• BMB Reports
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• v.40 no.4
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• pp.501-510
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• 2007

Sex-related genes expressed in vitellogenic ovaries of the giant tiger shrimp, Penaeus monodon, were identified by an EST approach. A total of 1051 clones were unidirectionally sequenced from the 5 terminus. Nucleotide sequences of 743 EST (70.7%) significantly matched known genes previously deposited in the GenBank (E-value <$10^{-4}$) whereas 308 ESTs (29.3%) were regarded as newly unidentified transcripts (E-value >$10^{-4}$). A total of 559 transcripts (87 contigs and 472 singletons) were obtained. Thrombospondin (TSP) and peritrophin (79 and 87 clones accounting for 7.5 and 8.3% of clones sequenced, respectively) predominated among characterized transcripts. everal full length transcripts (e.g. cyclophilin, profillin and thioredoxin peroxidase) were also isolated. A gene homologue encoding chromobox protein (PMCBX, ORF of 567 nucleotides encoding a protein of 188 amino acids) which is recognized as a new member of the HP1 family was identified. Expression patterns of 14 of 25 sex-related gene homologues in ovaries and testes of P. monodon broodstock were examined by RT-PCR. Female sterile and ovarian lipoprotein receptor homologues were only expressed in ovaries whereas the remaining transcripts except disulfide isomerase related P5 precursor and adenine nucleotide translocator 2 were higher expressed in ovaries than testes of P. monodon broodstock. A homologue of ubiquitin specific proteinase 9, X chromosome (Usp9X) revealed a preferential expression level in ovaries than testes of broodstock-sized P. monodon (N = 13 and 11, P<0.05) but was only expressed in ovaries of 4-month-old shrimp (N = 5 for each sex).

• Journal of Engineering Education Research
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• v.9 no.3
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• pp.78-89
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• 2006

The economic depression during the last decade of the 20th century caused various drastic changes in the social structure, including employment and educational systems. Japanese universities have devoted much effort to research activity and the development of teaching methodology for engineering education was not a major effort for professors. Decrease of 18 years old population, however, forced universities to change this attitude. University education is no more for only elite but is becoming very popular among young people. Another impact came from the 'globalization'of industrial societies such as establishment of WTO and APEC. Japan Accreditation Board for Engineering Education (JABEE) was established to cope with both domestic and international problems in 1999, and JABEE was admitted as a full signatory member of the Washington Accord in 2005. The Professional Engineer Law was amended linked with JABEE. The importance of engineering ethics has been recognized with these social structure changes. Japanese Society for Engineering Education (JSEE) is contributing for reformation of Japanese engineering education through its activities including supporting JABEE. This paper describes the problems now Japanese engineering education is facing and the role of JSEE in order to reorganize the engineering education in Japan.

• BMB Reports
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• v.37 no.3
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• pp.269-274
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• 2004

DNA polymerases without the 3' exonuclease function ($exo^-$ pol) have been widely used in sequencing and SNP genotyping. As a major player that expedited the coming of the postgenomic era, $exo^-$ polymerases worked remarkably well in the Human Genome Sequencing Project. However, it has become a challenge for this class of polymerases to efficiently screen the large number of SNPs that are found in the human genome. For more than three decades it has been recognized that polymerase fidelity varied according to the presence of proofreading activity that is mediated by its internal 3' exonuclease. Polymerases with proofreading function are famous for their high fidelity in DNA replication both in vivo and in vitro, but this well-known class of polymerases has been almost completely neglected in genetic analysis in the postgenomic era. We speculate that $exo^+$ polymerases may exhibit higher nucleotide identification ability when compared to $exo^-$ polymerases for an in vitro genetic analysis. With the application of $exo^+$ polymerases in SNP assays, a novel mechanism for the maintenance of DNA replication, the on/off switch, was discovered. Two new SNP assays have been developed to carry out genome-wide genotyping, taking advantage of the enzymatic properties of $exo^+$ polymerases. Furthermore, the on/off switch mechanism embodies a powerful nucleotide identification ability, which can be used to discriminate the bases that are upstream of the 3' terminus, and thus defines a new concept in de novo sequencing technology. Application of $exo^+$ polymerases to genetic analysis, and especially SNP assays, will greatly accelerate the pace to personalized medicine.

