• Molecules and Cells
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• 제42권10호
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• pp.721-728
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• 2019

Non-structural protein 1 (NS1) of influenza virus has been shown to inhibit the innate immune response by blocking the induction of interferon (IFN). In this study, we isolated two single-stranded RNA aptamers specific to NS1 with $K_d$ values of $1.62{\pm}0.30nM$ and $1.97{\pm}0.27nM$, respectively, using a systematic evolution of ligand by exponential enrichment (SELEX) procedure. The selected aptamers were able to inhibit the interaction of NS1 with tripartite motif-containing protein 25 (TRIM25), and suppression of NS1 enabled retinoic acid inducible gene I (RIG-I) to be ubiquitinated regularly by TRIM25. Additional luciferase reporter assay and quantitative real-time PCR (RT-PCR) experiments demonstrated that suppression of NS1 by the selected aptamers induced IFN production. It is noted that viral replication was also inhibited through IFN induction in the presence of the selected aptamers. These results suggest that the isolated aptamers are strongly expected to be new therapeutic agents against influenza infection.

• Earthquakes and Structures
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• 제13권2호
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• pp.103-118
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• 2017

In vertical seismic isolation (VSI), a building is partitioned intentionally by vertical layers into two dynamically different substructures for seismic response reduction. Initially, a 1-story frame was partitioned into two substructures, interconnected by viscous and visco-elastic links, and seismic responses of the original and the vertically isolated structures (VIS) were obtained, considering a large number of stiffness and mass ratios of substructures with respect to the original structure. Color contour graphs were defined for presentation and investigation of large amounts of output results. Dynamic characteristics of the isolated structures were studied by considering the non-classical damping of the system, and then the effects of viscous and visco-elastic link parameters on the modal damping ratios were discussed. On this basis, three states of mass isolation, interactional state, and control mass were differentiated. Response history analyses were performed by Runge-Kutta numerical method. In these analyses, interaction of isolation ratios and link parameters, on response control of VIS was studied and the appropriate ranges for link parameters as well as the optimal ranges for isolation ratios were suggested. Results show that by using the VSI technique, seismic response reduction up to 50% in flexible substructure and even more in stiff substructure is achievable.

• 미생물학회지
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• 제55권3호
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• pp.283-285
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• 2019

이 연구에서는 Illumina Hiseq platform을 사용하여 동해 심층 해양수로부터 분리된 Pelagicola sp. DSW4-44 (= KCTC 62762 = KCCM 43261)의 초안 유전체 염기서열 해독을 수행하였다. 그 결과, 유전체는 대략 4.85 Mbp의 길이 및 54.3%의 G + C 함량으로 구성되었고, 전체 4,566개의 단백질 암호 유전자, 3개의 rRNA 유전자, 48개의 tRNA 유전자, 3개의 non-coding RNA 유전자 및 67개의 위유전자(pseudo gene)가 확인되었다. 초안 유전체에서 균주 DSW4-44는 Pelagicola 속의 다른 균주에서 발견되지 않는 이화적 질산염의 암모늄 환원과 탈질화의 질소대사 유전자를 가지고 있었다.

• 미생물학회지
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• 제55권2호
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• pp.149-151
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• 2019

Parvimonas micra는 그람 양성, 절대 혐기성, 비운동성 및 아포를 생성하지 않는 구균이다. 이 세균 종은 구강의 정상 세균 총 하나이며, 구강 감염성질환 및 전신질환고도 연관이 있다. P. micra KCOM 1037 (= ChDC B276) 균주가 수술후상악낭종 병소에서 분리되었다. 여기에서 P. micra KCOM 1037 균주의 유전체 염기서열을 완전 해독하여 보고한다.

• 미생물학회지
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• 제55권2호
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• pp.152-153
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• 2019

Prevotella denticola는 그람 음성, 절대 혐기성, 비운동성이면서 아포를 형성하지 않는 막대 모양의 세균이다. P. denticola는 치주질환과 관련이 있으며, 치주질환의 위험 인자 중 하나이다. P. denticola KCOM 1525 (= ChDC B698) 균주가 사람 치근단 농양에서 분리되었다. P. denticola KCOM 1525 균주의 유전체 염기서열을 완전 해독하여 보고한다.

• 미생물학회지
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• 제54권4호
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• pp.480-482
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• 2018

이 연구에서는 Illumina Hiseq platform을 사용하여 동해 심층 해양수로부터 분리된 Zhongshania marina $DSW25-10^T$의 유전체 염기서열 해독을 수행하였다. 그 결과, 유전체는 대략 4.08 Mbp의 길이 및 49.0%의 G + C 함량으로 구성되었고, 전체 3,702개의 단백질 암호 유전자, 3개의 rRNA 유전자, 39개의 tRNA 유전자, 4개의 non-coding RNA 유전자 및 36개의 위 유전자(pseudogenes)가 확인되었다. 또한, 지방족 및 방향족 화합물의 대사 경로가 확인되었다. 이러한 대사 경로들로 비추어 Zhongshania marina $DSW25-10^T$는 유용한 생물 정화 자원으로 사용될 수 있을 것으로 기대된다.

