• Title/Summary/Keyword: number of stomata

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Photosynthesis, Antioxidant Enzyme, and Anatomical Difference of Sedum kamtschaticum and Hosta longipes to Ozone (오존이 기린초와 비비추의 광합성, 항산화효소, 해부학적 구조에 미치는 영향)

  • Cheng, Hyo-Cheng;Woo, Su-Young;Lee, Seong-Han;Baek, Saeng-Geul
    • Horticultural Science & Technology
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    • v.28 no.3
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    • pp.394-402
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    • 2010
  • The objective of this study was to identify the effects of ozone on the two species in controlled environment. $Sedum$ $kamtschaticum$ and $Hosta$ $longipes$ were exposed in both control and ozone chamber to investigate photosynthesis, antioxidant enzyme activity, visible damage, the number and the size of stomata and the plastogloubuli. Ozone was fumigated in the concentration of $200{\mu}g{\cdot}kg^{-1}$ for 8 hours in a day (from 08:00 AM to 04:00 PM). Firstly, net photosynthesis of two species was decreased after ozone fumigation. Secondly, glutathione reductase activities showed significant difference between control and ozone treatment. Thirdly, visible symptoms of leaves were expressed such as chlorosis, necrosis and decoloration. Also, the size of stoma was significantly decreased in ozone-exposed plants. Furthermore, the intercellular space of $Hosta$ $longipes$ showed increased phenomenon because the mesophyll was collapsed. The number and the size of the plastogloubuli were significantly larger in ozone stress.

Variation of Genus Ilex in Korea and their Ornamental Values (Ilex속(屬) 수목(樹木)의 유전변이(遺傳變異)의 분석(分析)과 조경학적(造景學的) 이용가치(利用價値)의 조사(調査) 연구(硏究))

  • Yim, Kyong Bin
    • Journal of Korean Society of Forest Science
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    • v.42 no.1
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    • pp.1-38
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    • 1979
  • The woody species of Genus Ilex which are endemic to Korea are distributed on limited area due to solely temperature factor. There is some differences according to species, however in general, the evergreen Ilex are found along southern coastal area of Korean Peninsula and near islands where the cold index does not exceed $-5^{\circ}C$. But Ilex macropoda and the variety, only deciduous ones, are grown in temperate zone of the peninsula and some islands. The list of Ilex species of Korea are as follows. Ilex cornuta Lindley et Pax., I. crenata Thunb. var. microphylla Max., I. crenata Thunb., I. rotunda Thunb., I. macropoda Miq., I. macropoda Miq. var. pseudo-macropoda Loensner, I. integra Thunb. The author surveyed the populations of Ilex species as many as possible and data of some characters such as leaf shape, spine, fruit shape, stomata density, sex ratio in natural communities, etc. are collected. Almost all the Ilex species in Korea show sporadic distribution. This means quite small sized populations isolate distantly each other eliminating the change of gene exchange in between. Particularly Ilex conuta and I. crenata show the morphological differentiation among populations as well as significant individual variation within a population. These were true with such characteristics, leaf shape, leaf dimension, leaf margin, fruit shape, spine, and stomata density. The founded are that the fruit length and the stomata density counted on the beneath surface of leaves of Ilex cornuta increased with the decrease of latitude. These are naturally closely related with the cold index values. The table shown below indicates the correlation between mean stomata density per $0.3642mm^2$ and cold index values. These relation however were not observed on Ilex crenata. The most dominated natured in relation to individual variation were outline of leaf, the number of marginal spine, the shape of leaf cross section and the degree of luster of the upper leaf surface. As shown in photos 5~7, these variations are agreed at a glance. There are reports that the development of marginal spines in some Ilex species is associated with the juvenility and topophysis. In present study, these two factors were neglected because of the intended sampling procedure. Of Ilex rotunda, population difference with the characteristics of leaf length is recognized but not for leaf width, petiole length, and fruit size. However, individual variations within a population were significantly large. In case of Ilex integra, only individual differences within population were calculated statistically for such characteristics as leaf length, leaf width, and petiole length. As to natural population, the sex ratio was 1:2 (female to male) for Ilex cornuta, and 1:1 for Ilex crenata. The tendency of more male than female in I. cornuta was agreed to other observations. Preparing the tip cutting of length 10cm, and treating with IBA, then attaching earth ball to the cut end, very successful rooting percentages were obtained. Asexual propagation has the advantages of maintaining the heterozygosity of existing varieties and overcoming the difficulties of delayed seed germination frequently encountered with Ilex species. Considering a great deal of variation in morphological traits, a good possibility of selection breeding for decorative and ornamental purposes exists. At present, these evergreen Ilex are ignored by local people as nuisance weedy shrubs. So the proper protection measures should promptly be taken.

