• 제목/요약/키워드: nutritional source

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Amylase와 Protease의 활성이 높은 현미 발효 미생물의 선별 (Screening for Fermentative Microorganisms that Grow on Brown Rice with High Amylase and Pretense Activities)

  • 김기연;김희규;송병철;차창준
    • 미생물학회지
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    • 제42권2호
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    • pp.160-163
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    • 2006
  • 현미는 백미에 비하여 식품영양학적 가치 및 식이섬유의 함유량이 높은 쌀겨 및 배아를 포함하고 있으나 소화가 잘되지 않는 단점 이 있다. 따라서 발효현미는 소화력을 높임과 동시에 좋은 영양 공급원이 될 수 있으므로 높은 amylase와 pretense의 활성으로 현미를 발효할 수 있는 미생물을 선별하였다. 2.5%(w/v)의 현미분말을 유일한 영양원으로 한 액체배지를 생장배지로 사용하여 생장능력과 효소 생산능력을 조사하였다. 조사한 8종의 Bacillus 와 11종의 유산균 중에서 모든 Bacillus 균주와 두 종의 유산균이 생장과 효소활성을 보였다. 생균수는 $10^7CFU/mL$을 초과하였으며 Bacillus sp. Bacillus sp. B2, Bacillus sp. B11, Leuconostoc gelidum, Pediococcus pentosaceus 가 생산하는 최고 amylase 활성은 각각 17.85, 17.50, 17.10, 17.10, 3.24 U/ml이었고, 최고 pretense 활성은 각각 22.48, 22.04, 23.76, 12.13, 3.80 U/ml이었다. 따라서 이 균주들은 발효 현미 제조를 위한 접종균주로서 이용이 가능하리라 사료된다.

국내식품을 이용한 이유식 개발에 관한 연구(I) - 이유식의 개발과 성분 및 영양소 분석 - (Development of the Supplementary Foods for Infants Using Korean Foods - Development and Analysis of Nutrients of the Supplementary Foods -)

  • 민성희;손경희;윤선
    • 한국식품조리과학회지
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    • 제9권2호
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    • pp.99-104
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    • 1993
  • This study was carried out in order to develop supplementary foods for infants using Korean foods. Thirty-four different kinds of supplementary foods were developed and fourteen representative ones were selected to be analyzed chemically. The results are as follows: 1. The developed supplementary foods were 34 kinds and divided into 3 stages. First stage is designed for the babies just beginning to eat pureed vegetables and fruits. Second stage combined the nutritional attributes of both vegetables and meat. Third stage featured tender, bite-size pieces of meats and vegetables that appealed to the most mature tastes of basies. 2. In the production of prepared foods; water, milk, vegetable juice, fruit juice, and soy milk were used as the liquid source; rice, rice starch, chestnut, noodle, potatoes, sweet potatoes, rice cakes as the carbohydrate source; fish, meats soybean curd, beans, eggs, chicken, cow liver as the protein source; and vegetables and fruits were used as vitamin & mineral source. 3. The approximate composition range of the products were 10.91∼24.46% carbohydrate, 0.15∼6.06% protein, 0.092∼7% fat, 0.13∼ 1.37% ash, 0.63∼36.34% calcium, 0.092∼0.48% iron and 0.42∼16.36% vitamin C.

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Physiological and Nutritional Factors for Efficient Sporulation and Toxin Formation in Bacillus tthuringiensis

  • 이은희;반재구;김정일
    • 한국미생물생명공학회:학술대회논문집
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    • 한국미생물생명공학회 1986년도 추계학술대회
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    • pp.522.2-522
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    • 1986
  • In order to optimally induce sporulation and toxin formation in Bacillus thuringiensis, exhaustion of specific nutrients as well as resuspension experiments were tried. Sporulation and toxin formation was most abunduntly occurred when the growth was limited by carbon source. It was also occurred in a resuspension medium containing only distilled water. Various environmental and physiological factors affecting the efficiencies of spore and toxin formation were examined in chemically defined media. As a result of these studies, a batch fermentation resulted in higher spore and toxin yield than ever reported

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A Review on the Role of Duckweed in Nutrient Reclamation and as a Source of Animal Feed

  • Goopy, J.P.;Murray, P.J.
    • Asian-Australasian Journal of Animal Sciences
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    • 제16권2호
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    • pp.297-305
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    • 2003
  • The family of lemnacae colloquially known as duckweed contains the world' smallest species of flowering plants (macrophytes). Aquatic and free-floating, their most striking qualities are a capacity for explosive reproduction and an almost complete lack of fibrous material. They are widely used for reducing chemical loading in facultative sewage lagoons, but their greatest potential lies in their ability to produce large quantities of protein rich biomass, suitable for feeding to a wide range of animals, including fish, poultry and cattle. Despite these qualities there are numerous impediments to these plants being incorporated into western farming systems. Large genetically determined variations in growth in response to nutrients and climate, apparent anti-nutritional factors, concerns about sequestration of heavy metals and possible transference of pathogens raise questions about the safety and usefulness of these plants. A clear understanding of how to address and overcome these impediments needs to be developed before duckweed is widely accepted for nutrient reclamation and as a source of animal feed.

