• 한국미생물·생명공학회지
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• 제20권1호
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• pp.40-45
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• 1992

Aspergillus niger로부터 acidic nucleotidase를 Sepharose CL-6B gel 여과와 DEAE-Sephacel 이온교환수지를 이용하여 부분정제하였다. 5'-AMP 와 3'-AMP를 기질로 사용했을 경우에 효소의 최적 pH는 4.5, 그리고 최적온도는 $55^{\circ}C$였다. 그러나, p-nitrophenyl phosphate를 기질로 사용했을 경우에는 최적 pH는 변화가 없었으나, 최적 온도는 $70^{\circ}C$였다. 효소의 활성화에너지는 3'-AMP, 5'-AMP 그리고 p-nitrophenyl phosphate를 기질로 사용했을 경우에 각각 4.76kcal/mole, 6.95kcal/mole 그리고 11.82kcal/mole 였다.

• 생약학회지
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• 제20권3호
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• pp.154-161
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• 1989

Effects of panaxydol, panaxynol and panaxytriol isolated from Panax ginseng C.A. Meyer on some enzyme activities were determined. Activities of ATPase, membrane-bound enzyme from Sarcoma 180 and rat liver were slightly inhibited by panaxydol. Activities of 5'-nucleotidase, membrane-bound enzyme and succinate cytochrome c reductase in mitochonidria from sarcoma 180 and rat livers were significantly inhibited in a dose-dependent manner by panaxynol. The inhibitory effects of panaxydol and panaxynol on succinate cytochrome c reductase activities were more potent than those on 5'-nucleotidase activities and panaxynol was found to be a very potent inhibitor of succinate cytochrome c reductase. Activities of glucose-6-phosphatase in endoplasmic reticulum from Sarcoma 180 and rat livers were not affected by all three polyacetylenes. These results suggested that the inhibitory effects of panaxydol and panaxynol on enzyme activities might contribute to their biological activities.

• Asian Pacific Journal of Cancer Prevention
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• 제16권3호
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• pp.959-963
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• 2015

Purpose: To find parameters that can increase alpha-fetoprotein (AFP) sensitivity and so help in accurate diagnosis and rapid management of hepatocullular carcinoma (HCC), as AFP has limited utility of distinguishing HCC from benign hepatic disorders for its high false-positive and false negative rates. Materials and Methods: Serum levels of AFP, 5'-nucleotidase enzyme activity (5-NU) and leucine aminopeptidase enzyme (LAP) activity were measured in 40 individuals. Results: LAP and 5'NU were elevated in HCC at p<0.001. Pearson correlation coefficients showed that changes in AFP exhibited positive correlation with both 5'-NU and LAP at (p<0.001). The complementary use of LAP only with AFP resulted in an increase in sensitivity of AFP from 75% to 90% in detecting HCC. The complementary use of both LAP and 5-NU with AFP resulted in an increased sensitivity of AFP in detecting HCC from 75% to 95%. Conclusions: LAP and 5-FU can be determined in HCC patients in combination with AFP to improve its sensitivity and decrease false negative results.

• Journal of Applied Biological Chemistry
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• 제42권4호
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• pp.169-174
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• 1999

Arabidopsis AHL gene contains 4 exons encoding a putative protein highly homologous to the yeast salt-sensitive enzyme HAL2, a 3'(2'),5'-bisphosphate nucleotidase involving in reductive sulfate assimilation. AHL cDNA complemented yeast met22 (hal2) mutant. AHL fusion protein expressed in E. coli exhibited $Mg^{2+}$-dependent, 3'-phosphoadenosine 5'-phosphate (PAP)-specific phosphatase activity. $Li^+,\;Na^+,\;K^+$ and $Ca^{2+}$ ions inhibit the enzyme activity by competing with $Mg^{2+}$ for the active site of the enzyme. The enzyme activity was also sensitive to ${\mu}M$ concentrations of toxic heavy metal ions such as $Cd^{2+},\;Cu^{2+}$ and $Zn^{2+}$, but was not recovered by addition of more $Mg^{2+}$ ions, suggesting that these ions inactivate the enzyme with a mechanism other than competition with $Mg^{2+}$ ions. Inhibition of the AHL enzyme activity may result in accumulation of PAP, which is highly toxic to the cell. Thus, the AHL enzyme could be one of the intial targets of heavy metal toxicity in plants.