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Radiation Effect of X-Ray and Thermal Neutron on Robinia pseudoacacia L. and Some Other Species (아까시나무외 몇 수종(樹種)에 대(對)한 X-Ray와 Thermal Neutron의 조사효과(照射効果))

  • Kim, Chung Suk;Lee, Suk Koo;Hyun, Sin Kyu
    • Journal of Korean Society of Forest Science
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    • v.17 no.1
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    • pp.1-15
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    • 1973
  • In an effort to improve the major tree species in Korea, the seed of Robinia pseudoacacia, Pinus rigida, Pinus densiflora, Pinus thunbergii and Larix leptolepis were treated with X-ray and thermal neutron at the Brookhaven National Laboratory, and germination rate of the seed and some characteristics of the seedlings from irradiated seed were investigated and the results were summarized as follows. 1. The germination rate of the irradiated seed of Robinia pseudoacacia, Pinus densiflora, Pinus thunbergii and Pinus rigida was decreased, when the irradiation time of thermal neutron increased from 3 hours to 9 hours. The seed of Larix leptolepis was completely died out in all range of irradiation time. 2. The seed of Pinus densiflora, Robinia pseudoacacia and Pinus rigida showed low germination rate, when the dosage of radiation increased in the range of 10,000r-30,000r X-ray. This dosage of radiation was almost lethal to the seed of Pinus thunbergii and Larix leptolepis. 3. The growth rate of radiated Robinia pseudoacacia has been decreased when the dosage of X-ray and thermal neutron increased. However, the trees treated with thermal neutron for 3 hours showed 14.9 percent-increase in seedling height and some thornless individuals appeared in this treatment. 4. Individuals with variegated leaf, rugose leaf and albino were appeared in X-ray and thermal neutron treatment. 5. Abnormal mitosis of somatic cell, cell with two nucleoli, cell with two nuclei and chromosome clump in mitosis of somatic cell were observed in Robinia pseudoacacia irradiated with thermal neutron. 6. Resistanty against pawdery mildew was decreased in Robinia pseudoacacia radiated with X-ray and thermal neutron. 7. Length of stomata did not show any difference however number of stomata per unit area decreased in Robinia pseudoacacia radiated with thermal neutron. The leaves of Robinia pseudoacacia radiated with thermal neutron were thicker than those of non-treated one, but width of palisade tissue was decreased. The most sensitive one among those species to the thermal neutron treatment was Larix leptolepis, followed by Pinus densiflora, Robinia pseudoacacia, Pinus thunbergii and Pinus rigida in the order. In X-ray treatment, the most sensitive one was Larix leptolepis, followed by Pinus densiflora, Pinus thunbergii, Pinus rigida and Robinia pseudoacacia in the order. Morphological, cytological variation of the radiated Robinia pseudoacacia seemed to indicate some possibility to be used for tree improvement.

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Effects of Depth and Duration of Water-logging on Growth and Yield at Transplanting and Flowering Stage in Pepper (Capsicum annuum L.) (고추(Capsicum annuum L.)의 이식기(移植期) 및 개화기(開化期) 침수처리(浸水處理) 따른 생육반응(生育反應))