플라스크 배양에서 Bacillus subtilis BK-17의 혈전용해효소 생산에 대한 환경 및 영양 조건의 영향 (Effects of Environmental and Nutritional Conditions on Fibrinolytic enzyme Production from Bacillus subtilis BK-17 in Flask Culture)

  • 최원아;이진욱;이경희;박성훈
    • KSBB Journal
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    • 제13권5호
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    • pp.491-496
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    • 1998
  • The production of fibrinolytic enzyme from Bacillus subtilis BK-17 was studied in the shake flask cultures. The important medium components studied include nitrogen source, carbon source and inorganic salts. The environmental conditions include initial pH, temperature, shaking speed and working volume. Among various N-sources, C-sources and inorganic salts tested, soybean flour, D-glucose and Na2HPO4 gave the best results, and their optimal concentrations were 1.5%, 0.5% and 0.05%, respectively. The optimal pH and temperature were 9.0 and 37$^{\circ}C$. With decreasing working volume in the range of 25∼100ml in the 250ml flask or increasing shaking speed in the range of 100∼300rpm, the enzyme production was greatly enhanced. The enzyme activity under the optimal conditions was about 1400I.U./ml with urokinase as a standard.

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Xylose 발효효모의 Xylanase 생성 (Nutritional Conditions of Xylanase Production from Xylose Fermenting Yeast)

  • 배명애;김남순;방병호;서정훈
    • 한국미생물·생명공학회지
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    • 제17권2호
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    • pp.85-87
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    • 1989
  • 토양으로부터 분리한 Xylose 발효효모 X-6-41 (Candida sp.)의 xylanase 효소생성 조건을 조사해 본 결과, 본 실험에 사용한 X-6-41 균주는 배지조성중 yeast extract 혹은 polypeptone이 첨가되면 xylanase 효소생성이 없었다. 즉 특정 아미노산인 isoleucine에 의해서는 그 생성이 억제되었고, tryptophan 25$\mu$g/$m\ell$ 농도의 존재하에서는 xylanase 생성이 증가했다.

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식품단백질 효소분해물의 제조 및 이화학적 특성 (Preparation and Chemical Characteristics of Food Protein Hydrolysates)

  • 김종희;홍순광
    • 동아시아식생활학회지
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    • 제19권1호
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    • pp.45-51
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    • 2009
  • In this study, food protein hydrolysates were prepared from six types of food protein: purified meat protein, whole egg protein, casein, isolated soy protein, concentrated rice protein, and gluten. Food proteins were hydrolyzed with pepsin and ethanol (80%)-soluble fractions of pepsin hydrolysates were employed for analysis. The products were colorless and odorless powders with low fat content and good solubility. The MW (molecular weight) of the protein hydrolysates was confirmed to be $200{\sim}1,800$ via gel filtration. Free amino acid contents accounted for less than 5% of the samples. The results of our amino acid analysis revealed that all food protein hydrolysates preserved their original amino acid compositions and nutritional values of their source proteins with highly pure oligopeptide mixtures. These results show that the food protein hydrolysates prepared in these investigations should prove excellent dietary nitrogen sources for a variety of applications.

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푸드뱅크(Foodbank) 사업 수혜자의 실태 분석 및 이용편익 평가 (Investigation of the Conditions and Evaluation of the Benefits of the Foodbank Program from the Recipients Perspective)

  • 강혜승;양일선;이영선
    • 대한지역사회영양학회지
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    • 제8권2호
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    • pp.231-239
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    • 2003
  • The purposes of this study were to: (a) investigate the characteristics of recipients' of the non-government foodbank program, (b) examine the health and dietary related conditions of them, and (c) evaluate the benefits and effectiveness of the foodbank program from the recipients' perspective. A total of 21 groups (n = 755) and 75 individual recipients participated in the survey. The main results of the study were as follows; (a) Generally, the individual recipients were 74-year-old female, livelihood protectee, and those who received government assistance or funds from private donators as their source of livelihood. (b) The ages of group recipients varied widely, and they also received government assistance or funds from private donators as their source of livelihood. (c) Most of the donated foods were bakery and confectionery Items. rice, and milk and other dairy products. (d) Benefits such as the decrease in the recipients' food expenses and an enhancement of their nutritional statuses were identified. (Korean J Community Nutrition 8(2) : 231∼239, 2003)

Streptomyces 속 균주가 생성한 물질의 생물활성 (제삼보) 정제 및 영양요구성 (Biological Active Substance Produced by a Strain of Streptomyces sp. (Part.III) Purification and Nutritional Requirement.)

  • 송방호;서정훈
    • 한국미생물·생명공학회지
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    • 제5권1호
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    • pp.36-45
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    • 1977
  • 공시균의 배양여액으로부터 독성물상을 chloroform 에 전용하여 농축한 후 avicel column chromatography 및 avicel TLC로 정제하였다. 독성물질 생성에 요구되는 영양원은 생육에 필요한 영양첨과 일치하지 않았으며 Valine, asparagine, arginine, 요소, D-glucose, D-mannose, L-rhamnose, D-ribose, D-xylose, D-arabitol, starch 등이 독성물질생성에 요구됨이 인정되었다. 또한 오탄당의 대사과정중 ribose, xylose, fructose, glucose가 대사되는 과정이 본 물질생성의 주된 경로로 추정된다. Vitamin류는 무관함에 비해 Mg가 필수적으로 요구되었다. 물질의 생성은 균의 증식에 비례되었으며 6일간 3$0^{\circ}C$에서 배양했을때 가장 많이 생성되었다. 독성물질 처리후의 잉어 조직은 아가미 신장 췌장등에서 핵진의 굴곡과 더불어 접의 심한 수축과 세포질의 괴사가 현저하였다. 본 물질의 화학식은 $C_{38}$ $H_{66}$ $NO_4$로 추정되며 UV조사시 회청색형광을 나타내었다.

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