• Animal cells and systems
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• 제16권6호
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• pp.431-437
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• 2012

Calpains are a class of proteins that belong to the calcium-dependent, non-lysosomal cysteine proteases. There are three major types of calpains expressed in the skeletal muscle, namely, ${\mu}$-calpain, m-calpain, and calpain 3, which show proteolytic activities. Skeletal muscle fibers possess all three calpains, and they are $Ca^{2+}$-dependent proteases. The functional role of calpains was found to be associated with apoptosis and myogenesis. However, calpain 3 is likely to be involved in sarcomeric remodeling. A defect in the expression of calpain 3 leads to limb-girdle muscular dystrophy type 2A. Calpain 3 is found in skeletal muscle fibers at the N2A line of the large elastic protein, titin. A substantial proportion of calpain 3 is activated 24 h following a single bout of eccentric exercise. In vitro studies indicated that calpain 3 can be activated 2-4 fold higher than normal resting cytoplasmic [$Ca^{2+}$]. Characterization of the calpain system in the developing muscle is essential to explain which calpain isoforms are present and whether both ${\mu}$-calpain and m-calpain exist in differentiating myoblasts. Information from such studies is needed to clarify the role of the calpain system in skeletal muscle growth. It has been demonstrated that the activation of ubiquitous calpains and calpain 3 in skeletal muscle is very well regulated in the presence of huge and rapid changes in intracellular [$Ca^{2+}$].

• BMB Reports
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• 제48권1호
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• pp.48-53
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• 2015

Oxidized LDL (oxLDL) performs critical roles in atherosclerosis by inducing macrophage foam cell formation and promoting inflammation. There have been reports showing that oxLDL modulates macrophage cytoskeletal functions for oxLDL uptake and trapping, however, the precise mechanism has not been clearly elucidated. Our study examined the effect of oxLDL on non-muscle myosin heavy chain IIA (MHC-IIA) in macrophages. We demonstrated that oxLDL induces phosphorylation of MHC-IIA (Ser1917) in peritoneal macrophages from wild-type mice and THP-1, a human monocytic cell line, but not in macrophages deficient for CD36, a scavenger receptor for oxLDL. Protein kinase C (PKC) inhibitor-treated macrophages did not undergo the oxLDL-induced MHC-IIA phosphorylation. Our immunoprecipitation revealed that oxLDL increased physical association between PKC and MHC-IIA, supporting the role of PKC in this process. We conclude that oxLDL via CD36 induces PKC-mediated MHC-IIA (Ser1917) phosphorylation and this may affect oxLDL-induced functions of macrophages involved in atherosclerosis.

• 한국산업보건학회지
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• 제32권3호
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• pp.221-230
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• 2022

Objectives: The aim of this study is to evaluate the association between shiftwork and skeletal muscle mass index in a single university health check-up. Methods: We used data from 98,227 workers who answered in a special interview on health check-up at a local university hospital from 2014 to 2020. Pearson correlation analysis was conducted for comparing the association between skeletal muscle mass index and demographic and hematological variables in shiftwork and non-shiftwork groups. Mixed linear model analysis after controlling demographic and hematological variables was used to analyze the difference of skeletal muscle mass index between groups at every visit for seven years. Results: In linear regression analysis, the variables most significantly correlated with skeletal muscle index in both groups were shiftwork(p=0.049), BMI(p<0.001), hypertension(p=0.024), platelet(p<0.001), total protein (p<0.001), AST(p=0.028), ALT(p=0.003), ALP(p<0.001), total cholesterol(p=0.002), triglyceride(p=0.019), BUN (p=0.001), creatinine(p<0.001), and uric acid(p=0.002). After the adjustment for demographic and hematologic variables, the skeletal muscle mass index at every visit was decreased both in the shiftwork group and non-shiftwork group. The slope of the shiftwork group was -0.240 and non-shiftwork group -0.149, showing a significant difference (p<0.001). Conclusions: In the shiftwork group, the skeletal muscle mass index showed a tendency to decrease markedly over time compared to the non-shiftwork group. It is presumed that shift workers' skeletal muscle health was adversely affected by changes in the biological clock due to changes in wake-up and sleep patterns, and changes in food intake.