• 대한약학회:학술대회논문집
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• 대한약학회 2000년도 NEW STRATEGY FOR DRUG DEVELOPMENT IN POST-GENOMIC ERA(대한약학회)
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• pp.186.2-187
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• 2000

• 한국식품과학회지
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• 제21권1호
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• pp.1-8
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• 1989

네 지역에서 2년 동안 재배된 10종의 맥주맥을 제맥하여 맥아중의 핵산분해에 관련된 6종류의 효소를 측정하였다. 측정된 효소는 데옥시리보핵산 가수분해효소, 리보핵산 가수분해효소 포드포디에스테르 가수분해효소, 3'-뉴클레오티드 가수분해효소, 5'-뉴클레오리드 가수분해효소와 포스포모노 에스테르 가수분해효소 이었다. 5일 동안 발아시킨 맥아속의 효소는 맥주맥의 품종이나 재배지역에 따라 크게 영향을 받았고. 현재 장려품종으로 재배되고 있는 몇몇 품종은 이러한 효소들을 상당량 함유하고 있었다. 80시료의 리보핵산 가수분해효소, 데옥시리보핵산 가수분해효소, 3‘-뉴클fp오리드 가수분해효소와 5'-뉴클레오리드 가수분해효소의 평균 함량은 각각 11.2, 5.7, 5.6과 1.2 units이었다. 6조 맥의 맥아는 2조 맥의 맥아보다 데옥시리보핵산 가수분해효소, 포스포디에스테르 가수분해효소와 3'-뉴클레오리드 가수분해효소를 더 많이 함유하고 있었고, 2조 맥의 맥아는 리보핵산 가수분해효소 합량에서 6조 맥의 맥아보다 더 높았다.

• 한국식품영양과학회지
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• 제14권2호
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• pp.137-144
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• 1985

지의류(地衣類)의 수용성 추출물이 간손상에 미치는 효과를 조사하고자 지의류 추출물을 투여한 실험군과 물을 준 대조군으로 나누어 사육하면서 흰쥐 체내의 체중 및 각 장기무게, GOT, GPT 및 5'-nucleotidase의 활성을 측정하였다. 실험은 3차에 걸쳐 행하였는데 제1차 실험은 정상 쥐에게 지의류(地衣類)를 투여하였고, 제2차 실험은 지의류(地衣類) 투여에 따라 무처리(無處理), 전처리(前處理), 후처리(後處理)군으로 나눈 후 흰쥐들에게 $CCl_4$를 주사하여 1주일동안 변화를 측정하였으며, 제3차 실험은 $CCl_4$에 의한 급성 및 만성 간손상을 가진 쥐들에게서 지의류(地衣類) 추출물의 효과를 관찰한 결과를 요약하면 다음과 같다. 정상 쥐의 혈청내 GOT 및 GPT 활성은 실험군이 대조군보다 비교적 낮았으며 간손상 유도시 지의류(地衣類) 전처리(前處理)군 및 후처리(後處理)군에서 혈청내 효소활성 상승 억제효과를 볼 수 있었다. 급성 간손상시 실험군의 혈청내 효소활성도가 낮았으나 간장조직내의 GOT 활성은 실험군이 대체로 높았으며 GPT 활성은 두군간에 별 차이가 없었다. 5'-nucleotidase는 GOT와 같은 경향이었다. 혈청내 효소량을 낮게 유지하는데 만성장애시 보다 급성장애시 더 효과적이었으며 간장조직내 효소의 상대적 변화는 혈청에 비해 적었다. 각 장기무게는 정상쥐에서는 특이한 점이 없었으나 $CCl_4$ 주사 후 지의류 추출물을 투여했을 때 대조군에 비해 실험군의 간장무게가 증가하였다. 간장 및 비장의 핵산함량과 적혈구, 백혈구 및 hemoglobin 양도 실험군에서 높았다.

• 대한골관절종양학회지
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• 제7권2호
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• pp.41-50
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• 2001

