• 제목/요약/키워드: nutrient culture

검색결과 804건 처리시간 0.031초

경제적인 자동화 FIA 장치를 이용한 진탕플라스크 배양액의 미생물 증식도의 자동측정 (Automatic Measurement of Microbial Growth in Snake-Flask Culture using an Economic and Automated flow injection Analysis Apparatus)

  • 이형춘
    • KSBB Journal
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    • 제19권3호
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    • pp.241-244
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    • 2004
  • 진탕플라스크 배양액의 균 증식도를 자동측정하기 위하여 자동화 FIA장치를 경제적으로 제작하였으며, 이를 이용하여 대장균을 Nutrient broth 배지에 접종하여 배양하면서 그 증식도를 자동측정하였다. 멸균 NB를 이송용액으로 하고 10분 간격으로 자동측정하였을 때 큰 오차 없이 증식양상을 세밀하게 측정할 수 있었다. 증류수를 이송용액으로 사용한 경우에는 NB를 이송용액으로 사용한 경우에 비하여 측정오차가 더 컸으며, 특히 배양후반부에서의 오차가 크게 나타났다.

Effect of Nutrient Nitrogen on the Degradation of Pentachlorophenol by White Rot Fungus, Phanerochaete chrysosporium

  • Chung, Nam-Hyun;Kang, Gu-Young;Kim, Gyu-Hyeok;Lee, Il-Seok;Bang, Won-Gi
    • Journal of Microbiology and Biotechnology
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    • 제11권4호
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    • pp.704-708
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    • 2001
  • The effect of nutrient nitrogen on the degradation of pentachlorophenol (PCP) by Phanerochaete chrysosporium in a liquid culture was investigated. PCP disappeared at almost the same rate in both nutrient nitrogen-sufficient (NS) and -limited (NL) sttionary cultures. However, more pentachloroanisole (PCA) was accumulated in the NS culture than in the NL culture. The effect of nitrogen on the degradation of PCA was also tested in both cultures. PCA disappeared faster in the NL culture than in the NS culture, indicating that the lower accumulation of PCA during the degradation of PCP in the NL culture was due to the faster degradation of PCA in the NL culture than in the NS culture. In another experiment, PCA was added to shaking cultures rather than stationary cultures to search for any other metabolite(s). While no other metabolite but PCA was found in the NS stationary culture, 2,4,5,6-tetrachloro-2,5-cyclohexadiene-1,4-dione(TCHD) was found as the only indentifiable product in the NL shaking culture. Thus, PCP would appear to be metabolized to TCHD via PCa or directly oxidized to TCHD by lignin peroxidase. Since all the above results indicate that no innocuous metabolite was formed during the degradation of PCP by the fungus, it is quite feasible to use the fungus in the biotreatment of PCP.

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직파(直播) 벼의 제초제(除草劑) 약해(藥害) 요인(要因)과 회복(回復) 연구(硏究) - 2. 영양(營養) 조건(條件)에 따른 약해(藥害) (Factors and Recovery of Herbicide Phytotoxicity on Direct-seeded Rice - 2. Phytotoxicity of Herbicide with Nutrient Condition)

  • 임일빈;구정건이
    • 한국잡초학회지
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    • 제17권1호
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    • pp.24-30
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    • 1997
  • 담수 직파 벼에서 최근 많이 사용하고 있는 제초제들의 영양(營養) 유무(有無)에 따른 약해 반응을 검토한 결과는 다음과 같다. 1. Bensulfuron-methyl, pyrazosulfuron-ethyl 및 imazosulfuron의 처리는 경엽부(莖葉部)보다 근부(根部)의 생육을 크게 억제시켰으며 영양(營養) 조건 재배에서 무영양(無營養) 조건의 재배에서보다 생육 억제 정도가 컸다. 또한 영양(營養) 재배와 무영양(無營養) 재배에서 벼 생육 억제율(抑制率)의 차이는 pyrazosulfuronethyl의 처리에서 가장 컸다. 2. Dimepiperate의 처리는 경엽부(莖葉部) 근부(根部) 모두 무영양(無營養) 조건에서 생육 저해 정도가 컸으며, 근부(根部)보다 경엽부(莖葉部)의 생육을 크게 억제시켰으며, 특히 초장신장이 크게 억제되었다. 3. Molinate 처리는 경엽부(莖葉部)의 억제율(抑制率)이 근부(根部)보다 컸으며 경엽부(莖葉部)는 영양(營養)조건에서, 근부(根部)는 무영양(無營養) 조건에서 생육 억제율(抑制率)이 큰 경향이었다.

