• 생명과학회지
• /
• 제27권11호
• /
• pp.1349-1356
• /
• 2017

$K^+$ 통로 개방제들은 심근, 뇌, 골격근 등에서 세포막 혹은 미토콘드리아 내막에 존재하는 큰 전도성의 $Ca^{2+}$-의 존성 $K^+$ (BK) 통로 및 ATP-조절성 $K^+$ 통로(ATP-sensitive $K^+$ channels, $K_{ATP}$)에 작용하여 허혈성 혹은 산화성 세포 손상을 완화하는 효과가 있는 것으로 보고되어 있다. 본 연구에서는 망막 색소 상피세포주인 ARPE-19 세포를 실험 모델로 하여 큰 전도성의 BK 통로 개방제인 NS 1619가 유사한 보호 효과를 나타낼 수 있는지, 또한 그 작용기전이 무엇인지를 확인하고자 하였다. AREE-19 세포를 여러 형태의 산화 스트레스에 노출시켜 세포 손상을 유발하고 그 손상의 정도 및 이에 미치는 NS 1619의 효과를 trypan blue 배출능, Tunel 염색 분석을 통하여 측정하였다. NS 1619는 여러 형태의 산화 스트레스에 의한 괴사성 및 apoptosis에 의한 세포 손상을 효과적으로 방지하였으며 그 보호 효과는 BK 통로 봉쇄제인 paxilline 의해 차단되었다. NS 1619는 $H_2O_2$에 의한 세포내 ATP 고갈을 현저히 완화시켰으며, 또한 MTT 환원능으로 측정한 미토콘드리아의 기능을 보호하는 효과를 보였다. 유세포형광 분석법을 이용한 실험에서 NS 1619는 $H_2O_2$에 의한 미토콘드리아 막전압의 소실을 유의하게 방지하였다. 이상의 결과들을 종합하면 NS 1619는 망막 색소 상피세포에서 산화성 세포 손상을 방지하는 효과를 나타내며 그 기전에 미토콘드리아 기능에 대한 보호 작용이 연관되어 있는 것으로 사료된다.

• 한국균학회지
• /
• 제44권3호
• /
• pp.138-144
• /
• 2016

대전광역시 연자산과 충청남도 주요 산림 토양들로부터 분리, 동정한 야생효모들 중 국내 미기록종으로 Pseudozyma prolifica HL9-1, Trichosporon coremiiforme NS19-2, Candida cretensis SA4-1, Cryptococcus diffluens TJ4-3, Cryptococcus pinus YB17-2 등의 유포자효모와 Candida vartiovaarae DD2-5, Pichia galeiformis DM3-5, Candida pseudolambica JW2-3, Trichosporon xylopini NS5-1, Trichosporon moniliiforme NS5-7, Tetrapisispora iriomotensis NS14-2, Tetrapisispora nanseiensis SA17-1 등의 무포자효모들을 선별하여 이들의 미생물학적 특성을 조사하였다. 12균주 모두 구형~타원형이었고 출아법으로 영양증식하였으며 yeast extract peptone dextrose (YPD) 배지에서 잘 생육하였다. 특히 Candida cretensis SA4-1과 Tetrapisispara iriomotensis NS14-2은 10% NaCl을 함유한 YPD 배지에서 잘 생육하는 호염성 효모들이었다. Candida vartiovaarae DD2-5외 3균주들은 유당을 자화시켰으며 Tetrapisispora nanseiensis SA17-1은 xylose를 자화시키고 동시에 발효시켰다. Candida cretensis SA4-1과 Tetrapisispara iriomotensis NS14-2 두 호염성 효모들의 생리기능성을 조사한 결과 이들의 무세포추출물들의 항고혈압성 안지오텐신 전환효소 저해활성이 각각 71.3%와 68.4%로 높았다.

