• Asian-Australasian Journal of Animal Sciences
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• 제23권4호
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• pp.491-500
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• 2010

Three experiments were conducted to investigate the effects of polyurethane coated urea on in vitro ruminal fermentation, ammonia release dynamics and lactating performance of Holstein dairy cows fed a steam-flaked corn-based diet. In Exp. 1, a dual-flow continuous culture was run to investigate the effect of polyurethane coated urea on nutrient digestibility, rumen fermentation parameters and microbial efficiency. Three treatment diets with isonitrogenous contents (13.0% CP) were prepared: i) feedgrade urea (FGU) diet; ii) polyurethane coated urea (PCU) diet; and iii) isolated soy protein (ISP) diet. Each of the diets consisted of 40% steam-flaked corn meal, 58.5% forages and 1.5% different sources of nitrogen. PCU and FGU diets had significantly lower digestibility of NDF and ADF (p<0.01) than the ISP diet. Nitrogen source had no significant effect (p = 0.62) on CP digestibility. The microbial efficiency (expressed as grams of microbial N/kg organic matter truly digested (OMTD)) in vitro of the PCU diet (13.0 g N/kg OMTD) was significantly higher than the FGU diet (11.3 g N/kg OMTD), but comparable with the ISP diet (14.7 g N/kg OMTD). Exp. 2, an in vitro ruminal fermentation experiment, was conducted to determine the ammonia release dynamics during an 8 h ruminal fermentation. Three treatment diets were based on steam-flaked corn diets commonly fed to lactating cows in China, in which FGU, PCU or soybean meal (SBM) was added to provide 10% of total dietary N. In vitro $NH_3-N$ concentrations were lower (p<0.05) for the PCU diet than the FGU diet, but similar to that for the SBM diet at all time points. In Exp. 3, a lactation trial was performed using 24 lactating Holstein cows to compare the lactating performance and blood urea nitrogen (BUN) concentrations when cows were fed PCU, FGU and SBM diets. Cows consuming the PCU diet had approximately 12.8% more (p = 0.02) dietary dry matter intake than those consuming the FGU diet. Cows fed the PCU diet had higher milk protein content (3.16% vs. 2.94%) and lower milk urea nitrogen (MUN) concentration (13.0 mg/dl vs. 14.4 mg/dl) than those fed the FGU diet. Blood urea nitrogen (BUN) concentration was significantly lower for cows fed the PCU (16.7 mg/dl) and SBM (16.4 mg/dl) diets than the FGU (18.7 mg/dl) diet. Cows fed the PCU diet had less surplus ruminal N than those fed the FGU diet and produced a comparable lactation performance to the SBM diet, suggesting that polyurethane coated urea can partially substitute soybean meal in the dairy cow diet without impairing lactation performance.

• 한국농림기상학회지
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• 제20권4호
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• pp.357-365
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• 2018

본 연구는 울산광역시 봉대산 산불 훼손 복원지를 대상으로 토양개량제 처리가 토양층에 매설된 wood stakes의 분해율과 유기 탄소 및 질소 동태에 미치는 영향을 조사하기 위하여 실시하였다. 산불 훼손 복원지에 식재된 튤립나무, 상수리나무, 왕벚나무, 곰솔 조림지와 미복원지를 대상으로 2015년 3월 토양 0~15cm 깊이에 wood stakes를 매설한 후, 2년 동안 각 1회씩 토양개량제(CLB: 복합비료 + 석회 + 목탄 처리구; LB: 석회 + 목탄 처리구)를 처리하고, 2015년 10월, 2016년 3월, 2016년 10월 채취하여 분해율과 유기탄소 및 질소 농도를 조사하였다. Wood stakes 분해율의 경우 토양개량제 처리구와 대조구 사이에 유의적인 차이는 없었으나, 유기 탄소 잔존율의 경우 대조구(43.7%), CLB처리구(71.3%), LB처리구(71.6%) 순으로 토양개량제 처리구의 탄소 무기화가 대조구에 비해 낮게 나타났다. Wood stakes 내 질소 잔존율의 경우도 대조구가 29.7%로 LB처리구 52.6%에 비해 낮아 탄소 무기화율과 유사한 경향을 보였다. 본 연구결과에 따르면 산불 훼손 복원지에 토양개량제 처리는 wood stakes의 탄소 및 질소 무기화를 지연하는 것으로 나타났다.