• 대한건축학회논문집:구조계
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• 제36권2호
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• pp.137-144
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• 2020

The purpose of this study is to experimentally evaluate the effect of improving seismic performance by applying the details of seismic reinforcement to the reinforced concrete frame with non-seismic details while maintaining the original opening shape. In this study, based on CF specimens with specific seismic details, a total of four full scale specimens were designed and fabricated. The main variables are the width and spacing of steel dampers installed in the upper and lower parts of seismic reinforcement details, and the presence or absence of torsion springs installed in the hinges. As a result of the test, it was evaluated to be helpful for seismic retrofit and opening isolation of steel dampers installed at the upper and lower parts of the seismic reinforcement details and torsion springs installed at the joints. In particular, CFR2S specimens with torsion springs showed the best performance in terms of strength, stiffness and energy dissipation capacity with increasing displacement angle.

• 대한수의학회지
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• 제40권3호
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• pp.505-514
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• 2000

This study was conducted to investigate the isolation prequency, serotypes, and related epidemiological properties of 341 Salmonella spp from domestic poultry and environmental samples during the period 1993-1995. A total of 1,918 samples was collected during the three years period in nationwide. Most of Salmonella spp were isolated from the intestinal contents of poultry, especially cecal(46.0%) and rectal(35.8%) contents. Among the tested samples, rat(28.5 %) was the most predominant Salmonella reservoirs and followed by duck(24.8%), broiler(18.8%), layer(14.8%) and feed(7.1%), in order. More than twelve Salmonella serovars were identified among the 341 Salmonella isolates. The most prevalent serotypes isolated from non-human sources were S enteritidis (22.3%) and S pullorum (21.9%), S muenchen (13.9%), S typhimurium (12.6%), S gallinarum, S meleagridis, S heidelberg, and S senftenberg were followed, in order. In layer chickens, S pullorum (26.0%) was the most predominant serotype but S muenchen (33.0%) was in broiler chickens, S enteritidis (28.4%) was in ducks, and S typhimurium (60.0%) was in rats, respectively. As a results, S enteritidis was identified as the most prevalent serotype in non-human Salmonella isolates in Korea during the period 1993-1995. A preliminary study on the phage typing of 19 S enteritidis selected from the nationwide scale was shown that S enteritidis phage type(PT) 4 was the most predominant PT, and SEPT 1, SEPT 6a, SEPT 7 and SEPT 7a variant were also found in the same period.

• 가정과삶의질연구
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• 제27권5호
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• pp.77-90
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• 2009

The purpose of this study was to investigate the residents' use and occupancy-behavior in the activity areas of the senior nursing facilities, and to provide basic information to establish the appropriate physical elements for planning the activity areas. For the study, the observations in five facilities were conducted for one day, from 10 a.m. to 4 p.m by four researchers. The results of the study are summarized as follows: First, most of the using behaviors in the activity areas were the doing nothing or sleeping. The meals and program services were provided in only one activity area of the floor and it showed that the unit care system was perfunctorily conducted at those facilities. In the representative activity area, its openness was the main physical element influencing the spatial using frequency, while the accessibility and the openness in the sub-activity area were most important. The seating arrangements having comers were helpful for residents' interactions. Second, while facility programs and meals were provided in the specific activity area, there was no residents' occupancy in other activity areas at the same time. There were interactions including residents' conversations and watching/observations in non-designated activity areas such as the nursing stations and near corridors. But the residents' interactions and self-regulations were blocked by absence of territoriality, monotonous spatial compositions and furniture arrangements, insecurity of residents' privacy, wide or narrow areas, and isolated spatial type. Based on the results at the above, basic guidelines for planning the activity areas of senior nursing facilities can be proposed as follows: First, the isolated type and the sight interception should be avoided in representative activity areas. It should be partitioned with couple of areas through the appropriate furniture arrangements, and be prepared semi-private spaces in non-designated areas such as nursing station for the interactions among the residents and the staff. Second, in activity areas for small group, the isolated type is not also good for the residents' accessibility. The residents' privacy should be confirmed through the various spatial compositions, and enough areas need to be sure for the diverse furniture arrangements.

• Journal of Veterinary Science
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• 제23권1호
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• pp.6.1-6.8
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• 2022

Background: With limited information available, the association among urinary tract infections, urease-producing bacteria and the presence of magnesium ammonium phosphate (MAP) urolithiasis in canines in Thailand requires more study. Objectives: This study aimed to investigate the association between demographic characteristics of canines and the presence of MAP urolithiasis in canines, and to evaluate antimicrobial susceptibility patterns of bacteria isolated from canine uroliths. Methods: A total of 56 canines admitted for treatment with surgical removal of uroliths were recruited. Demographic characteristics and clinical chemistry data were recorded. Bacteria isolated from the removed uroliths were identified. Chemical compositions of the uroliths were analyzed by Fourier transform infrared spectrometer. Potential risk factors were determined with univariable and multivariable logistic regression analyses. Results: Of 56 canine urolithiasis, bacteria were isolated from uroliths of 38 canines (27 MAP and 11 non-MAP) but not from uroliths of 18 canines (5 MAP and 13 non-MAP). The most common bacteria found in nidus of MAP uroliths was Staphylococcus pseudintermedius (approximately 51%). An antimicrobial resistance was frequently found in Staphylococci isolates (42.86%). Multivariate logistic regression analysis showed that the predictors of MAP urolith in canine urolithiasis were being female (p = 0.044; adjusted odds ratio [OR], 10.22; 95% confidence interval [CI], 1.06-98.24) and the positive urolith culture (p = 0.012; adjusted OR, 8.60; 95% CI, 1.60-46.30). Conclusions: Our results indicate that S. pseudintermedius (a urease-producing bacterium) is the major causative bacteria of MAP uroliths. A positive urolith culture and being female are risk factors of MAP urolithiasis in canines.