• Journal of Forest and Environmental Science
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• v.34 no.5
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• pp.395-404
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• 2018

Since the mass mortality of Quercus mongolica has been first reported in Gyeonggi province at 2004, the disease spread rapidly over Korean peninsula annually. Ambrosia beetle (Platypus koryoensis) was known as the insect vector of oak wilt fungus, Raffaelea quercus-mongolicae, and control methods of the disease had mainly been focused on eradication of insect vector. However, for the efficient management of the disease, combined control methods for both of the pathogenic fungus and insect vector are strongly required. As one of the efforts to suppress the pathogenic fungus, antifungal activities of Streptomyces isolated from oak forest soil were assayed in this study. Optimum culture condition for the selected isolates was also studied, As a result, Streptomyces blastmyceticus cultured in PDB (Potato Dextrose Broth) at $25^{\circ}C$ for 1 week showed the strongest antifungal activity against oak wilt fungus. Mycelial growth inhibition rates (MGIRs) of Streptomyces isolates were compared on culture media supplemented with heated and unheated culture filtrates of S. blastmyceticus. MGIRs on culture media with unheated culture filtrates were generally higher than those on culture media with heated culture filtrates. Antagonistic mechanism to get involved in the inhibition of hyphal growth and spore formation of the pathogen is due to the antifungal metabolites produced by Streptomyces. This study will provide the fundamental information in developing biocontrol agents for the environment-friendly management of oak wilt disease.

• Journal of Sericultural and Entomological Science
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• v.36 no.2
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• pp.130-137
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• 1994

This experiment was conducted to evaluate the reasonable rearing technique of Japanese oak silkworm under the different rearing condition as environment, feeding materials and rearing methods. The results are summerized as follows. The optimum temperature for the indoor rearing of Japanese oak silkworm was 25~26$^{\circ}C$ a in young silkworm and 22~23$^{\circ}C$ in grown silkworm, and generally in the condition of variable day/night temperature the growth of silkworm was faster than in that of constant temperature. The optimum relative humidity for it's growth was 80~90% at young silkworm stage and 60~70% at grown silkworm stage. The photoperiod was not affected to the growth of Japanese oak silkworm. The earlier brushing, the shorter the young silkworm stage, and the cocoon quality improved compared to the later brushing time. As a feeding materials aok (Auercus acutissima Crruthers) leaves were better than those of chestnut tree (Castanea Crenata Siebold & Zuccarini) and feeding value of cultivated oak tree leaves was better than that of natural oak tree leaves. The moving time when revealed the better cocoon production of Japanese oak silkworm from indoor condition to outdoor was 15 days after hatched, and the cocoon production was decreased by the earlier moving to outdoor.

• Journal of Forest and Environmental Science
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• v.33 no.4
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• pp.342-347
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• 2017

Since the first report of the oak wilt disease at 2004 in Korea, the disease distributed over Korean peninsula and are still giving severe damages. The management of oak wilt disease in Korea has mainly focused on the control of insect vector, Platypus koryoensis. Neverthless the effective method for evaluating the pathogenicity of the pathogen, Raffaelea quercus-mongolicae (Rqm), and for screening chemical or biological agents with strong inhibitory activity against the pathogen, is absolutely necessary, an reliable method is not available so far. This study was conducted to develop the effective method for evaluating the pathogenicity of Rqm in oak trees. The culture suspensions of Rqm were artificially injected to the saplings of Quercus acutissima by using ChemJet tree injector. Three months after treatments, the treated saplings were cut and dipped into 1% fuchsin acid solution. There were significant differences in non-conductive area (%), discoloration area (%) and vertical discoloration length between the pathogen-injected and distilled water-injected control treatments. These results indicated that the pathogen is the causal agent for the dysfunction of water conductive tissue, which will finally result in wilt symptom. Re-isolation of the pathogen and PCR detection using specific primers for the pathogen also confirmed the presence of Rqm in the sapwood chips of the pathogen-injected saplings. These observations would be greatly applied to other related researches for evaluating the pathogenicity of tree wilt pathogens and biocontrol efficacy of the selected antagonistic microorganisms, in case that the wilt symptom is not easily shown by artificial inoculation of the causal agent.

