• Journal of Life Science
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• 제24권12호
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• pp.1301-1307
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• 2014

Adipose-derived stem cells (ADSCs) are considered promising tools for tissue regeneration. However, ADSCs have very poor proliferation capacity. Therefore, fetal bovine serum (FBS) is generally added to the culture media of ADSCs. As FBS contains many uncharacterized components that may affect cellular functions, methods for serum-free cultures of ADSCs have been widely investigated. In this study, to develop an efficient method for a serum-free culture of ADSC-T, we used an ADSC line established by introducing the simian virus 40 (SV40) T gene into primary ADSCs. We then investigated the effect of amino acids, vitamins, and other components on the growth of ADSC-T. When the ADSC-T cells were plated with DMEM/F12 serum-free medium, the cells did not proliferate, and the mixture of amino acids, vitamins, and B27 supplement did not increase the growth of the cells. However, when the ADSC-T cells were provided with serum-free DMEM/F12 after they had been cultured with serum-supplemented DMEM for 24 h, the cells proliferated, and the vitamins and B27 supplement increased the cell growth. Stem-Pro serum-free medium also appeared to be useful as a suspension culture for the ADSC-T cells. The ADSC-T cells secreted large amounts of proteins of around 70 kDa. Insulin-like growth factor (IGF) and fibroblast growth factor basic (FGF basic) were secreted by ADSC-T in larger amounts in the serum-free culture than in the serum-supplemented culture.

• Journal of Broadcast Engineering
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• 제18권6호
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• pp.859-871
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• 2013

In this paper, we propose an algorithm that generates multi-view images by grid-mesh based image domain warping using occlusion mask and various image features obtained from the stereoscopic images. In the proposed algorithm, we first extract image saliency map, line segments and disparity saliency map from stereo images and then get them through a process that improves the quality of extracted features. This process is accomplished in two steps. In the first step, reliability of disparity saliency map on object boundary regions is enhanced by using occlusion information. And in the second step, we enhance the quality of image features in terms of temporal consistency by using temporal consistency information for stereo images. With these enhanced features, multi-view images are generated by grid-mesh based image domain warping technique. Experimental results show that the proposed algorithm performs better than existing algorithms in terms of visual quality.

• The Proceedings of the Korean Institute of Illuminating and Electrical Installation Engineers
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• 제7권3호
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• pp.49-56
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• 1993

This paper describes design and implementation of signal transmission system using LCX as communication media, which has characteristics of high reliability easy for expansion and complex transmission of voice, data and video signal in Electric culvert. In this system, we estimated system performance as result of variable transmission characteristics test. In case of voice signal, transmission loss characteristics improved 5-10(dB] than designed Values in received signal level. In the test of speech quality estimation, we obtained satisfactory result as speech intensity = 3 (QSA value), speech atriculation = 4 (QRK value). As for data and video signal transmission, communication success rates were 981% 1 in monitoring and control functional test. As a result of transmission characteristics test in transmission line and system, transmission range by LCX communication system without repeater can reach in 6Km. This paper presents basic construction method using LCX communication system for total management in Electric culvert.

• The Korean Journal of Pain
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• 제25권3호
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• pp.168-172
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• 2012

Background: The aim of the study was to investigate the feasibility of fluoroscopy-guided anterior approach for suprascapular nerve block (SSNB). Methods: Twenty patients with chronic shoulder pain were included in the study. All of the nerve blocks were performed with patients in a supine position. Fluoroscopy was tilted medially to obtain the best view of the scapular notch (medial angle) and caudally to put the base of coracoid process and scapular spine on same line (caudal angle). SSNB was performed by introducing a 100-mm, 21-gauge needle to the scapular notch with tunnel view technique. Following negative aspiration, 1.0 ml of contrast was injected to confirm the scapular notch, and 1 % mepivacaine 2 ml was slowly injected. The success of SSNB was assessed by numerical rating scale (NRS) before and after the block. Results: The average NRS was decreased from $4.8{\pm}0.6$ to $0.6{\pm}0.5$ after the procedure (P < 0.05). The best view of the scapular notch was obtained in a medial angle of $15.1{\pm}2.2$ ($11-19^{\circ}$) and a caudal angle of $15.4{\pm}1.7^{\circ}$ ($12-18^{\circ}$). The average distance from the skin to the scapular notch was $5.8{\pm}0.6$ cm. None of the complications such as pneumothorax, intravascular injection, and hematoma formation was found except one case of partial brachial plexus block. Conclusions: SSNB by fluoroscopy-guided anterior approach is a feasible technique. The advantage of using a fluoroscopy resulted in an effective block with a small dose of local anesthetics by an accurate placement of a tip of needle in the scapular notch while avoiding pneumothorax.

• KSBB Journal
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• 제11권2호
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• pp.254-261
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• 1996

Attenuated varicella-zoster virus (VZV) was propagated in human lung fibroblast (HLF) cells Among media tested in this work, DMEM was the best medium for the growth of HLF cells. Because HLF was a normal finite cell line, cell growth late was dependent on the age of HLF cells. When the population doubling level (PDL) was higher than 46, apoptosis of HLF cells started and cell growth rate decreased. The optimum temperature for the cell growth and virus propagation in the T-flask culture was $37^{\circ}C$. In a microcarrier culture system in which Cytodex-3 was used for the VZV propagation in spinner vessels, the yield of plaque forming cells was lower than that in the T-flask culture. The relatively high shear environment near microcarriers was thought to cause the low yield of VZV in the microcarrier culture system.