• Pediatric Infection and Vaccine
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• v.19 no.3
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• pp.157-161
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• 2012

Streptococcus anginosus is a member of Streptococcus milleri group, and is found in the oral mucosa, respiratory tract, and gastrointestinal tract as normal flora. It can develop into a disease in patients with deteriorating clinical condition or with clinical risk factors. A previously healthy 15-year-old boy was admitted due to fever, abdominal discomfort and vomiting which lasted for 7 days. He had a history of dental procedure 1 day before the development of fever. He was diagnosed with acute acalculous cholecystitis based on the clinical, laboratory, and imaging finding, and S. anginosus was isolated from the blood culture. The patient was successfully treated with antibiotic therapy.

• Communications of Mathematical Education
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• v.25 no.1
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• pp.1-19
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• 2011

HPM(History and Pedagogy of Mathematics) become an important issue to us now. Study on old Korean mathematics books were made recently. We study mathematics books in Kyujanggak in this article. Horng Wann-Sheng 洪萬生, an math. historian and a member of editorial board of Historia Mathematica, visited Kyujanggak, the royal library of Joseon Dynasty. After his visit, he published a paper, "The first visit to mathematics books in Kyujanggak 奎章閣收藏算書初訪"(2008 Kyujanggak 32, p. 283-293). In his paper, he also raised several research problems on the history of Korean mathematics. In this paper, we analyze his paper "The first visit to mathematics books in Kyujanggak" and give some answers to those raised problems on Korean mathematics. Also we correct some misunderstanding of Horng on some facts. Especially, we make it clear that the author of SinJungSanSul(New Arithmetics 新訂算術) was not Lee Sang-Seol(李相卨), whom Horng considered as the author, but Lee Gyo-Seung(李敎承) through the correct translation of its preface and an article about its copyright lawsuit. And we added some pathways how Chinese mathematics books were imported by Joseon. We introduce the case of Hong Dae-Yong(洪大容) in detail.

• Journal of the Korea Fashion and Costume Design Association
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• v.8 no.3
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• pp.59-71
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• 2006

This study contemplates Croatian traditional costumes with focus on its geographical characteristics and acculturation with neighbor countries as a member of old Yugoslavian Union. Therefore, it contains the information from field works based on Croatian language, native costumes and folk materials wildly. Men usually wear wide linen pants ($ga\'{c}$), shirt ($ko\v{s}ulje,\;rnbina)$, vest (lajbek), hat and jacket ($kro\v{z}et$). Also they sometimes wear scarves or aprons. Women put on blouse, long skirt, apron, headgear with various decoration and scarf ($pe\v{c}a$), shawl (tibetui robe) or necklace together. Croatian folk dresses fall into three different styles by provinces, such as Pannonia, Dinar and Adria, according to the basic environmental and racial characteristics of each province. People of Pannonia plain generally wear white linen clothing and often use flounce and lace, which are influenced by Serbia and Hungary. In the Dinar mountain province, aprons with tassel, knitted vests made of leather and fur are very popular. The clothing of Dinar province has much commonness with those of Bosnia - Herzegovina and Montenegro which have metal buttons and belts. And the clothing of Adria coast is influenced by Italy a lot. There are many splendid silk clothes in its folk dress code. As mentioned above, there are a lot of foreign influences in Croatian history. Although there were severe cultural invasions in many times through its history, Croatians didn't just surrender to these cultural influences. People in Croatia comply with these new coming culture and reconcile these into their own tradition and dress code in their own way.

• Journal of The Korean Astronomical Society
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• v.46 no.6
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• pp.203-224
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• 2013

Wide-field $JHK_s$ images obtained with the SIRIUS near-infrared camera of the IRSF 1.4m telescope are used to examine the tidal structures of the spatial stellar configuration around six metal-poor ([Fe/H]< -1.0) globular clusters located within 3 kpc from the Galactic center. The radial surface density profiles are obtained from the surface photometry of the cluster images and the star counting for the photometric data. For the star counting, candidates of cluster member stars are selected with an filtering algorithm in color-magnitude diagrams. We find that the six target clusters show tidal overdensity features in the radial surface density profiles. There is a break inside the tidal radius for each cluster, and the profile in the outer overdensity region is characterized by a power law. Two-dimensional density maps of all the clusters show distorted asymmetric stellar configurations in the outer region. In five out of the six target clusters, the overdensity features are likely to be associated with the effects of the Galaxy dynamical interaction and the cluster space motions. The observed tidal configurations of stars suggest that several metal-poor clusters in the Galactic bulge are possibly surviving remnants of mergers to build the old stellar system of the Galactic bulge.