• Landscaping Tree
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• s.113
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• pp.18-19
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• 2009

• Korean journal of applied entomology
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• v.47 no.2
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• pp.127-131
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• 2008

Ambrosia beetle, Platypus koryoensis, is a vector of oak wilt disease caused by Raffaelea sp. in Korea. The degree of damage by oak wilt disease was dependent on the density of the beetles in the oak trunk, a relationship between the degree of damage by oak wilt disease and the density of beetle on the basis of the number of entry hole was studied. Entry hole distribution within tree trunk was analyzed by the nearest neighbor method. Thirty four oak trees (Quercus mongolica) located in survey area were selected and then degree of damage, the number of attack hole/$623cm^2$ in upper (50cm from the surface) and lower (surface) trunk and the nearest neighbor distance between the holes were measured. The number of hole and the nearest neighbor distance in upper and lower part were positively correlated with each other. As the degree of damage was severer, the number of the holes increased, whereas the nearest neighbor distance decreased. The distribution pattern of the hole was changed from clumped one to uniform as the severity of damage increased. These results suggested that Platypus koryoensis attacked the oak tree in concentrative manner at initial stage of attack but at final stage, it distributed uniformly to reduce intraspecific competition between the beetles.

• Journal of Forest and Environmental Science
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• v.34 no.5
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• pp.376-381
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• 2018

For the control of oak wilt caused by Raffaelea quercus-mongolicae, an antifungal microorganism, Streptomyces blastmyceticus, was used as a potential agent. Culture suspension of S. blastmyceticus was injected into Quercus mongolicae in the research forest of Kangwon National University by $ChemJet^{(R)}$ trunk injection and Macro-infusion at root flare injection. $Alamo^{(R)}$ (a.i., propiconazole 14.5%), a fungicide currently used for the control of oak wilt in USA, was also treated by both methods to compare the efficacy. For preventive efficacy, culture suspension of the pathogen was inoculated at 1 month after injection of either agent. Tested trees were cut at 3 months after treatment, stained with 1% Fuchsin acid, and then non-conductive area (NCA) and re-isolation frequency (RIF) of oak wilt fungus were compared among treatments. While NCA was the highest as 47.3% in pathogen only treatment, it was the lowest as 16.0% in sterilized water treatment by Macro-infusion. NCAs of Alamo treatment by Macro-infusion and ChemJet injection were 25.3% and 32.1%, respectively. NCA of S.blastmyceticus treatment by ChemJet injection was 32.3%, similar with Alamo treatment's by ChemJet injection. All treatments by either injection method showed significantly lower NCA compared to the pathogen only treatment. These results indicate that S. blastmyceticus injection shows the preventive efficacy against oak wilt disease by suppressing the growth of pathogen injected. NCA of Macro-infusion injection of sterilized water was lower as 16.0%, compared to 21.3% of ChemJet injection. It means that Macro-infusion is more effective in translocation of sterilized water than ChemJet injection by even distribution. RIF from wood discs of treated trees showed high in pathogen only treatment, but relatively low in S. blastmyceticus treatment. RIF results were correlated with NCA results. From the above results, it was confirmed that S. blastmyceticus showed preventive efficacy against oak wilt disease by ChemJet trunk or Macro-infusion at root flare injection.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.13 no.3
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• pp.36-45
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• 2010

To analyze the relationship between climatic factors (monthly mean temperature and total precipitation) and tree-ring growths of Quercus mongolica Fischer (Mongolian oak) with different topographic sites in Mt. Worak, more than 10 trees were selected from each of seven stands. Two cores from each tree were measured for ring width. After crossdating, each ring-width series was double standardized by fitting first a negative exponential or straight regression line and secondly a 60-year cubic spline. Seven stands were categorized in two groups using cluster analysis for tree-ring index patterns. Cluster I (four stands) was located in higher elevation (550-812 m) with aspects of east, west and northwest, and cluster II (three stands) was located in rather lower election (330-628 m) with aspects of north and northwest. The aspects of two clusters were not significantly different. Response-function analysis showed a significant positive response to March precipitation for both clusters. It indicates that moisture supply during early spring season is important to radial growth because the cambial growths of ring-porous species, such as Mongolian oak, start before leaf growth. Cluster II showed a positive response to the precipitation of middle and late growing season, too.