  • Guh, Ja-Ock;Kuk, Yong-In
    • Korean Journal of Environmental Agriculture
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    • v.15 no.4
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    • pp.425-433
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    • 1996
  • Pepper plants were water-logged at 0, 5, 10 and 15 cm at transplanting and flowering stages under the condition of greenhouse. Treatment of water-logging times were 6, 12, 24, 48 and 120 hours. The results obtained are summarized as follows. At the transplanting stage, plant height, number of leaves, shoot and root fresh weight decreased by water-logging at 0cm for 24 hours and at 5cm or more for 6 hours. Number of fallen leaf was negligible by 12 hours water-logging at 0cm, however, its increased by more increased the water-logging depth and time. Diffusion resistance and chlorophyll content of leaf, and root activity decreased at more than 24 hours of water-logging regardless of the water-logging depth treatments. Photosynthesis and respiration rate diminished by increased the water-logging depth at 120 hours water-logging treatment. Plant diseases, mainly anthracnose(Colletotrichum) occurred in proportion to increase the depth and time of water-logging. It was not possible to control the diseases by fungicides. At the treatment of foliar spray of urea for recovery to water-logging damage, the efficiency was not found on plant height, but the number of leaves. Number of fruit and weight of fruit per plant showed no difference from no water-logging to 24 hours water-logging at 0cm, but its decreased that more than 24 hours water-logging at 0cm and more than 6 hours water-logging at 5cm or more. The averaged weight of a fruit on survival plants increased by more hours and deeper water-logging. There was positive correlation between all the investigated characteristics of growth and yield. There was, however, negative correlation between the characteristics and diffusion resistance of leaf stomata. The correlation between number of fallen leaf and averaged weight of a fruit was not significant. At flowering stage, number of fruit and weight of fruit per plant showed a similar tendency to no water-logging and by 12 hours water-logging at 0cm and 5cm, but significantly decreased at more than 24 hours water-logging from 0 to 5cm, and more than 6 hours water-logging at 10cm or more. The averaged weight of a fruit on survival plants increased by more hours and deeper water-logging except for 120 hours water-logging at all water depths.

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Studies on the changes in phenological, growth and physiological responses of Silene capitata Kom., an endangered plant in Korea, under climate change treatment

  • Park, Jae-Hoon;Han, Young-Sub;Lee, Eung-Pil;Lee, Seung-Yeon;Jeong, Heon-Mo;You, Young-Han
    • Journal of Ecology and Environment
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    • v.43 no.3
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    • pp.305-313
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    • 2019
  • Background: This research aims to study the effect of climate change on the phenology, growth, and physiological traits of Silene capitata Kom., a Korean endangered species II. This study increased $CO_2$ concentration in a closed glass greenhouse, with the daily mean temperature and $CO_2$ concentration respectively being $4.61^{\circ}C$ and 93.63 ppm higher than the outside temperature (ambient conditions, control). The seeds of S. capitata were sown in control and treatment environments in March 2013 while seedlings were transplanted into individual pots in May 2013. To research phenological changes, the first day of the flowering and ripening of the plants transplanted in 2013 and first day of leafing in 2014 were observed. The growth and physiological responses of mature leaves were also studied in 2013. Results: There was no difference in the first day of flowering, but the first day of ripening was earlier in the treatment group than the control group. There was no difference in the number of rosette leaves between the two groups, but leaf area was wider in the treatment group than the control group. Transpiration rate and stomatal conductance were higher in the treatment group than the control group, chlorophyll content decreased, and photosynthetic rate and water use efficiency were the same for both groups. As a result of simple regression analysis among the transpiration rate, stomatal conductance, photosynthetic rate, and water use efficiency, stomatal conductance increased when transpiration rate increased. Stomatal conductance increased with photosynthetic rate in the control unlike in the treatment group. The photosynthetic rate and water use efficiency increased with transpiration rate in the control group unlike in the treatment group. Furthermore, water use efficiency increased as photosynthetic rate increased in both groups. Conclusion: Due to high $CO_2$ concentration, the photosynthetic rate was no longer controlled by the stomata, which appeared to suppress the excessive production of photosynthetic products by reducing chlorophyll content. It is believed that the phenological responses of S. capitata under climate change conditions will advance and that stable growth will be difficult in regions lacking moisture due to the high transpiration rate.

Pink Pigmented Facultative Methylotrophic Bacteria(PPFMs): Introduction to Current Concepts (분홍색 색소를 형성하는 methylotrophic acteria(PPFMs): 최근 경향소개)

