화의 확산에 따른 온라인 자료검색 서비스 이용률 증가, 둘째, 전자도서관의 서비스 확대, 셋째, 비대면, 온라인 서비스 다양화, 넷째, 온라인 문화행사 서비스 확대, 다섯째, 열린공간 서비스 다양화, 여섯째, 출입 기반 무인 대출반납 인공지능 시스템 도입과 일곱째, VR, AR 및 MR을 통한 체험형 문화 지원 서비스 및 교육 콘텐츠 확대를 제안하였다. 향후, 타 관종의 도서관에서 이루어지는 온라인서비스 현황과 코로나 시대의 도서관 서비스 유형과 사례연구를 통하여 앞으로의 도서관 비대면 서비스 방향을 모색하는 연구가 진행되어야 할 것으로 보인다.

• 한국의류학회지
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• 제29권3_4호
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• pp.403-413
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• 2005

The purpose of this study is to interpret the elegant dressing visualized in modem fashion on the basis of the concept of elegance in dress and its aesthetic characteristics from the holistic viewpoint. Elegance in dress is based upon the idea of aristocratic taste cultivated by good breeding, considering from the documentary study. It is expressed visually through not only the carefully contrived dress but also a sort of aura of dressed body with skillful ease. The aesthetic values of elegance consist of luxury, nobility, refinement, femininity, harmony. To grasp the trends of elegant styles since 1990s, contents analysis of the articles related to elegance in Vogue has been done. As a result, they have been classified into four groups of elegant styles which are Classic, Elaborate Couture, Soft Minimalism, Kitsch Elegance. Classic Elegance and Elaborate Couture Elegance represent traditional ones with conservative viewpoint. Soft Minimalism Elegance is a modem version of elegance. According to postmodernism as a open system, even kitsch has been refined to keep accompany with elegance fur aristocratic taste of high class. As a result of this study, in fashion from the 16th century to the first half of 16th century, elegance has been one of the significant aesthetic categories, resulting from the absolute domination of taste of high society. However in the end of 20th century it seems to start to fade in fashion trends such as mass fashion, youth culture, casual fashion etc. Rather, it can be thought that elegance has been expressed as one of the aesthetic values in dress, by virtue of its value of high class as well as its conservative stability.

• 동서간호학연구지
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• 제3권1호
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• pp.7-16
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• 1998

This paper reviewed the concept of the energy flow in east and west. The differences in the views of the energy flow between oriental and western thought were compaired according to the ontological point of view. Human body take up energy and material on open system which maintains homeostasis. Human and environment are continuously interacting in a state of co-existence. Human has energy field called "aura" and seven chakra in energy outflow moving came as meridian. Qi is an invisible cosmic energy helping the fundamental human activity and changes. The basic action of Qi is helping the human growth and development, maintain the body temperature and preventing the intrusion of microorganism. In the normal healthy organism, all are maintained in balance and in a continuous circulation of Qi, while illness is the result of the disturbed Qi flow in the aspect of Qi-theory. Although there are differences between oriental and western medicine in approaches to clients, the basic point of view and philosophy have many similarities on fundamental level. An understanding of the basic thought of energy flow and oriental concept of energy flow implies a more comprehensive meaning than the perspective of unitary transformation discussed in modern western thought of energy flow. Now we should avoid narrow view of energy concept and regard energy flow as an integrated concept with Korean culture. Regarding Qi-theory which provides a comprehensive and humanistic and ethical foundation for nursing philisophy through this overview, it is hoped that a contribution will be made to the development of nursing intervention which is suitable to Korean context.

• 디지털융복합연구
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• 제10권1호
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• pp.125-133
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• 2012

스마트융합 환경속에서 스마트폰 등의 기기들을 이용한 전자결제서비스가 크게 늘어나게 될 전망이다. 과거 전통적인 전자상거래 방식에서 진일보된 모바일 전자결제 문화가 정착되도록 하는 노력이 필요하다. 비 금융기관들의 진출에 따른 금융기관과의 주도권 갈등 문제를 해결하기 위해 모바일 지급결제 인프라를 표준화하는 노력이 필요하며 NFC 기술 기반의 스마트폰을 통한 거래 증가에 대비한 국내외적 전략이 필요하다. 다양한 유형의 사업자들이 전자상거래 분야로 진출함에 따라 소비자 불만 해소 노력이 중요하며 개방형 운영체제로 전환되는 과정에서의 보안 문제 또한 중요 이슈가 되고 있다. 본 연구에서는 국내외 현황을 조사하고 한국이 처하고 있는 문제점을 분석해내고 그에 대한 정책 대응 방안을 제시하였다. 정부의 전자상거래 정책 마련에 도움이 되기를 기대한다.

