• Journal of Nutrition and Health
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• 제4권1호
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• pp.1-19
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• 1971

Nutritional anemia is an important nutritional problem affecting large population groups in most developing countries. Nutritional anemia is caused by the absence of any dietary essential involed in hemoglobin formation or by poor absorption of these dietary components. The most likely causes are lack of dietary iron, and folate, vitamin $B_{12}$ and high qualify protein. Anemia is considered to be a late mainfeastation of nutritional deficiencies, and even mild anemia is not the earilest sign of such a deficiency. Therefore, the object of therapy is to correct underlying deficiency rather than merely its manifestation. Iron deficiency anemia is generally much the most common form of anemia. And it is very prevalent particularly in pregnant women and young children, especially under five year of life. According to the rapid growth rate of infants, dietary iron should he provided for infants over three months of age in adequate amounts for the synthesis of hemoglobin required by the increasing blood volume and for the demands of newly formed cells. The principal causes of iron deficiency anemia are an inadequate dietary iron content, interference with absorption of iron from the intestine, excessive losses of iron from the body, disturbance of iron metabolism by infection, and social and cultural environments. The present study is planned to obtain informations concerning nutritional anemia through anthropometric and biochemical determinations for the assessment of nutriture in pre-school children. Determination was taken in 226 pre-school children in ruraI arae in 1968, 122 pre-school children in 1970, and 1526 hospitalized pre-school children in 1970. The results of this study are as follows; (1) According to Iowa Malnutrition Borderline (85 percentile) for weight, the proportions of underweighed pre-school boys and girls in rural area were 47.2% and 46.2% in1968, and were 36.1% and 51.8% in 1970. According to Iowa Malnutrition Borderline for height, the proportions of underheight boys and girls in rural area were 30.5% and 33.7%, and were 26.2% and 21.8% in 1970. Malnutrition scores of underweight for height values of boys and girls in rural area were 19.3 and 17.3 in 1968, and the scores of boys and girls were 15.6 and 15.5 in 1970. (2) The mean hemoglobin values of boys and girls in rural area were $11.2{\pm}1.8g/100ml\;and\;11.4{\pm}1.6g/100ml$ in 1968. In 1970, the mean values of boys and girls in rural area were $11.3{\pm}1.3g/100ml\;and\;11.7{\pm}2.4g/100ml$. The mean hemoglobin values of hospitalized boys and girls were $11.9{\pm}2.2g/100ml\;and\;11.7{\pm}2.4g/100ml$ in 1970. It is found that 92 of 215 children (42.7%) in rural area had concentrations of hemoglobin less than 11.0g/100ml in 1968. In 1970, 55 of 121 children (45.4%) in rural area and 559 of 1526 hospitalized children (36.6%) had concentrations of hemoglobin less than 11.0g/100ml. (3) The mean hematocrit levels of hospitalized boys and girls were $35{\pm}26.8%\;and\;35.4{\pm}6.4%$ in 1970. And 443 of 1334 hospitalized children (33.2%) had hematocrit values below 33%. (4) The average mean corpuscular hemoglobin concentration levels of hospitalized boys and girls were $32.4{\pm}2.2\;and\;32.3{\pm}2.2$ in 1970. And 1016 of 1352 hospitalized children (75.1%) had the mean corpuscular hemoglobin values below 34. (5) The mean iron values of young children in rural area and hospitalized children were $62.0{\pm}6.3{\mu}g/100ml\;and\;60.7{\pm}22.8{\mu}g/100ml$. The proportions of anemia cases below $50{\mu}g/100ml$ in rural area was 37.9%, and 34.3% in hospitalized children. (6) The mean total iron binding capacity of young children in rural area was $376{\pm}57.88{\mu}g/100ml,\;and\;342.2{\pm}6.15{\mu}g/100ml$ in hospitalized children. (7) The average transferrin saturation percentage of young children in rural area was $16.9{\pm}4.7%,\;and\;18.0{\pm}8.4%$ in hospitalized children. The proportions of anemia cases below 15% of young chi1dren in rural area and hospitalized children were 48.3% and 41.2%. Therefore, authors wish to recommend that the following further studies should be undertaken: (1) Standardization of simplied laboratory examination of nutritional anemia. (2) The prevalence of nutritional anemia and the requirements of iron, folate, and vitamin $B_{12}$ of pre-school children. (3) The content and absorption of iron in Korean food. (4) The pathogenesis of nutritional anemia and prevention of parasitic disease. (5) Maternal health and nutrition education.

