• Title/Summary/Keyword: nitrogen ($N_2$)

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Studies on the Aging of Beef at Adding the Proteolytic Enzyme -VI. Effects of Papain Treatment on the Enzymatic Digestibility of Beef- (단백질(蛋白質) 분해효소(分解酵素) 첨가시(添加時) 우육(牛肉)의 숙성(熟成)에 관(關)한 연구(硏究) -VI. Papain처리(處理)가 우육(牛肉)의 소화율(消化率)에 미치는 영향(影響)-)

  • Youn, Jung-Eae
    • Korean Journal of Food Science and Technology
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    • v.10 no.4
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    • pp.394-397
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    • 1978
  • In vitro digestibility study using pepsin was conducted on round muscle previously treated with papain and the change in the amounts of liberated amino nitrogen was followed during the time course of digestion procedure. The obtained results were summarized as follows. 1. As compared with control, enzyme treatment groups had higher digestibility and curtailed digestion time. 2. All the enzyme treatment groups showed the highest digestibility between 6 and 10 hours in the course of digestion procedure. 3. The amounts of liberated amino nitrogen were increased similarly to digestibility and digestion time. 4. As compared with control, enzyme treatment groups showed considerably higher amounts of liberated amino nitrogen, the liberation rate being the highest between 2 and 8 hours of digestion time.

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Estimation of Mass Discrimination Factor for a Wide Range of m/z by Argon Artificial Isotope Mixtures and NF3 Gas

  • Min, Deullae;Lee, Jin Bok;Lee, Christopher;Lee, Dong Soo;Kim, Jin Seog
    • Bulletin of the Korean Chemical Society
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    • v.35 no.8
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    • pp.2403-2409
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    • 2014
  • Absolute isotope ratio is a critical constituent in determination of atomic weight. To measure the absolute isotope ratio using a mass spectrometer, mass discrimination factor, $f_{MD}$, is needed to convert measured isotope ratio to real isotope ratio of gas molecules. If the $f_{MD}$ could be predicted, absolute isotope ratio of a chemical species would be measureable in absence of its enriched isotope pure materials or isotope references. This work employed gravimetrically prepared isotope mixtures of argon (Ar) to obtain $f_{MD}$ at m/z of 40 in the magnetic sector type gas mass spectrometer (gas/MS). Besides, we compare the nitrogen isotope ratio of nitrogen trifluoride ($NF_3$) with that of nitrogen molecule ($N_2$) decomposed from the same $NF_3$ thermally in order to identify the difference of $f_{MD}$ values in extensive m/z region from 28 to 71. Our result shows that $f_{MD}$ at m/z 40 was $-0.044%{\pm}0.017%$ (k = 1) from measurement of Ar artificial isotope mixtures. The $f_{MD}$ difference in the range of m/z from 28 to 71 is observed $-0.12%{\pm}0.14%$ from $NF_3$ and $N_2$. From combination of this work and reported $f_{MD}$ values by another team, IRMM, if $f_{MD}$ of $-0.16%{\pm}0.14%$ is applied to isotope ratio measurement from $N_2$ to $SF_6$, we can determine absolute isotope ratio within relative uncertainty of 0.2 %.

Estimating urinary energy value of rat from the urinary nitrogen content (쥐의 뇨질소함량(尿窒素含量)으로부터 뇨(尿) Energy 가(價)의 산출법(算出法))

  • Han, In-K.
    • Applied Biological Chemistry
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    • v.7
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    • pp.29-33
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    • 1966
  • This experiment was conducted to study the relationship between the energy value and the nitrogen content in the rat urine. Thirteen rats for ad libitum feeding group and 16 rats for two-meal group were employed in this experiment. The experimental period consisted of 22 days with a preliminary period of 10 days. During the last eight days of the experiment the urine was collected quantitatively. The rats fed two-meal per day excreted significantly (p<0.005) more nitrogen and energy in the urine than those fed ad libitum. A linear relationship between the energy concentration and nitrogen content was found. The urinary energy value was increased as the urinary nitrogen content was increased. A prediction equation was derived to compute the energy value from the content of nitrogen as follows: Y=8.924X+0.182 $S_{y{\cdot}x}=0.788$ where Y=urinary energy(kcal/100 ml) X=urinary nitrogen(gm/100 ml) Since the standard deviation of estimate is in small magnitude (0.788 kcal) when it is compared with the amount of intake of gross energy, digestible energy or metabolizable energy, this equation can be used safely to estimate the energy value from nitrogen content. Consequently, considerable amound of time and labor for the actual determination of energy can be saved. The ratio of energy to nitrogen was found to be 9.4 for ad adlibitum group and 8.6 for two-meal group. No significant difference between two group in this respect was observed.