• Journal of Microbiology and Biotechnology
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• 제19권3호
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• pp.257-264
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• 2009

A $\beta$-agarase gene, agaB34, was functionally cloned from the genomic DNA of a marine bacterium, Agarivorans albus YKW-34. The open reading frame of agaB34 consisted of 1,362 bp encoding 453 amino acids. The deduced amino acid sequence, consisting of a typical N-terminal signal peptide followed by a catalytic domain of glycoside hydrolase family 16 (GH-16) and a carbohydrate-binding module (CBM), showed 37-86% identity to those of agarases belonging to family GH-16. The recombinant enzyme (rAgaB34) with a molecular mass of 49 kDa was produced extracellularly using Escherichia coli $DH5{\alpha}$ as a host. The purified rAgaB34 was a $\beta$-agarase yielding neoagarotetraose (NA4) as the main product. It acted on neoagarohexaose to produce NA4 and neoagarobiose, but it could not further degrade NA4. The maximal activity of rAgaB34 was observed at $30^{\circ}C$ and pH 7.0. It was stable over pH 5.0-9.0 and at temperatures up to $50^{\circ}C$. Its specific activity and $k_{cat}/K_m$ value for agarose were 242 U/mg and $1.7{\times}10^6/sM$, respectively. The activity of rAgaB34 was not affected by metal ions commonly existing in seawater. It was resistant to chelating reagents (EDTA, EGTA), reducing reagents (DTT, $\beta$-mercaptoethanol), and denaturing reagents (SDS and urea). The E. coli cell harboring the pUC18-derived agarase expression vector was able to efficiently excrete agarase into the culture medium. Hence, this expression system might be used to express secretory proteins.

• 가정과삶의질연구
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• 제28권5호
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• pp.167-181
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• 2010

This research was designed to explore the meaning of housing among Korean Chinese in Harbin, China. In particular, the meaning of housing was examined by using the pathway approach. Utilizing qualitative research methods, this study administered the in-depth interview on the oral history of an individual life, and the 5 elderly persons in their 60s and 70s participated in the individualized interviews that were conducted from May 28 to 31 in 2010. The main findings of meaning of housing were as follows; 1. Similarly to the meaning of housing in 1970s and 1980s in Korea, house was viewed as both a shelter for family members and relatives and a place for their comfort. 2. Prior to multi-story residences, Harbin had only 3 different forms of single-story houses available; Chinese style with Kang and soil room(地室), Korean style with 'Ondol', and Russian style with open floor and Pechka, The promotion at work enabled participants to move to multi-story residences, their moving time varied from 1970 to 1991, and the residential moving determined their current housing status. 3. Multi-story residences were available around 1970s, floor-heating system was introduced from 1990s, and high-rise apartments were built from 1998. Korean Chinese(朝鮮族) weren't satisfied with the spatial composition of individual units embedded into the Chinese culture, especially, entrance, kitchen, bathroom and veranda. 4. Based on assimilation through socialism, adaptation to socialist society and capitalist acculturation, the lifestyles of the interviewees were categorized into five types - capitalist-proactive(Ms. KS), socialist-pragmatic(Ms. J), socialist-inducive(Ms. KY), family centered-conservative(Ms. L), and socialist-adaptive(Ms. P). This study implies that housing-related services for Korean Chinese are necessarily provided so as to embrace their life style and cultural identity in housing design, and further studies need to be explored.