• Journal of Broadcast Engineering
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• 제18권3호
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• pp.380-392
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• 2013

A method to classify a golf swing motion into 7 sections using a Kinect sensor and a fuzzy system is proposed. The inputs to the fuzzy logic are the positions of golf club and its head, which are extracted from the information of golfer's joint position and color information obtained by a Kinect sensor. The proposed method consists of three modules: one for extracting the joint's information, another for detecting and tracking of a golf club, and the other for classifying golf swing motions. The first module extracts the hand's position among the joint information provided by a Kinect sensor. The second module detects the golf club as well as its head with the Hough line transform based on the hand's coordinate. Using a fuzzy logic as a classification engine reduces recognition errors and, consequently, improves the performance of robust classification. From the experiments of real-time video clips, the proposed method shows the reliability of classification by 85.2%.

• Journal of Korea Society of Industrial Information Systems
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• 제10권3호
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• pp.15-20
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• 2005

Since the invention of Web, it became part of our daily life replacing the routine information search and effecting many activities which otherwise could have been done off-line without it. It was a natural evolution of the web technology, which had started out as a simple test based pattern matching, to be based on the optimized match process for its multi-media Web environment, like still images, music and movies that we are to face today. In this paper, we proposed and implemented the model which the multimedia resources can be efficiently shared in semantic web. After converting multimedia resource information(metadata) into RDF, for efficient management, the model separate and allotment actual multimedia resource and corresponding metadata changed to RDF to each server. The proposed model could be applied in all multimedia resources. For easy explanation and implementation, however, we applied it to the digital photograph in example.

• Journal of Korea Multimedia Society
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• 제12권9호
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• pp.1342-1350
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• 2009

Until today, the uses of digital display system mostly included demonstration, observation, and exhibition of simple images. The strengths were that the images that changes in line with the music played could be reinterpreted as a different work. Also, it could stimulate the sensibility of the users. However, the range of the uses of the system is very limited and users could easily lose their interest. Therefore, it is necessary to develop a system that could continuously induce and motivate user interaction. Therefore, the RSS based digital folding screen system proposed by this paper has an objective of being used as an interactive media system which overcome the limitations of former eight-sided folding screen used for simple observation purposes. It also applied the main function of RSS in which the user could collect and read newly registered messages without visiting the blogs or corresponding websites. Therefore, it is capable of sending the information needed by the user. Generally, we can observe the contents for mere observation purposes and when necessary, it could also be utilized for advertisement or exhibition purposes. It could also be used as a information transfer system in public facilities.

• Journal of Broadcast Engineering
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• 제11권2호
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• pp.174-180
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• 2006

H.264/MPEG-4 AVC is jointly developed by ITU-T and ISO/IEC. It provides efficient coding efficiency compared with previous video standards. It reduced the bit rate by approximately $30%{\sim}70%$ while providing the same or better image quality. And, H.264/MPEG-4 AVC supports not only lossy coding but also lossless coding. In this paper, we suggest a method to improve lossless coding efficiency. Proposed method is based on Intra residual DPCM, it has same effect with the prediction from spatially nearest pixel. Also, proposed method does not broken decoder pipe-line. Experimental results, the method reduced the bit rate by approximately 12% in comparison with the H.264 Intra lossless coding. As a result, it is adopted into the H.264/MPEG-4 AVC Advanced 4:4:4 profile.

• Journal of Nutrition and Health
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• 제36권3호
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• pp.270-279
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• 2003

Conjugated linoleic acid (CLA) is a group of positional and geometric isomers of linoleic acid (LA) and exhibits anticarcinogenic activity in a variety of animal models. We have previously observed that CLA inhibited the growth of Caco-2 cells, a human colon adenocarcinoma cell line. The present study was performed to determine whether the growth inhibitory effect of CLA is related to change in secretion of IGF- II and/or IGF-binding proteins (IGFBPs) that have been shown to regulate Caco-2 cell proliferation by an autocrine mechanism. Cells were incubated in serum-free medium with various concentrations of CLA or linoleic acid (LA). Immunoblot analysis of 24-hours, serum-free, conditioned medium using a monoclonal anti-IGF-IIantibody revealed that Caco-2 cells secreted both mature 6,500 Mr and higher Mr forms of pro IGF-II. The levels of pro IGF-II and mature IGF-IIwere decreased by 43 $\pm$ 2% and 53 $\pm$ 6%, respectively by treatment with 50 $\mu$ M CLA. LA slightly increased pro IGF- II levels. Results from Northern blot analysis showed that CLA decreased IGF-II mRNA levels at 50 $\mu$ M concentration suggesting that CLA regulation of IGF-II protein expression occurs partly at the transcriptional level. Ligand blot analysis of conditioned media using 1251-IGF-II revealed that CLA slightly decreased IGFBP-2 levels and increased IGFBP-4 levels. We confirmed our previous results that CLA inhibited cell growth in a dose-dependent manner but LA slightly increased cell growth. Exogenous IGF-II mitigated the growth inhibitory effect of CLA. These results indicate that the growth inhibitory effect of CLA may be at least in part mediated by decreasing IGF-II and IGFBP-2 secretion and increasing IGFBP-4 secretion in Caco-2 cells.