• Asian-Australasian Journal of Animal Sciences
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• 제16권4호
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• pp.481-488
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• 2003

Insulin-like Growth Factors (IGFs) and IGF-binding protein act as intra-ovarian regulators that modulate the proliferation and differentiation of the granulosa and theca cells. Moreover, the IGF system is involved in metabolism by modulating the synthesis and degradation of glycogen and protein in animals. However the effect of the IGF system on egg productivity or body growth in KNOC has not been studied in depth. Therefore, this study was performed to investigate differences of serum IGFs and binding protein expressions between two groups showing high and low egg production or body weight and to elucidate the relationship of IGFs with egg productivity and body growth. KNOCs were divided into high and low groups depending on their egg productivity or body growth, and sera were collected every 10 wk from 20 till 60 wk. Serum IGF-I and -II concentration were measured by RIA using human and mouse antiserum and chicken standards. IGFBP was detected by Western ligand blotting. IGF-I concentrations were significantly greater in the high egg production group compared with those in the low egg production group (30 wk, p<0.01; 20 and 40 wk, p<0.05). Also, differences in IGF-II amounts between the two groups were detected at 60 wk (p<0.05). But IGFBPs in the low egg production group were more intense than that in the high egg production group through the egg laying period. The correlation between IGF-I concentration and number of egg production is significantly positive (20 wk, r=0.2729: p<0.05; 40 wk, r=0.3500: p<0.01), while IGF-II shows no correlation with egg productivity. In male KNOC, IGF-I and -II concentrations in the high body weight group are lower than that in the low body weight group. Body weight also shows a negative correlation with the serum IGF-II concentration in male chickens (20 wk, r=-0.5901: p<0.01). Consequently, we suggest that IGFs and binding protein are (in)directly involved in the egg productivity and body growth in KNOC.

• Asian-Australasian Journal of Animal Sciences
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• 제21권8호
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• pp.1134-1142
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• 2008

Cationic amino acid transporter $b^{0,+}AT$ (HGMW-approved gene symbol SLC7A9, solute carrier family 7, member 9) plays a crucial role in amino acid nutrition. In the present study, we describe the cloning and sequencing of porcine $b^{0,+}AT$. Based on the sequence of porcine $b^{0,+}AT$ deposited in the NCBI (National Center for Biotechnological Information), we identified a putative porcine homologue. Using rapid amplification of cDNA ends (RACE), the full-length cDNA encoding porcine $b^{0,+}AT$ was isolated. The porcine $b^{0,+}AT$ cDNA was 1,680 bp long, encoding a 487 amino acid trans-membrane protein. The predicted amino acid sequence was found to have 88.9% and 87.1% identity with human and mouse $b^{0,+}AT$, respectively. Real-time RT-PCR indicated porcine $b^{0,+}AT$ transcripts expressed in heart, kidney, muscle and small intestine. The small intestine had the highest $b^{0,+}AT$ mRNA abundance while the muscle had the lowest (p<0.05). Along the longitudinal axis, the ileum had the highest $b^{0,+}AT$ mRNA abundance while the colon had the lowest (p<0.05). The $b^{0,+}AT$ mRNA level was highest on day 7 and 90 in the duodenum (p<0.05). It increased from day 1 to day 26 in the jejunum (p>0.05) and had the highest abundance on day 60 (p<0.05). There was, however, no difference between day 1, 7, 26, 30, 90 and 150 (p>0.05). The strongest $b^{0,+}AT$ expression appeared on day 7 in the ileum before weaning, and then decreased till day 30 but rose gradually again from day 60 to 150 (p<0.05).

• Asian-Australasian Journal of Animal Sciences
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• 제29권6호
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• pp.814-822
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• 2016