• The Journal of Pediatrics of Korean Medicine
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• v.18 no.2
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• pp.161-177
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• 2004

Objective : There have been many programs to treat childhood obesity, since childhood obesity has shown up as a social problem. The purpose of this study was to find out considerable matters to manage Childhood Obesity Treatment Program (COTP). Methods : This study was made with reference to clinical progress notes of children with obesity, visiting the oriental medicine center, to take COTP from July 30th, 2003 to August 25th, 2004. COTP was consist of abdominal aroma massage, abdominal low frequency acupuncture therapy, auricular acupuncture therapy and behavior modification. Results : 1. Seven children who taken COTP started fat from young age and three started from about ten-year-old ages. 2. Six children have fat fathers, a child has fat parents and three children have no fat family member. 3. The obesity levels of Obesity Index(OI), Body Mass Index(BMI) and Rohler Index(RI) were different, used for evaluating the result of treatment, though they are used for a child. The result of OI reflected more sensitive from changes of weight than those of BMI and RI. 4. We got the better results with numbers of treatment and exercise. Conclusions : More correct standard are needed to estimate degree childhood obesity. And it is necessary to carry out obesity treatment program with behavior modification, for children with obesity. to be healthy adults.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.53 no.4
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• pp.515-523
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• 2020

Basal and heat shock-induced mRNA expression patterns of major heat shock protein (HSP) genes, including those encoding heat shock protein (HSP) 90, HSP70, HSP70-12A, heat shock inducible protein 70 (HSIP70), heat shock binding protein 1 (HSPBP1), HSP60, and HSP40 were examined in the gill and hepatopancreas of 1-year-old diploid and triploid abalone Haliotis discus hannai juveniles. Under non-stimulated conditions at 19℃, triploid abalones displayed, in general, higher mRNA levels of various HSPs (HSP70, HSIP70, HSPBP1, HSP70-12A, and HSP60 in the gill and HSIP70, HSPBP1, and HSP60 in the hepatopancreas) than did communally cultured diploids. Conversely, only the hepatopancreatic expression of HSP70-12A was higher in diploids than in triploids. However, the fold changes in gene expression in response to an acute thermal challenge (elevation from 19 to 30℃) were generally greater in diploids than in triploids, such that the difference in basal expression was diminished, weakened, or even reversed after heat shock treatment. However, unlike other HSP genes, the basal expression of HSP60 (higher in 3N) was more pronounced after heat shock treatment. Collectively, the results of this study suggest that triploid abalones have different capacities for not only basal expression but also the heat-induced expression of HSPs in an HSP member-dependent manner.

• Journal of the Korean Society of Costume
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• v.59 no.7
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• pp.65-76
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• 2009

Korea has established a code for the economic and cultural industry that covers a wide range of classes unlike the old information era which was limited to the simple knowledge, technology, tools, morals, and customers that had been established in our society. On the other hand, 'culture marketing' is a series of marketing activities in which value-added items are created and shared. The combination of business management and culture seemed pretty awkward considering how rapidly the world was changing, however, the nature of today's culture has changed and the economic nature of culture products has resulted from those changes. In this paper, two authors who expressed the cultural identity in the culture products are studied through a comparison analysis; the author Min Yeongsoon expressed the identity confusion that she experienced in a foreign country and how she overcame homesickness in her work; and the author Kim Ata expressed his confidence in Asia and used his clear and loud voice to express the culture in his work. In addition, today's culture products are affected by the surrounding cultures such as the member's traditional society, environmental culture, and individual's culture. It is a good source for good works of art. In this study, the efforts of seeking the cultural identity, structuring the concept, seeking a new market and possibilities are used to make a consistent process in order to make a series of the systems required for planning and developing culture products.