• Title/Summary/Keyword: nylon thread method

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Transient Middle Cerebral Artery Occlusion Model in Mouse using Nylon Thread (Nylon Thread를 이용한 mouse 에서의 Transient middle cerebral artery occlusion (MCAO) model 확립)

  • Lim, Byung-Chul;Sung, Ji-Hee;Kim, Ha-Na;Park, Seoung-Woo
    • The Journal of the Korea Contents Association
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    • v.19 no.7
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    • pp.186-191
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    • 2019
  • Introduction: In aged people, stroke incidence is increased. But standardized experimental animal protocol study for the research of stroke therapy is rare. There is little report on the success rate of cerebral artery occlusion model using standardized Nylon thread length of precise thread end-size controlled. Method: In this study, the operator intended the occlusion of middle cerebral artery (MCA) using $0.18{\pm}0.02mm$ end 5-0 Nylon thread. Middle cerebral artery occlusion was induced for 60min under isoflurane anesthesia. After 60min, the operator removed the Nylon thread and reperfusion was induced for 23hrs. The mice was killed 23hrs after reperfusion and infarction area of brain was confirmed by 1.5% TTC (2,3,5-tryphenyl tetrazolium chloride) staining. Results: According to end size and insert length of Nylon thread, Middle cerebral artery occlusion (n=50), internal carotid artery occlusion (n= 14), distal middle cerebral artery occlusion (n= 36), anterior cerebral artery (n= 1) were induced. And no infarction (n= 50) was observed. Conclusion: According to weight of mice, the operator induced reversible cerebral artery occlusion model by different insert length (30.0~36.9g : 9.0mm, 37.0~40.0g : 9.5mm) of Nylon thread. Success of cerebral artery occlusion model was confirmed by checking infarction area using TTC staining. The success rate (66.9%, 101/151) of reversible cerebral artery occlusion model in the mouse and the operational conditions are shown.

A field evaluation of two external transmitter attachment methods for small snakes

  • Park Il-Kook;Jeong, Hojun;Park, Daesik
    • Journal of Ecology and Environment
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    • v.46 no.3
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    • pp.183-189
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    • 2022
  • Background: Radio-telemetry is a useful method to investigate the spatial ecology of species in the wild, by tracking the signal of a transmitter attached to an individual. The method of attaching a transmitter to a snake is representatively divided into surgical implantation, and external attachment, which latter is often used in small snake species. Results: In this study, we evaluated and compared the utility of two external attachment methods, the nylon thread method and the tape method, applied to a small grassland snake species of approximately 50 cm snout-vent length, the Slender racer (Orientocoluber spinalis), on an island in the Republic of Korea. The nylon thread method and the tape method were applied to four and five individuals, and radio-tracked for an average of eight and eleven days, respectively. The nylon thread method caused individual injury and disturbed their movement, while using the tape method, the transmitter package sometimes dropped off, but no injury or movement problems occurred. Conclusions: Considering that poor understanding of how to attach the transmitter makes it difficult to investigate the spatial ecology of small snake species, the tape method, evaluated in this study, could be applied to attach a transmitter and to study spatial ecology of such snakes.

Measurement of the interfacial tension value for the polymer blends by breaking thread method (섬유사 붕괴 법(breaking thread)에 의한 고분자 블렌드의 계면장력 측정)

  • Son, Young-Gon
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.8 no.5
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    • pp.1211-1214
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    • 2007
  • In this paper, the experimental details of the interfacial tension measurement by breaking thread method, which measures the interfacial tension between different polymer pairs, are described. The apparatus needed are a hot state, which melts the polymers, and an optical microscopy. The measured interfacial tension values are in goog agreement with the literature's values.

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Redoable Tie-Over Dressing Using Multiple Loop Silk Threads

  • Jo, Hyeon Jong;Kim, Jun Sik;Kim, Nam Gyun;Lee, Kyung Suk;Choi, Jae Hoon
    • Archives of Plastic Surgery
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    • v.40 no.3
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    • pp.259-262
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    • 2013
  • After skin grafting, to prevent hematoma or seroma collection at the graft site, a tie-over dressing has been commonly used. However, although the conventional tie-over dressing by suture is a useful method for securing a graft site, refixation is difficult when repeated tie-over dressing is needed. Therefore, we recommend a redoable tie-over dressing technique with multiple loops threads and connecting silk threads. After the raw surface of each of our cases was covered with a skin graft, multiple loop silk thread attached with nylon at the skin graft margin. We applied the ointment gauze and wet cotton/fluffy gauze over the skin graft, then fixed the dressing by connecting cross-counter multiple loop thread with connecting silk threads. When we opened the tie-over dressing by cutting the connecting silk threads, we repeated the tie-over dressing with the same method. The skin graft was taken successfully without hematoma or seroma collection or any other complications. In conclusion, we report a novel tie-over dressing enabling simple fixation of the dressing to maintain proper tension for wounds that require repetitive fixation. Further, with this reliable method, the skin grafts were well taken.

A Study on the E-textiles Dip-Coated with Electrically Conductive Hybrid Nano-Structures

  • Lee, Euna;Kim, Jongjun
    • Journal of Fashion Business
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    • v.21 no.6
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    • pp.16-30
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    • 2017
  • Currently, e-textile market is rapidly expanding and the emerging area of e-textiles requires electrically conductive threads for diverse applications, including wearable innovative e-textiles that can transmit/receive and display data with a variety of functions. This study introduces hybrid nano-structures which may help increase the conductivity of the textile threads for use in wearable and flexible smart apparels. For this aim, Ag was selected as a conductive material, and yarn treatment was implemented where silver nanowire (AgNW) and graphene flake (GF) hybrid structures overcome the limitations of the AgNW alone. The yarn treatment includes several treatment conditions, e.g., annealing temperature, annealing time, binder material such as polyurethane (PU), coating time, in order to search for the optimum method to form stable conductive nano-scale composite materials as thin film on the surface of textile yarns. Treatedyarns showed improved electrical resistance readings. The functionality of the spandex yarn as a stretchable conductive thread was also demonstrated. When the yarn specimens were treated with colloid of AgNW/GF, relatively good electrical conductivity value was obtained. During the extension and recovery cycles of the treated yarns, the initial resistance values did not deteriorate significantly, since the network of nanowire structure with the support of GF and polyurethane stayed flexible and stable. Through this research, it was found that when one-dimensional structure of AgNW and two-dimensional structure of GF were mixed as colloids and treated on the surface of textile yarns, flexible and stretchable electrical conductor could be formed.