• 한국식품영양과학회지
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• 제12권3호
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• pp.273-283
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• 1983

흰쥐와 흰생쥐 암 수 각 30마리씩에 대한 실험개시기와 종료후의 결과로서 나타난 식이에 대한 사양실험, 대사실험, 혈액성분분석 실시 결과는 다음과 같다. 1. 증체량은 흰쥐 암 수 모두 동물성단백질 배합비율이 높아질수록 증가했다. 흰생쥐는 숫생쥐가 C식이에서 최대를, E식이에서 최저를 나타냈으나 암생쥐는 반대였다. 2. 식이효율은 흰쥐 암 수 모두 동물성 단백질 배합비율이 높아질수록 증가했으며 암쥐보다 숫쥐에서 높았다. 흰생쥐는 숫생쥐가 증체량과 같은 경향이었으나 암생쥐는 일정경향이 없었다. 암생쥐보다 숫생쥐의 식이효율이 높았다. 3. 단백질효율은 흰쥐 암 수 모두 동물성단백질 배합비율이 높아질수록 증가했다. 흰생쥐는 숫생쥐의 증체량, 식이효율은 같은 경향이나 암생쥐는 일정한 경향이 없었다. 4. 질소보유율은 흰쥐 암 수 모두 단백질수준이 같을때 섭취질소량에 비례함을 보였다. 흰생쥐는 숫생쥐가 동물성 배합비율이 높아질때 증가하였다. 암생쥐는 일정한 경향이 없었다. 질소축적율은 흰생쥐에서 높게 나타나 체내 단백질이용이 흰쥐보다 높음을 알 수 있었다. 5. 혈청성분에서는 혈청 총단백질량은 흰쥐 암 수 모두 차이가 없으나 동물성단백질 배합비율이높아질수록 증가하는 경향이었다. 흰생쥐는 암 수 모두 일정한 경향이 없었다. 혈청요소질소량은 흰쥐의 경우 암 수 모두 식물성단백질 배합비율이 높아질수록 증가했다. 흰생쥐는 알정한 경향이 없었다. 혈청콜레스테롤량은 흰쥐 암 수 모두 단백질배합비율에 관계없이 일정한 경향이 없었다. 흰생쥐는 숫생쥐가 식물성단백질 배합비율이 높아질수록 증가했으나 암생쥐는 일정한 경향이 없었다. 6. 위의 결과로 실험식이중의 동물성단백질 배합비율이 높은 A, B식이에서 흰쥐는 증체량, 사료효율, 단백질효율이 좋았다. 흰생쥐는 숫생쥐가 C식이, 암생쥐가 E식이에서 높게 나타났다. 이는 생쥐의 증체량이 이유에서부터 성숙기까지 큰 차이가 없다는 것과 흰쥐와는 다른 단백질수준이 요구된다고 생각된다.

• Journal of Nutrition and Health
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• 제51권6호
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• pp.538-555
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• 2018

본 연구에서는 2013 ~ 2016년 국민건강영양조사 자료를 이용하여 19 ~ 64세 성인 남녀의 커피 섭취 종류와 섭취량(정량적 식품섭취빈도조사 자료 활용)에 따른 일반사항, 총 당류를 포함한 영양섭취상태, 건강관련 삶의 질 및 대사증후군과의 관련성을 분석하였다. 남성과 여성 모두에서, 에너지 섭취량이 에너지필요추정량 (EER) 미만인 사람과 철분 섭취량이 평균필요량 (EAR) 미만인 사람의 비율이 3-in-1 커피 섭취군에서 낮았다. 커피를 섭취하지 않는 여성과 비교했을 때, 3-in-1 커피를 하루 2회 이하로 섭취하는 여성은 EQ-5D를 통해 조사한 건강 관련 삶의 질저하에 대한 위험이 19% 낮은 것으로 나타났으며, 남성과 여성 모두에서 섭취하고 있는 커피의 종류와 섭취량에 따라 대사증후군 관련 지표 및 대사증후군에 대한 위험에 차이가 없었다. 따라서 3-in-1 커피 섭취가 건강관련 삶의 질저하에 대한 낮은 위험과 관련이 있었으나, 대사증후군 및 관련 지표에 대한 위험과는 관련이 없다는 결론을 얻을 수 있었다. 앞으로 3-in-1 커피 섭취가 건강관련 삶의 질 및 대사증후군 나아가 사망률까지 이어지는 건강에 미치는 영향에 대한 기전을 파악하기 위해 이와 관련하여 잘 설계된 무작위 임상비교시험이나 코호트 연구가 필요하다.