• 한국식품과학회지
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• 제21권3호
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• pp.364-369
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• 1989

본 실험은 냉동저장 중에 법동변성 방지제의 첨가가 우육과 돈육의 품질에 미치는 영향을 알아본 것으로 냉동변성 방지제 종류는 sorbitol, mono sodium glutamate와 sodium tripolyphosphate를 혼합하여 사용한 CP-A, sorbitol, sucrose와 sodium tripolyphosphate를 섞은 CP-B 그리고 sorbitol, sucrose, sodium citrate와 sodium tripolyphosphate를 사용한 CP-C세 종류이었고 시료는 $-20^{\circ}C$에서 12주간 저장되었으며 분석항목으로는 pH변화, TBA가, 유리지방산 함량의 변화, 수용성 및 염용성 단백질의 추출성이었다. 그 결과를 요약하면 다음과 같다. 1. 냉동변성 방지제 첨가구의 pH는 무첨가구의 pH보다 0.2-0.5unit 정도의 상승효과를 보였으며 TBA가 및 유리지방산 함량은 저장기간이 길어짐에 따라 무첨가구에 비해 증가하는 경향을 보였다. 2. 수용성 단백질의 추출성은 냉동변성 방지제를 처리할 때 돈육이 우육에 비해 높은 추출율을 보였는데 특히 돈육의 CP-B 처리구에서 무첨가구에 비해 우수하였다. 3. 염용성 단백질의 추출성에 대한 냉동변성 방지제의 효과는 우육의 CP-A 처리구와 돈육의 CP-B 처리구에서 무첨가구에 비해 약 10-25%의 높은 추출성 향상을 나타내었다.

• 한국식품영양과학회지
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• 제32권7호
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• pp.1032-1038
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• 2003

전갱이 육으로 제조한 알칼리 수리미의 가열 겔 강도 증강을 위한 최적 전분 및 비근육 단백질의 선정과 이들 성분의 최적 첨가량을 설정하기 위해 2수준 fractional factorial과 mixture design을 실시하였으며, 반응 값은 punch test에 의한 물성과 색차계로 색을 측정하였다. 감자, 옥수수 및 밀전분은 파괴 강도 값을 감소시키지만 변형 값에는 유의적인 영향을 미치지 않는 것으로 나타났다. 소혈청 단백질은 파괴강도 값을 크게 증가시킨 반면 분말 난백 단백질은 다소 효과가 인정되었고 유장단백질과 대두 농축 단백질은 효과가 없었다. 그리고 소 혈청 단백질은 변형값을 다소 증가시킨 반면 분말 난백 단백질을 크게 감소시켰다. 감자와 옥수수 전분과 비근육 단백질은 백색도를 다소 개선하였다. 파괴강도 110g 이상, 변형 값 4.2 mm이상, 백색도 22.5 이상을 보이는 수리미, 옥수수전분 및 소혈청의 최적 혼합 비율은 각각 89.5∼90.0%, 4.6∼6.0%, 4.3∼5.4%의 범위였다. 전분과 비근육 단백질을 첨가하여 제조한 전갱이 알칼리 수리미의 가열 겔이 수세 수리미 겔에 비하여 균일한 단백질 분포를 보이고 있었다.

• Nutrition Research and Practice
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• 제13권1호
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• pp.17-22
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• 2019

BACKGROUND/OBJECTIVES: Successful recovery of an animal from exercise is essential, especially prior to the next exercise session. This study was conducted to find an effective exercise-to-rest period ratio for the restoration of energy sources and replenishment of anti-oxidative status in tissue after exercise. MATERIALS/METHODS: Thirty-two rats were assigned to either non-training or training exercise groups for 5 weeks. After that period, the two groups were subdivided into four smaller groups: non-exercise (NE), exercise 0.5 hour and rest 1 hour (ER0.5:1), exercise 1 hour and rest 1 hour (ER1:1), exercise 2 hours and rest 1 hour (ER2:1). RESULTS: In the training group animals and compared to the NE group, the levels of plasma glucose after the rest period were significantly high in all ER groups but highest in the ER2:1 group. Similarly, the liver glycogen level was highest in the ER2:1 group. The plasma FFA level reached the highest level in the ER2:1 group but was similarly high in the ER0.5:1 group. Liver TG level was unchanged in the ER2:1 and ER1:1 groups but was significantly high in the ER0.5:1 group. Muscle TG levels were decreased in all three ER groups. Plasma protein levels were significantly high in the ER2:1 and ER0.5:1 groups. In both training animal and non-training animals, the liver protein levels did not change significantly between the NE and ER groups, irrespective of the exercise-to-rest ratio. In the training animal group, muscle protein level was significantly low in the ER2:1 and ER0.5:1 groups. The activity levels of superoxide dismutase and catalase, as well as the malondialdehyde concentration, were not significantly different between NE and ER groups, irrespective of the exercise-to-rest period ratio. CONCLUSIONS: These results indicate that animals provided with a 0.5:1 to 1:1 exercise-to-rest period ratio can restore their muscle energy sources and recover their anti-oxidative defense system.

