• Asian-Australasian Journal of Animal Sciences
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• v.6 no.2
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• pp.197-203
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• 1993

The protection of dry matter and nitrogen from rumen degradation of dhaincha seed (Sesbania aculeate) meal was studied using nylon bag technique. The dhaincha seed meal was subjected to various heat treatments that included oven drying, autoclaving and boiling. Similar experiment was conducted with fish meal as reference for comparison. The oven-dried dhaincha meal was found to retain more dry matter and nitrogen than was found boiled or autoclaved meal. While autoclaving appeared to improve nitrogen and dry matter retention to some extent, boiling seemed to cause more loss of dry matter from nylon bag. Heat treatment caused high retention of nitrogen by fish meal. The calculated effective protein degradation was 80.4% and 83.2% for the oven dried fish meal and dhaincha seed meal whereas same values were 74.2% and 86.7% for autoclaved fish and dhaincha seed meal respectively at the outflow rate of 4.4% per hour. The in vitro study revealed higher digestibility for heat treated samples by pepsin. The dry matter, nitrogen and ash content of dhaincha seed meal were 85.93%, 5.93% and 7.31% respectively.

• Asian-Australasian Journal of Animal Sciences
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• v.10 no.2
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• pp.210-214
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• 1997

Ruminal degradation characteristics and intestinal availability of crude protein (CP) in four animal-origin feeds (fish meal, meat meal, viscera meal, feather meal) were estimated by mobile nylon bag technique. Three ruminally and duodenally cannulated Holstein dairy cows (average body wt. 550kg) fed a diet containing 40% concentrate and 60% orchard grass hay on a dry matter (DM) basis. Assuming that the outflow rate of diet in rumen is 5% per hour (k =0.05), contents of quickly degradable CP (QDP), slowly degradable CP (SDP), and undegradable CP (UDP) in the rumen were 27.6%, 9.4%, 63.0% for fish meal, 34.3% 28.1%, 37,6% for meat meal, 43.9%, 12.5%, 43.6% for viscera meal, and 14.4%, 15.8%, 69.8% for feather meal, respectively. Intestinal CP degradability was 51.0% for fish meal, 27.2% for meat meal, 37.9% for viscera meal and 56.2% for feather meal. Available UDP in the intestinal tract was contained 288 g, 217 g, 246 g and 423 g per kilogram DM of diet in fish meal, meat meal, viscera meal and feather meal, respectively.

• Asian-Australasian Journal of Animal Sciences
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• v.8 no.5
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• pp.433-441
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• 1995

A series of experiments was conducted to determine the influence of various pepsin-HCL pretreatment factor, hereby the factors of duration of washing for the retrieved bags, inherent to the mobile nylon bag technique (MNBT), on apparent ileal digestibility of crude protein (AIDCP) and apparent ileal digestibility of dry matter (AIDDM). At last, the AIDCP and apparent ileal digestibility of amino acids (AIDAA) in maize, barley, wheat, rapeseed meal, cottonseed meal and three mixed diets were determined with the MNBT and ileo-rectal anastomis pigs (IRAT). For the MNBT techniques, bag measuring $25{\times}40$ MM and containing 0.75 g feedstuff samples, after pre-digestion in vitro, were introduced into the ileo-rectal anastomis pigs (IRAT) gastrointestinal tract through a duodenal cannula and recovered in the ileal digesta between 6 and 12 h. later. 1. The apparent ileal digestibility of dry matter (AIDDM) and crude protein (AIDCP) of the tested samples, with the exception of fish meal, determined by MNBT were not affected by the different pepsin-HCL pretreatment times in vitro between 2.5 h. and 4 h. 2. There was no significant (p > 0.05) difference of the AIDCP and AIDDM of maize determined by the MNBT among different pepsin concentration (0.03%, 0.07% and 0.1 %) treatment in vitro. 3. The AIDCP determined with the MNBT was affected by the washed and unwashed recovered bags from the ileal digesta. 4. The AIDCP and AID amino acids (AIDAA) of maize, barley, wheat, rapeseed meal, soya-bean meal, cottonseed meal and three mixed diets from the MNBT, with a solution of 0.01N HCL (PH 2) and 0.1% of pepsin concentration, a pepsin-HCL pretreatment time in vitro or 4h. and a washing time of the recovered bag from the ileal digesta compared well with those from the IRAT. The linear regression analysis showed a significant correlation (p < 0.01) of AIDCP and AIDDA between the IRAT and MNBT.

