• Food Science and Preservation
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• v.23 no.4
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• pp.591-598
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• 2016

The purpose of this study was to screen desirable yeast strains for alcoholic fermentation of tomato paste without sugar addition. The moisture, crude protein, crude fat, ash, and soluble nitrogen contents of the tomato paste ($25^{\circ}Brix$) were found to be 67.33%, 1.90%, 0.03%, 0.02%, and 30.72%, respectively. Free sugars found in the paste were fructose and glucose. Most abundant free amino acids of the paste were glutamic, aspartic, and ${\gamma}$-aminobutyric acids. Total seven yeast strains (Saccharomyces cerevisiae KDH (TWA), S. cerevisiae Lalvin ICVD-47 (TWB), S. cerevisiae Lalvin RC-212 (TWC), S. cerevisiae Lalvin K1-V1116 (TWD), S. bayanus Lalvin EC-1118 (TWE), S. cerevisiae Enoferm (TWF), and S. cerevisiae DJ97(KCTC8842P) (TWG)) were tasted for alcohol fermentation of the tomato paste. The highest alcohol content (8.2%) and the lowest residual sugar content ($13.25^{\circ}Brix$) were observed in the tomato paste fermented using the S. cerevisiae Lalvin ICVD-47 strain (TWB) after 3 day and 4 day of fermentation, respectively. Sugar and reducing sugar contents, and pH of the tomato paste were not remarkably affected by the difference in yeast strains used, showing $13.25{\sim}13.45^{\circ}Brix$, 28.37~28.48 mg/mL, and 4.43~4.54, respectively, after 4 day of fermentation. Color and total acid content were significantly affected by the types of yeast strains and fermentation time, but the numerical changes were negligible. These results indicate that TWB would be the suitable strain for alcoholic fermentatiom of tomato paste based on its highest alcohol production and the lowest residual sugar content produced during fermentation.

• Korean Journal of Plant Resources
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• v.24 no.5
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• pp.642-649
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• 2011

Ginseng growth is largely affected by characteristics of soil in Ginseng field. In this study, we determined the critical ranges of physico-chemical properties of soil for optimization of ginseng growth by analyzing the soils from Anseong and Pocheon regions in Gyeonggi province. Fresh weight of ginseng was 2 to 5 fold higher in good growth field compared to poor growth field within Anseong region. In the case of Pocheon region, 1.5 to 2 fold differences of fresh weight of ginseng was observed between good and poor growth field. These results indicate the difference of ginseng growth even in the same region. Based on these results, critical ranges of physico-chemical properties of soils were determined by comparing the good and poor growth field of each regions, which are follows; more than 50% of soil porosity, 2.0~2.8 g/kg of total nitrogen, 500~900 mg/kg for Av. $P_2O_5$, 2.3~3.5 $cmol_c\;kg^{-1}$ for Exch. Ca in Anseong; less than 13% of liquid phase, 400~650 mg/kg for Av. $P_2O_5$, 4.0~4.7 $cmol_c\;kg^{-1}$ for Exch. Ca, less than 0.8 and 0.5 $cmol_c\;kg^{-1}$ for Exch. Mg and K, respectively, in Pocheon. Interestingly, we found that ginseng growth was affected by exchangeable base ratio (Ca:Mg:K) especially in Anseong region, which were 6:2:1 in good growth field while 4:2:1 in poor growth field. Critical ranges for nutrient contents of ginseng leaves were also characterized, which are less than 0.2% and 0.22% of each P and Mg, respectively, in Anseong, while less than 1.8% and 0.18% of each N and P, respecively, and 1.5~3.0% of K in Pocheon. In addition, we determined critical ranges for inorganic nutrient contents in the current study.