• Journal of Nutrition and Health
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• 제1권2호
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• pp.107-115
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• 1968

Number of aspects, not only nutritional but social as well as political involved in human starvation pose nowadays global problems. In order to help establish the minimum nutritional requirements in the daily life of a man and to free people as well from either undernourishment, malnutrition or even starvation many workers have devoted themselves so far on the research programs to know what and how number of metabolic events take place in animals in vivo. It is the purpose of the present paper to examine in effect to what extent both of the protein and nucleic acids (DNA & RNA) together with an enzyme, guanine deaminase, which converts guanine into xanthine and in turn ends up to uric acid as an end product, undergo changes, quantitatively during acute starvation, using the mouse as an experimental animal. The mouse was strictly inhibited from taking foods except drinking water ad libitum and was sacriflced 24, 48, and 72 hours following starvation thus acutely induced. The animals consisted of two experimental groups, one control and another starvation groups, each being consisted of 6-24 mice of whose body weights ranged in the vicinity of 10 g. The animals were sacriflced by a blow on the head, followed by immediate excision of their livers into ice-cold distilled water, washing adherent blood and other contaminant tissues. The liver was minced foramin, by an all-glass homogenizer immersing it in an ice-bath, followed by subsequent fractionatin of the homogenate (10% W/V in 0.25M sucrose solution made up with 0.05M phosphate buffer of pH 7.4). For the liver protein and guanine deaminase assay, the 10% homogenate was centrifuged at 600 x g for 10 minutes to eliminate the nuclear fraction; and for the estimation of DNA and RNA, the homogenate was prepared by the addition of 10% trichloroacetic acid in order to free the homogenate from the acid-soluble fraction, the remaining residue being delipidate by the addition of alcohol and dried in vacuo for later KOH (IN) hydrolysis. The changes in body and liver wegihts during acute starvation were checked gravimetrically. Protein contents in the liver were monitored by the method of Lowry et al; and guanine deaminase activities were followed by the assay of liberated ammonia from the substrate utilizing the Caraway's colorimetry. The extraction of both DNA and RNA was performed by the Schmidt-Thannhauser's method, which was followed by Marmur's method of purification for DNA and by Chargaff's method of purification for RNA. The determinations of both DNA and RNA were carried out by the diphenylamine reaction for the former and by the orcinol reaction for the latter. The following resume was the results of the present work. 1. It was observed that the body as well as liver weights fall abruptly during starvation, and that the loss of body weight showed no statistical correlation with the decreases in the content of liver protein. 2. The content of liver protein and activity of liver guanine deaminase activity as well decline dramatically, and the specific activities of the enzyme (activity/protein), however, decreased gradually as starvation proceeded. 3. Both of the nucleic acids, DNA and RNA, showed decrements in the liver of mouse during acute starvation; the latter, however, being more striking in the decline as compared to the former. 4. The decreases in the liver protein content as resulted from the acute starvation had no statistically significant correlation with the decrements of DNA in the same tissue, but had regressed with a significant statistical correlation with the fall of RNA in the tissue. 5. The decrease in the activity of guanine deaminase in the liver of mouse during acute starvation was functionally more proportional to the decrease in RNA than DNA, and moreover correlated with the changes in the content of the liver protein. 6. The possible mechanisms involved during in this acute starvation as bring the decreases in the contents of DNA, protein, and guanine deaminase were discussed briefly.