• 복식
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• 제65권4호
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• pp.76-90
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• 2015

Based on a concept of Nietzschean nihilism, this study aimed at interpreting the nihilism in arts and its expressions, also analyzing the modern fashion collection from the same angle. The research was centered on arts after 1980, when post-modern formal destruction expanded in earnest and on the fashion collection after the 20th century, easily accessible to data. Particularly, it set 1994 nihilism collection by Alexander McQueen, a representative nihilist fashion designer as a starting point. Nietzsche mentioned that true arts may be achieved when Apollonian characteristics including a bodily sensory system and an idealization process and Dionysian characteristics including every human feeling are integrated. Besides, he emphasized the importance of an artist being represented as an image of ${\ddot{U}}bermensch$. The ${\ddot{U}}bermensch$ image, reflected in arts and artistic nihilism, represents themes of violence/death, realistic/unrealistic expressions, human body/inhuman aversion materials and the transmutation of a form. Fashion collection expressions, owing to the special characteristic of the show form unlike other arts, were segmented as a realistic ${\ddot{U}}bermensch$ image using a model in a theme, expression, material and form. The theme of violence/death was divided into the death of human and a society. Human life/death was expressed as destruction of human weakness and self-identity, sexual objectification and violence, and social death as destruction and conflict of a class, nation, culture and nature. As for the expression, it was divided into the realistic expression of the primitive/natural and directing of an unrealistic atmosphere using a show.

• The Plant Pathology Journal
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• 제20권1호
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• pp.52-57
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• 2004

Sclerotinia sp. (isolate BWC98-105) causes stem blight and root rot in Leghum sp., and is presently being evaluated as a potential mycoherbicide for the control of Trifoliorium repens. Bioassays have shown that Sclerotinia sp. produces phytotoxic substance which is biologically active against T. repens. Two biologically active compounds, designated as compoundsI and II, were produced in vitro from the culture filtrate of BWC98-105 isolate Sclerotium sp. Compounds I and II were purified by means of liquid-liquid extraction and $C_{18}$ open column chromatography (300 ${\times}$ 30 mm, i.d). To determine the purity, the purified compounds were analyzed by RP-HPLC. The analytical RP-HPLC column was a TOSOH ODS-120T (150 ${\times}$ 4.6 mm i.d, Japan), of which the flow rate was set at 0.7 mL/min using the linear gradient solvent system initiated with 15 % methanol to 85 % methanol for 50 min with monitoring at 254 nm. Under these RP-HPLC conditions, compounds I and II eluted at 3.49 and 4.13 min, respectively. Compound II was found to be most potent and host specific. However, compound I had a unique antibiotic activity against phytopathogenic bacteria like bacterial leaf blight (Xanthomonas oryzae) on rice, where it played a less important role in producing toxicity on T. repens. No toxin activity was detected in the water fraction after partitioning with several organic solvents. However, toxin activity was detected in the ethyl acetate and butanol fractions. In the leaf bioassay using compound II, the disease first appeared within 4-5 h as water soaked rot, which subsequently developed into well-defined blight affecting the whole plant.

• Plant Biotechnology Reports
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• 제2권4호
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• pp.259-265
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• 2008

Picea koraiensis, called Korean spruce, is an evergreen tree and found mostly in northeast Asia. In this study, plant regeneration via somatic embryogenesis from open-pollinated immature zygotic embryos of nine geno-types of elite trees was established. Immature zygotic embryos were cultured onto RJW medium modified from 505 medium with $21.48{\mu}M$ NAA, $2.22{\mu}M$ BA, and $2.32{\mu}M$ KT. The average frequency for all nine genotypes was 74.2%. Embryogenic calluses of the nine genotypes of elite trees were subcultured on RJW basal medium containing $8.06{\mu}M$ NAA, $1.11{\mu}M$ BA, and $1.16{\mu}M$ kinetin. The calluses of three lines, $3^{\sharp}$, $9^{\sharp}$, and $2^{\sharp}$, were actively proliferated but others were not. Somatic embryogenesis was induced from the embryogenic callus in genotypes of $3^{\sharp}$, $9^{\sharp}$, and $2^{\sharp}$ on RJW medium with ABA and $60g\;l^{-1}$ sucrose. Cotyledonary somatic embryos were subjected to a drying process. The drying of embryos by uncapping the culture bottle for 5 days on a clean bench resulted in a high frequency of germination of somatic embryos (87% in RJW medium). However, plantlet conversion from germinated embryos was greatly reduced and the optimal medium for plant conversion was 1/2 WPM or 1/2 BMI medium. In conclusion, we have, for the first time, established a plant regeneration system via somatic embryogenesis in the Korean spruce, which can be applied for rapid micropropagation of elite trees.