• Journal of Life Science
• /
• v.27 no.4
• /
• pp.417-422
• /
• 2017

The purpose of this study was to investigate the role of the Vaccinum oldhami fruit extract as a cosmetic additive. As a result of having macrophage (RAW 264.7) measured a cell toxicity effects of 70% ethanol extract from Vaccinum oldhami fruit, it shown 118% with toxicity at $500{\mu}g/ml$ concentration. In nitric oxide synthesis inhibition effect, 70% ethanol extracts from Vaccinum oldhami fruit shown 47.3% at $1,000{\mu}g/ml$ concentration. The iNOS, COX-2 protein expression inhibitory effect by western blot of 70% ethanol extract from Vaccinum oldhami fruit was decreased by 36.13%, 29.61% at $500{\mu}g/ml$ concentration. And iNOS, COX-2 mRNA expression inhibitory effect by reverse-transcription-PCR of 70% ethanol extract from Vaccinum oldhami fruit was decreased by 62.25%, 90.07% at $500{\mu}g/ml$ concentration. All these finding that extract from Vaccinum oldhami fruit could prove that their have effects anti-inflammatory efficacy. And extract from Vaccinum oldhami fruit has potential as a cosmetic ingredients.

• Korean Journal of Pharmacognosy
• /
• v.41 no.3
• /
• pp.180-184
• /
• 2010

The antioxidant activity of Desmodium oldhami (Leguminosae) was determined by measuring the radical scavenging effect on DPPH (1,1-diphenyl-2-picrylhydrazyl). The methanolic extract of D. oldhami showed the strong antioxidant activity. Five compounds, kaempferol-3-O-rhamnopyranoside (afzeline) (1), quercetin-3-O-[$\alpha$-L-rhamnopyranosyl($1{\rightarrow}6$)-$\beta$-Dglucopyranoside] (rutin) (2), kaempferol-3-O-glucopyranoside (astragalin) (3), genistein-7-O-$\beta$-D-glucopyranoside (genistin) (4), kaempferol-3-O-rutinoside (5) were isolated from the active ethyl acetate soluble fraction of D. oldhami through repeated silica gel and Sephadex LH-20 column chromatography. Among them, compound 2 showed the most significant antioxidative effect on DPPH free radical scavenging test. Compounds 1-5 are reported for the first time from this plant.

• Korean Journal of Pharmacognosy
• /
• v.36 no.3 s.142
• /
• pp.191-194
• /
• 2005

Four compounds were isolated from the twigs of Vaccinium oldhami (Ericaceae) through repeated column chromatography. Their chemical structures were elucidated as (+)-catechin, (-)-epicatechin, proanthocyanidin A-2, and cinnamtannin $B_1$, respectively, by spectroscopic analysis.

• Korean Journal of Plant Resources
• /
• v.25 no.1
• /
• pp.72-79
• /
• 2012

Vaccinium oldhami Miq. is a Korean native tree, which is deciduous and shrub tree with broad leaf. It grows 1~4m in height generally. Ecologically, this tree grows well in shady place even in barren soil. Also, the tree has resistance to cold and dry, which tend to form a little community. This research investigates quantitative morphological characteristics of leaf and fruit among the V. oldhami in South Korea and then considers its relationship on the basis of raw data among the 10 populations. This study will give us invaluable information about growing conditions, reasonable management and breeding by selection of V. oldhami in South Korea. The main results obtained from this study are summarized as follows; Leaf size of Mudeung population was larger than other populations. Naebyeon population was smaller in size of the leaf than other populations. Anmyeondo population was larger in fruit characteristics compared with other populations and Deogyu population was the smallest among populations. According to cluster analysis based on the leaf and fruit morphological characteristics, the natural V. oldhami populations were classified into four groups such as the first group of Kumo population, the second group of Mudeung population, the third group of Anmyundo, Daedun, Doolyun population and the fourth group of the other five populations.

• Journal of Life Science
• /
• v.20 no.8
• /
• pp.1235-1240
• /
• 2010

Natural compounds have been studied to substitute synthetic antioxidants. In this study, the anti-oxidant activity of 70% acetone extracts from the Vaccinium oldhami fruit was investigated for utilization as ingredients for the cosmetic and bio-industries. Anti-oxidant activity was determined by determining total polyphenolic content, electron donating ability, nitric oxide (NO) radical scavenging activity, $ABTS{\cdot}^+$ cation radical scavenging activity and hydrogen peroxide scavenging activity. The polyphenolic content of 70% acetone extracts of the Vaccinium oldhami fruit was 55.972 mg TAE/g. In electron donating activity, 70% acetone extracts of the Vaccinium oldhami fruit showed an effect of 93.9%, which was similar to BHA effect at a concentration of 500 ${\mu}g/ml$. In the NO radical scavenging ability, 70% acetone extracts of the Vaccinium oldhami fruit showed 60% at 500 ${\mu}g/ml$. $ABTS{\cdot}^+$ cation radical scavenging activity of the Vaccinium oldhami fruit at a concentration of 1000 ${\mu}g/ml$ was 75.7%. Also, hydrogen peroxide scavenging activity of 70% acetone extracts showed 80.8% at 100 ${\mu}g/ml$, whichwas higher than BHA. In the natural compound market, the most important factors are the ability to obtain high effects of a material in low concentrations and a long-lasting supply. The Vaccinium oldhami fruit can be harvested every year - this fulfills one of the requirements. From these results, we can confirm that the Vaccinium oldhami fruit has anti-oxidant abilities and has potential as a natural anti-oxidant agent to be utilized in the cosmeceutical and bio-industries.