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Effect of the Yea-Sacc yeast culture on growth performance, nutrient digestibility and fecal score in weanling pigs

  • Li, Yanjiao;Li, Tianshui;Kim, Inho
    • 농업과학연구
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    • 제46권2호
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    • pp.229-237
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    • 2019
  • The influence of dietary supplementation with a yeast culture on growth performance, fecal score and nutrient digestibility was evaluated in weaned pigs in a 6-week feeding trial. A total of 50 weaning pigs with an initial average body weight (BW) of $7.46{\pm}1.60kg$ were randomly allotted into 1 of 2 dietary treatments according to the initial BW. There were 5 replicate pens in each treatment with 5 pigs per pen. The dietary treatments were as follows: 1) control, basal diet (CON) and 2) 0.10% yeast culture, basal diet supplemented with 0.1% yeast culture (YC). The average daily feed intake was significantly improved with the dietary supplementation of the yeast culture compared with the control during phases 1 and 3. Overall (0 to 6 weeks), dietary supplementation with the 0.1% yeast culture had a significant effect on the feed conversion ratio (FCR). There was no significant difference in the fecal score between the CON and YC dietary treatments. In addition, no difference in the apparent total tract digestibility was observed between the CON and YC dietary treatments. Collectively, the results of this study indicate that dietary supplementation of 0.1% yeast only improved the feed intake of weaning pigs; however, yeast culture supplementation did not affect the average daily gain, feed efficiency, total tract digestibility of dry matter, and nitrogen and energy levels as well as the fecal scores.

한국인삼 양액재배시 배지의 영향 (Effect of Substrate on the Production of Korean Ginseng(Panax ginseng C.A. Meyer) in Nutrient Culture)

  • Dong Sik Yang;Gung Pyo Lee;Park, Kuen Woo
    • 생물환경조절학회지
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    • 제11권4호
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    • pp.199-204
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    • 2002
  • 한국의 인삼재배시 연작장해로 인한 생산량 감소가 문제가 되고 있다 이를 극복하기 위하여 본 연구에서는 한국인삼 재배를 위하여 양액재배 시스템을 적용하였다. 최적의 양액재배용 배지를 선발하기 위하여 모래, TSK-2 (S+T), 피트모스 (P), 재활용 암면 (RR)과 입상 암면 (GR)등의 다양한 배지를 단독 및 혼합처리구로 사용하였다. RR배지의 경우 전반적인 물리화학적 특성은 기존에 보고된 인삼배지의 토양특성과 유사하였다. S+T배지는 가밀도가 인삼용 토양보다 높았다 인삼의 지상부 생체중은 RR배지와 S+T배지에서 높았다. 인삼 뿌리의 생체중 및 건물중은 RR배지에서 가장 높았다. 인삼의 뿌리의 품질적 측면에서 vitamin C함량은 양액재배로 생육시킨 인삼이 토양에서 생육한 인삼보다 높았으나, crude saponin과 ginsenosides 함량에서는 차이를 나타내지 않았다.

순환식 상추 양액재배시 양액재활용 기술 (Reusing Techniques of Nutrient Solution for Recycling Hydroponic Culture of Lettuce)

  • 이성재;서명훈;이상우;심상연;이수연
    • 생물환경조절학회지
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    • 제8권3호
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    • pp.172-182
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    • 1999
  • 수경재배에서 양액의 재활용이 상추의 생육이나 수량에 어떠한 영향을 미치는지 알아보고자 Control, 양액보충, EC조절, 양액검정보정, 양액검정보정+humus첨가 등의 5처리를 두어 실험을 수행한 결과는 다음과 같다. 양액의 pH에서 양액검정보정구와 양액 검정보정.+Humus참가구가 4차재배까지 7.0이하로 안정된 경향을 나타내었고, EC는 양액보충구가 재배횟수가 늘어날수록 현저히 떨어졌고 다른 처리는 대조구와 유사한 경향이었다. 생육 및 수량은 양액검정보정 처리가 양액내 무기이온들의 균형으로 대조구와 유사한 경향을 나타내었으며 양액보충구 및 EC조절구는 재배횟수가 늘어날수록 떨어지는 경향이었다. 양액의 무기성분중 N $O_3$-N와 N $H_4$-N는 재배후 처리에 관계없이 농도가 크게 떨어졌고 특히 Ca과 Mg은 모든 처리구에서 재배횟수에 관계없이 재배전에 비해 재배 후에 양액내에 축적되는 경향을 보였다. 상추엽중 무기성분함량은 T-N와 P$_2$ $O_{5}$ 의 경우 1차재배후 양액검정보정+Humus 첨가구에서 다른 처리에 비해 함량이 적었고 재배횟수가 늘어날수록 처리간에 차이가 없었다. K과 Mg은 재배횟수나 처리간에 차이가 없었고, Ca의 경우 양액검 정보정+Humus 첨가구에서 1차와 2차재배 후 다소 많았으나, 재배횟수가 늘어날수록 처리간에 차이가 없었다.

