• 한국언론정보학보
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• 제66권
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• pp.5-27
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• 2014

우리나라 온라인 저널리즘 지형의 과거, 현재, 미래는 무엇보다 포털 뉴스서비스와 밀접한 연관이 있다. 전통 저널리즘에서 한정된 뉴스미디어는 뉴스 생산과 유통을 동시에 수행하기 때문에 소비를 완전히 장악할 수 있었다. 이후 다매체다채널 시대에는 뉴스미디어가 큰 폭으로 증가하고 이에 따라 뉴스 생산이 폭발적으로 늘어나면서 수용자의 선택이 중요하게 됐다. 온라인 저널리즘 환경에서는 각종 뉴스 이용을 간편하게 만든 포털 뉴스서비스에 대한 이용의 집중, 뉴스미디어 브랜드보다는 뉴스 콘텐츠에 집중하는 뉴스 소비의 특성 등으로 인해 뉴스 유통이 가장 중요한 영역으로 부각됐다. 포털 뉴스서비스가 온라인 저널리즘의 상징적 존재가 되면서 뉴스 유통 중심의 우리나라 저널리즘 지형이 완성된 것이다. 뉴스 유통만을 담당하는 포털 뉴스서비스가 뉴스를 생산하는 언론사닷컴이나 인터넷신문보다 더 큰 영향력을 가진다는 것은 언론 권력의 변화를 의미한다. 이로 인해 기존 뉴스미디어와 포털 뉴스서비스의 갈등이 곳곳에서 목격된다.

• 한국건축시공학회:학술대회논문집
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• 한국건축시공학회 2012년도 추계 학술논문 발표대회
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• pp.279-280
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• 2012

The Construction Standard Production Unit(CSPU) has been used for the standard cost estimate in public and private construction projects. However, It is questionable if the reliability and/or authenticity of the system is acceptable due to the lack of consistent enactment and/or revision procedures. This study identifies informational conditions and issues relative to using daily work report as a data collection method for enacting and/or revising CSPU system, and establishes the measures for improving the daily work report. This study aims on the information constitution of daily job-site report, daily manpower report, wages register for specialty contractors. According to the research results, most of necessary data were included in a daily job-site report. In conclusion, it is investigated that data of daily manpower report and wage register need to be included in a daily job-site report for understanding the current state of worker in the future.

• 상하수도학회지
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• 제29권5호
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• pp.575-581
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• 2015

Although disinfection in drinking water treatment plants provides a safer water supply by inactivating pathogenic microorganisms, harmful disinfection by-products may be formed. In this study, the disinfectant, chlorine, was produced on-site from the electrolysis of salt (NaCl), and the by-products of the disinfection process, bromate and chlorate, were analyzed. The provisional guideline levels for bromate and chlorate in drinking water are $10{\mu}g/L$ and $700{\mu}g/L$, in Korea, respectively. Bromide salt was detected at concentrations ranging from 6.0 ~ 622 mg/kg. Bromate and chlorate were detected at concentrations ranging from non-detect (ND) ~ 45.3mg/L and 40.5 ~ 1,202 mg/L, respectively. When comparing the bromide concentration in the salt to the bromate concentration in the chlorine produced by salt electrolysis, the correlation of bromide to bromate concentration was 0.870 (active chlorine concentration from on-site production: 0.6-0.8%, n=40). The correlation of bromate concentration in the chlorine produced to that in the treated water was 0.866.

• Archives of Pharmacal Research
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• 제28권3호
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• pp.274-279
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• 2005

Pharmacological inhibition of interleukin-12 (IL-12) production may be a therapeutic strategy for preventing the development and progression of disease in experimental models of autoimmunity. In this study, the effects of myricetin, a naturally occurring flavonoid present in fruits, vegetables and medicinal herbs, on the production of IL-12 were investigated in mouse macrophages stimulated with lipopolysaccharide (LPS). Myricetin significantly inhibited the LPS­induced IL-12 production from both primary macrophages and the RAW264.7 monocytic cell-line in a dose-dependent manner. The effect of myricetin on IL-12 gene promoter activation was analyzed by transfecting RAW264.7 cells with IL-12 gene promoter/luciferase constructs. The repressive effect was mapped to a region in the IL-12 gene promoter containing a binding site for NF-${\kappa}B$. Furthermore, activation of macrophages by LPS resulted in markedly enhanced binding activity to the NF-${\kappa}B$ site, which significantly decreased upon addition of myricetin, indicating that myricetin inhibited IL-12 production in LPS-activated macrophages via the down­regulation of NF-KB binding activity.