• Applied Biological Chemistry
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• v.23 no.1
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• pp.64-72
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• 1980

Industrial wastes from pulp and food plants were treated with microorganisms to clarify organic waste-water and to produce cells as animal feed, and results were summarized as follows. (1) Waste-water from pulp, beer, bread yeast, and ethanol distillation plants contained $1.4{\sim}1.5%$ of total sugar, $0.25{\sim}0.35%$ nitrogen, and biological oxygen demand (BOD) was $400{\sim}25,000$, chemical oxygen demand (COD), $500{\sim}28,000$, and pH, $3.8{\sim}7.0$. The BOD and COD were highest in waste-water from ethanol distillation plants among others. (2) Bacterial and yeast counts were $4{\times}10^4-1{\times}10^9,\;2{\times}10^2-7{\times}10^4/ml$ in waste-water. (3) Bacteria grew better in pulp waste and yeasts in beer, bread yeast, and ethanol distillation waste. (4) Saccharomyces cerevisiae SAFM 1008 and Candida curvata SAFM 70 were the most suitable microorganisms for clarification of ethanol distillation waste. (5) When liquid and solid waste from ethanol distillation were treated with microbial cellulase, xylanase, and pectinase, solid waste was reduced by 36%, soluble waste was increased, and recuding sugar content was increased by 1.3 times which provided better medium than untreated waste for cultivation of yeasts. (6) Optimum growth conditions of the two species of yeast in ethanol distillation waste were pH 5.0, $30^{\circ}C$, and addition of 0.2% of urea, 0.1% of $KH_2PO_4$ and 0.02% of $MgSO_4$. (7) Minimum number of yeast for proper propagation was $1.8{\times}10^5/ml$. (8) C. curvata70 was better than cerevisae for the production of yeast cells from ethanol distillation waste treated with microbial enzymes. (9) S. cerevisiae produced 16 g of dried cell per 1,000ml of ethanol distillation waste and reduced BOD by 46%. C. curvata produced 17.6g of dried cell and reduced BOD by 52% at the same condition. (10) Yeast cells produced from the ethanol distillation waste contained 46-52% protein indicating suitability as a protein source for animal feed.

• Journal of Korean Society of Forest Science
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• v.102 no.3
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• pp.315-322
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• 2013

This study was carried out to establish a management program and soil restoration plan through analysis of soil properties and visitor questionnaires in Songrim in Hadong, Korea. Soil bulk density in Songrim was slightly higher in the closed-woodlands ($1.31g/cm^3$) than in the open-woodlands ($1.39g/cm^3$). Soil bulk density in the closed-woodlands was higher in walking trails ($1.74g/cm^3$) than in forest areas ($1.39g/cm^3$), while the rates of pore space were lower in walking trails (42.6%) than in forest areas (50.5%). The soil porosity were lower in the closed-woodlands (34.6%) than in the open-woodlands (42.6%). Soil strength in surface soil was slightly lower in the open-woodlands ($8.5kgf/cm^2$) than in the closed-woodlands ($10.5kgf/cm^2$). The content of organic matter, total nitrogen and exchangeable cations of the woodlands was low compared with the optimum nutrient content for tree growth in Korea forest soil. According to the survey, the objective of visiting in Songrim was to enjoy recreation and landscape views. To conserve pine forest ecosystems in Songrim, the respondents said that it needs to the implement of closed-woodland periods, the establishment of smoking free zone, and the prohibition of garbage throwing and alcohol including disciplinary rules and education. Also the respondents said that pine forest ecosystem in Songrim is relatively sound, but the woodlands require the intensive management to the ecosystem and the introduction of native understory vegetation, such as grasses under pine forest ecosystem. It is recommended to designate the rest-year forest for a proper period in all woodlands to restore the Songrim soil rather than the alternation application between the rest-year for three years or non-rest-year trails, and to open partially the walking trails across the woodlands after the period. In addition, the forest within the woodland is need to designate a long-term rest-year.

• Korean Chemical Engineering Research
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• v.46 no.3
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• pp.581-584
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• 2008

The catalyst carriers of the mesoporous layer compounds were prepared to carry out the partial oxidation of methane(POM) to hydrogen. The catalytic activities of POM to hydrogen were investigated over Ru(3)/SPK and Ru(3)/SPM catalyst in a fixed bed flow reactor under atmosphere. In addition, the catalysts and carriers were characterized by BET, TEM, TPR. The BET surface areas of the silica-pillared $H^+-kenyaite$(SPK) and the silica-pillared $H^+-magadite$(SPM) were $760m^2/g$ and $810m^2/g$, repectively, and the average pore sizes were 3.0 nm and 2.6 nm, repectively. The nitrogen adsorption isotherms were type IV with developed hysteresis. The TEM showed that the mesoporous layer compounds were formed well. The Ru(3)/SPK and the Ru(3)/SPM catalyst were obtained high hydrogen yields(90%, 87%), and were kept constant high hydrogen yields even about 60 hours at 973 K, $CH_4/O_2=2$, $1.25{\times}10^{-5}g-Cat.hr/ml$. The TPR peaks of Ru(3)/SPK and the Ru(3)/SPM catalyst showed the similar reducibilities around 453 K and 413 K. It could be suggested that SPK and SPM had the physicochemical properties as oxidation catalyst carries from these analysis data.