목적 : 골육종 환자의 조직과 혈청에서 DNase, RNase, 5'-nucleotidase, alkaline phosphatase 및 amylase 활성도를 측정하여 그 활성도의 변동을 보았고 이들 효소가 골육종의 표지자로 이용될 수 있는지 알아보았다. 대상 및 연구방법 : 골육종 환자의 혈청과 종양조직은 1 2예로부터 얻었으며, 이에 대한 대조조직은 동일 환자의 정상부위에서 채취하였다. 조직 추출액을 얻어서 핵산, 단백함량, 그리고 효소 활성도의 측정에 사용하였다. DEAE-cellulose chromatography에 의해 골육종 조직의 acid DNase, neutral RNase, RNase inhibitor와 단백을 분리하고 그활성도와 단백 함량을 측정하였다. 결과 : 골육종 조직의 acid DNase, RNase, 5'-nucleotidase, alkaline phosphatase 활성도는 유의하게 상승하였고, 효소활성도의 양성율도 높게 나타났다. neutral RNase의 활성도가 특히 높았으며, RNase inhibitor는 neutral RNase와 복합을 이루면서 그 활성도가 유의하게 증가하였다. 혈청 neutral RNase 활성도가 유의하게 상승하였다. DEAE-cellulose column chromatography에 의해 acid DNase는 단일효소로서, 그리고 neutral RNase는 5개의 isozyme으로 분리되었다. 결론 : 이들 효소의 병용이 골육종의 생화학적 표지자로서 이용될 수 있을 가능성을 시사하였다. 또한 혈청 neutral RNase활성도의 측정이 골육종의 생화학적 표지자로서의 역할을 시사하였다. acid DNase와 neutral RNase가 골육종의 발생과 억제과정에 작용하고 있을 가능성을 시사하였다.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제12권1호
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• pp.27-40
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• 1990

In recent years, tissue antigens and enzymes that will serve as phenotypic markers for malignant cells are becoming increasingly important as diagnostic aids. This study was undertaken to investigate the specific activities of these enzymes in DMBA-induced rat submaxillary gland carcinogenesis. One hundred and twenty Sprague-Dawley rats about 100 gms of body weight were used. In experimental group, DMBA pellet (5mg) was implanted into right submaxillary gland and sham operation was performed into left gland to serve as control. The animals were sacrificed every three weeks up to 15 weeks. Submaxillary glands were excised on both sides and enzyme assays for ${\gamma}-glutamyl$ transpeptidase (GGT), 5'-Nucleotidase, Ornithine decarboxylase(ODC) and Acetyl-Co A carboxylase were carried out biochemically. The obtained results were as follows ; 1. In control group, there was no significant weight change of submaxillary gland, while experimental group, weight was increased remarkably about 7-fold at 15th week since DMBA implantation. 2. In control group, there was no change in specific activities of enzymes during the experimental period. 3. GGT activity was rapidly increased reaching a peak of 1.766${\pm}$0.082units/mg of DNA, 8-fold greater than that of onset. 4. 5'-Nucleotidase activity was increased reaching a peak of $362.1{\pm}53.2{\mu}moles/mg$ of DNA at 9th week. 5. ODC activity was rapidly increased, reaching a peak of 26.2${\pm}$4.8nmoles/mg of DNA at 9th week and quickly returned to that of control at 15th week. 6. Acetyl-Co A carboxylase activity was rapidly increased earlier than other enzymes, reaching a peak of 0.178${\pm}$0.013units/mg of DNA at 6th week and quickly declined to the control level at 15th week.

• BMB Reports
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• 제37권3호
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• pp.370-375
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• 2004

There has been increasing interest in the value of using soybean to delay or reduce the tumor incidence. This study was undertaken to investigate the possible protective effects of soybean against hepatocarcinogenesis induced by DL-ethionine. Accordingly, we measured biochemical changes occurring in serum and liver of rats treated with DL-ethionine in the presence or absence of soybean. Male albino rats were fed a control diet containing the hepatocarcinogen, DL-ethionine, or the control diet plus soybean 30%, or the control diet plus soybean plus DL-ethionine 0.25% for three months and then returned to a control diet for up to nine months. Rats fed a control diet plus DL-ethionine showed a gradual decrease in liver DNA, RNA, total protein, and liver weight and enzyme activites of liver transaminases (GOT and GPT) and alkaline phosphatase over the 7-month study period. This was followed by a large increase in the liver parameters at the end of the $9^{th}$ month, except for 5'-nucleotidase and glucose-6-phosphatase that showed a large decrease. On the other hand, a gradual increase in the serum enzyme activities of GOT, GPT, 5-nucleotidase, alkaline phosphatase, and in the albumin/globulin (A/G) ratio is observed in the group of rats fed a control diet plus DL-ethionine compared to the control group over 8 months, and this was followed by a large increase in all serum parameters studied at nine-months. The administration of 30% soybean to the rat diet in addition to DL-ethionine maintained all parameters studied at near control values until the end of the $9^{th}$ month. This study suggests that soybean has a protective effect against the hepatocarcinogenesis induced by DL-ethionine.