• 한국정보통신학회논문지
• /
• 제14권11호
• /
• pp.2397-2402
• /
• 2010

본 논문에서는 800MHz 대역에서 다양한 시간자연 특성을 갖는 유전체 공진기 대역통과 필터를 설계 및 제작하였다. 각각의 경우에 삽입손실은 모두 2dB 이내이었고 ripple은 0.2dB 이내이었으며 반사손실은 20dB 이상의 값을 가지는 우수한 특성을 보였다. 지연시간의 경우에는 각각 6ns, 12ns, 20ns를 구현하였으며 이때 평탄도의 범위는 1ns 이내이었다. 또한 유전체 블록이 2-hole을 갖는 경우에는 2~4ns의 범위를 갖는 것을 확인하였으며 각각의 유전체 필터를 조합하여 다양한 지연시간을 갖는 유전체 공진기 필터를 구현하였다.

• 한국시뮬레이션학회:학술대회논문집
• /
• 한국시뮬레이션학회 2002년도 춘계학술대회논문집
• /
• pp.253-258
• /
• 2002

This paper proposes a methodology for development of protocol models. The methodology attempts to employ two modeling environments in models development, NS2 and DEVSim++, which will interoperate during simulation. NS2 is a widely used network simulator in protocol research, which employs an informal modeling approach. Within the approach time and state information of protocol models are not explicitly described, thus being hard to validate model. On the other hand the DEVS formalism is a mathematical framework for modeling a discrete event system in a hierarchical, modular manner. In DEVS, model's time and state information is described explicitly, By using DEVS formalism, models can easily be validated and errors in the modeling stage can be reduced. However, the DEVS simulator, DEVSim++, supports a small amount of models library which are required to build simulation models of general communication network. Although NS2 employs an informal modeling approach and models validation is difficult, it supports abundant models library validated by experimental users. Thus, combination of DEVS models and NS2 models may be an effective solution for network modeling. Such combination requires interoperation between DEVSim++ simulator and NS2 simulator. This paper develops an environment for such interoperation. Correctness and effectiveness of the implemented interoperation environment have been validated by simulation of UDP and TCP models.

• 한국정보처리학회:학술대회논문집
• /
• 한국정보처리학회 2008년도 춘계학술발표대회
• /
• pp.841-844
• /
• 2008

본 논문은 공개 네트워크 시뮬레이터 ns-2.31(Network Simulator 2.31)의 802.11 DCF 모듈에서 버그(bug)를 소개하고 이의 영향 결과를 분석한다. ns의 802.11 DCF 모듈은 다음과 같은 문제점을 가지고 있다. 첫째, 백오프(backoff) 알고리즘은 표준안에서 명시한 알고리즘대로 작성되지 않았다. 둘째, 특정조건에 해당되는 충돌에 대하여 트레이스 파일에 출력하지 않는다. 셋째, 전파 오류 모듈을 삽입하여도 전파 오류 결과를 트레이스 파일에 출력하지 않는다. 넷째, MAC(Medium Access Control) 알고리즘만을 평가할 수 있는 기법을 제공하지 않는다. 이러한 문제점을 수정한 ns-2와 수정전의 ns-2와 평균4.6%의 충돌률 차이를 보인다.

