• Journal of Microbiology and Biotechnology
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• v.31 no.7
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• pp.1035-1043
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• 2021

Although engineered Saccharomyces cerevisiae fermenting cellobiose is useful for the production of biofuels from cellulosic biomass, cellodextrin accumulation is one of the main problems reducing ethanol yield and productivity in cellobiose fermentation with S. cerevisiae expressing cellodextrin transporter (CDT) and intracellular β-glucosidase (GH1-1). In this study, we investigated the reason for the cellodextrin accumulation and how to alleviate its formation during cellobiose fermentation using engineered S. cerevisiae fermenting cellobiose. From the series of cellobiose fermentation using S. cerevisiae expressing only GH1-1 under several culture conditions, it was discovered that small amounts of GH1-1 were secreted and cellodextrin was generated through trans-glycosylation activity of the secreted GH1-1. As GH1-1 does not have a secretion signal peptide, non-conventional protein secretion might facilitate the secretion of GH1-1. In cellobiose fermentations with S. cerevisiae expressing only GH1-1, knockout of TLG2 gene involved in non-conventional protein secretion pathway significantly delayed cellodextrin formation by reducing the secretion of GH1-1 by more than 50%. However, in cellobiose fermentations with S. cerevisiae expressing both GH1-1 and CDT-1, TLG2 knockout did not show a significant effect on cellodextrin formation, although secretion of GH1-1 was reduced by more than 40%. These results suggest that the development of new intracellular β-glucosidase, not influenced by non-conventional protein secretion, is required for better cellobiose fermentation performances of engineered S. cerevisiae fermenting cellobiose.

• Journal of the Korean Society of Safety
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• v.39 no.1
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• pp.82-95
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• 2024

Analyzing the aftermath of events at domestic nuclear power plants brings in the question: "Why do workers not comply with the prescribed procedures?" The current investigation of nuclear power plant events identifies their reasons considering the factors affecting the workers' behaviors. However, there are some complications to it: in addition to confirming the action such as an error or a violation, there is a limit to identifying the intention of the actor. To overcome this limitation, the study analyzed and examined the reasons for non-compliance identified in nuclear power plant events by Reason's rule-related behavior classification. For behavior analysis, I selected unit behaviors for events that are related to human and organizational factors and occurred at domestic nuclear power plants since 2017, and then I applied the rule-related behavior classification introduced by Reason (2008). This allowed me to identify the intentions by classifying unit behaviors according to quality and compliance with the rules. I also identified the factors that influenced unit behaviors. The analysis showed that most often, non-compliance only pursued personal goals and was based on inadequate risk appraisal. On the other hand, the analysis identified cases where it was caused by such factors as poorly written procedures or human system interfaces. Therefore, the probability of non-compliance can be reduced if these factors are properly addressed. Unlike event investigation techniques that struggle to identify the reasons for employee behavior, this study provides a new interpretation of non-compliance in nuclear power plant events by examining workers' intentions based on the concept of rule-related behavior classification.

• The Journal of Korean Academic Society of Nursing Education
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• v.25 no.2
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• pp.206-215
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• 2019

Purpose: This study was conducted to examine the effects of an intervention program for human tissue donation with nurses, modified from the Korea Foundation for Human Tissue Donation, to promote human tissue donation via nurses' knowledge and attitudes toward human tissue donation, and self-efficacy for teaching tissue donation. Methods: A non-equivalent control group quasi-experimental design was adopted, and data were collected from 82 nurses (41 of each group: experimental and comparison) working at a general hospital in Inchon, South Korea. Results: In a multivariate analysis (MANOVA), the integrative effect on outcome variables from the intervention program was statistically significant (p<.001). Accordingly, an ANOVA was performed to determine which individual outcome variable showed a significant effect with intervention, and it was found that the effects of intervention on all the outcome variables (knowledge and attitude, and self-efficacy for teaching human tissue donation) were significant (p<.001). Conclusion: The results of the study showed that the intervention had positive effects on knowledge and attitudes toward tissue donation, and self-efficacy for teaching tissue donation among nurses. These outcome variables derived from the intervention might be essential for eliciting positive behavior toward human tissue donation.

