• Title/Summary/Keyword: nodules

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Studies on Symbiotic Nitrogen Fixation in Soybean -I. Methods of Measurement and Amounts of Symbiotic N2 Fixation in Soybean Root Nodules (대두근류균(大豆根瘤菌)의 질소고정(窒素固定)에 관(關)한 연구 -I. 대두(大豆) 근류균(根瘤菌)의 질소고정력(窒素固定力) 측정방법(測定方法) 및 질소고정량(窒素固定量))

  • Ryu, Jin-Chang;Lee, Sang-Kyu;Lee, Hyuk-Ho;Hong, Chong-Woon;Cho, Moo-Je
    • Korean Journal of Soil Science and Fertilizer
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    • v.15 no.4
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    • pp.277-283
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    • 1982
  • A series of experiments were conducted on procedures of $N_2$ fixation measurements with soybean (Glycine max. L.) root nodules by acetylene reducing assay and estimation of amounts of $N_2$ fixed by soybean root nodules grown in the pot. The results are summarized as follows; 1. In case of nitrogen fixing activities were determined by means of acetylene reducing method, more appropriates results were obtained with direct determination of root nodules which was adhering from root under 2 hours after incubation, while more fluctuated data was obtained with root nodules determined under separated conditions. 2. The weight of soybean nodules was linearly increased from 17 days to 55 days after planting, whereas, the amounts of $N_2$ fixation from soybean nodules were Peaked at 38 days after planting, and declined thereafter. 3. When calculated upon the basis of the amounts of $N_2$ fixed as measured by acetylene reducing activities in single soybean plant, it was found that the average amount of symbiotic $N_2$ fixation falls in the range of 80 kg N per ha.

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Evaluation of the Usefulness of Differential Diagnosis of Thyroid Nodules using Elasticity Score and Strain Ratio in Elastogpraphy (탄성초음파에서 갑상샘 결절의 감별진단을 위한 탄성도 점수와 변형비의 유용성 평가)

  • Lee, Jin-Soo;Yang, Sung-Hee;Kim, changsoo;An, Hyun
    • Journal of the Korean Society of Radiology
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    • v.11 no.4
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    • pp.227-234
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    • 2017
  • This study evaluated the usefulness of the elasticity score and strain ratio in the differential diagnosis of benign and malignant nodules in thyroid elastography. We performed a retrospective analysis based on the results of fine needle aspiration cytology. The Chi-square test and the Mann-Whitney U test were used to analyze the difference between the five degrees of elasticity score and strain ratio according to the benign and malignant thyroid nodules. ROC curve analysis was used to determine the elasticity score and the best cut-off value of the strain ratio for the prediction of malignant nodules. There was a statistically significant difference (p=0.000) between the homogeneity of the elasticity score and the difference of the strain ratio between the benign and malignant nodule groups. On the ROC curve analysis, the elasticity score and the srain ratio for predicting benign and malignant nodules were determined as AUC 0.842, 0.700, cut-off value 3, 2.49 (p=0.001). Therefore, the elasticity score and strain ratio may be useful in the differential diagnosis of thyroid nodules.

Effects of root nodules on the plant type in soybean-Especially internode length and petiole length on the main stem

