• Fisheries and Aquatic Sciences
• /
• 제4권4호
• /
• pp.186-191
• /
• 2001

Vitellogenin (VTG) and VTG mRNA induction by $estradiol-17\beta\;(E_2)$ were examined in the primary cultures of hepatocyte in the rainbow trout. Hepatocytes were precultured for 2 days, then $E_2$ was added and cultured for another 5 days. Media and hepatocytes were then analyzed by electrophoresis and Northern blotting for VTG and VTG mRNA, respectively. The hepatocytes were formed a few aggregates within 5 days without further spreading to a monolayer. Cell viability and high DNA content were maintained during the incubation. The hepatocyte culture with E2 induced a weak VTG band at a molecular weight of 175kDa on Day 2 after $E_2$ addition. The relative amount of VTG was expressed in percentage of total protein concentrations. VTG was gradually increased as $1.9\%$ on Day 2, $6.3\%$ on Day 4 and $7.3\%$ on Day 5. VTG mRNA band was detected at about 6.6 kb in the culture with $E_2$ at day 1 of culture. The level of VTG mRNA expression linearly increased with time until Day 5 (r=0.97).

• Molecules and Cells
• /
• 제20권1호
• /
• pp.97-104
• /
• 2005

To investigate apoptosis in HC11 mammary epithelial cells, we compared the gene expression profiles of actively growing and serum-starved apoptotic cells using a mouse apoptosis gene array and $^{33}P$-labeled cDNA prepared from the RNA of the two cultures. Analysis of the arrays showed that expression of several genes such as clusterin, secreted frizzled related protein mRNA (sFRP-1), CREB-binding protein (CBP), and others was higher in the apoptotic cells whereas expression of certain genes including survivin, cell division cycle 2 homolog A (CDC2), and cyclin A was lower. These expression patterns were confirmed by RT-PCR and/or Northern analyses. We compared the expression of some of these genes in the mouse mammary gland under various physiological conditions. The expression levels of genes (clusterin, CBP, and M6P-R) up-regulated in apoptotic conditions were higher at involution than during lactation. On the other hand, genes (Pin, CDC2) downregulated in apoptotic conditions were relatively highly expressed in virgin and pregnant mice. We conclude that certain genes such as clusterin, sFRP-1, GAS1 and CBP are induced in apoptotic mammary epithelial cells, and others are repressed. Moreover, the apoptosis array is an efficient technique for comparing gene expression profiles in different states of the same cell type.

• The Plant Pathology Journal
• /
• 제15권1호
• /
• pp.8-13
• /
• 1999

Hypersensitive cell death of plants during incompatible plant-pathogen interactions is one of the efficient defense mechanisms of plants against pathogen infections. For better understanding of the molecular mechanisms involved in the plant hypersensitive response (HR), TMV-induced biotic plant cell death and CuSO4-induced abiotic plant cell death were compared in terms of expression patterns of ten different defense-related genes as molecular markers. The genes include five pathogenesis-related protein genes, two plant secondary metabolite-associated genes, two oxidative stress-related genes and one wound-inducible gene isolated from tobacco. Northern blot analyses revealed that a same set of defense-related genes was induced during both biotic and abiotic cell death but with different time and magnitude. The expression of defense-related genes in tobacco plants was temporarily coincided with the time of cell death. However, when suspension cell cultures was used to monitor the expression of defense-related genes, different patterns of the gene expression were detected. This result implies that three are common and, in addition, also different branches of signaling pathways leading to the induced expression of defense-related genes in tobacco during the pathogen- and heavy metal-induced cell death.

• The Plant Pathology Journal
• /
• 제22권4호
• /
• pp.318-322
• /
• 2006

One hundred and nine Agrobacterium isolates were recovered from 68 samples(51 plant tumor and 17 soil) that were collected from different habitats in Northern Jordan. The isolated cultures were grouped into 3 biovars based on their biochemical characteristics and biovar I, II, and III comprised a total number of 46, 41, and 22 isolates, respectively. Isolates of biovar I were obtained primarily from the diseased peach, oak and rose plants, whereas isolates of biovar II and ill were obtained mostly from apple and grape plants, respectively. Twenty-nine isolates were found to be virulent to at least one of the tested hosts such as carrots, chickpeas, garden peas and tomato plants with a response of tumor formation or tumor with roots induction. Our result suggested that A. tumefaciens strains from tumor of various plants and soil of Jordan were diverse and they have a variation in their virulence.

• KIEAE Journal
• /
• 제14권1호
• /
• pp.5-13
• /
• 2014

The ancestors of China created various proper dwellings in the vast Chinese lands, according to distinct geographical environment, climate, and special local products. The purpose of this study is to compare features of 7 typical traditional Chinese dwellings in different areas from historical, geographical, structural and tinctorial aspects, in order to see what the differences are and rearrange them by these 4 different scales as estimate standards. By Comparing features between 7 traditional dwellings, advantages and disadvantages of them were found out. Not only natural terrain but also cultural mentality is an important factor that effected on the changes of regionality. The result showed that historical length in a certain extent reflected the differences between dwellings in different areas; there are more regular type dwellings in the northern China; dwellings in different structural material types following their regions; and, southern dwellings prefer natural and simple exterior colors. Also From construction aesthetics and culture perspective, Different cultures have cultivated and brought out different dwelling styles. Thus, protecting on traditional dwellings is of great necessities and this study will bring benefits in many aspects. For a further research, the result of this study can be utilized as the base data that suggest directions for effective Chinese residence planning in different areas meeting different demands.

