• Journal of Plant Biotechnology
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• v.3 no.3
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• pp.141-144
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• 2001

To enhance the shoot induction efficiency from nodal stem of yooza, the culture conditions such as basal medium, carbohydrate source, solidifying agent and the optimum concentration of plant growth regulators for shoot induction were investigated. The nodal explants were cultured better on MS medium than on MT, SH, B5 or W media in considering of shoot induction rate and mean shoot length. Solidifying agent in medium was better with 0.8% agar than with 0.3% agar, 1.2% agarose or 0.2% gelrite. Carbohydrate source in shoot induction medium was efficient with 30 g/L sucrose. The optimum concentrations of plant growth regulators were determined that 0.1 mg/L NAA as auxin was effective on the shoot induction, and 1.0 mg/L BAP as cytokinin induced multiple shoots efficiently. Shoot induction was the most effective on MS medium supplemented with 4 mg/L $GA_3$ in yooza.

• Plant Resources
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• v.1 no.1
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• pp.22-25
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• 1998

To establish direct multipropagation through organogenesis from nodal explants of Anoectochilus formosanus Hayata, the nodes were cultured on LS medium containing various concentrations of 6-benzyladenine(BA). High plant regeneration and adventitious bud formation were obtained from supplemented with 4.0mg/l of BA. Plant height was promoted by adding 0.3% activated charcoal. Plantlet regeneration capacity from nodes was depended on nodal parts on the stem, upper position was the best comparing with intermediate and lower.

• Korean Journal of Medicinal Crop Science
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• v.7 no.3
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• pp.155-161
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• 1999

This study was carried out to develop the propagation system using in vitro induced- microtubers of yams (Dioscorea alata L.). Effects of kinds of media, mineral composition, sucrose concentration (0, 2, 4, 6, 8, 10%), photoperiod (0, 8, 12, 16, 24h), and growth regulators (NAA, IAA, ZR, JA-Me, ABA) on the development of microtubers, roots, and shoots in nodal stem segment cultures of D. alata L. were evaluated. Microtuberization in nodal stem segment occurred on all the media supplemented with growth regulator and sucrose. Among basic media, 1/2MS medium was the best in microtuber induction. NAA was shown to be the most effective among the growth regulators. Optimal NAA concentration was 1mg/l. The microtuberization was the highest at the concentration of 6% sucrose. When the nodal stem segment were cultured under darkness, the tuberization was increased markedly compared to those cultured under light condition. It was also noticeable that the culture in medium with NAA produced only microtubers and roots, but no shoots, in nodal segments. In this study, the optimal medium composition for microtuberization in nodal stem segment was found to be 1/2MS medium supplemented with 1mg/l NAA and 6% sucrose under dark condition at $25^{\circ}C$.

• Journal of Bio-Environment Control
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• v.14 no.4
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• pp.298-301
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• 2005

Potato (Solamum tuberosum 'Dejima') plantlets were investigated on culture type and initial quantity of inoculation in bioreactor and survival rate by hydroponics for mass production. rode stems (1 to 1.5cm in length) of potato plantlets multiplied in vitro were grown for 3 weeks in liquid Murashige and Skoog (MS) medium with sucrose $30 g\; L^{-1}$. When plantlets (80-node inoculation) were raised in 10L balloon type bubble (BB) bioreactor, the healthiest growth of plantlets was obtained from explants cultured in ebb & flow culture with medium supplied periodically 12 times per day. The suitable inoculation quantity of 20L BB bioreactor was 120 pieces of stem segments (mean 2.2g fresh weight) in ebb & flow culture. Number of nodal shoot was eight on the average. In controlled culture room, survival rate of plantlets at 7 days after stem cutting was above $70\%$ when they were acclimatized by hydroponics grown in deep flow and solid medium culture. The highest survival rate of the stem cutting plantlets was in nutrient solution adjusted to EC $1.4dS{\cdot}m^{-1}$. Stem cutting plantlets through one culture could be obtained $670\~900$, when plantlets were grown in ebb & flow culture during 3 weeks using a 20L bioreactor with initial 120 pieces of nodal segments. 11 is possible In do mass production of seedlings cultured in bioreactor and hydroponics.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.1
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• pp.16-25
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• 1995

The effects of leaf removal time on variation of nodal sink components in determinated soybean ［Glycine max （L.） Merr.］ cultivar 'Danyeobkong' were measured at the experiment field of Chonbuk Provincial Rural Development Administration in 1991. Node order in this experiment was calculated from terminal node to bottom node to clarify the photosynthetic ability of canopy leaves. The upper 5 leaves from terminal node of main stem were removed at 5, 15, 25, 35 and 45 days after flowering(DAF) respectively. In spite of light-receiving increment in lower part of main stem and in branch by removing the upper part leaves of main stem, seed weight of that part was not increased in leaf removal treatment compared with control block. The node position reducing pod went down from terminal to bottom by delaying leaf removal time, and the influence of pod number reduction was bigger in DAF 25 than in DAF 15. But the seed number reduction per pod was the biggest in DAF 35. Cracked seed coat ratio ranged from 25% to 35% in control block, while that of leaf removal block had mostly 10% gap compared with the control block.rol block.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.10a
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• pp.16-16
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• 2019