• 대한지역사회영양학회지
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• 제15권4호
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• pp.460-474
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• 2010

This study was performed to assess the physical activity, food habit and nutrient intakes by gender and age groups in 193 adults aged 20-59 years (84 men and 109 women) in Pusan. Data for physical activity and dietary survey was assessed by a questionnaire and 24hr recall method. The mean BMIs of men and women were 24.0 and 22.2 respectively and BMI of women in the 20-29 years group (20.9) was significantly lower than that of women in the 30-49 and 50-59 years group (22.7, 23.2) (p < 0.01). 56.0% for men and 44.0% for women exercised regularly. The mean exercise duration per once of men (69.7 minutes) was significantly higher than that of women (52.4minutes) (p < 0.01). The mean exercise duration per day was 36.0 minutes for men and 29.9 minutes for women. 67.9% for men and 78.0% for women often skipped meals and 68.4% for men and 69.4% for women skipped breakfast in the main. The mean energy intake of men was 2067.2 kcal and that of women was 1783.1 kcal comprised of 87.2% and 92.1% of the Estimated Energy Requirements (EER). The mineral intakes of men and women were over Recommended Intake (RI) and Adequate Intake (AI) except calcium and potassium. The mean calcium intake was 88.3% for men and 84.0% for women of RI. The mean potassium intake was 63.3% for men and 59.2% for women of AI. The mean vitamin intakes of men and women were over RI and AI except vitamin C and folic acid. The mean vitamin C intake was 92.5% for men and 85.6% for women of RI. The mean folic acid intake was 76.6% for men and 70.0% for women of RI. The mean energy, protein, sodium and zinc intakes of men were significantly higher than those of women (p < 0.01, p < 0.05, p < 0.05, p < 0.05). The mean vitamin $B_6$, vitamin C and folic acid intakes of men in the 20-29 years group were significantly lower than those of men in the 30-49 and 50-59 years group (p < 0.01, p < 0.05, p < 0.05). For energy, proportions of subjects with intake levels less than 90% EER were 64.3% for men and 56.0% for women. For calcium, proportions of subjects with intake levels less than Estimated Average Requirement (EAR) were 52.4% for men and 59.6% for women. For folic acid, proportions of subjects with intake levels less than EAR were 78.6% for men and 83.5% for women. For iron and phosphorus, proportions of women (36.7%, 14.7%)with intake levels less than EAR were significantly higher than those of men (6.0%, 1.2%) (p < 0.01, p < 0.001). For men, age was positively correlated with intakes of potassium, vitamin $B_6$, vitamin C and folic acid (p < 0.05, p < 0.05, p < 0.05, p < 0.01). For men, weight showed significantly negative correlations with intakes of carbohydrate, phosphorus, potassium, zinc, vitamin $B_6$ and folic acid (p < 0.01, p < 0.05, p < 0.05, p < 0.05, p < 0.01) and BMI showed significantly negative correlations with protein, lipid, phosphorus, potassium, zinc, vitamin E and folic acid (p < 0.05, p < 0.05, p < 0.05, p < 0.05, p < 0.05, p < 0.05, p < 0.05) For men, exercise duration per once showed significantly positive correlations with intakes of calcium, phosphorus, potassium, zinc, vitamin $B_1$, vitamin $B_2$, niacin, vitamin C and folic acid (p < 0.01, p < 0.05, p < 0.01, p < 0.05, p < 0.05, p < 0.01, p < 0.01, p < 0.01, p < 0.05). Therefore, nutritional education for adult health management is needed by gender and age groups.

• Journal of Nutrition and Health
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• 제47권1호
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• pp.77-88
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• 2014