This study was conducted to determine and compare the effects of yeast yeast culture (YC) and vitamin E (VE) supplementation on endotoxin absorption and antioxidant status in lactating dairy goats suffering from heat stress (HS). Three first lactation Saanen dairy goats (body weight $30{\pm}1.5kg$) were surgically fitted with indwelling catheters in the portal vein, mesenteric vein and carotid artery, and were randomly assigned to a $3{\times}3$ Latin square design. Dietary treatments were the basal diet, and the basal diet supplemented with either 100 IU VE or 30 g YC. Goats were kept in temperature and humidity-controlled room at $35^{\circ}C$ from 8:00 to 20:00 and at $24^{\circ}C$ from 20:00 till the next morning at 8:00. The relative humidity was kept at 55%. HS increased dairy goats' rectum temperature and respiration frequency (p<0.01). HS reduced plasma flux rate of milk goats (p<0.01), but the plasma flux rate increased when the animal was under the conditions of the thermo-neutral period (p<0.01). The VE supplementation lowered dairy goats' rectum temperature during thermo-neutral period (p<0.01). Meanwhile, no significant differences were observed between the control and YC treatment in rectum temperature and respiration frequency (p>0.05). Dietary supplementation of VE and YC reduced heat stressed dairy goats' endotoxin concentration of the carotid artery and portal vein (p<0.01). However, the endotoxin concentration of the YC treatment was higher than that of the VE treatment (p<0.01). Both VE and YC supplementation decreased heat stressed dairy goats' absorption of endotoxin in portal vein (p<0.01). The endotoxin absorption of YC treatment was higher than the VE treatment (p<0.01). The addition of VE and YC decreased dairy goats' superoxide dismutase (SOD) concentration during HS and the whole experiment period (p<0.01). The addition of VE lowered SOD concentration during thermo-neutral period (p<0.01). Likewise, the addition of VE and YC lowered dairy goats' malonaldehyde (MDA) concentration during HS and the whole experimental period, and the MDA concentration in the VE treatment was lower than the YC treatment (p<0.05). The addition of VE decreased MDA concentration during thermo-neutral period. On the contrast, the addition of VE increased dairy goats total antioxidant potential (TAP) concentration during HS, thermo-neutral and the whole experimental period (p<0.01). The addition of YC increased TAP concentration only during HS period (p<0.01). It is concluded that both VE and YC are useful in alleviating HS of dairy goats by weakening endotoxin absorption and promoting antioxidant capacity. Compared with YC, VE is much more powerful in easing dairy goats HS.

• 디지털융복합연구
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• 제12권1호
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• pp.475-489
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• 2014

외모도 능력의 일부라는 사회적 인식이 자리 잡게 되어 사회적 이미지를 유지해 가는 수단이 되어감에 따라 대부분의 사람들은 자기의 외모에 관심을 가지게 된다. 이에 여성들의 신체이미지와 자아이미지에 따른 화장태도를 알아보고자 대구광역시와 광주광역시에 거주하는 30세 이상 50세 미만 여성 218명을 대상으로 2012년 4월 10일 부터 5월 30일까지 2개월간 조사하여 분석한 결과는 다음과 같다. 첫째, 체중관심도와 자아이미지에 따른 화장태도의 차이를 알아본 결과 체중관심도의 경우 세련된 이미지와 순수한 이미지에 따라 화장태도에 차이를 보이지 않았지만 활동 이미지에서는 체중관심도에 따라 화장태도의 차이를 보였다. 둘째, 외모관심도와 자아이미지에 따른 화장태도의 차이를 알아본 결과 외모관심도가 높을수록, 세련된 이미지가 높은 집단, 순수한 이미지가 높은 집단, 활동이미지가 높은 집단이 화장태도에 더 적극적이었다. 즉, 외모관심도가 높을수록 그리고 자아이미지가 높을수록 화장태도에 대해 긍정적임을 알 수 있었다. 셋째, 신체매력도와 자아이미지에 따른 화장태도의 차이를 검증한 결과 신체매력도가 높을수록, 세련된 이미지가 높은 집단이, 활동 이미지가 높은 집단이 낮은 집단보다 화장태도가 긍정적이었다.

• 농촌지도와개발
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• 제13권1호
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• pp.149-171
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• 2006

This is a pilot study on rural women's psychological trap to define some obstacles to self directed learning. During few decades, according to major crop of each farm household has shifted from rice to other crops like as vegetables, fruits, horticultures, livestock, etc., women's role or labor sharing of women in farming has been also increased. Although women are important human resources, till now, there is no a research or an approach to rural woman on the view of individual human being. Therefore this study will contribute to understand woman's behavior or attitudes based on psychological description at each person's experiences. For this study, the data was collected from 23 women leaders who participated in a training course in 2005, through the scale of Jeffrey E. Young & Janet S. Klosko which was developed to improvement of one's repetitious behavior based on cognitive psychological care. It was categorized into 11types of psychological trap of one person, named as follows; (1) trap of being deserted by someone (2) trap of disbelief and being ill-treated (3) trap of weakness (4) trap of dependence (5) trap of emotional deprivation (6) trap of feelings of alienation among society (7) trap of deficiency (8) trap of anxiety to failure (9) trap of subordination (10) trap of the merciless standard by self-estimation (11) trap of the sense of privilege. From the data, the average age of subjects was 52.8years old, and the educational back of subjects was higher than general rural women. In both of the trap of weakness and the trap of the merciless standard by self-estimation, the ratio of over and 4 point score of 6 points was 71.4% and 76.2%. It means most of subjects have experienced fear of unexpected calamity(trap of weakness), and mental press hard for efforts to meet one's ideal standard(trap of the merciless standard by self-estimation). Especially the trap of the merciless standard by self-estimation may have relation with rural women's over burden from farming and local society activities.