• Journal of the Korean Wood Science and Technology
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• v.44 no.4
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• pp.515-525
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• 2016

The Palsangjeon Pagoda is a five-story wooden building in the Beopjusa Temple area in the Songnisan national park in Chungcheongbuk-do, Korea. According to historical records, it was constructed in either AD 1605 or AD 1626. To specify the construction year more precisely, we took 41 wood samples for tree-ring analysis during repair actions in 2013; 28 from red pine (Pinus densiflora S. et Z.), 12 from oak (Quercus spp.), and 1 from fir (Abies spp.). The tree-ring boundaries became clearly visible by smoothing their cross-sectional surface and the tree-ring widths were measured under a stereo microscope. All tree-ring series were compared with each other to establish mean chronologies, which we then compared with local master chronologies established using tree rings collected from wooden elements of the Daeungjeon Hall which is the main building in the Beopjusa Temple area. As a result, it was verified that the red pines were felled in AD 1624 and in AD 1851. Moreover, it was verified that the oaks were also felled in AD 1624 based on cross-dating with the red pine chronology. We concluded that the Palsangjeon Wooden Pagoda was constructed in AD 1626, as mentioned in the historical record (Daeungdaekwangmyeongjeonbulsangki, 1630) and the letters on a purlin on the $4^{th}$ story, using oak and red pine felled in AD 1624 and repaired later on using red pine felled in AD 1851.

• Journal of Forest and Environmental Science
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• v.29 no.1
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• pp.29-37
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• 2013

The study was carried out in two different forest types viz., Banj oak and Chir pine forests to assess the variation in forest species composition and soil properties along altitudinal gradients in the Garhwal Himalayas. The results of the study showed that between the forests soil moisture was higher in Banj oak forest because of closed canopy and dense forest compared to Chir pine forest. The sand particles were reported higher in Banj oak forest which might be due to the addition of organic matter favouring coarse structure of soil, helping in holding maximum water in soils. However in the Chir pine forest low amount of soil organic matter and presence of clayey soil, develops soil compactness which reduces the penetration of water resulting in high soil bulk density. The higher accumulation of litter and presence of moisture in Banj oak forest favours higher nutrient level of nitrogen, phosphorus and potassium compared to Chir pine forest. The soil organic carbon also reduced with increasing altitude at both gradients. While bulk density has reverse trend with soil organic carbon in both the forests at different peaks of same region. In Banj oak forest, the highest density and total basal cover was reported 1,100 tree $ha^{-1}$ and 58.86 $m^2\;ha^{-1}$ respectively. However, the highest values of density and total basal cover of Chir pine forest was 560 tree$ha^{-1}$ and 56.94 $m^2\;ha^{-1}$ respectively. The total density and basal cover of both the forests reduced with increasing altitude. The study concludes that Banj oak forest has better nutrient cycling ability, well developed foest floor and has a greater protective and productive features compared to the Chir pine forest which is without lower vegetation cover and having only pine litter accumulation which does not allow any other species to grow.

• Korean Journal of Ecology and Environment
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• v.38 no.4 s.114
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• pp.475-481
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• 2005

In an effort to identify a new environment-friendly algicide, we examined the ability of extracts from the leaves and stems of nine Korean oak tree species to inhibit growth of the bloom-forming cyanobacterium, Microcystis aeruginosa. At a concentration of 100 mg $L^{-1}$, five of the oak tree extracts (QAT-L, QAT-5, QAS- L, QGI-5, and QSA- L) decreased the cell density of M. aeruginosa by over 90% for 7 days. At a concentration of 20 mg $L^{-1}$, the same five extracts inhibited the growth of M. aeruginosa by approximately 50%. The minimum concentration of oak tree extracts required for effective inhibition of M. aeruginosa (20 mg $L^{-1}$) is comparable to that of the known algicide, tannic acid (17 mg $L^{-1}$), which is thought to be one of the main active ingredients in the oak tree extract. These findings suggest that oak extracts may be useful as an environment-friendly algicide to control the bloomforming cyanobacterium, M. aeruginosa, in eutrophic waters.