본 연구는 기숙사 생활을 하는 제주지역 고등학생을 대상으로 기숙사급식 만족도와 급식에서 제공되는 메뉴에 대한 기호도를 조사 분석함으로써 기숙사 학생들의 다양한 요구와 필요에 부합하고 기숙사 급식의 질적 향상을 모색하기 위한 기초자료로 활용하고자 실시하였다. 조사대상 고등학생의 성별은 남자가 71.1%, 학년은 2학년 46.0%, 기숙사 거주기간이 2~3학기인 학생이 45.3%, 월평균 용돈은 3만원 미만이 39.0%, 동지역에 거주하는 학생이 68.3%로 나타났다. 방학 중 외식횟수는 한 달에 1~2회가 51.4%, 부모가 맞벌이를 하는 학생은 73.7%, 어머니의 학력은 고졸이 46.9%, 본인이 건강하다고 생각하는 학생은 60.3%로 나타났다. 조사대상의 기숙사급식 만족도에 있어서는 메뉴 품질에 대한 만족도 (3.26)보다 서비스 및 위생에 대한 만족도 (3.46)가 높았고, 메뉴 품질에 있어서는 주식 (밥)의 양 (3.70), 음식의 온도 (3.50), 음식의 신선도 (3.42), 음식의 맛 (3.14)의 순으로, 서비스 및 위생에 있어서는 급식 정보 제공 (3.66), 식당 분위기(3.53), 급식 위생 (3.48), 식사질서 지도 (3.43), 원활한 배식 (3.34), 조리종사자의 친절도 (3.33)의 순으로 나타났다. 조사대상 고등학생의 끼니별 급식 만족도는 저녁식사 (3.70), 점심식사 (3.50), 아침식사 (3.14)의 순으로 나타났으며, 성별에 있어서는 아침식사의 경우 여학생의 만족도 (3.36)가 남학생의 만족도 (2.93)보다 유의적으로 높게 나타났다 (p < .01). 조사대상이 기숙사급식에서 제공받은 음식의 섭취량을 끼니별로 분석한 결과 저녁식사 (3.70), 점심식사 (3.50), 아침식사 (3.14)의 순으로 나타났고 점심식사와 저녁식사는 남학생의 섭취량 (각 4.12, 4.17)이 여학생 (각 3.72, 3.76) 보다 유의적으로 많았다 (p < .001). 조사대상 고등학생의 기숙사급식에 대한 개선점에 있어서는 맛의 향상 (43.4%), 메뉴의 다양성 (31.0%), 배식량 증가 (17.4%), 급식위생 (3.8%), 급식환경 (3.3%)의 순으로 응답율을 보였고 남학생의 경우 맛의 향상 (49.2%), 메뉴의 다양성 (28.7%), 배식량 증가 (14.9%), 급식환경 (4.6%), 급식위생 (1.3%)의 순으로, 여학생은 메뉴의 다양성 (36.6%), 맛의 향상 (29.3%), 배식량 증가 (23.6%), 급식위생 (8.1%), 급식환경 (1.6%)의 순으로 나타났다 (p < .001). 조사대상의 주식류에 대한 기호도는 면류 (4.06), 일품음식류 (3.92), 밥류 (3.66)의 순으로 높게 나타났고 밥류에 있어서는 검정쌀밥 (3.80), 일품음식류는 볶음밥 (4.05), 면류에 있어서는 스파게티 (4.21)의 기호도가 가장 높게 나타났다. 국 및 찌개류에 대한 기호도는 갈비탕 (4.10), 김치찌개 (3.99), 된장찌개 (3.92), 순두부찌개 (3.79), 육개장 (3.78)의 순으로 나타났고 동태찌개 (2.72)와 북어국 (2.61)은 보통 이하의 기호도를 보였다. 조사대상의 부식류에 대한 기호도에 있어서는 찜류 (3.95), 튀김류 (3.87), 볶음류 (3.57), 김치류 (3.54), 구이류 (3.49), 조림류 (3.27)의 순으로 나타났고 무침류는 3점 미만인 2.89를 나타내 보통 이하의 기호도를 보였다. 메뉴 아이템에 있어서는 갈비찜 (4.24), 닭튀김 (4.23), 닭볶음 (4.19), 돈까스 (4.17), 탕수육 (4.10), 돼지고기볶음 (4.04)의 순으로 높은 기호도를 보였고, 5점 만점에 대해 4점 이상을 나타냈으며, 콩조림 (2.94), 시금치무침 (2.94), 애호박무침 (2.89), 느타리버섯볶음 (2.84), 생선구이 (2.81), 마늘쫑무침 (2.69), 취나물무침 (2.67), 청경채 겉절이 (2.71), 톳무침 (2.65), 생선조림 (2.63), 건파래 무침 (2.59)은 5점 만점에 대해 3점 미만을 나타내 보통 이하의 기호도를 보여 생선이나 채소류보다 육류를 이용한 음식에 대한 기호도가 높음을 알 수 있었다. 후식류에 있어서는 쥬스류의 기호도 (4.52)가 가장 높았고 요구르트 (4.50), 생과일 (4.42), 빵류 (4.33), 우유 (4.08), 떡류 (4.01)의 순으로 나타났으며 빵류 (p < .05) 와 떡류 (p < .01)에 대해 남학생보다 여학생의 기호도가 유의적으로 높게 나타났다. 조사대상의 메뉴 기호도는 기숙사급식 만족도 (r = 0.369), 메뉴 품질 (r = 0.348), 서비스 및 위생 (r = 0.344), 끼니별 급식 만족도 (r = 0.418), 제공된 음식 섭취량 (r = 0.220)과 정 (+)의 상관관계를 (p < .01), 기숙사급식 만족도는 메뉴품질 (r = 0.944), 서비스 및 위생 (r = 0.931), 끼니별 급식 만족도 (r = 0.707)와 다소 높은 정 (+)의 상관관계를 (p < .01), 메뉴품질은 서비스 및 위생 (r = 0.758), 끼니별 만족도 (r = 0.717)와 다소 높은 정 (+)의 상관관계를 (p < .01), 서비스 및 위생은 끼니별 급식 만족도 (r = 0.604)와 정 (+)의 상관관계를 나타냈다 (p < .01). 이상의 연구결과를 토대로 살펴볼 때, 기숙사급식에의 의존도가 높은 기숙사생들이 영양적으로 균형 있는 음식을 섭취하고 올바른 식습관을 형성할 수 있도록 다각적이고 체계적인 영양교육이 병행되어야 하며 배식량 및 배식방법 등을 개선하고 다양한 조리법 및 이벤트 메뉴 등을 개발하는 등 학생들이 성장기에 필요한 영양소를 충분히 섭취할 수 있도록 다양한 방안이 모색되어야 할 것으로 사료된다.