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Pleurotus ostreatus 영양원의 수입국별 재배 가능성 검토 (Possibility of Cultivation of Pleurotus ostreatus Nutrition by Importing Country)

  • 조용구;박윤진;오태석;김창호;오민교;이영수;장명준
    • 한국유기농업학회지
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    • 제25권4호
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    • pp.749-758
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    • 2017
  • This Research was the standardization of nutrient sources by reviewing the possibility of cultivation and analysis of imported nutrient sources used in Pleurotus ostreatus culture. As a first result, the concentration of heavy metals was not detected or trace, and the total nitrogen content of cotton seed pomace was 6.22% in Australia, which was higher than that of Chinese or USA. As a second result of physical properties of nutrient sources, the water holding capacity of beet pulp from Egypt was higher with increasing pressure. The volume water content of US was highest at 400hpa and 500hpa in case of cottonseed meal. Third, the content of total nitrogen was the highest at pH 5.51 and the total nitrogen content was the highest at 2.45, and the C/N ratio was the highest at 28.18. Last result shows that first germination day, yield and biological effciency of all treatments were not significant differnet except T2. These results shows that australian cootton was inappropriate to Pleurotus ostreatus culture. Nevertheless, the safety and standardization of the Pleurotus ostreatus culture medium should be assured by reviewing the media low material importing season, mushroom yield and nutrient sources.

High Cell Density Culture of Anabaena variabilis with Controlled Light Intensity and Nutrient Supply

  • Yoon, Jong-Hyun;Shin, Jong-Hwan;Ahn, Eun-Kyung;Park, Tai-Hyun
    • Journal of Microbiology and Biotechnology
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    • 제18권5호
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    • pp.918-925
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    • 2008
  • Controlling the light energy and major nutrients is important for high cell density culture of cyanobacterial cells. The growth phase of Anabaena variabilis can be divided into an exponential growth phase and a deceleration phase. In this study, the cell growth in the deceleration phase showed a linear growth pattern. Both the period of the exponential growth phase and the average cell growth rate in the deceleration phase increased by controlling the light intensity. To control the light intensity, the specific irradiation rate was maintained above $10\;{\mu}mol/s/g$ dry cell by increasing the incident light intensity stepwise. The final cell density increased by controlling the nutrient supply. For the control of the nutrient supply, nitrate, phosphate, and sulfate were intermittently added based on the growth yield, along with the combined control of light intensity and nutrient concentration. Under these control conditions, both final cell concentration and cell productivity increased, to 8.2 g/l and 1.9 g/l/day, respectively.

실파의 수경재배에 적합한 양액종류 선발 (Selection of Proper Nutrient Solution Existing for the Water Culture of Young Welsh Onion)

  • 원재희;전신재;김상수;박권우
    • 생물환경조절학회지
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    • 제14권4호
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    • pp.263-268
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    • 2005
  • 실과용으로 적합한 양액종류를 선발하고자 수경재배용으로 선발한 '금장외대파'와 '토쿄구로파'을 공시하여 담액식으로 시험을 수행하였다. 양액종류로 기존 파용 지바농시액, 엽채소용 M식액, 파용 타케가와액, 및 싹파용 야마자키액을 이용하여 시험한 결과, 구당 생체 중 및 건물중에 있어서 두 품종 모두 야마자키액, M식액, 지바농시액, 타케가와액 순으로 양호하였다. 생체중과 건물중 증가, 그리고 양분 흡수율 및 기타 요인을 모두 고려하였을 때 실파 수경재배용으로 가장 적정한 배양액으로 싹파용 야마자키액을 선발하였다.

한국인 다빈도 섭취 과일의 영양관련 지수 분석 (Analysis of Nutritional Index of Frequently Consumed Fruits in South Korea)

  • 유지현;임정은;임현정;조여원
    • 한국식생활문화학회지
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    • 제29권6호
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    • pp.659-667
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    • 2014
  • Fruits are good sources of vitamins, minerals, fiber, and phytochemicals, which are known to reduce serum lipids, oxidative damage, and blood pressure as well as improve blood glucose control. The purpose of this study was to estimate nutrient quality indices of fruits by carrying out a critical analysis of pre-existing methods according to their nutritional compositions. Four methods were used to assess the nutrient indices of 26 fruits, which are frequently consumed by Koreans based on the fourth Korean National Health and Nutrition Examination Survey (2009). Naturally nutrient rich score (NNR), nutrient rich food (NRF), nutrient adequacy score (NAS), and nutrient density score (NDS) were used to calculate nutrient quality indices. The Korean Nutrition Society Food Composition database of fruits based on 100 g edible portions was used. The algorithm of each method included the mean percentage of daily values (Dietary Reference Intakes for Koreans, 2010) for particular nutrients based on consumption of 1,900 kcal/day. The relative score indicated that strawberries, kumquat, and lemon had high nutrient quality indices. In addition, mango, lemon, persimmon, strawberry, apricot, and tangerine fruits are rich in antioxidant nutrients such as ${\beta}$-carotene, vitamin C, vitamin E, and selenium. However, scores of nutrient quality indices did not imply that higher scores of particular fruits are superior. We suggest moderate seasonable consumption a variety of fruits. Our results can be used as a reference for consumers when they choose fruits.