The Cassava (Manihot esculenta Crantz) is a perennial woody shrub cultivated mainly in the tropics for its starchy tuberous roots. It belongs to the family Euphorbiaceae which also includes rubber (Hevea brasiliensis) and castor bean (Ricinus communis). Among tropical crops, rice, sugarcane, maize and cassava are the most important sources of calories for human consumption. Problems in the propagation of cassava are virus diseases and low rates of seed germination. Thus, a study was undertaken to develop an efficient in vitro mass propagation protocol of Manihot esculenta Crantz. Young and actively growing stem segments were excised from adult plants of cassava. Samples were cut into a 3~4 cm nodal segments with single node after sterilization, and cultivated in the different medium supplemented with various plant growth regulators for 4 weeks. For shoot multiplication, single-node stem segments, approximately 1 cm in length, were taken from in vitro derived shoots and subcultured. After 4~6 weeks, the shoot generation rate was 55.6%, the shoot number and its length were 1.0/explant and 2.3 cm in the most favorable medium composition. Our experiments confirmed that in vitro growth and multiplication of plantlets could depend on its reaction to the different medium composition, and this micropropagation techniques could be a useful system for healthy and vigorous plant production.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.3
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• pp.371-381
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• 1995

The effects of leaf and pod removal on variation of nodal sink components in determinate soybean [Glycine max (L.) Merr.] cultivar 'Danyeobkong' were measured at the experiment field of Chonbuk Provincial Rural Development Administration in 1991. Contrary to the conventional numbering system, node order in this experiment was numbered from top to bottom node. The leaves and pods of main stem from terminal to 5th node, below 6th node, or branches were removed at the growth stage of beginning pod(R3). In the leaf removal treatment, number and weight of pod and seed were highly decreased in upper part leaf removal, especially in removed part. In the pod removal treatment, number and weight of pod and seed were slightly increased in the other part. Cracked seed coat ratios were also high in the leaf removal treatment compared with control but low in the pod removal treatment.

• Korean Journal of Plant Tissue Culture
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• v.22 no.3
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• pp.131-135
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• 1995

Physiologically modified stem nodes derived from ex vitro and in vivo explants of hybrid aspen (Populus alba L.X P.grandidentata Michx. 'Crandon') were tested for their multiple shoot regeneration capacity using a broad spectrum dosage of cytokinins. Ex vitro derived stem nodes with excised axillary buds at the time of culture produced 11 to 13 multiple shoots on 20 to 30 $\mu$M zeatin containing Woody Plant Medium (WPM) after 6 weeks. Excision of axillary bud sprouts after 2 weeks of culture and culture of the remaining stem nodes on WPM with 1.0 to 2.0 $\mu$M BA or 10 to 30 $\mu$M zeatin produced 13 to 15 and 7 to 8 shoots per explant, respectively, Multiple tiny shoots were produced when in vivo derived stem nodes (on which all leaves were removed) were cultured on WPM with 30 to 50 $\mu$M 2iP or 20 to 50 $\mu$M zeatin. The greatest number of multiple tiny shoot proliferation (32 to 50 shoots per explant) were obtained when the explants were cultured on media containing 20 $\mu$M zeatin. Successful transplanting of these multiple shoots into the greenhouse and/or nursery was achieved.

• Journal of Korean Society of Forest Science
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• v.95 no.2
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• pp.155-159
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• 2006

An efficient method for in vitro propagation of the medicinal plant Hovenia duleis, was established. Plantlets for micropropagation of H. dulcis were obtained from in vitro germinated seeds. The effectiveness of various levels of cytokinins (BAP, Kinetin and TDZ) on multiple shoot formation from stem nodes was tested. BAP (1.0 mg/L) treatment induced highest number of multiple shoots. The growth pattern of plantlet on various culture media was undertaken. The shoot elongation was optimal on 2MS basal medium without growth regulators. The in vitro rooting ability of H. dulcis shoots was examined with two-auxins IAA and IBA. The IAA (1.0 mg/L) treatments induced earliest rooting with maximum number of roots and root growth. Rooted shoots were transferred directly to small pots with artificial soil and such established plant exhibited a normal growth pattern similar to wild plantlet.

• Horticultural Science & Technology
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• v.19 no.4
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• pp.483-487
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• 2001

This study was carried out to investigate the role of attached leaflet on rooting and growth by one nodal cutting in tomato. As cutting sources 4 to 6 nodes could be obtained from one nursery seedling. Medium for cutting was a mixture with perlite plus peat moss (1 : 1, v/v), and each plug capacity was 30mL in the 72 cells-plug tray. Plant height and the number of leaves were significantly increased by attached leaflet cutting in 'Pepe' and 'Coco' cherry tomato. Dry weights of top and root were increased as much as 3 to 15 times in the cutting attached leaflet. Rooting percentage was 93.5% in one node cutting and 86% in the cutting with hypocotyl node part. At 20 to 24 days after nodal cutting, healthy nursery plant could be produced to transplant in field. The nursery plants by nodal cutting (NPNC) showed more fibrous roots and less tap roots than that of seedling. In terms of rooting pattern, NPNC rooted at the whole ground stem part, while rooting of seedling occurred at basal part of hypocotyl.