• Asian-Australasian Journal of Animal Sciences
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• 제26권6호
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• pp.845-855
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• 2013

Requirements of dietary chloride (dCl) and chloride salts were determined by using $4{\times}2$ factorial arrangement under four phase feeding program. Four levels (0.31, 0.45, 0.59 and 0.73%) and two sources ($NH_4Cl$ and $CaCl_2$) of the dCl were allocated to 1,472 chicks in eight dietary treatments in which each treatment was replicated four times with 46 birds per replicate. The four phase feeding program was comprised of four dietary phases: Prestarter (d 1 to 10), Starter (d 11 to 20), Grower (d 21 to 33) and Finisher (d 34 to 42); and diets were separately prepared for each phase. The cations, anions, pH, dissolved oxygen (DO), temperature, electrical conductivity (EC), total dissolved solids (TDS) and salinity were analyzed in drinking water and were not affected by dietary treatments. BW gain (BWG; $p{\leq}0.009$) and feed:gain (FG; $p{\leq}0.03$) were improved in $CaCl_2$ supplemented diets during d 1 to 10. The maximum response of BWG and FG was observed at 0.38% and 0.42% dCl, respectively, for d 34 to 42. However, the level of dCl for BWG during d 21 to 33 ($p{\leq}0.04$) and d 34 to 42 ($p{\leq}0.009$) was optimized at 0.60% and 0.42%, respectively. The level of dCl for optimized feed intake (FI; $p{\leq}0.006$), FG ($p{\leq}0.007$) and litter moisture (LM; $p{\leq}0.001$) was observed at 0.60%, 0.38% and 0.73%, respectively, for d 1 to 42. Water intake (DWI) was not affected by increasing dCl supplementation (p>0.05); however, the ratio between DWI and FI (DWI:FI) was found highest at 0.73% dCl during d 1 to 10 ($p{\leq}0.05$) and d 21 to 33 ($p{\leq}0.009$). Except for d 34 to 42 ($p{\leq}0.006$), the increasing level of dCl did not result in a significant difference in mortality during any phase. Blood pH and glucose, and breast and thigh weights (percentage of dressed weight) were improved while dressing percentage (DP) and gastrointestinal health were exacerbated with $NH_4Cl$ as compared to $CaCl_2$ supplemented diets ($p{\leq}0.001$). Higher plasma $Na^+$ and $HCO_3{^-}$ and lower $Cl^-$ and $Ca^{{+}{+}}$ were observed in $NH_4Cl$ supplemented diets ($p{\leq}0.001$). Increasing supplementation of dCl increased plasma $Cl^-$ ($p{\leq}0.04$; quadratically) and linearly reduced plasma $K^+$ ($p{\leq}0.001$), $Ca^{{+}{+}}$ ($p{\leq}0.003$), $HCO_3{^-}$ ($p{\leq}0.001$), and $Na^+$ ($p{\leq}0.001$; quadratically). Consequently, higher requirements of dietary chloride are suggested for feed intake; nevertheless, lower levels of dietary chloride are sufficient to support optimal BWG and FG with increasing age. The $NH_4Cl$ supplemented diets ameliorate breast and thigh meat yield along with overall energy balance (glucose).