• 건축역사연구
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• 제17권2호
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• pp.83-102
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• 2008

In the book of , it is recorded that the names of ingressive stone stairs to the main shrine were Cheongwoongyo(blue cloud bridge) and Baekwoongyo(white cloud bridge) and the names of stairs to the paradise hall were Yeonwhagyo(lotus bridge) and Chilbogyo(seven treasure bridge). But, the ingressive stone stairs to the main shrine and the paradise hall are single consecutive stairs with 2 levels respectively. Because of this, it is rather hard to clearly designate the names to the upper level and the lower level stairs. But, of the stairs to the Paradise hall, the lower stairs have a carving of lotus, and the upper stairs have seven stairs. In this aspect of artifacts, we can safely assume that the upper stairs are Chilbogyo and the lower are Yeonwhagyo. But, for the ingressive stairs to the main shrine, there is no such artificial hint. So, it is difficult to designate the upper and the lower stairs for the two bridges. Especially, it is not clear whether the descriptive order of "lower stairs upper stairs" in the names of Yeonwhagyo and Chilbogyo can be applied to the names of stairs to the main shrine. It is because the general descriptive order is "upper lower" rather than "lower upper." Even though there have been many studies on the Bulguksa temple, the study on the ingressive stone stairs to the main shrine has not made till now. Therefore, the study on the position of Cheongwoongyo and Baekwoongyo stairs can have its validity. In this paper, the positional approach to Cheongwoongyo and Baekwoongyo has been made in following aspects: First, the temple structure of Bulguksa; Second, the five element theory of oriental philosophy and the Book of Changes; Third, the directional consciousness of Buddhism. Through the aspects, the validity of up-down position of Cheongwoongyo and Baekwoongyo has been sought. In this research, it can be concluded that the upper level of ingressive stone stairs to the main shrine of Bulguksa is Cheongwoongyo and the lower level is Baekwoongyo. When considered in the Buddhist directional consciousness, it forms the total structure of "East-Cheongwoongyo South- Baekwoongyo West-Yeonwhagyo North-Chilbogyo."

• 생명과학회지
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• 제18권8호
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• pp.1042-1048
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• 2008

전립선 암은 현대 남성에게 걸리기 쉬운 질환으로 매년 점점 증가하는 암 사망률 중 하나이다. 하지만 남성호르몬 비의존형 전립선 암 치료에 대한 효과가 거의 없어 이에 본 연구에서는 남성호르몬 비의존형 전립선 암세포인 PC-3 세포에서 감마선이 세포 성장 억제와 세포자멸사 기작에 대해 알아보고자 한다. 그리하여 본 연구에서는 5가지 방법으로 즉, 세포증식 억제, apoptotic cell의 형태학적 변화, DNA 분절 분석, AV/PI 염색, western blot 분석법을 사용하여 수행하였다. 본 연구의 결과로 감마선을 처리한 PC-3세포에서 형태학적(분절화) 변화와 DNA ladder가 관찰되었다. 또한 감마선을 처리한PC-3세포에서는 apoptosis와 관련된 Caspase3와 PARP cleavage가 유발되었고 Bax 단백질 증가도 보였다.

• Clinical and Experimental Reproductive Medicine
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• 제30권1호
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• pp.95-103
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• 2003