• BMB Reports
• /
• 제37권6호
• /
• pp.741-748
• /
• 2004

The hepatitis C virus is associated with the development of liver cirrhosis and hepatocellular carcinomas. Among the 10 polyproteins produced by the virus, no function has been clearly assigned to the non-structural 5A (NS5A) protein. This study was designed to identify the hepatocellular proteins that interact with NS5A of the HCV. Yeast two-hybrid experiments were performed with a human liver cDNA prey-library, using five different NS5A derivatives as baits, the full-length NS5A (NS5A-F, amino acid (aa) 1~447) and its four different derivatives, denoted as NS5A-A (aa 1~150), -B (aa 1~300), -C (aa 300~447) and D (aa 150~447). NS5A-F, NS5A-B and NS5A-C gave two, two and 10 candidate clones, respectively, including an AHNAK-related protein, the secreted frizzled-related protein 4 (SFRP4), the N-myc downstream regulated gene 1 (NDRG1), the cellular retinoic acid binding protein 1 (CRABP-1), ferritin heavy chain (FTH1), translokin, tumor-associated calcium signal transducer 2 (TACSTD2), phosphatidylinositol 4-kinase (PI4K) and $centaurin{\delta}$ 2 ($CENT{\delta}2$). However, NS5A-A produced no candidates and NS5A-D was not suitable as bait due to transcriptional activity. Based on an in vitro binding assay, CRABP-1, PI4K, $CENT{\delta}2$ and two unknown fusion proteins with maltose binding protein (MBP), were confirmed to interact with the glutathione S-transferase (GST)/NS5A fusion protein. Furthermore, the interactions of CRABP-1, PI4K and $CENT{\delta}2$ were not related to the PXXP motif (class II), as judged by a domain analysis. While their biological relevance is under investigation, the results contribute to a better understanding of the possible role of NS5A in hepatocellular signaling pathways.

• 동의생리병리학회지
• /
• 제29권2호
• /
• pp.180-188
• /
• 2015

This study was conducted to investigate the relaxation effects and its mechanisms of Nelumbinis Semen(NS) extract in isolated rabbit corpus cavernous tissues. In order to examine the relaxation effects and its mechanisms of NS, we treated the ethanol extract of NS(0.01-3.0 mg/ml) and indomethacin(IM), tetraethylammonium chloride(TEA), Nω -nitro-L-arginine (L-NNA), methylene blue(MB) were treated before NS extract to contracted strips induced by PE 1 μM. We also treated calcium chloride(Ca) 1 mM after pretreatment of NS extract in Ca²⁺-free krebs-ringer solution to contracted strips induced by PE. Cell viability and NO concentration on human umbilical vein endothelial cell(HUVEC) was measured by MTT assay, Griess reagent system. eNOS production was investigated by histochemical and immunohistochemical staining. NS extract was significantly affected on the relaxation of cavernous strips and NS extract-induced relaxation was not different by pretreatment of IM, TEA, MB, but inhibited by the pretreatment of L-NNA. And increase of contraction induced by Ca²⁺ addition, in a Ca²⁺-free solution, was decreased by pretreatment of NS. NO concentration on HUVEC was increased. When NS extract was applicated on corpus cavernosum of penis(CCP) in SHR, ratio of smooth muscles to collage fibers by PE was decreased and formation of eNOS around helicine artery was increased. These results suggest that CCP relaxation effects of NS extract are shown by suppressing influx of extracellular Ca²⁺ through the production of NO and eNOS.

• The Korean Journal of Physiology and Pharmacology
• /
• 제12권4호
• /
• pp.193-197
• /
• 2008

The influence of alpha-fetoprotein (AFP) on the bone marrow (BM) natural suppressor (NS) cells of intact Ehrlich carcinoma -bearing CBA mice was studied. Bone marrow NS cells were fractionated into three fractions by isopycnic centrifugation on percoll gradients: NS1 (${\rho}$=1.080 g/ml), NS2 (${\rho}$=1.090 g/ml) and NS3 (1.100> ${\rho}$ > 1.090 g/ml). These fractions were highly different in their sensitivity to known NS cell inductors (interleukin (IL)-2, IL-3 or histamine). None of the NS fractions isolated from the intact mice spontaneously produced antiproliferative activity, however, they showed a high level of NS (antiproliferative and natural killer cell inhibitory) activity under the influence of AFP. A single injection of AFP to intact mice led to an increase of spontaneous NS activity and the inhibition of natural killer cell activity. NS activity, especially NS2, was increased in when tumor cells were subcutaneously inoculated three days after AFP injection. In the AFP-treated mice, the tumor mass at 14 days was 60% larger than that in the untreated mice. Our data confirmed that AFP is a tumor marker that can inhibit cancer immunity and plays a role in cancer pathogenesis.