  • Ohashi, Shuma;Kurita, Haruna;Takahashi, Yukitsugu;Nagasuga, Kiyoshi;Nagaya, Yuichi;Umezaki, Teruhisa
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.358-358
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    • 2017
  • The plant type is generally one of the most important factor for crop production and be influenced by nitrogen absorption. Soybean plants have nodules in their roots, supplying nitrogen at the vegetative and reproductive stages. Root nodules seem to effect plant type of soybean plants, but there are few reports on the relation nodules and plant type. We tried to clarify the effects of root nodules on the plant type, especially internode length and petiole length, comparing non-nodule soybean with normal soybean. The pot experiment and field experiment were carried out at Mie University and Utsunomiya University in 2015 and 2016. Enrei, a popular cultivar in central Japan, and En1282, non-nodulating isogenic line of Enrei, were used. The petiole length on main stem was measured after defoliation and internode length and yield components were measured after harvest. In the field experiment, the patterns of the final length in internode and petiole on main stem were consistent in both cultivars, and a positive correlation was found between the Nth petiole length and the N-1th internode length, belong to the same phytomere. Therefore, the petiole and internode on the main stem make similar response for environmental factors. In pot experiment, Enrei grew with the same pattern as field experiments, but in En1282, the elongation of petiole and internode in the upper part was suppressed, especially the petiole was suppressed greatly. The main stem becomes the basis of the plant type. These results were considered that the nitrogen is distributed preferentially to the internode than the petiole. It seems that the pot cultivation restricted the rhizosphere and caused nitrogen deficiency in En1282. These results suggested that the slight nitrogen deficiency provided from the root nodules was compensated by the increase of the amount of inorganic nitrogen absorption due to the expansion of the rhizosphere, and the severe nitrogen deficiency suppressed elongation of petiole and internode. It is clear that root nodules effect the plant type by supplying nitrogen to internodes and petioles.

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Conventional Ultrasonography and Real Time Ultrasound Elastography in the Differential Diagnosis of Degenerating Cystic Thyroid Nodules Mimicking Malignancy and Papillary Thyroid Carcinomas

  • Wu, Hong-Xun;Zhang, Bing-Jie;Wang, Jun;Zhu, Bei-Lin;Zang, Ya-Ping;Cao, Yue-Long
    • Asian Pacific Journal of Cancer Prevention
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    • v.14 no.2
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    • pp.935-940
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    • 2013
  • Background: To evaluate the diagnostic utility of conventional ultrasonography and real time ultrasound elastography in differentiating degenerating cystic thyroid nodules mimicking malignancy from papillary thyroid carcinoma. Methods: We retrospectively analyzed conventional ultrasonographic and elastographic characteristics of 19 degenerating cystic thyroid nodules mimicking malignancy in 19 patients, with 30 surgically confirmed PTCs as controls. Based on size, the nodules had been grouped into less than 10mm (group A) and greater than 10 mm (group B). We evaluated conventional parameters and elasticity pattern. Color-scaled elastograms were graded as to stiffness of nodules using an elasticity pattern from I (soft) to IV (stiff). Results: Degenerating cystic thyroid nodules were similar to PTCs in conventional ultrasonographic findings, but the former frequently showed oval to round in shape (group A, 69.2% vs 18.8%, P=0.017; group B, 66.7% vs 7.14%, P=0.017) and punctuate hyperechoic foci (group A, 61.5% vs 0, P<0.001; group B, 50% vs 0, P<0.001). On real time ultrasound elastography, 7 of 13 degenerating cystic thyroid nodules in group A were pattern I, 5 were pattern II, 1 was pattern III. One degenerating cystic thyroid nodule in group B was pattern II, 5 were pattern III. The area under the curve for elastography was 0.98 in group A (sensitivity 92.3%, specificity 100%, P = 0.002), and 0.88 in group B (sensitivity 16.7%, specificity 100%, P = 0.014). Conclusions: As a dependable imaging technique, elastography helps increase the performance in differential diagnosis of degenerating cystic thyroid nodule and malignancy.

Effect of external-phosphorus supply on the phosphorus status of soybean nodules and the P-uptake system of isolated bacteroids (인산공급이 대두근류의 인산형태와 bacteroid의 인산흡수에 미치는 영향)