  • Munusamy, Madhaiyan;Sa, Tongmin;Kim, Jai-Joung
    • Korean Journal of Soil Science and Fertilizer
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    • v.37 no.4
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    • pp.266-287
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    • 2004
  • The non infecting, plant associated bacteria have attracted increased attention for stimulating plant growth and as environmental friendly plant protecting agents. Pink-pigmented facultatively methylotrophic bacteria (PPFMs), classified as Methylobacterium spp., are persistent colonizers of plant leaf surfaces. As the leaves of most or all plants harbor PPFMs that utilize leaf methanol as their sole source of carbon and energy, which is a specific attribute of the genus Methylobacterium. Although they are not well known, these bacteria are co-evolved, interacting partners in plant metabolism. This claim is supported, for example, by the following observations: (1) PPFMs are seed-transmitted, (2) PPFMs are frequently found in putatively axenic cell cultures, (3) Low numbers of seed-borne PPFMs correlate with low germinability, (4) Plants with reduced numbers of PPFM show elevated shoot/root ratios, (5) Foliar application of PPFMs to soybean during pod fill enhances seed set and yield, (6) Liverwort tissue in culture requires PPFM-produced vitamin B12 for growth, (7) treated plants to suppress or decrease disease incidence of sheath blight caused by Rhizoctonia solani in rice, and (8) the PPFM inoculation induced number of stomata, chlorophyll concentration and malic acid content, they led to increased photosynthetic activity. Methylobacterium spp. are bacterial symbionts of plants, shown previously to participate in plant metabolism by consuming plant waste products and producing metabolites useful to the plant. There are reports that inform about the beneficial interactions between this group of bacteria and plants. Screening of such kind of bacteria having immense plant growth promoting activities like nitrogen fixation, phytohormone production, alleviating water stress to the plants can be successfully isolated and characterized and integration of such kind of organism in crop production will lead to increased productivity.

The Variation of Needle Characteristics of Picea jezoensis(Siebold & Zucc.) Carriere Populations in Korea (한국산 가문비나무 자생집단의 침엽특성 변이)

  • Song, Jeong-Ho;Hur, Seong-Doo;Kang, Kyu-Suk;Yang, Byeung-Hoon;Lee, Jung-Joo
    • Korean Journal of Plant Resources
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    • v.22 no.1
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    • pp.31-36
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    • 2009
  • The aim of this study was to examine geographic variation to support a genetic resource conservation plan of rare endemic Picea jezoensis (Siebold & Zucc.) Carriere. Three populations of P. jezoensis were analysed using multivariate analysis for 7 characteristics of needle morphology. In the morphological characters, seven characters of needle were 15.11 mm (needle length), 1.43 mm (needle width), 0.42 mm (needle thickness), 10.9 (length / width of needle), 3.6 (width / thickness of needle), 0.47 mm (Distance between resin duct and vascular), 17.4 ea. (number of stomata row), respectively. Nested analysis showed that were statistically significant differences among populations as well as among individuals within populations in 6 quantitative characters. In 5 of 7 characters, variance components among populations were higher than those among individuals within populations. Cluster analysis using complete linkage method showed two groups (Mt. Gyebang populations was genetically distinct from the other two populations) to Euclidean distance 0.4. Among principal components, primary 2 principal components appeared to be major variables because of the loading contribution of 100%.

Taxonomic Examination of Isoëtes L. (Isoëtaceae) in South Korea (우리나라 물부추속 (물부추과)에 대한 분류학적 고찰)

  • Jung, Jongduk;Kim, Changkyun;Kim, Hojun;Choi, Hong-Keun
    • Korean Journal of Plant Taxonomy
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    • v.39 no.2
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    • pp.63-73
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    • 2009
  • $Iso{\ddot{e}}tes$ L. ($Iso{\ddot{e}}taceae$) is difficult to identify among the infrageneric taxa because of morphological simplicity. Since two species (I. japonica and I. coreana) had been reported, I. jejuensis and I. hallasanensis were described as two new species from Jeju Island, South Korea. The habitats of all species were confirmed by current studies except for I. japonica. We compared the morphological and anatomical characters of seven species, three domestic species and four species in adjacent regions (I. taiwanensis, I. sinensis, I. japonica, and I. asiatica). I. asiatica differs from other species in the lobe number of corm, spreading sporophylls, lack of stomata and degree of velum development. All of the seven species were identified by the characteristics of their mega- and microspores. Especially, it was possible to identify the four species in South Korea by the types of ornamentation on their megaspores. Each species has a specific type of megaspore ornamentation: I. coreana - cristate, I. jejuensis - rugulate, I. hallasanensis - echinate, and I. japonica - reticulate. We also discussed the phylogeny of the genus in East Asia in relation to recent studies based on the second intron of LEAFY sequence and amplified fragment length polymorphism (AFLP) analysis. We suggest strategies for the conservation of the endangered $Iso{\ddot{e}}tes$ species in South Korea.