• Korea Trade Review
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• v.47 no.5
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• pp.201-221
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• 2022

This study aims to analyze a harmonious approach between trade norms for the protection of human rights and the WTO agreements is increasingly necessary and important. conflicts and harmonization that may occur between major human rights protection trade norms and WTO agreements were comprehensively reviewed. The hard legalization of corporate social responsibility for sustainable development, such as human rights protection, was in conflict with the WTO Agreement, which was based on the principle of non-discrimination. As the currently expanding human rights protection trade norms reflect differences in the positions of developed and developing countries, it was also pointed out that there may be disputes over WTO compatibility and distorted protectionism measures. Accordingly, the applicability of the general exceptions to Article 20 of the GATT were reviewed together, and Article 20(a) of GATT, "necessary to protect public morals" may differ between developed and developing countries, and thus limitations were also considered. At the same time, When it is necessary to take regulatory measures such as prohibition of imports from a specific country for human rights protection, it was reviewed and proposed domestic trade norms revision.

• Human Ecology Research
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• v.60 no.4
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• pp.535-547
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• 2022

This study aimed to examine the effect of parental psychological control on adolescents' non-suicidal self-injury and verify whether this effect is mediated by depression. In the first survey of 647 middle and high school students, only those who had experiences of non-suicidal self-injury were selected (136 adolescents). For data analysis purposes, descriptive statistics for each measured variable and Pearson's correlation coefficients among research variables were calculated. In addition, Process Macro - Model 4 by Hayes (2013) was used to investigate the mediating model. Finally, the bootstrapping method was applied to confirm the significance of the mediating effect. The results of this study were as follows. First, parental psychological control and depression directly affected the frequency of non-suicidal self-injury among adolescents. Second, depression mediated the relationship between parental psychological control and non-suicidal self-injury. In conclusion, the more parents controlled their children's psychological factors, the higher the level of depression among adolescents, and as a result, the higher the frequency of non-suicidal self-injury. These findings suggest that educational intervention on parental psychological control is important to prevent and reduce non-suicidal self-injury; however, psychotherapy intervention that reduces the levels of depression should also be required.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.2
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• pp.599-606
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• 2012

The characterization of the whole genome of human papillomavirus type 16 (HPV16) from cervical cancer specimens with multiple infections in comparison with single infection samples as the oncogenic potential of the virus may differ. Cervical carcinoma specimens positive for HPV16 by PCR and INNO-LiPA were randomly selected for whole genome characterization. Two HPV16 single infection and six HPV16 multiple infection specimens were subjected to whole genome analysis by using conserved primers and subsequent sequencing. All HPV16 whole genomes from single infection samples clustered in the European (E) lineage while all multiple infection specimens belonged to the non-European lineage. The variations in nucleotide sequences in E6, E7, E2, L1 and Long control region (LCR) were evaluated. In the E6 region, amino acid changes at L83V were related to increased cancer progression. An amino acid variation N29S within the E7 oncoprotein significantly associated with severity of lesion was also discovered. In all three domains of the E2 gene non synonymous mutations were found. The L1 region showed various mutations which may be related to conformation changes of viral epitopes. Some transcription factor binding sites in the LCR region correlated to virulence were shown on GRE/1, TEF-1, YY14 and Oct-1. HPV16 European variant prone to single infection may harbor a major variation at L83V which significantly increases the risk for developing cervical carcinoma. HPV16 non-European variants prone to multiple infections may require many polymorphisms to enhance the risk of cervical cancer development.

• Journal of Radiation Protection and Research
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• v.41 no.1
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• pp.1-6
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• 2016

Background: It is very difficult to set a regulatory guidance or criteria for the protection of non-human species from the ionizing radiation, because there are no generally or internationally accepted methods for demonstrating the compliance with such criteria. It is needed that Korea develop the primary dose rate standards for the protection of both aquatic and terrestrial biota in the near future. Materials and Methods: The potential dose rates due to both external and internal radiation exposures to marine organisms such as plaice/flounder, gray mullet, and brown seaweed collected within territorial seas around the Korean Peninsula were estimated. Results and Discussion: The total dose rates to plaice/flounder, gray mullet and brown seaweed due to $^{40}K$, a primordial radionuclide in marine environment, were found to be 0.2%, 0.08% and 0.3% of approximately the values of the Derived Consideration Reference Levels (DCRLs, i.e. $1-10mGy{\cdot}d^{-1}$), respectively, as suggested by the International Commission on Radiological Protection (ICRP) publication 124. The total dose rates to marine fishes and brown seaweed due to anthropogenic radionuclides such as $^{90}Sr$, $^{137}Cs$ and $^{239+240}Pu$ were considered to be negligible compared to the total dose rate due to $^{40}K$. The external exposure to benthic fish due to all radionuclides was much higher than that of pelagic fish. Conclusion: From this study, it is recommended that the further study is required to develop a national regulatory guidance for the evaluation of doses to non-human species.

