• 한국초전도ㆍ저온공학회논문지
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• 제19권2호
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• pp.29-32
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• 2017

The use of electronic devices such as mobile phones and tablet PCs has increased of late. However, the power which is supplied through wires has a limitation of the free use of devices and portability. Magnetic-resonance wireless power transfer (WPT) can achieve increased transfer distance and efficiency compared to the existing electromagnetic inductive coupling. A superconducting coil can be applied to increase the efficiency and distance of magnetic-resonance WPT. As superconducting coils have lower resistance than copper coils, they can increase the quality factor (Q-factor) and can overcome the limitations of magnetic-resonance WPT. In this study, copper coils were made from ordinary copper under the same condition as the superconducting coils for a comparison experiment. Superconducting coils use liquid nitrogen to keep the critical temperature. As there is a difference of medium between liquid nitrogen and air, liquid nitrogen was also used in the normal conductor coil to compare the experiment with under the same condition. It was confirmed that superconducting coils have a lower reflection-coefficient($S_{11}$) than the normal conductor coils.

• 한국수정란이식학회지
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• 제14권2호
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• pp.83-88
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• 1999

This study was undertaken with three objectives : to determine the optimal time of blood collection for plasma urea nitrogen(PUN) analysis, to examine the frequency distribution of PUN levels in recipient herd and to relate concentration of PUN to conception rate in Hanwoo recipients. The relationship between PUN level and time postfeeding was examined for 5 individual cows. Mean concentration of PUN rose for 4hrs postfeeding and decreased to PUN level before feeding at 10hrs postfeeding. And then, the blood for PUN analysis was collected at the time before feeding in next experiments. The ratio of cows with PUN concentration of < 12, 12∼18 and 18mg/dl were 50.6, 39.9 and 9.5% in 163 recipients, individually. The pregnancy rate of cows with PUN concentration 12∼16 mg/dl (63.3%) was higher than that of cows with PUN concnetration < 12 mg/dl (46.7%) or > 16mg/dl (42.9%). There results suggest that the PUN test may be beneficial for management of recipient herd in effects to maintain or improve reproductive efficiency.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2007년도 춘계학술대회B
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• pp.2194-2199
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• 2007

An experimental study is performed to investigate the thermal and flow characteristics of subcooled liquid nitrogen in a natural circulation loop. Experimental apparatus is designed and constructed such that a closed loop is cooled at the top by a cryocooler and heated nearly at the bottom by cartridge heaters. Steady state is obtained by controlling the heating power to the cartridge heaters and a thin-film heater to reduce the cooling power of the cryocooler. Temperature is measured at several locations of the loop and the mass flow rate through the loop is estimated from the energy balance in terms of the measured temperatures. Experiment is repeated for various values of the vertical height between the cooling and heating parts. The results show that the heat transfer capability of the loop has a maximum at a certain value of height. The optimal height to maximize the heat transfer is in a good agreement with analytical prediction to take into account the buoyancy and viscous forces in the loop.

• 한국수정란이식학회지
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• 제16권3호
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• pp.233-237
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• 2001

To develop an effective vitrification method, we examined the use of a conventional straw as vessel fur vitrification of mouse oocytes, and to compare the post-thaw survival and chromosome configuration of these oocytes with those vitrified in grids. Intact cumulus-enclosed oocytes were vitrified with DPBS with 5.5 M ethylene glycol and 1.0 M sucrose, and loaded into straws and onto eletron microscopic copper grid fur storing in liquid nitrogen. Intact vitrified and thawed oocytes were karyotying for chromosome. The rates of post-thawed survival were 88.5% in vitrified oocytes with straws, and 83% in vitrified ooctyes with grids. Vitrified and thawed oocytes with straws and grids were increased chromosomal abnormality (31.4% and 30.9%) compared with fresh oocytes (17.8%). The conventional straws can be used as vessel for vitrification to prevent of inflection in liquid nitrogen.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2002년도 생물공학의 동향 (X)
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• pp.269-270
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• 2002

Oxygen is a key substrate in animal cell metabolism and its consumption is thus a parameter of great interest for monitoring and control in animal cell culture bioreactor. The use of a gas-permeable membrane offered the possibility to provide the required quantity of oxygen into the culture. while avoiding problems of foaming or shear damage generally linked to sparging. For determining the optimum DO control strategy of this gas-permeable membrane aeration bioreactor, the oxygen transfer rate coefficient was measured with varying $N_2$ ratio in inlet air. The results showed that an increasing mass flow rate of nitrogen reduced the $K_La$ value. and 5% nitrogen in air did not result in any oxygen limitation.

• Journal of Microbiology
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• 제35권3호
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• pp.181-187
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• 1997

Filamentous cyanobacteria are an ecologically important group of bacteria because they are able to provide both organic carbon fixed nitrogen that can support the nutritional requirements for other microorganisms. Because of their prokaryotic nature, they can also be used as potentially powerful model systems for the analysis of oxygenic photosynthesis and nitrogen fixation. Gene transfer is an indispensable procedure for genetic analysis of filamentous cyanobacteria. Electroporation was used to introduce foreign DNA into cyanobacterial cells. In experiments designed to optimize the electroporation technique, the effects of the field strength (amplitude of pulse) and time constant (duration of pulse), DNA concentration and host restriction/modification of DNA on the efficiency of electro-transformation were investigated. The results of this research revelaed that a high voltage pulse of short duration was effective for the electro-transformation of Anabaene sp. M131. The maximal number of transformants was obtained at 6 kV/cm with a pulse duration of 5 msec. The efficiency of electro-transformation was also sensitive to concenetration of DNA; even small amounts of DNA (0.01 .mu.g/ml) were able to gie a large number of transformants (1.0 * 10$\^$3/ cfu/ml).