• Archives of Pharmacal Research
• /
• v.27 no.1
• /
• pp.53-56
• /
• 2004

In the course of finding Korean natural products with acetylcholinesterase (AChE) inhibitory activity, we found that a methanolic extract of the twigs of Vaccinium oldhami significantly inhibited AChE. Bioassay-guided fractionation of the methanolic extract resulted in the isolation of two compounds, taraxerol (1) and scopoletin (2), as active constituents. These compounds inhibited AChE activity in a dose-dependent manner, and the $IC_{50}$/ values of compounds 1 and 2 were 33.6 (79 $\muM$) and 10.0 (52 $\muM$) $\mu$g/mL, respectively.

• Korean Journal of Plant Resources
• /
• v.18 no.2
• /
• pp.260-269
• /
• 2005

Taxonomic studies on morphological, principal component analysis (PCA), palynological, RAPD and PCR-RELP analysis were conducted to intraspecific relationships of Rubus oldhami. Three types of Rubus oldhami based on the flower characters such as petal length and number were used in this study. Among the 14 morphological characters, perianth length, calyx lobe length, apical leaflet shape and leaflet length were used to distinguish for each type. The pollen characters such as shape, aperture number, surface sculpture were showed very similar among three types. Eight primers out of 20 arbitrary primers were screened for three types, and were revealed 33 ($60\%$) polymorphic bands. The phonogram by RAPD data showed incongruent with morphological analysis. Even though ten restriction endonucleases produced 20 restriction sites, polymorphic bands were not observed. Based on the results, three types of Rubus oldhami divided well by morphological characters, but pollen and DNA data were not supported. Therefore, type 1 and 2 which different from type 3 by flower characters considered as a temporary hybrid or ecotype because of their similar habitats.

• Journal of the Korean Wood Science and Technology
• /
• v.32 no.5
• /
• pp.20-28
• /
• 2004

26 wooden relics excavated in Jedeok bay, Jinhae, Gyeongsangnam-do, Korea were identified. Wood species identified were consisted of 7 softwoods and 19 hardwoods. Softwoods identified were hard pines (Pinus spp.), while 19 hardwoods were consisted of 15 Lepidobalanus (Quercus spp.), 1 Cyclobalanopsis (Quercus spp.), 1 Meliosma oldhami Miq., 1 Platycarya strobilacea S. et Z., and 1 Carpinus spp., respectively. The wooden fences were composed of a variety of wood species such as hard pines (Pinus spp.), Lepidobalanus (Quercus spp.), Meliosma oldhami Miq. and Carpinus spp. Wooden members of ship were consisted of Lepidobalanus (Quercus spp.), and parts of ship body were hard pines(Pinus spp.). The other relics that uses were unknown were hard pines (Pinus spp.), Lepidobalanus (Quercus spp,), Cyclobalanopsis (Quercus spp.), and Platycarya strobilacea S. et Z.

• Korean Journal of Pharmacognosy
• /
• v.38 no.3 s.150
• /
• pp.296-298
• /
• 2007

Two lignan glycoside compounds were isolated from the twigs of Vaccinium oldhami (Ericaceae) through repeated column chromatography. Their chemical structures were elucidated as lyoniside and ssioriside, respectively, by spectroscopic analysis.

• Journal of Life Science
• /
• v.33 no.2
• /
• pp.115-128
• /
• 2023

The fruit of Vaccinum oldhami was separated and purified to obtain the compounds chlorogenic acid (CA), quercetin (QT), and quercitrin (QR). The electron-donating abilities of CA, QT, and QR at 1,000 ㎍/ml were 91.9%, 89.9%, and 77.4%, respectively QT and QR showed 99.5% and 91.4% ABTS⁺ radical scavenging ability at a 1,000 ㎍/ml concentration, respectively, and CA showed a 95% ability or higher at 100 ㎍/ml. Regarding tyrosinase inhibitory activity, CA, QT, and QR exhibited 29.5%, 34.7%, and 23.7% efficacy, respectively, at 1,000 ㎍/ml. Regarding the cell viability for melanoma cells (B16F10) assessed through MTT assay, CA, QT, and QR showed cell a viability of 80% or more at 100 ㎍/ml. To measure the deterrent of protein expression, CA affected TRP-1 and TRP-2 in accordance with increases in concentration. The protein expression inhibition rate of QT was excellent for TRP-1, TRP-2, and tyrosinase. CA was confirmed to have an excellent mRNA expression inhibitory effect against MITF, and the amount of mRNA expression of TRP-1, TRP-2, and tyrosinase decreased with an increase in the CA concentration. As the concentration of QT increased, the mRNA expression of MITF, TRP-2, and tyrosinase decreased. QR decreased the amount of mRNA as the QR concentration increased. The excellent antioxidant and whitening effects of CA, QT, and QR were thus confirmed.