The Variation of Isoenzymes and Morphological Characteristics of Needle, Cone and Seed According to Resin Duct Index in Pinus densiflora and Pinus thunbergii (소나무 및 곰솔의 수지구지수(樹脂溝脂數)에 따른 침엽(針葉), 구과(毬果) 및 종자(種子)의 형태적(形態的) 특성(特性)과 동위효소(同位酵素)의 변이(變異))

  • Son, Doo Sik;Park, Sang Jun;Hwang, Jae Woo
    • Journal of Korean Society of Forest Science
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    • v.79 no.4
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    • pp.424-430
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    • 1990
  • The variation of needle, cone and seed characteristics and of allelic frequency in isoenzyme, ADH, ME and PGI, according to different resin duct index in Pinus densiflora, Pinus thunbergii and their hybrids was analyzed. The results obtained were as follows : 1. With increase of number of resin ducts, morphological characteristics such as needle length, needle sheath length, cone size, seed size, seed wing size, 1000 seeds weight, etc. tended to he increased, while number of stomata row in needle to be decreased. 2. As the results of discriminant analysis for the morphological characteristics of needle, cone and seed, most individuals are generally coincided with number of resin duct mostly in Pinus densiflora were not. 3. According to the canonical discriminant function obtained from the morphological characteristics in Pinus densiflora, Pinus thunbergii and their hybrids including introgressive hybrid, the resin duct index, 1000 seeds weight, cone size and neelde sheath length characterized fairly their species. 4. With increase of resin duct index, hybrid index tended to be higher. The results obtained from the discriminant analysis and the hybrid index were nearly same each other. 5. With increase of number of resin duct, the allelic frequencies for isoenzyme, ADH-$B_2$, ME-$A_2$ and PGI-$B_1$. $B_2$ tended to increase but those of ADH-$B_3$, ME-$A_4$, and PGI-$B_3$, to decrease. It is thought that this increase of frequency for the former four isoenzymes was resulted in introgressive gene flow from Pinus thunbergii to Pines densiflora and accordingly the frequency of latter three isoenzymes tended to decrease.

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Effects of Uniconazole Treatment on the Growth and Floweringof Potted Chrysanthemum indicum L. (Uniconazole 농도가 분화용 감국의 생육 및 개화에 미치는 영향)

  • Jung, Sung Sook;Jeong, Hyun Hwan;Kim, Ki Sun
    • Horticultural Science & Technology
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    • v.18 no.1
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    • pp.28-32
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    • 2000
  • This study was carried out to investigate the effects of uniconazole treatment on the growth and flowering of potted Chrysanthemum indicum L. for high quality pot plant production. Uniconazole was drenched at 0.05, 0.01, or 0.15 mg a.i./pot at 14 days after planting (DAP) of rooted cuttings. Simultaneously the short-day treatment (SDT) and pinching were adapted. The same amount of uniconazole (0.05 mg a.i./pot) was spilt drenched at once, twice, and three times, respectively, at 1 week interval. Uniconazole markedly reduced plant height, branch length, and stem diameter. Plant height was reduced linearly with increasing uniconazole concentration at 0.05, 0.01, or 0.15 mg a.i./pot up-to 41.6%, 52.5%, and 58.5%, respectively. In 0.05 mg a.i./pot, the number of branches greatly increased and plant height of 22.6 cm was adequate for pot plant. However, higher concentrations (0.10, 0.15 mg a.i.) were not suitable for production of high quality pot plant (17.0, 14.8 cm, respectively). Pinching and SDT decreased the number of days to visible bud, while uniconazole treatments delayed days to visible bud by 5-9 days compared with pinching and SDT. Number of visible buds was highest at 0.05 mg a.i./pot uniconazole treatment. However, flower diameter was decreased by uniconazole treatment, resulting in compact form. Number of stomata was increased by uniconazole treatment. The length of vascular tissues of uniconazole-treated plants ($11.2{\mu}m$) was smaller than that of non-treated plants ($15.0{\mu}m$, and the size of xylem vessel was also decreased. Uniconazole treatment at 0.05 mg a.i./pot at 14 DAP with pinching and SDT were recommended for pot plant production of C. indicum L.

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