• 한국수산과학회지
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• 제28권6호
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• pp.812-813
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• 1995

Lactococcal cells are nutritionally fastidious and thus, generally cultured either in milk or M17 medium (Terzaghi and Sandine, 1975). In this study, Lactococcus cremoris wild-type (KH) and its less­proteolytic mutant (KHA1) cells were grown on the M17 medium or with modified M17 medium by replicated parallel experiments. The modified M17 medium had the same composition as M17 medium, except that lactose was replaced by glucose. Analyses of culture-broth samples, in which the M17 and the modified M17 media were used, were conducted by high-performance liquid chromatography (HPLC). But, working with these media created noisy problems in analyses of samples. Therefore, a new semi-synthetic medium was developed on the basis of nutritional requirements (Morishita et al., 1981). The composition of the semi-synthetic medium determined on the basis of the nutritional requirements and the composition of milk, is presented in Table 1. The composition of M17 medium is also presented and compared in the table. L. cremoris KH and KHA1 cells were grown again on the new synthetic medium containing glucose or lactose. The broth samples were then drawn and analyzed by HPLC. Clearer separations of fermented products were achieved from the new medium than those with the M17 and the modified M17 media. In comparison with the M17 or the modified M17 media, growth on the new medium was good (Kim et al, 1993). Additional fermentations were also carried out at a controlled pH of 7.0, where enhanced growth of lactococcal cells was obtained. In the fermentations, samples were also analyzed for the concentrations of sugar and lactic acid. The results showed that the new synthetic medium was as good as or better than the M 17 and the modified M 17 media. This is because casein hydrolysate in the synthetic medium provided a ready supply of amino acids and peptides for L. cremoris KH and KHA1 cells. Lactic acid bacteria (LAB) including Lactococcal cells have been known to be an effective means of preserving foods, at the same time as giving particular tastes in fields of dairy products. LAB also have always occupied an important place in the technology of sea products, and marine LAB have known to be present in traditional fermented products (Ohhira et al, 1988). To apply the new synthetic medium to marine LAB, two different LAB were isolated from pickled anchovy and pollacks caviar and were grown on the new media in which various concentrations of NaCl $(3, 5, 7 and 10\%)$ added. They were also grown on the medium solution in natural seawater $(35\%o\;salinity)$ and on the solution of natural seawater itself, too. As seen in Fig. 1, Marine LAB were grown best on the synthetic medium solution in natural seawater and the higher concentrations of NaCl were added to the medium, the longer lag-phase of growth profile appeared. Marine LAB in natural seawater were not grown well. From these results, the synthetic medium seems good to cultivate cells which are essential to get salted fish aged. In this study, it showed that the new synthetic medium provided adequate nutrition for L. cremoris KH and KHA1 cells, which have been used as cheese starters (Stadhouders et al, 1988). Using this new medium, the acid production capability of starter cultures could be also measured quantitatively. Thus, this new medium was inferior to the M17 or the modified M17 medium in culturing the cheese starters and in measuring fermentation characteristics of the starter cells. Moreover, this new medium found to be good for selected and well-identified marine LAB which are used in rapid fermentations of low-salted fish.