• Asian Journal of Atmospheric Environment
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• 제5권3호
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• pp.172-180
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• 2011

The transfer of microorganisms is important process for ecosystems. Microorganisms in dryland can transport itself to wetland through atmospheric diffusion, but only few papers reported about the atmospheric bioaerosol present over dryland. We carried out the direct sampling using a tethered balloon over Dunhuang City, China's northwestern dryland. Bioaerosols were collected using a tethered balloon with a bioaerosol collector at 820 m above the ground (1,960 m above the sea level) around noon on August 17, 2007. The bioaerosols were cultured after the collection at Dunhuang Meteorological observatory. Two strains of molds were isolated using the Nutrient agar medium. About 400-bp 18S rRNA partial sequences were amplified by PCR and determined afterwards. The results of a homology search by 18S rRNA sequences of isolates in DNA databases (GenBank, DDBJ, and EMBL) and an observation of the form revealed that two bioaerosols in the convective mixed layer over Dunhuang City were Cladosporium sp. and Aspergillus sp.

• Journal of Ecology and Environment
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• 제37권1호
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• pp.13-19
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• 2014

The effect of different turnover time of water on the decomposition of emergent macrophyte litter (Zizania latifolia Griseb.) was investigated using a microcosm experiment. Microcosm treatment represented different turnover time of water; 1, 2, 4 and 8 weeks. The litterbags from each treatment were retrieved every 2 weeks until the 8th week and the water simultaneously sampled with the litterbag. The dry weight and the content of major cations in the litter, and the content of available N, P, and major cations in the water were analyzed. Dry weight loss after 8 weeks indicated the lower decay rates under the condition of short turnover time of water. Major cations from the litter and the water showed that the leached amounts of K and Mg from the litter were highest in the 2nd week and dramatically decreased from the 4th week. The dynamics of available nitrogen and phosphorus in the water showed that as the water turnover time was getting longer, the amounts of available nitrogen and phosphorus remained higher. These results suggest that wetlands with longer turnover time of water could maintain the increased nitrogen and phosphorus and no outflow of the nutrients could cause eutrophication problem.

• 한국환경과학회지
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• 제9권2호
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• pp.115-123
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• 2000

The dynamics of water quality and phytoplankton population had examined by monthly sampling from the upper to the lower part of watergate in an artificial Shihwa Reservoir in which situated near newly cities and incustrial complex on the west coast of Korea from January 1997 to December 1998. Among environmental factors, yearly average concentration of chl-a, TN and TP seemed to eutrophic or hypertrophic conditions that ranged 146.4~245.8 $\mu\textrm{g}$/$\ell$, 1.6~2.7 mg N/$\ell$, 258~448 $\mu\textrm{g}$ P/$\ell$, 26.9~80.7 $\mu\textrm{g}$/$\ell$, 1.0~2.4 mgN/$\ell$ and 74~239 $\mu\textrm{g}$P/$\ell$ respectively. Water quality was extremely deteriorated to consistently accumulation into inner reservoir by load of pollutants from autochthonous and allochthonous until early July 1997 after embankment. Water pollution of Shihwa Reservoir was remarkble on the biological condition with largely persistent bloom of phytoplankton and increase rate of standing crops was 2.4/yr. The development trend of phytoplankton in water ecosystem were closely related to increse and decrease of physico-chemical factors and those scale seemed to control by nutrient contents. Inflow of seawater into reservoir to object of repair of water quality. As to see dominant species, composition of those composed to mostly freshwater algae before inflow of seawater such as Selenastrum capricornutum of green algae, cyclotella atomus, C. meneghiniana of diatom and Microcystis spp. of blue-green algae and the other hand brackish algae were dominated after inflow of seawater such as Chaetoceros dicipiens, Skeletonema costatum of diatom, Dinophysis acuminata, Gymnodinium mikimotoi, G. sanguineum, Gyrodinium spirale, Prorocentrum minmum of dinoflagellate and Eutreptiella gymnastica of euglenoid. Moreover, small flagellates including Chroomonas spp. of cryptomonad were abundant throughout the year. The cause of water deterioration during fill of the freshwater were complexly supported with extra and intra parameters. The variation pattern of phytoplankton were related to water temperature and salinity by inflow of seawater based to plentiful nutrients. The dynamics of phytoplankton were assessed to ecosystem that clearly condition of dominant by unique or a few angel species seasonally.

• ALGAE
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• 제27권3호
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• pp.215-224
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• 2012

Growth dynamics of the seagrass, Zostera marina were examined at the two stations (Myungju and Dagu) in Jindong Bay on the southern coast of Korea. Eelgrass leaf productivities, underwater irradiance, water temperature, dissolved inorganic nitrogen (DIN) in water column and sediments, and tissue carbon (C) and nitrogen (N) content were monitored monthly from March 2002 to January 2004. Underwater irradiance fluctuated highly without a clear seasonal trend, whereas water temperature showed a distinct seasonal trend at both study stations. Water column DIN concentrations were usually less than $5{\mu}M$ at both study sites. Sediment pore water $NH_4{^+}$ and $NO_3{^-}+NO_2{^-}$ concentrations were higher at the Myungju site than at the Dagu site. Eelgrass leaf productivity at both study sites exhibited a distinct seasonality, increasing during spring and decreasing during summer. Seasonal variation of eelgrass productivity was not consistent with seasonal patterns of underwater irradiance, or water temperature. Eelgrass tissue C and N content at both study sites also showed significant seasonal variations. Relationships between tissue C and N content and leaf productivities exhibited usually negative correlations at both study sites. These negative correlations implied that the growth of Z. marina at the study sites was probably limited by C and N supplies during the high growth periods.