• Journal of Microbiology and Biotechnology
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• 제33권3호
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• pp.410-418
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• 2023

Bacilysin is a dipeptide antibiotic composed of L-alanine and L-anticapsin produced by certain strains of Bacillus subtilis. Bacilysin is gaining increasing attention in industrial agriculture and pharmaceutical industries due to its potent antagonistic effects on various bacterial, fungal, and algal pathogens. However, its use in industrial applications is hindered by its low production in the native producer. The biosynthesis of bacilysin is mainly based on the bacABCDEF operon. Examination of the sequence surrounding the upstream of the bac operon did not reveal a clear, strong ribosome binding site (RBS). Therefore, in this study, we aimed to investigate the impact of RBS as a potential route to improve bacilysin production. For this, the 5' untranslated region (5'UTR) of the bac operon was edited using the CRISPR/Cas9 approach by introducing a strong ribosome binding sequence carrying the canonical Shine-Dalgarno sequence (TAAGGAGG) with an 8 nt spacing from the AUG start codon. Strong RBS substitution resulted in a 2.87-fold increase in bacilysin production without affecting growth. Strong RBS substitution also improved the mRNA stability of the bac operon. All these data revealed that extensive RBS engineering is a promising key option for enhancing bacilysin production in its native producers.

• Preventive Nutrition and Food Science
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• 제6권1호
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• pp.79-81
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• 2001

Deoxynucleoside kinases exist as heterodimeric pairs specific for deoxyadenosine/deoxyguanosine kinase (dAK/dGK) and deoxyadenosine/deoxycytidine kinase (dAK/dCK). The aspartic acid-84 in dGK was mutated to alanine, asparagine and glutamic acid by site-directed mutagenesis. The mutation resulted in a drastic decease in dGK activity compared to the unmodified cloned enzyme while it increased production of dAK activity. The mutated dak/dgk genes, which synthesize tandem deoxyadenosine/deoxyguanosine kinase, were inserted back to the Lactobacillus acidophilus and Lactococcus lactis by electroporation to determine the effect of site-directed mutation of he enzymes on the microbial growth. However, no significant change was observed in cell growth and lactic acid production between wild type and mutant lactic acid bacteria.

• 에너지공학
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• 제23권3호
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• pp.102-115
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• 2014

국내 동해 울릉분지에는 막대한 부존량과 청정 에너지원으로 높은 잠재력을 지닌 가스하이드레이트가 부존되어 있으며, 2010년 울릉분지 2차 시추지역(UBGH2)에서 현장 생산 시험 후보지 선정을 위한 목적으로 시추작업이 수행되었다. 이 연구에서는 동해 울릉분지 현장 생산 시험 후보지역의 지질학적 모델을 바탕으로 전산 수치 해석을 이용하여 가스하이드레이트 퇴적층 내 해리 유동 및 산출성을 분석하였으며, 주요 생산 조건별 감압 생산 거동에 대한 민감도 분석을 수행하였다. 이를 통해 가스하이드레이트 현장 생산 시험 계획 수립 시 요구되는 예비 분석 자료를 도출하였다.

• Advances in environmental research
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• 제3권2호
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• pp.173-183
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• 2014

A comprehensive mathematical model was developed for this study to estimate on-site and off-site GHG emissions from wastewater treatment plants (WWTPs). The model was applied to three different hybrid WWTPs (S-WWTP, J-WWTP, and T-WWTP) including anaerobic, anoxic, and aerobic process, located in Seoul City, South Korea. Overall on-site and off-site GHG emissions from S-WWTP, J-WWTP, and T-WWTP were $305,253kgCO_2e/d$, $282,682kgCO_2e/d$, and $117,942kgCO_2e/d$, respectively. WWTP treating higher amounts of wastewater produced more on-site and off-site GHG emissions. On average, the percentage contribution of on-site and off-site emissions was 3.03% and 96.97%. The highest amount of on-site GHG emissions was generated from anoxic process and the primary on-site GHG was nitrous oxide ($N_2O$). Off-site GHG emissions related to electricity consumption for unit operation was much higher than that related to production of chemicals for on-site usage. Recovery and reuse of biogas significantly reduced the total GHG emissions from WWTPs. The results obtained from this study can provide basic knowledge to understand the source and amount of GHG emissions from WWTPs and strategies to establish lower GHG emitting WWTPs.

• 한국건설관리학회논문집
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• 제5권1호
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• pp.114-123
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• 2004

Contract time determination for highway construction projects has never been easy despite considerable research efforts from academia as well as industry. High variations in crew production rates are considered one of the main barriers to accurate contract time determination. This paper presents a methodology for collecting field information on crew production rates which will help to enhance the accuracy of contract time determination for highway bridge construction. Based on a standard data collection tool developed, data on field crew production rates was collected from 14 on going projects in Texas, USA, over the past two years. The production rates based on the data collected were considered by industry practitioners to be more realistic and practical than those available to the current practices. As more data becomes available, key drivers influencing production rates could be identified and provide site personnel with a means to better plan and control production in a project specific context.

• 한국태양에너지학회:학술대회논문집
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• 한국태양에너지학회 2008년도 추계학술발표대회 논문집
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• pp.300-305
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• 2008

Using WindPRO that was software for windfarm design developed by EMD from Denmark, wind resources for the western Jeju island were analyzed, and the performance of WindPRO prediction was evaluated in detail. The Hansu site and the Yongdang site that were located in coastal region were selected, and wind data for one year at the two sites were analyzed using WindPRO. As a result, the relative error of the Prediction for annual energy Production and capacity factor was about ${\pm}20%$. For evaluating wind energy more accurately, it is necessary to obtain lots of wind data and real electric power production data from real windfarm.