• Journal of The Korean Society of Grassland and Forage Science
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• v.13 no.1
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• pp.23-30
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• 1993

This study was investigated about decomposition process of litter that is reduction pathway of phosphorus from plant body to soil That is, in each community of Miscanthus sinensis, SaSa palmalta. Artemisia princeps and Polygonum thunbergii, disappearing speed was calculated from total fallen leaves yield supplied as litter and litter existant yield. Besides, setting up litter bag that put litter in nylon mesh bag. calculated disappearing speed from decreasing speed of the weight of contents and then was compared and examined. The results obtained are summarized as follows : 1. Maximum litter yield was sequently Miscanthus sinensis＞SaSa palmalta＞Artemisia princeps＞Polygonum thunbergii, but, supplied litter yield was sequently Artemisia princeps＞Miscanthus sinenis＞Polygonum thunbergii＞SaSa palmalta. 2. Reduction speed of phosphorus from plant body to soil was Polygonum thunbergii＞Artemisia princeps＞Miscanthus sinensis＞SaSa palmalta. 3. Caculated disappearing speed using litter bag method was shown latter tendency than that of natural condition. 4. It was significantly negative relationship between N contents of litter and disappearing speed of litter.

• Asian-Australasian Journal of Animal Sciences
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• v.9 no.6
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• pp.637-642
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• 1996

This experiment was conducted to determine RDP values of locally available feedstuffs that are commonly used in ruminant rations in Bangladesh. Four cattle were fistulated in the rumen for the in situ nylon bag studies. Seventeen different feedstuff sample (9 roughages and 8 concentrates) were evaluated in $4{\times}14cm$ nylon bags and incubated in the rumen for different periods of time (2, 6, 12, 24, 48 and 72 h). The variation in crude protein (CP) contents reflected on the average CP disappearance value throughout the rumen incubation. Soluble fraction (a), insoluble but degradable fraction (b) along with the rate of degradation also varied widely among the various feedstuffs. Under 2% of rumen outflow rate, the percentages of the calculated protein degradabilities of roughages were rice straw, 16.7; maize grass, 70.6; oat grass, 70.8; dhal grass, 71.1; sunhemp, 78.4; napier grass, 62.4; matikalai grass, 72.1; khesarikalai grass, 76.9 and daincha browse, 78.4, respectively. The results in the protein degradabilities (%) in 8% ruminal outflow rate of concentrates were wheat bran, 61.6; rice polish (red), 61.3; rice polish (auto), 30.9; mustard oil cake, 71.8; sesame oil cake, 74.2; coconut oil cake, 57.9; soybean meal, 49.2 and fish meal, 37.9, respectively.

• Asian-Australasian Journal of Animal Sciences
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• v.2 no.3
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• pp.413-415
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• 1989

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.7
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• pp.73-83
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• 2017

This study was conducted to evaluate the chemical composition, digestibility, and energy value of 15 non-conventional feeds produced in South Korea as ruminant feeds. Three Hanwoo steers (body weight, $520{\pm}20.20kg$) fitted with a permanent rumen cannula and duodenal cannula were housed individually in tie-stall barns, followed by a 14-day adaptation period and 3-day experimental period. Chemical composition analysis, in situ nylon bag, and mobile bag technique were used as experiments. As a result of the chemical composition analysis offeeds, crude protein (CP) contentsofmalt meal, perilla meal, soy sauce cake, and soymilk residue were greater than 30%. As a result of the degradability characteristics analysis of feeds using an in situ nylon bag, rumen undegraded protein (RUP) contents of beet pulp, brewer's grain, coffee meal, malt meal, milo bran, perilla meal, ramen residue, and soymilk residue were greater than 50%. Analysis of total digestible nutrient (TDN) values of feeds using an in situ mobile bag showed that TDN values of beet pulp, brewer's grain, makgeolli residue, milo bran, perilla meal, ramen residue, rice bran, soy sauce cake, soybean curd cake, soymilk residue, and wheat bran weregreater than 50%. In summary, these non-conventional feeds have high potential value as good feed resources to replace formulated feeds or roughage. Therefore, the chemical composition, digestibility, and energy value of non-conventional feeds obtained from this study can be used as base data for the manufacture of ruminant total mixed ration (TMR) with improved feed efficiency, reduced feed costs, and reduction of environmental pollution.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.6
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• pp.914-919
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• 2007