• 한국농업기계학회:학술대회논문집
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• 한국농업기계학회 1993년도 Proceedings of International Conference for Agricultural Machinery and Process Engineering
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• pp.443-452
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• 1993

Direct freezing tests of soybean seed by liquid nitrogen were carried out at various moisture contents and the following important conclusions were drawn from the results of temperature measurements of soybean seed and photographs of bubbles generated on its surface : 1) Assuming that the temperature gradient in a soybean seed is negligible because of its small seed size and the freezing ratio is followed the Heiss's formula, and a differential equation based on the heat energy balance was introduced . The equation was easily solved by the Runge-Kutta-Gill method and the predicted values of the temperature were in good agreement with the observed data. 2) The photographs of bubble generation during freezing showed the boiling mode was nucleate, and then the most suitable formula on the nucleate boiling heat transfer was introduced from many formulate proposed up to now by fitting the calculated values based on the formula to the observed data. The formula used for the predict on of the seed temperature was as follows: $\frac{{\partial}T_s}{\partial\theta}\;=\;-\frac{{\alpha}(T_s\;-\;T_L)^{3.3}}{W(C_s\;-\;\frac{{\delta}m(CT_s\;+\;{\sigma})}{T_s^2})}$ where C = difference of the specific heat between pure ice and water m=moisture content of soybean seed $T_s$ = seed temperature $T_L$ = Temperature of liquid nitrogen W = mass of soybean seed $\alpha$ = proportional constant $\delta$ = constant depends on variety or the type of seed $\theta$ = time $\sigma$ = latent heat of melting of pure ice This study will give important information in the hydro-freezing technique by liquid nitrogen, available as a new technique of processing agricultural products in the near future.

• Journal of Advanced Marine Engineering and Technology
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• 제32권5호
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• pp.724-729
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• 2008

Umbilical cord blood has been recently considered an attractive potential alternative as a source of stem cell transplantation to curing diseases such as leukemia, cancers, immune disorders. Normally the stored system of the umbilical cord blood specimen is equipped with a computer-controlled robotic arm that enables the samples to locate the identification places in liquid nitrogen tank at regulated temperature as about $-196^{\circ}C$. As the half of robotic arm is in the air and the rest part is submerged in liquid nitrogen, the temperature of robotic arm varies from ambient to liquid nitrogen temperature. In this study the temperature variation of upper part of arm above tank lid was thermally analysed by using the commercial code of Ansys. The result of analysis was that the upper part of robotic arm was seriously frozen due to heat transfer from liquid nitrogen as low as -$120^{\circ}C$. In order to solve the frost problem of robotic arm, small PTFE tube block as resistance material was introduced into the lower part of tank lid instead of the whole stainless steel(SUS) robotic arm. The results showed that the temperature of robotic arm above the lid was higher enough, and this method would be one of the very effective measure to solve the problem.

• 한국추진공학회지
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• 제15권2호
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• pp.68-73
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• 2011

소형위성발사체 (KSLV-I)의 발사 준비에는 다양한 규격의 질소 가스 및 초저온 액체 질소가 소요된다. 발사대 질소 공급 시스템은 고압의 질소 가스 생성, 추진제 충전 후 발사 대기 중인 발사체의 보호를 위한 공간 퍼지용 질소 가스 생성, 연료 및 산화제의 냉각을 위한 액체 질소 공급 기능을 수행하기 위하여 개발되었다. 대기 환경 조건에 따라 열전달 특성이 민감하게 변화하는 대기식 기화기의 운용 불안정성을 제거하기 위해 기화기의 병렬 설치 및 교대 운용 절차를 도입하였다. 이를 통하여 기후 조건에 상관없이 항시적으로 운용이 가능한 시스템을 개발하였으며, 소형 위성 발사체의 비행 시험을 위한 발사대 운용을 성공적으로 수행하였다.

• 한국추진공학회:학술대회논문집
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• 한국추진공학회 2010년도 제35회 추계학술대회논문집
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• pp.752-757
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• 2010

소형위성발사체 (KSLV-I)의 발사 준비에는 다양한 규격의 질소 가스 및 초저온 액체 질소가 소요된다. 발사대 질소 공급 시스템은 고압의 질소 가스 생성, 추진제 충전 후 발사 대기 중인 발사체의 보호를 위한 공간 퍼지용 질소 가스 생성, 연료 및 산화제의 냉각을 위한 액체 질소 공급 기능을 수행하기 위하여 개발되었다. 대기 환경 조건에 따라 열전달 특성이 민감하게 변화하는 대기식 기화기의 운용 불안정성을 제거하기 위해 기화기의 병렬 설치 및 교대 운용 절차를 도입하였다. 이를 통하여 기후조건에 상관없이 항시적으로 운용이 가능한 시스템을 개발하였으며, 소형 위성 발사체의 비행 시험을 위한 발사대 운용을 성공적으로 수행하였다.