• Fisheries and Aquatic Sciences
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• 제20권7호
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• pp.13.1-13.7
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• 2017

In order to evaluate the maximal storable period of the raw crab for a non-thermal muscle separation, the quality changes of the leg meat of red snow crab (Chionoecetes japonicus) during freeze storage were investigated. Fresh red snow crabs were stored at $-20^{\circ}C$ for 7 weeks, and the leg muscle was separated by a no heating separation (NHS) method every week. During the storage, considerable loss of the leg muscle did not occur and microbiological risk was very low. In contrast, discoloration appeared at 2-week storage on around carapace and the leg muscle turned yellow at storage 3-week. In physiochemical parameters, protein and free amino acids gradually decreased with storage time, expected that proteolytic enzymes still activated at $-20^{\circ}C$. At 4-week storage, the sensory acceptance dropped down below point 4 as low as inedible and notable inflection points in pH and acidity were observed. The volatile base nitrogen was low, though a little increase was recorded. These results suggested that the maximal storable period at $-20^{\circ}C$ of the raw material was within 2 weeks and it was depended on external factor such as the discoloration. The present study might be referred as basic data for approaches to solve quality loss occurred in non-thermal muscle separation.

• 한국어류학회지
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• 제26권3호
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• pp.171-178
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• 2014

로즈마리의 주요 성분인 carnosic acid는 carnosol, rosmarinic acid, ursolic acid 등과 같은 폴리페놀의 한 성분으로 다양한 생리활성 기능이 보고되어 있다. 본 연구에서는 로즈마리 유래 폴리페놀인 carnosic acid가 제브라피쉬 근육성장에 미치는 영향을 근육 내 주사와 사료를 통해서 확인해 보았다. 근육 내 주사 실험을 통해서 CA는 제브라 피쉬의 근육 내 단백질 함량을 증가시키고 중성지방의 함량을 감소시켰다. 또한 조직학적 분석 결과 근섬유의 평균 면적이 커지는 근섬유의 과비대 효과를 나타내었다. 사료 실험 결과 근육 내 단백질 및 중성지방의 함량에는 영향을 미치지 않았으며 조직학적 분석 결과 근육 내 주사 실험에서와 마찬가지로 근 섬유의 과비대를 유도하였다.

• 대한임상전기생리학회지
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• 제1권1호
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• pp.73-85
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• 2003

The purpose of this study is to discuss and analyze the change of GLUT-4 and muscle fiber type of streptozotocin(STZ)-diabetic rats over a period of 6 weeks. We divided into four groups; I(aquatic exercise and feeding of Cordyceps militaris; n=6), test group II(feeding of Cordyceps miliaris; n=6), test group III(aquatic exercise; n=6), control group IV(non-treatment; n=6). After experimenting we measured the blood glucose, body weight, muscle fiber type and GLUT-4 protein content. The change of glucose levels decreased greater in group I than the other group. The body weight gain was lower in the all groups. The change femoris muscle fiber type, the size of muscle fiber TypeII lessened more than the one of Type I in group IV. Decrease of muscle fiber size more diminishment in group I than the other group. GLUT-4 protein quantity decrease in group IV compared to normal group. It was significantly increased in group. I, III compared to group IV. But there was more increase in group I (p<.001). These results suggest that GLUT-4 and muscle fiber type II decrease in STZ-diabetic rats and that when we apply aquatic exercise and feeding of Cordyceps militaris in diabetic rats over a period 6 weeks, it is increase GLUT-4 and the increase of insulin sensitivity of peripheral tissue. So it is considered to be helpful in improvement of glucose homeostasis and in prevent from muscle atrophy resulted from complication.

• 한국수산과학회지
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• 제43권2호
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• pp.109-116
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• 2010

This study investigated the effects of astaxanthin, which is used to improve the muscle color of fish, on the nutritional composition of rainbow trout (Oncorhynchus mykiss). The trout were fed extruded pellets containing astaxanthin (pigmented rainbow trout) or without astaxanthin (non-pigmented rainbow trout). No significant differences in the contents of crude protein and crude ash between the two muscles of the two groups were observed. However, the crude fat composition of the muscle of pigmented rainbow trout was about two times higher than that of control muscle. In the muscle of pigmented rainbow trout, the contents of docosahexaenoic acid (DHA, 22:6n-3) and eicosapentaenoic acid (EPA, 20:5n-3), which are functional polyunsaturated fatty acids, were 1.6 fold higher than in non-pigmented muscle. In addition, the contents of minerals such as Zn and Fe, vitamins (B group, C, and E), free amino acids, carotenoids and astaxanthin were increased in the muscle of rainbow trout fed pellets supplemented with astaxanthin. Specifically, the content of the bioactive compound astaxanthin, was increased six times in the pigmented muscle, as compared to the control muscle. The results suggest that a diet supplemented with astaxanthin improves the nutritional composition of rainbow trout muscle as well as improving the muscle color.