The effect of the addition of exogenous fibrolytic enzymes (mainly xylanase and cellulase activities, 15 ml/15 kg of fresh forage), on intake, total tract digestibility and nylon bag degradability of a chopped fresh Digitaria decumbens grass was studied at 2 stages of regrowth ( 21 and 56-day old grasses). Moreover, comparisons between ground and chopped grass were done using the nylon bag degradability method. DM intake (g/kg $BW^{0.75}$) and organic matter total tract digestibility for control and enzyme treatments respectively were 69.1 vs. 65.9 (p>0.05) and 0.723 vs. 0.727 (p>0.05) with the 21-day old regrowth. Based on the same parameters, values for the 56-day old grass were 58.1 vs. 52.7 (p>0.05) and 0.621 vs. 0.591 (p>0.05). Nylon bag degradation at 24 h of the dry matter for control versus enzyme treatments were 0.653 vs. 0.70 (p<0.05) and 0.644 vs. 0.733 (p<0.0001) for the 21-day old chopped and ground forage respectively, whereas with the 56-day old grass, corresponding values were 0.321 vs. 0.392 (p<0.0001) and 0.463 vs. 0.481 (p>0.05). The positive impact of exogenous fibrolytic enzymes (EFE) on degradability of the young and ground pangola grass may suggest that in some cases, enzyme accessibility to potentially digestible cell wall is a limiting factor in their digestion.

• Proceedings of the KAIS Fall Conference
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• 2009.05a
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• pp.368-371
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• 2009

선박용 침몰방지용 부양백의 소재를 개발하는데 있어 부양백 내의 부양기체인 질소가스가 장기 유지특성 보유 소재(gas barrier properties), 코팅 경량 직물형태로서 경량성 보유 소재 기술, 장수명의 부양 백 소재 개발, Silicone/Nylon/Silicone 코팅 다층 구조의 고강도 유지 특성 유지 및 재활용성 특성 유지 부양백 소재를 개발하였다. 이들 소재를 개발하는데 있어 Nylon 직물별 물성 조사로 우리가 개발 목표로 하는 nylon의 denier 별로 구매하여 이들에 대한 기본물성인 인장특성, 내노화성, 기체투과도 및 복원성을 조사하였으며, Nylon 직물에 Silicone의 코팅시험을 실시하였고, 최적 코팅 silicone의 선정을 통해 최적의 균일한 두께 특성, 최적의 강도 유지특성 및 gas barrier 특성을 갖는 silicone 및 코팅두께를 선정하여 최종적으로 부양백용 gas barrier성 수지의 선정하였다.

• Asian-Australasian Journal of Animal Sciences
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• v.29 no.9
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• pp.1273-1279
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• 2016

The objectives of this study were to determine an optimal cultivation time for populations of yeast and lactic acid bacteria (LAB) co-cultured in fermented milk and effects of soybean meal fermented milk (SBMFM) supplementation on rumen degradability in beef cattle using nylon bag technique. The study on an optimal cultivation time for yeast and LAB growth in fermented milk was determined at 0, 4, 8, 24, 48, 72, and 96 h post-cultivation. After fermenting for 4 days, an optimal cultivation time of yeast and LAB in fermented milk was selected and used for making the SBMFM product to study nylon bag technique. Two ruminal fistulated beef cattle ($410{\pm}10kg$) were used to study on the effect of SBMFM supplementation (0%, 3%, and 5% of total concentrate substrate) on rumen degradability using in situ method at incubation times of 0, 2, 4, 6, 12, 24, 48, and 72 h according to a Completely randomized design. The results revealed that the highest yeast and LAB population culture in fermented milk was found at 72 h-post cultivation. From in situ study, the soluble fractions at time zero (a), potential degradability (a+b) and effective degradability of dry matter (EDDM) linearly (p<0.01) increased with the increasing supplemental levels and the highest was in the 5% SBMFM supplemented group. However, there was no effect of SBMFM supplement on insoluble degradability fractions (b) and rate of degradation (c). In conclusion, the optimal fermented time for fermented milk with yeast and LAB was at 72 h-post cultivation and supplementation of SBMFM at 5% of total concentrate substrate could improve rumen degradability of beef cattle. However, further research on effect of SBMFM on rumen ecology and production performance in meat and milk should be conducted using in vivo both digestion and feeding trials.