• Korean Journal of Community Nutrition
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• v.14 no.3
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• pp.277-285
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• 2009

This study was conducted to analyze the effect of male middle school students' eating school breakfast on their attitudes toward breakfast and school breakfast. In addition, the effect of school breakfast on breakfast-related eating behaviors and academic achievement was investigated. The study subjects were selected from a male middle school located in Gyeonggi-do, Republic of Korea. Breakfast was provided at the school for 7 weeks during the 2nd semester of the year 2006. Two sophomore classes were selected for the experimental study. All the students from one class (n = 34; School Breakfast Eaters) have eaten school breakfast while none of the students from the other class (n = 33; School Breakfast Non-eaters) have done so. About two weeks after the school breakfast service was terminated, questionnaires were distributed to the two classes and the responses were analyzed. The results showed that School Breakfast Eaters had more positive attitudes toward breakfast and school breakfast than School Breakfast Non-eaters. In addition, School Breakfast Eaters perceived the positive effect of eating breakfast on their school life more highly than School Breakfast Non-eaters. However there was no significant difference between the two groups in their breakfast eating behaviors in terms of breakfast skipping when the breakfast service was not available. No significant difference was found between the two groups with regards to math score variation before and after school breakfast service. In conclusion, school breakfast for 7 weeks had positive effects on male middle school students' attitudes toward breakfast and school breakfast, although the effect was not confirmed in their breakfast-related eating behaviors after the school breakfast service was terminated

• Journal of Microbiology and Biotechnology
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• v.25 no.3
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• pp.343-352
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• 2015

H9 is an ethanol extract prepared from nine traditional/medicinal herbs. This study was focused on the anticancer effect of H9 in non-small-cell lung cancer cells. The effects of H9 on cell viability, apoptosis, mitochondrial membrane potential (MMP; ${\Delta}\psi_{m}$), and apoptosisrelated protein expression were investigated in A549 human lung cancer cells. In this study, H9-induced apoptosis was confirmed by propidium iodide staining, expression levels of mRNA were determined by reverse transcriptase polymerase chain reaction, protein expression levels were checked by western blot analysis, and MMP (${\Delta}\psi_{m}$) was measured by JC-1 staining. Our results indicated that H9 decreased the viability of A549 cells and induced cell morphological changes in a dose-dependent manner. H9 also altered expression levels of molecules involved in the intrinsic signaling pathway. H9 inhibited Bcl-xL expression, whereas Bax expression was enhanced and cytochrome C was released. Furthermore, H9 treatment led to the activation of caspase-3/caspase-9 and proteolytic cleavage of poly(ADP-ribose) polymerase; the MMP was collapsed by H9. However, the expression levels of extrinsic pathway molecules such as Fas/FasL, TRAIL/TRAIL-R, DR5, and Fas-associated death receptor were downregulated by H9. These results indicated that H9 inhibited proliferation and induced apoptosis by activating intrinsic pathways but not extrinsic pathways in human lung cancer cells. Our results suggest that H9 can be used as an alternative remedy for human non-small-cell lung cancer.

• Nutrition Research and Practice
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• v.10 no.3
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• pp.359-363
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• 2016

BACKGROUND/OBJECTIVES: This study aimed to examine the association of Japanese schoolchildren's involvement in at-home meal preparation with food intake and cooking skill. SUBJECTS/METHODS: We included 1,207 fifth-grade children aged 10-11 years and one parent of each child. A cross-sectional survey was used to collect data on involvement in at-home meal preparation. Correspondence analysis was used to classify involvement in at-home meal preparation into three groups: food-related activities (cooking only or with other activities such as shopping, table-setting, clean up, and dishwashing), non-food-related activities (table-setting and/or clean up), and no (helping) activities. Food intake was assessed using a food frequency questionnaire. Logistic regression models were used to examine involvement in at-home meal preparation associations. RESULTS: The sample consisted of 1,207 fifth-grade children. Vegetable intake was lower in the no (helping) activities group than the food-related activities group (95% CI; boys: 1.2, 5.1, girls: 2.0, 8.9). Fewer children in the non-food-related activities group reported they were able to make a portion of their meals compared with the food-related activities group (95% CI; boys: 1.6, 3.5; girls: 1.5, 3.2). CONCLUSIONS: Children in the food-related activities group showed more favorable food intake and cooking skills than children in the no (helping) activities or non-food-related activities group.