• Journal of Microbiology and Biotechnology
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• 제13권5호
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• pp.641-646
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• 2003

An accurate and efficient method for measuring the mass of hairy roots using conductometry is established. A conductivity equation expressed in terms of the concentration of the ion species in the medium is suggested. By using this equation, the effect of the individual ions on the total conductivity can be quantitatively analyzed. An equation for the in situ estimation of the cell growth coefficient for determining the mass of hairy roots is established based on measurements of the nitrogen concentration and conductivity during cultivation. The proposed equation does not require preliminary experiments to determine the cell growth coefficient. Instead, the physiological characteristics of the plant species are reflected by introducing the cellular nitrogen content. Since the cell growth coefficient is determined by measuring the major ionic nutrient concentrations, it is more effective to express the dynamics of an actual culture system. This improved method for determining the mass of hairy roots was successfully utilized in a fed-batch culture system.

• Animal cells and systems
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• 제2권3호
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• pp.333-339
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• 1998

Litter decomposition is a key process in energy flow and nutrient cycling in the freshwater littoral zone, and is regulated by physicochemical properties of litters. Using a litterbag method, we compared the decomposition rates of 16 different litter types from 10 plant species of the emergent macrophytes for one year in the littoral zone of the Paltangho Reservoir, Korea. The regression analysis fitted to the various decomposition models showed that mass loss of the litters with time best fitted an asymptotic function. The litters of the emergent macrophytes were composed of two compartments, labile and refractory. The macrophytic litters showed a great variety in decomposition dynamics depending on sources of litters. The labile compartment of the initial litter mass was in a wide range between 18% and 99%, and their decomposition rates varied from 0.0037 to 0.0131 day-1. The decomposition processes of the emergent macrophytes were determined by the relative amounts of the labile and refractory compartments and by the decomposition rate of the habile one in the littoral zone.

• 한국산업융합학회 논문집
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• 제5권4호
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• pp.347-351
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• 2002

유입되는 담수의 영향을 크게 받는 한 하구 (미국 버지니아주의 요크강)의 크기별 식물성플랑크통의 역학에 대한 수리역학적 조절에 대한 조사를 위해 플랑크톤 생태계모델이 계발되었다. 모델은 요크강 하류의 상부층에서의 탄소 및 영양염의 분포를 나타내는 12개의 구성체 변수로 이루어져 있다. 빛, 온도, 바람, 해류 그리고 조수와 같이 이송과 혼합을 일으키는 물리적 변수들로 포함하고 있다. 모델은 포트란으로 계발되었고 Runge- Kutta technique을 이용해 미분방정식을 풀었다. 모델분석 결과, 크기가 큰 마이크로 식물성플랑크톤의 수화는 수체내의 생산보다는 이송이나 확산 때문에 나타나는 것임을 알 수 있었다.

• 한국산림과학회지
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• 제89권1호
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• pp.41-48
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• 2000

1992년 10월부터 1995년 11월까지 38개월 동안 경기도 광릉 시험림에서 낙엽활엽수(졸참나무, 서어나무, 까치박달 혼합)와 3종의 침엽수 낙엽의 분해율과 분해과정에 따른 영양염류 함량의 변화를 조사하였다. 38개월이 지난 후 혼합 활엽수, 잣나무, 리기다소나무 그리고 전나무 낙엽의 잔존률은 각각 16.2%, 29.8%, 33.5%, 53.9%로 활엽수, 소나무속(屬) 그리고 전나무 순으로 분해율이 감소하였으며, k는 각각 0.61, 0.40, 0.37, $0.21yr^{-1}$이었다. 전나무 낙엽의 초기 질소함량은 6.5mg/g으로 다른 종류의 낙엽에 비해 1/2 수준이었으며, 전나무 낙엽의 분해율이 낮은 것은 그것의 질소함량이 낮기 때문인 것으로 판단된다. 38개월이 경과된 낙엽에서 질소의 잔존률은 활엽수 낙엽에서 29%인데 비해 전나무 낙엽은 107.8%이었다. 인의 잔존률은 활엽수 낙엽에서 53.4%이었는데 비해 침엽수 낙엽은 모두 초기 값 이상이었다. 양이온의 잔존률은 38개월 경과된 낙엽에서 모두 초기 값 이하이었다. 분해과정에 따른 영양염류 함량의 변화 및 잔존량은 낙엽과 영양염류의 종류에 따라 차이가 있었다.