• Asian-Australasian Journal of Animal Sciences
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• 제14권5호
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• pp.720-732
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• 2001

Skeletal muscle is of major economic importance since it is finally converted to meat for consumers. The increase in meat production with low costs of production may be achieved by optimizing muscle growth, whereas a high meat quality requires, among other factors, the optimization of intramuscular glycogen and fat stores. Thus, research in energy metabolism aims at controling muscle metabolism, but also liver and adipose tissue metabolism in order to optimize energy partitioning in favour of muscles. Liver is characterized by high anabolic and catabolic rates. Metabolic enzymes are regulated by nutrients through short-term regulation of their activities and long-term regulation of expression of their genes. Consequences of liver metabolic regulation on energy supply to muscles may affect protein deposition (and hence growth) as well as intramuscular energy stores. Adipose tissues are important body reserves of triglycerides, which result from the balance between lipogenesis and lipolysis. Both processes depend on the feeding level and on the nature of nutrients, which indirectly affect energy delivery to muscles. In muscles, the regulation of rate-limiting nutrient transporters, of metabolic enzyme activities and of ATP production, as well as the interactions between nutrients affect free energy availability for muscle growth and modify muscle metabolic characteristics which determine meat quality. The growth of tissues and organs, the number and the characteristics of muscle fibers depend, for a great part, on early events during the fetal life. They include variations in quantitative and qualitative nutrient supply to the fetus, and hence in maternal nutrition. During the postnatal life, muscle growth and characteristics are affected by the age and the genetic type of the animals, the feeding level and the diet composition. The latter determines the nature of available nutrients and the rate of nutrient delivery to tissues, thereby regulating metabolism. Physical activity at pasture also favours the orientation of muscle metabolism, towards the oxidative type. Consequently, breeding systems may be of a great importance during the postnatal life. Research is now directed towards the determination of individual tissue and organ energy requirements, a better knowledge of nutrient partitioning between and within organs and tissues. The discovery of new molecules (e. g. leptin), of new molecular mechanisms and of more powerful techniques (DNA chips) will help to achieve these objectives. The integration of the different levels of knowledge will finally allow scientists to formulate new types of diets adapted to sustain a production of high quality meat with lower costs of production.

• 한국식생활문화학회지
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• 제35권4호
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• pp.333-341
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• 2020

This study evaluated the perception, consumption behavior, and optional attributes of Korean, Chinese, and U.S. consumers of energy bar products. Data were compared and analyzed by surveying 300 consumers in each country. Significant differences were observed in preference for energy bars according to their nationality, in the order China, the U.S., and Korea. Perception of taste, types and dietary suitability of the products ranked lower for Korean consumers, as compared to consumers of the United States and China. The order dietary fiber, protein, and calcium were the sought-after nutritional requirements of the products. The demand for protein was significantly higher in the U.S. Calcium demand was low in the United States and China, but was very high in Korea, which could be attributed to the low calcium intake of Koreans. Other optional attributes which were closely associated with the purchase and re-purchase decision, included price, taste and delivery period. All three factors were recognized as important options in Korea, whereas awareness of packaging/appearance and brand was not. The taste, nutrients and price in the U.S. ranked high as important optional attributes, while the packaging, external and expiration dates were recognized as low. Unlike Korea and the U.S., important optional attributes for Chinese consumers were determined in the order expiration date, taste, and nutrients, and showed low perception for packaging, appearance, weight, counts, and prices. Evaluating the preference for the main and secondary ingredients, Koreans preferred nuts over grains, Americans preferred dried fruits over nuts, and Chinese preferred nuts and grains; both Korean and American consumers had low preference for dried vegetables. The preference for chocolate was low in Korea and China, whereas preference for jelly was high in China as compared to Korea and the U.S. The intention of purchasing energy bars was significantly lower in Korea than in the U.S. and China. A variety of nutritious functional bars have recently been distributed and sold in Korea, but they are mostly produced in the U.S., which is the largest producer and consumer worldwide. Taken together, results of this study indicate that the demand for nutritional enhancement and preferred materials vary according to the nationality. Hence, it is necessary to develop products that reflect these criteria. Further research is required to analyze the relationship between preference and consumption behavior for each material product developed in the future.