  • Sa, Tong-Min;Israel, Daniel W.
    • Applied Biological Chemistry
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    • v.34 no.2
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    • pp.117-124
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    • 1991
  • Soybean plants inoculated with Bradyrhizobium japonicum MN 110 were supplied with nutrient solutions containing 1.0, 0.25 and 0.5.nM-P to characterize the effect of externaI-P supply on the phosphorus status of nodules and on the P-uptake system of isolated bacteroids from nodules. After 48 days of growth, whole plant dry mass in the 0.25 and 0.05 mM-P treatments decreased significantly. The Pi concentrations in nodules were 4.1, 2.5 and 2.0 mM for 1.0, 0.25 and 0.05 mM-P treatments, respectively. The external-P supply did not significantly affect the distribution of phosphorus among inorganic phosphate(Pi), soluble organic-phosphorus(SOP) and insoluble organic-phosphorus(TOP) fractions in nodules. The Pi concentrations in young leaves of 0.25 and 0.05 mM-P plants were 33% and 20% , respectively, of those in young leaves of 1.0 mM-P plants and Pi concentrations in old leaves were only 16% and 7%, respectively, of those in old leaves of 1.0 mM-P plants. Phosphorus deficiency decreased the percentage of total leaf phosphorus in the Pi fraction and increased the percentage of total leaf phosphorus in the IOP fraction. The bacteroid number ranged from 0.87 to $1.30{\times}10^{11}$ Per GFW nodule regardless of external-P supply to the host Plants and Plant age, The P-uptake rates were the same (15-16 pmoles /min./$10^8$ bacteroids) for the bacteroids isolated from nodules of 1.0 mM-P and 0.05 mM-P plants. These results indicate that Pi concentrations in nodules of phosphorus-deficient plants are sufficient for proliferation of bacteroids and that the P-uptake system of bacteroids is in a repressed state even when host plant growth is severely restricted by phosphorus-deficiency stress.

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Effects of Two Different Rhizobium Strains on Nodulation and Growth of Lucerne (Medicago sativa L.) in an Acid Soil (균계를 달리하는 근류균이 산성토양 조건에서 알팔파의 근류형성과 생장에 미치는 효과)

  • Choe, Z.R.;Kim, J.K.;Bin, Y.H.
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.25 no.2
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    • pp.38-48
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    • 1980
  • To evaluate Rhizobium meliloti BALSAC, a strain selected from Canada as an acid tolerant one, and ordinary lucerne inoculant in acid condition, lucerne (Medicago sativa L. cv. Wairau) was inoculated and/or pelleted in the laboratory, and grown for two months in an acid soil (Lismore silt loam, pH 5.4) with three levels of lime in the, glasshouse. The results of controlled (noninoculated), nitrogen fertilized, laboratorial and commercial inoculated seeds were compared to give the following conclusions: 1. There was no significant difference in the top and root dry matter yields between two Rhizobium strains. However, Balsac inoculant showed higher single nodule dry matter weight and relatively higher number of larger nodules than the ordinary inoculant. 2. Lime application increased dry matter yields of plants and nodules, and the number of nodules per pot and the increase of nodules on the lateral roots in both inoculants. Lime application also caused an evenly distribution of nodules on the root by showing an increase of nodules mainly on the lateral roots. 3. Fertilizer nitrogen without inoculant slightly increased the nodulation percentage, the nodule dry matter weight per nodule and the relative proportion of larger nodules. 4. Commercially inoculated and pelleted seed showed less consistent results. 5. Relatively larger variations in measuring nodule characteristics was discussed and concluded that extreme cares should be given to reduce the variation.

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Expression of EuNOD-ARP1 Encoding Auxin-repressed Protein Homolog Is Upregulated by Auxin and Localized to the Fixation Zone in Root Nodules of Elaeagnus umbellata

  • Kim, Ho Bang;Lee, Hyoungseok;Oh, Chang Jae;Lee, Nam Houn;An, Chung Sun
    • Molecules and Cells
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    • v.23 no.1
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    • pp.115-121
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    • 2007
  • Root nodule formation is controlled by plant hormones such as auxin. Auxin-repressed protein (ARP) genes have been identified in various plant species but their functions are not clear. We have isolated a full-length cDNA clone (EuNOD-ARP1) showing high sequence homology to previously identified ARP genes from root nodules of Elaeagnus umbellata. Genomic Southern hybridization showed that there are at least four ARP-related genes in the genome of E. umbellata. The cDNA clone encodes a polypeptide of 120 amino acid residues with no signal peptide or organelle-targeting signals, indicating that it is a cytosolic protein. Its cytosolic location was confirmed using Arabidopsis protoplasts expressing a EuNOD-ARP1:smGFP fusion protein. Northern hybridization showed that EuNOD-ARP1 expression was higher in root nodules than in leaves or uninoculated roots. Unlike the ARP genes of strawberry and black locust, which are negatively regulated by exogenous auxin, EuNOD-ARP1 expression is induced by auxin in leaf tissue of E. umbellata. In situ hybridization revealed that EuNOD-ARP1 is mainly expressed in the fixation zone of root nodules.