• Korean Journal of Ecology and Environment
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• v.46 no.4
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• pp.551-560
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• 2013

Environmental factors and epilithic diatom communities in the lower streams near the harbor region of South Korean peninsula were examined during no monsoon period in May 2013. The sampling of water and epilithic diatoms was conducted at both streams, 19 regulated streams (RS) that there are one or several dams constructed in the river system, and 19 un-regulated streams (US) that there are no dams within the river. A cluster analysis based on the number of species and abundance of epilithic diatoms through the stations, divided into three groups such as groups I (mainly US), II (mixed with US and RS) and III (mainly RS), respectively. Group I showed that water quality is good and high diversity of diatom, while Group II and III was water quality is relatively poor, but not differed in biomass of diatom from Group I. In addition, Group II that had high conductivity, nitrogen and phosphorus, was the lowest in diatom diversity among them. Dominant species were Nitzschia palea (17%) and Navicula seminuloides (11%) in Group I, Nitzschia inconspicua (19%) and Navicula perminuta (9%) in Group II, and Nitzschia inconspicua (15%) and Nitzschia palea (14%) in Group III, respectively. These taxa were widely distributed in brackish water, and not closely related with specific water quality, like eutrophic water. However, the groups II and III belonged to RS, had not only little biomass, but bad water quality such as high concentrations of nutrient and chlorophyll-a. Therefore, to determine the effect of dam construction on the lower water ecosystem, the planktonic algae, which can occur algal bloom in the estuary, also was considered to be a parallel investigation.

• Journal of the Korean Chemical Society
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• v.48 no.5
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• pp.473-482
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• 2004

The homobimetallic anion, $M^+({\eta}^5-MeCp)Mn(CO)_2Mn(CO)_5^-(M^+=Na^+,\;PPN^+)$was disrupted by CH2CHCH2Cl in THF at various temperatures ($20^{\circ}C~50^{\circ}C$) under the pseudo 1st order reaction conditions where excess of allyl chloride was employed under a nitrogen atmosphere. This homobimetallic anion seems to be involved in a concerted reaction mechanism in which a four-centered transition state is proposed. After undergoing the transition state, this reaction eventually leads to (MeCp)Mn$(CO)_3$ on addition of CO and $({\eta}^1-allyl)Mn(CO)_5$, respectively. However, in case of $Na^+$ analog, $Na^+$ may play a novel counter ion effect on the disruption reaction either by transferring one terminal CO from the $Mn(CO)_5$ moiety on to the $({\eta}^5-MeCp)Mn(CO)_2$of the corresponding homobimetallic complex, eventually resulting in $({\eta}^5-MeCp)Mn(CO)_3$ or through the interaction between $Na^+$ and the leaving group (Cl) of allyl chloride. This reaction is of overall second order with respect to homobimetallic complex with the activation parameters (${\Delta}H^{\neq}=17.15{\pm}0.17kcal/mol,\;{\Delta}S^{\neq}=-9.63{\pm}0.10$ e.u. for $Na^+$ analog; ${\Delta}H^{\neq}=22.13{\pm}0.21 kcal/mol,\;{\Delta}S^{\neq}=9.74{\pm}0.19$ e.u. for $PPN^+$ analog reaction).