• 폴리머
• /
• 제36권1호
• /
• pp.111-116
• /
• 2012

메조포러스 물질의 표면을 post-synthesis grafting method를 통해 표면을 기능화시킨 후 $(n-BuCp)_2ZrCl_2$/methylaluminoxane(MAO)를 담지하여 에틸렌 중합을 실시하였다. 아민기와 시안기를 가지는 유기실란 $N$-[(3-trimethoxysilyl)propyl]ethylenediamine(2NS), 4-(triethoxysilyl)butyronitrile(1NCy), 1-(3-triethoxysilylpropyl)-2-imidazoline(2NIm)는 메조포러스 물질의 표면 기능화에 사용되었다. SBA-15/2NS/$(n-BuCp)_2ZrCl_2$촉매 담지시 MAO의 양이 증가할수록 Zr 함량은 감소하였고 Al 함량은 증가하였다. 에틸렌 중합 활성은 MAO의 양이 증가할수록 급격히 증가함을 볼 수 있었다. 담지시간이 6시간일 때 가장 높은 활성을 보였다. 유기실란의 종류에 따른 활성 차이는 SBA-15/2NS/$(n-BuCp)_2ZrCl_2$ > SBA-15/2NIm/$(n-BuCp)_2ZrCl_2$ > SBA-15/1NCy/$(n-BuCp)_2ZrCl_2$ 순으로 감소하였다. 아민기를 두 개 갖는 2NS와 2NIm은 아민기를 하나 갖는 1NCy보다 $(n-BuCp)_2ZrCl_2$와 더 강하게 상호작용을 한다. 따라서 촉매 내 질소와 Zr함량이 증가할수록 활성은 증가하였다.

• Molecules and Cells
• /
• 제40권3호
• /
• pp.202-210
• /
• 2017

The nonstructural protein 5A (NS5A) encoded by the human hepatitis C virus (HCV) RNA genome is a multifunctional phosphoprotein. To analyse the influence of NS5A on apoptosis, we established an Hep-NS5A cell line (HepG2 cells that stably express NS5A) and induced apoptosis using tumour necrosis factor $(TNF)-{\alpha}$. We utilised the MTT assay to detect cell viability, real-time quantitative polymerase chain reaction and Western blot to analyse gene and protein expression, and a luciferase reporter gene experiment to investigate the targeted regulatory relationship. Chromatin immunoprecipitation was used to identify the combination of $NF-{\kappa}B$ and miR-503. We found that overexpression of NS5A inhibited $TNF-{\alpha}$-induced hepatocellular apoptosis via regulating miR-503 expression. The cell viability of the $TNF-{\alpha}$ induced Hep-mock cells was significantly less than the viability of the $TNF-{\alpha}$ induced Hep-NS5A cells, which demonstrates that NS5A inhibited $TNF-{\alpha}$-induced HepG2 cell apoptosis. Under $TNF-{\alpha}$ treatment, miR-503 expression was decreased and cell viability and B-cell lymphoma 2 (bcl-2) expression were increased in the Hep-NS5A cells. Moreover, the luciferase reporter gene experiment verified that bcl-2 was a direct target of miR-503, NS5A inhibited $TNF{\alpha}$-induced $NF-{\kappa}B$ activation and $NF-{\kappa}B$ regulated miR-503 transcription by combining with the miR-503 promoter. After the Hep-NS5A cells were transfected with miR-503 mimics, the data indicated that the mimics could reverse $TNF-{\alpha}$-induced cell apoptosis and blc-2 expression. Collectively, our findings suggest a possible molecular mechanism that may contribute to HCV treatment in which NS5A inhibits $NF-{\kappa}B$ activation to decrease miR-503 expression and increase bcl-2 expression, which leads to a decrease in hepatocellular apoptosis.