• Journal of Microbiology and Biotechnology
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• v.24 no.12
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• pp.1744-1754
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• 2014

The hepatitis C virus (HCV) RNA genome is replicated by an RNA replicase complex (RC) consisting of cellular proteins and viral nonstructural (NS) proteins, including NS5B, an RNA-dependent RNA polymerase (RdRp) and key enzyme for viral RNA genome replication. The HCV RC is known to be associated with an intracellular membrane structure, but the cellular components of the RC and their roles in the formation of the HCV RC have not been well characterized. In this study, we took a proteomic approach to identify stomatin, a member of the integral proteins of lipid rafts, as a cellular protein interacting with HCV NS5B. Co-immunoprecipitation and co-localization studies confirmed the interaction between stomatin and NS5B. We demonstrated that the subcellular fraction containing viral NS proteins and stomatin displays RdRp activity. Membrane flotation assays with the HCV genome replication-competent subcellular fraction revealed that the HCV RdRp and stomatin are associated with the lipid raft-like domain of membranous structures. Stomatin silencing by RNA interference led to the release of NS5B from the detergent-resistant membrane, thereby inhibiting HCV replication in both HCV subgenomic replicon-harboring cells and HCV-infected cells. Our results identify stomatin as a cellular protein that plays a role in the formation of an enzymatically active HCV RC on a detergent-resistant membrane structure.

• Journal of the Korea institute for structural maintenance and inspection
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• v.7 no.3
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• pp.139-146
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• 2003

The objective of the presented study is to develop an efficient procedure of proof load testing for existing bridges. By analytical methods, some of these bridges are not adequate to carry normal highway traffic. However, the actual load carrying capacity is often much higher than what can be determined by conventional analysis. Proof load testing can reveal the hidden strength reserve and thus verify the adequacy of the tested bridge. Proof load level required for meaningful tests should be sufficiently higher than legal load. In the state of Michigan, the legal 11-axle truck can weigh up to 685 kN. In this study, a combination of two military tanks and two Michigan 11-axle trucks was used. The proof loads were gradually increased to ensure the safety of the test. After each move, measurements were taken. For the considered bridge, stress levels were rather low compared to pre-test analysis results. This is due to incorrect material strength, structural contribution of nonstructural components such as parapets and railings, and partially fixed supports.

• Annals of Rehabilitation Medicine
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• v.42 no.6
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• pp.863-871
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• 2018

Objective To evaluate the association between progression of curvature of scoliosis, and correction for functional component in patients with juvenile idiopathic scoliosis (JIS). Methods We retrospectively reviewed medical data of patients prescribed custom molded foot orthosis (FO) to correct inequality of RCSPA (resting calcaneal stance position angle), and chose 52 patients (26 females, 26 males) with Cobb angle ${\geq}10^{\circ}$ in radiology and uneven pelvic level at iliac crest by different RCSPA (${\geq}3^{\circ}$) as a factor of functional scoliosis. They had different hump angle ${\geq}5^{\circ}$ in forward bending test, for idiopathic scoliosis component. Their mean age and mean period of wearing FO were $79.5{\pm}10.6months$ and $18.6{\pm}0.70months$. Results Cobb angle was reduced from $22.03^{\circ}{\pm}4.39^{\circ}$ initially to $18.86^{\circ}{\pm}7.53^{\circ}$ after wearing FO. Pelvis height difference and RCSPA difference, were reduced from $1.07{\pm}0.25cm$ initially to $0.60{\pm}0.36$, and from $4.25^{\circ}{\pm}0.71^{\circ}$ initially to $1.71^{\circ}{\pm}0.75^{\circ}$ (p<0.01). Cobb angle improved most in 9 months. However, there was no significant improvement for those with more than $25^{\circ}$ of Cobb angle initially. Mean Cobb angle improved in all age groups, but patients less than 6 years had clinically significant improvement of more than $5^{\circ}$. Conclusion JIS can have functional components, which should be identified and managed. Foot orthosis is useful in correcting functional factors, in the case of pelvic inequality caused by different RCSPA, for patients with juvenile idiopathic scoliosis.

• Journal of The Korean Society of Grassland and Forage Science
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• v.6 no.3
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• pp.157-163
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• 1986

Synthesis and accumulation pattern of Weender components in orchardgrass (Dactylis glomerata L.) cv. Potomac and Baraula, perennial ryegrass (Lolium perenne L.) cv. Reveille and Semperweide and meadow fescue (Festuca pratensis Huds.) cv. Cosmos 11 and N.F.G. were studied under different growth environments and cutting managements. The field experiments were conducted as a split plot design with three cutting regimes of 6-7 cuts at grazing stage, 4-5 cuts at silage and 3 cuts at hay stage from 1975 to 1979 in Korea and West Germany. The results obtained are summarized as follows: 1. Air temperature, rainfalls and solar radiation were found to be an important meteorological factors influenced to synthesis and accumulation of Weender components. Under high temperature and strong solar radiation during summer season in Korea, accumulation of crude fiber and cell-wall constituents (NDF) in the plants, as average of all grass species and cutting regimes, were increased to about 30.1% and 48.7% from 27,9% and 42.9% in spring, respectively, while total nonstructural carbohydrates (TNC) were decreased to 1.52% in summer from 4.01% in spring. In West Germany, the concentration of Weeder components showed little seasonal variation. 2. Crude fiber and neutral detergent fiber (NDF) were shown higher concentration in orchardgrass than those of perennial ryegrass and meadow fescue, but N-free extractions and TNC as well as net energy value were less accumulated in orchardgrass. Orchardgrass contained lower net energy contents with 534 StE. 431 StE and 575 StE/kg for Suweon, Cheju and Freising, respectively, as compared with 624 StE (Suweon), 491 StE (Cheju) and 657 StE/kg (Freising) in meadow fescue.

• Journal of Korea Water Resources Association
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• v.39 no.2 s.163
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• pp.161-178
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• 2006

Heathcote (1998) identified a systematic, seven-step approach to general watershed planning and management. It consists of 1) understanding watershed components and processes, 2) identifying and ranking problems to be solved, 3) setting clear and specific goals, 4) developing a list of management options, 5) eliminating infeasible options 6) testing the effectiveness of remaining feasible options, and 7) developing the final options. In this study the first five steps of that process were applied to the Anyangcheon watershed in Korea, which experiences streamflow depletion, frequent flood damages, and poor water quality typical of highly urbanized watersheds. This study employed four indices: Potential Flood Damage(PFD), Potential Streamflow Depletion(PSD), Potential Water Quality Deterioration(PWQD) and Watershed Evaluation Index(WEI) to identify and quantify problems within the watershed. WEI is the integration index of the others. Composite programming which is a method of multi-criteria decision making is applied for the calculation of PSD, PWQD and WEI (Step 2). The primary goal of the study is to secure instreamflow in the Anyangcheon during dry seasons. The second management goals of flood damage mitigation and water quality enhancement are also set (Step 3). Management options include not only structural measures that can alter the existing conditions, but also nonstructural measures that rely on changes in human behavior or management practices (Step 4). Certain management options which are not technically, economically, and environmentally feasible, are eliminated (Step S). Therefore, this study addresses a Pre-feasibility study, which established a master plan using Steps 1 through 5.