• Journal of Ecology and Environment
• /
• 제35권2호
• /
• pp.141-147
• /
• 2012

The rapid urbanization in developing countries is accelerating both the depletion and fragmentation of urban green space, despite the known positive effects of green spaces on the environmental conditions in cities and the quality of life of residents. Consequently, there is a need for practical tools that can support the development of networks of urban green spaces. This article presents a study that used a GIS-based least cost path (LCP) analysis to identify the best alternative for developing an urban green space network in the coastal city of Jakarta, Indonesia, which was based on the evaluation of topography and land use characteristics. Pair-wise analysis was used to reduce the sensitivity in the LCP model. The results showed that the coastal wetlands in the northern part of Jakarta and the agricultural fields in the suburban areas of Jakarta play an important role in connecting the green space network. On the other hand, some green spaces in the central part of Jakarta could not be connected by the LCP model. The method used in this study can serve as a tool to support the identification of networks of potential urban green spaces. It can also provide useful information for sustainable urban landscape planning and management in urban ecosystems. However, the inclusion of socio-economic criteria would further improve the model.

• 한국의류산업학회지
• /
• 제16권5호
• /
• pp.698-705
• /
• 2014

This study classifies features of women's hair adornments with the functional and decorative aspects in the Three Kingdoms Period and analyses in regards to their contemporary social and cultural surroundings. First, the tree-shaped and Rod-like hairpins in the Three Kingdoms Period are not as similar as to the hairpins found in China or other northern countries. It is farming lives and the wood-worship religion established the wearing of high-bun hair styles and tree-shaped hairpins. Second, high-bun hair styles and -shaped hairpins were basic hair decorations during the Three Kingdoms Period. Common high-bun styles finally became one of the cultures that reflected the contemporary thinking. High-bun hair styles, not excessively-decorated, became harmonized with the costume beauty and later developed along with with various-shaped hairpins. Third, Buyao (made of metals and based on flower subjects)in the Three Kingdoms Period was a unique style not been seen in the Han and Wei-jin-nam-bei-chao Period. Flowers in Buyao of the of the Three Kingdoms Period show an elegant sway, which is not excessively-decorated that developed into unique features.

• 한국식물학회:학술대회논문집
• /
• 한국식물학회 1999년도 제13회 식물생명공학심포지움 New Approaches to Understand Gene Function in Plants and Application to Plant Biotechnology
• /
• pp.45-49
• /
• 1999

Plant cell culture is emerging to express bioactive foreign proteins because it has several advantages in that it is safe, economical, genetically stable and eukaryotic expression system comparing with other expression systems. However several limitations such as slow growth rate, low expression level and lack of well established down stream process need to be answered. As a preliminary approach to produce the immunologically interested molecules through the plant cell culture, we tested if granulocyte-macrophage colony stimulating factors (GM-CSFs) from both murine (mGM-CSF) and human (hGM-CSF) are produced as a biologically active form through plant cell culture. The murine and human GM-CSF genes were cloned into the plant expression vector, pBI121, and Ti-plasmid mediated transformation of tobacco leaves was conducted using Agrobacterium tumefaciens harboring both recombinant GM-CSF (rGM-CSF) genes. Cell suspension culture was established from the leaf-derived calli of transgenic tobacco plant. Northern blot analysis indicated the expression of the introduced mGM-CSF gene in both transgenic plant and cell suspension cultures. In addition, the biological activities of both murine and human GM-CSF from plant cell culture were confirmed by measuring the proliferation of the GM-CSF dependent FDC-PI and TF-1 cells, respectively.

• 한국육종학회지
• /
• 제42권5호
• /
• pp.455-462
• /
• 2010

Purple acid phosphatase is important for phosphorus remobilization in plants, but its role in plant adaptation to low phosphorus availability is not known. The cDNA encoding O. sativa purple acid phosphatase (OsPAP1) has 1008 bp with an open reading frame of 335 amino acid residues. The amino acid sequence of OsPAP1 cDNA shows of 50-51% identity with other plant purple acid phosphatases. OsPAP1 was expressed in rice plants and in cell cultures in the absence of phosphate ($P_i$). The expression was organ-specific with the strongest expression in $P_i$-deprived roots. Functional expression of the OsPAP1 gene in the transgenic Arabidopsis line was confirmed by northern and western blot analysis. OsPAP1 overexpression lines had higher phosphatase activity than wild-type. Overexpression of OsPAP1 in Arabidopsis plants resulted in increased Pi accumulation under Pi sufficient condition. These results show that the OsPAP1 gene represents more efficient $P_i$ uptake and can be used to develop new transgenic dicotyledonous plants.

• 수완나부미
• /
• 제11권2호
• /
• pp.25-57
• /
• 2019

The paper addresses Erik Cohen's pioneering work on tourism in Thailand, specifically his publications on the relations between Thai women and foreign (farang) men in tourist-oriented encounters. Of sociological-anthropological interest is his conceptualization of these relations as "open-ended prostitution as a skilful game of luck" based on his study of a Bangkok soi (lane) in 1981-1984, and his exploration of Thai culture in terms of ambiguity and contradiction. On the basis of recent ethnographic research in the northern Thai tourist hub of Chiang Mai and wide-ranging observations on tourism development in Thailand, we examine continuity and change in these male-female engagements since Cohen's research, especially in the context of the increasing availability of such electronic agencies as social media, messaging, video chat, and internet dating. Whereas Cohen's concept of ambiguity and illusion has tended to disappear from physical spaces, it seems to have resurfaced in virtual space. The complexities of host-guest relations, and particularly the interactions both within the variegated category of "guests" themselves and then between their "hostesses" are explored in terms of sites of tourism-oriented encounters in both physical and virtual space so as to deconstruct these oppositional categories which have been formative in studies of tourism.