• Korean Journal of Environmental Agriculture
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• v.33 no.2
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• pp.103-110
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• 2014

BACKGROUND: Azadirachta indica has been widely used as environment-friendly organic materials because of its insecticidal properties. This study was carried out to investigate the acute toxicity and the subacute toxicity of Azadirachta indica extract(AIE) in rats. METHODS AND RESULTS: For the oral acute toxicity test, Sprague-Dawley rats were gavaged with 2.0 g/Kg bw of AIE. The $LD_{50}$ value was greater than 2.0 g/Kg bw for both male and female rats. For the subacute toxicity study, rats were treated with AIE at doses of 0.5, 1.0, 2.0 mg/Kg bw once a day for 4 weeks(n=10 animals per each group). There were no significant changes in body weight, food intake and water consumption observed during the experimental duration. In addition, no difference of relative kidney weight was observed among all treated groups. Serum creatinine level in the AIE 2.0 g/Kg group increased significantly compared with that of control group in male rats, but serum blood urea nitrogen was significantly decreased in a dose-dependent manner (p<0.05). Significant increase of serum cholesterol levels were observed in all AIE groups, compared with the control group, in the female rats (p<0.05). However, histopathological examination of the kidney did not reveal any significant lesions in all groups. CONCLUSION: On the basis of results, it could be concluded that oral administration AIE didn't cause any toxic response in kidney, except the increased serum cholesterol.

• Korean Journal of Organic Agriculture
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• v.17 no.2
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• pp.237-251
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• 2009

This study was carried out to evaluate the effect of replacing antibiotics by organic acid mixture on growth performances and blood metabolites in growing pigs. Twenty-five crossbred pigs (Large White${\times}$Landrace) at the age of 79 days were fed five different diets by supplementing organic acid mixture and chlortetracycline. The experimental diets were consisted of diets without antibiotics supplementation (control), diets added 100mg/kg of chlortetracycline to control diet (T1), diets added 100mg/kg of chlortetracycline and 0.1% of $Acidomix^{(R)}$ (comprising formic acid 25%, sorbic acid 10%, fumaric acid 10%) to control diet (T2), diets added 0.1% of $Acidomix^{(R)}$ to control diet (T3), and diets added 0.3% of $Acidomix^{(R)}$ to control diet (T4). The changes in feed conversion ratio, average daily gain and blood metabolites were investigated. Twenty-five pigs were allotted to five treatments with five replications of each and the experiment was conducted on the basis of complete randomized design for 6 weeks. Average daily gain was significantly (p<0.05) different between T4 and control diets. All treatments including diets added $Acidomix^{(R)}$ and chlortetracycline were slightly higher than control diets. The feed intakes did not show a significant difference between the control and other treatments, and did not give change in feed intake by the addition of $Acidomix^{(R)}$. No differences on feed conversion ratio among treatments were observed but T4 and T3 treatments showed lower value than other treatments. The concentrations of blood urea nitrogen, total cholesterol and triglyceride were significantly (p<0.05) different among treatments, but their values were within the normal range representing that effects on blood profile by organic acid or antibiotics supplementation were not found. The results from this study indicated that adding chlortetracycline or organic acid mixtures to diets showed tendency to improve average daily gain and feed conversion ratio in growing pigs. These results showed that antibiotics could be replaced by organic acid mixture in growing pig diets, leading to stimulated growth and improved feed conversion ratio.

• Journal of Korean Tunnelling and Underground Space Association
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• v.22 no.5
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• pp.591-610
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• 2020

Concrete with activated carbon and titanium dioxide (TiO₂) has been used to reduce the particulate matter (PM) in underground structures (e.g., tunnels) due to the high performance of nitrogen oxides (NOx) abatement. Damage (e.g. crack, spalling, or detachment) can be caused by the environmental and ageing effects on the surface of the particulate matter reduction concrete, installed on the tunnel lining. Therefore, it is important to evaluate the existence of spalling on the concrete surface for maintaining performance of NOx reduction. In this study, a basic research was performed for feasibility of spalling evaluation using electrical resistivity characteristics. Given the test results, the electrical resistivity was decreased as the ratios of activated carbon (0~15%) and TiO₂ (0~25%) were increased for specimens. Under a dry condition, electrical resistivity of cement specimens, mixed with activated carbon and TiO₂, was decreased up to 2.3 times, compared with the normal cement specimen. In addition, under saturation conditions (degree of saturation: 85~98%), electrical resistivity of cement specimens with activated carbon, was decreased up to 3.5 times, compared with the normal cement specimen. Regardless of the condition (dry or saturated), the difference of electrical resistivity values shows the range of 2.3~2.8 times between the mixing specimen (with activated carbon (15%) and TiO₂ (25%)) and the normal cement specimen. This study can help to provide basic knowledge for spalling evaluation using the electrical resistivity on the surface of the particulate matter reduction concrete in tunnels.