X-ray Image Segmentation using Multi-task Learning

  • Park, Sejin;Jeong, Woojin;Moon, Young Shik
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.14 no.3
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    • pp.1104-1120
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    • 2020
  • The chest X-rays are a common way to diagnose lung cancer or pneumonia. In particular, the finding of a lung nodule is the most important problem in the early detection of lung cancer. Recently, a lot of automatic diagnosis algorithms have been studied to find the lung nodules missed by doctors. The algorithms are typically based on segmentation network like U-Net. However, the occurrence of false positives that similar to lung nodules present outside the lungs can severely degrade performance. In this study, we propose a multi-task learning method that simultaneously learns the lung region and nodule-labeled data based on the prior knowledge that lung nodules exist only in the lung. The proposed method significantly reduces false positives outside the lung and improves the recognition rate of lung nodules to 83.8 F1 score compared to 66.6 F1 score of single task learning with U-net model. The experimental results on the JSRT public dataset demonstrate the effectiveness of the proposed method compared with other baseline methods.

$^{99m}Tc-Red$ Blood Cell Scintigraphy of Sonographically Atypical Hemangioma (초음파상 비전형적 소견을 보이는 간혈관종의 적혈구 표지 스캔소견)

  • Lee, Kyoung-Soo;Lee, Ji-Young;Kim, Chan-Soo;Kim, Chang-Guhn;Choi, See-Sung;Won, Jong-Jin
    • The Korean Journal of Nuclear Medicine
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    • v.26 no.2
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    • pp.338-345
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    • 1992
  • We evaluated the scintigraphic findings of sonographically atypical hemangioma, that is, a hypoechoic or heterogeneously hypoechoic nodule which contains hypoechoic areas more than 40% of the volume, and compared it with those of sonographically typical hemangioma in 26 patients with 31 nodules. Sonographically atypical hemangioma were 13 in 11 patients and sonographically typical hemangioma were 18 the 15 patients. Dynamic blood flow, planar and SPECT blood pool imaging was performed. In atypical hemangioma, increased blood pool activity was seen in 11 of 13 nodules on SPECT and in 10 of 13 nodules on planar image and increased blood flow was seen in 4 of 13 nodules on dynamic blood flow study. No significant difference in detecting increased blood pool activity between sonographically atypical and typical hemangioma. In conclusion, $^{99m}Tc-red$ blood cell SPECT is as useful in diagnosis of sonographically atypical hemangioma as in typical hemangioma and can be used as a confirmatory or complementary study.

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The Efficiency of Voice Therapy for the Patients with Vocal Nodules (성대 결절 환자를 대상으로 한 음성치료의 효과)

  • 표화영;김명상;최홍식
    • Journal of the Korean Society of Laryngology, Phoniatrics and Logopedics
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    • v.8 no.2
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    • pp.178-184
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    • 1997
  • Vocal nodule due to vocal hyperfunction is one of the representative chronic diseases of vocal folds, and it can be cured by surgical movement, and/or voice therapy. The present study is, focusing on the latter, to compare the acoustic and aerodynamic results of the pretreatment with those of posttreatment, and then to investigate the objective date on the efficiency of the voice therapy for the patients with vocal nodules. 11 females(age : 7-49) and 5 males(age : 8-40), total 16 patients wi vocal nodules treated by voice therapy were participated as subjects. Six measurements and comparisons of pretreatment and posttreatment of the results were performed : litter, shimmer, and noise-to-harmonic ratio as acoustic analyses ; maximum phonation time, mean flow rate, and the subtraction of mean flow rate from maximum flow rate as aerodynamic analyses. As a result, 14 of 16 subjects showed improvement at more than 4 of 6 measurements, and in group data, every measurements of posttreatment was improved significantly than the pretreatment. On the whole, the improvement of aerodynamic aspects was more statistically significant than that of acoustic ones.

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