Objective : To assess and compare the clinical outcomes between GnRH agonist long protocol and GnRH antagonist short protocol in oocyte donation program. Materials and Methods: Of total 18 oocyte donation cycles, controlled ovarian hyperstimulation (COH) were performed with GnRH agonist long protocol and GnRH antagonist short protocol in initial 9 cycles and later 9 cycles, respectively. Oral estradiol valerate and progesterone in oil we re administrated to all recipients for endometrial preparation. Oral estradiol administration was started from donor cycle day 1 after full shut down of gonadal axis with GnRH agonist in patients with ovarian function. Progesterone was injected from oocyte retrieval day of donor initially, then continuously till pregnancy 12 weeks if pregnancy was ongoing. We compared the parameters of clinical outcomes, such as number of the retrieved oocytes, fertilization rate, high grade embryo production rate, clinical pregnancy rate, implantation rate, ongoing pregnancy rate, COH duration, total gonadotropin dose for COH between GnRH agonist long protocol group and GnRH antagonist group. Statistical analysis was performed using Mann-Whitney test, p<0.05 was considered as statistically significant. Results: The number of retrieved oocytes, fertilization rate, high grade embryo production rate, clinical pregnancy rate, implantation rate, ongoing pregnancy rate were $14.89{\pm}7.83$, 81%, 64%, 78%, 31%, 78%, respectively in GnRHa long protocol group and $11.22{\pm}8.50$, 79%, 64%, 67%, 34%, 56%, respectively in GnRH antagonist group. There was no significant differences in parameters of clinical outcomes between 2 groups (all p value >0.05). Duration and total gonadotropin dose for COH were $10.94{\pm}1.70$ days and $43.78{\pm}6.8$ vials in 18 cycles, $12.00{\pm}1.73$ days and $48.00{\pm}6.93$ vials in agonist group, $9.88{\pm}0.78$ days and $39.55{\pm}3.13$ vials in antagonist group, respectively. In GnRH agonist long protocol group, significantly longer duration and higher gonadotropin dose for COH were needed (p=0.012). Conclusion: In oocyte donation program, clinical outcomes from controlled ovarian hyperstimulation with GnRH antagonist were comparable to those from GnRH agonist long protocol group, so controlled ovarian hyperstimulation with GnRH antagonist may be effective as GnRH agonist long protocol. At least there may not be harmful effects of GnRH antagonist on oocyte development and quality.

• 한국수소및신에너지학회논문집
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• 제17권4호
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• pp.379-388
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• 2006

Two mesophilic trickling bed bioreactors filled with two different types of media, hydrophilic- and hydrophobic-cubes, were designed and conducted for hydrogen production under the anaerobic fermentation of sucrose. Each bioreactor consisted of the column packed with polymeric cubes and inoculated with heat-treated sludge obtained from anaerobic digestion tank. A defined medium containing sucrose was fed by the different hydraulic retention time(HRT), and recycle rate. Hydrogen concentrations in gas-phase were constant, averaging 40% of biogas throughout the operation. Hydrogen production rate was increased till $10.5\;L{\cdot}h^{-1}{\cdot}L^{-1}$ of bioreactor when influent sucrose concentrations and recycle rates were varied. At the same time, the hydrogen production rate with hydrophobic media application was higher than its hydrophilic media application. No methane was detected when the reactor was under a normal operation. The major fermentation by-products in the liquid effluent of the both trickling biofilters were acetate, butyrate and lactate. In order to run in the long term operation of both reactor filled with hydrophilic and hydrophobic media, biofilm accumulation on hydrophilic media and biogas produced should be controlled through some process such as periodical backwashing or gas-purging. Four sample were collected from each reactor on the opposite hydrogen production rate, and their bacterial communities were compared by terminal restriction fragment length polymorphism (T-RFLP) analysis of PCR products generated using bacterial 16s rRNA gene primers (8f and 926r). It was expressed a marked difference in bacterial communities of both reactors. The trickling bed bioreactor with hydrophobic media demonstrates the feasibility of the process to produce hydrogen gas. A likely application of this reactor technology can be hydrogen gas recovery from pre-treatment of high carbohydrate-containing wastewaters.

• 동의생리병리학회지
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• 제27권1호
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• pp.26-33
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• 2013

The present study explored studies directed at elucidating the effectiveness of Korean medicine in the treatment of sterility and systematically analyzed the trends of studies on sterility. The standardization and objectification based on results for this study may contribute to the development of effective Korean medicine in reducing sterility. In the annual tendency of sterility-related clinical studies, the number of papers began to increase rapidly from 2000, and 15 papers were published from 2000 till 2011. Then, in the technical journals publishing them, The Journal of Oriental Obstetrics & Gynecology published 13 the most papers. In the institutions to which the first authors belonged, Dongguk University Hospital had 5 the most papers. In the number of test subjects was also analyzed and as the result the studies with 1~9 were the most as 10. In the study design analysis, there were 10 case reports, 5 case series and 4 before and after studies. In the analysis of intervention methods, there were 10 papers where only the Korean medicines were given, next 6 papers where the acupuncture and moxibustion were added, and 3 papers where the Korean medical therapies and other subsidiary therapies were used. In the analysis on the existence of pattern identification, there were 12 papers with the pattern identification, 6 without the pattern identification and 1 with the four constitution identification. In the IRB approval, 18 or most papers had not received the IRB approval and just 1 paper got the IRB approval. When the sterility-related clinical studies published in the Korean medical journals were surveyed, it turned out that there are no RCT studies and there is just one study with the IRB approval. And there were 6 papers without the pattern identification of Korean medicine. So, it seems necessary in the future to improve the papers in these respects for the development of Korean medicine sterility treatment with a good basis through the more systematic and accurate study method.