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Evaluation of N2O Emissions by Nutrient Source in Soybean and Pepper Fields (콩과 고추재배지에서 양분 공급원별 N2O 배출량 평가)

  • Kim, Gun-Yeob;Lee, Sun-Il;Lee, Jong-Sik;Jeong, Hyun-Cheol;Choi, Eun-Jung
    • Korean Journal of Environmental Biology
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    • v.36 no.4
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    • pp.680-686
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    • 2018
  • Nitrogen fertilizers, hairy vetch, and slow-release complex fertilizers were applied to the soil during the cultivation of crops. The impact of these factors on $N_2O$ emission was quantitatively assessed and the GHGs reduction effect comprehensively evaluated. Among the three factors, the significant factors affecting $N_2O$ emission were mineral nitrogen>soil moisture>temperature. Yield and fertilizer utilization efficiency were highest in the slow-release complex fertilizer treatment. There was no significant difference in $N_2O$ emissions between the slow-release complex fertilizer treatments and the NPK+hairy vetch treatments. Comprehensive results showed that slow-release complex fertilizers treatment has high yield and fertilizer utilization efficiency but low $N_2O$ emission.

The Effects Nitrogen percentage and Processing Time on the AISI 420 martensitic stainless steel during Plasma nitriding

  • Lee, In-Seop
    • Proceedings of the Korean Institute of Surface Engineering Conference
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    • 2015.11a
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    • pp.289-290
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    • 2015
  • In this experiment, nitriding treatment has been performed at $400^{\circ}C$ with various $N_2$ content and with changing processing time on AISI 420 martensitic stainless steel to investigate the expanded martensite layer (${\alpha}^{\prime}_N$ layer) formation behavior. Nitriding was implemented with changing $N_2$ content from 10% to 25% for 15 hrs and processing time was changed from 4hr to 15hr at 25% $N_2$ content. After treatment, the behavior of the ${\alpha}^{\prime}_N$ layer was investigated by optical microscopy, X-ray diffraction, and micro-hardness testing. Potentiodynamic polarization test was also used to evaluate the corrosion resistance of the samples. It was found that the surface hardness and ${\alpha}^{\prime}_N$ layer thickness increases with increasing $N_2$ percentage and processing time. Although their corrosion behaviors are worse than the bare sample.

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Effects of Nitrogen Fertilization Rate at Different Nursery Soils on Seedling Characters and Endosperm Consumption in Rice Seedling (상토 종류별 질소시비량이 벼 어린모 묘소질 및 배아양분 소모에 미치는 영향)

  • Kim, Sang-Su;Choi, Min-Gue;Lee, Seong-Yong;Yoo, Chul-Hyun;Cho, Soo-Yeon;Jun, Byung-Tae
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.41 no.5
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    • pp.514-520
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    • 1996
  • To clarify the proper nitrogen application level for rasing rice infant seedling under different nursery soil, Dongjinbyeo was raised at seedling box with different basal nitrogen level. The results are as follows. The higher the nitrogen level was, the lower emergence rate and the lower establishment rate. The establishment rate was less than 90%, when the nitrogen was more than 2g/box in hill soil and more than 1g/box in paddy soil. The more the nitrogen level was, the higher the seedling height in hill soil, but was higher in the order of N-2, 3, 1 and 0g /box. Leaf number wasn't significantly different between nusery soils and among nitrogen levels when seedling was raised more than 6 days. The endosperm survival rate was decreased as the increased nitrogen level, but wasn't different between the nursery soils. Amount of root was decreased as the increased nitrogen level in paddy soil, but was heavier in the order of N-1, 0, 2 and 3g /box in hill soil. Mat formation was better as the nitrogen level was decreased in all nursery soils. Considering the emergence rate, seedling charactors and mat formation, the proper nitrogen levels seemed to be 2g /box for hill soil and 1g /box for paddy soil.

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Change in Influent Concentration of Domestic Wastewater from Separated Sewer and Biological Nitrogen and Phosphorus Removal of a Full Scale Air-vent SBR (분류식 하수관거로의 전환시 유입하수의 성상 변화 및 선회와류식 SBR공법의 처리 특성)

  • Lee, Jang-Hee;Kang, Ho
    • Journal of Korean Society on Water Environment
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    • v.28 no.1
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    • pp.63-70
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    • 2012
  • This study was carried out to investigate change in influent concentration of domestic wastewater flowed from a newly constructed separate sewer system (SSS) and biological nutrients removal efficiency of a full scale Air-vent sequential batch reactor (SBR, $600m^3/d$). The average concentration of $BOD_5$, SS, T-N and T-P from SSS were 246.5 mg/L, 231.6 mg/L, 42.974 mg/L, 5.360 mg/L, respectively which corresponds to 2.2times, 1.2times, 1.8times and 2.1times higher than those from the conventional combined sewer system (CSS). The removal efficiency of $BOD_5$, SS, T-N, and T-P for the Air-vent SBR operated with influent from SSS averaged 99.1%, 99.0%, 91.2%, and 93.5%, respectively. Especially the respective nitrogen and phosphorus removal was 15% greater than that of the SBR operated with influent from CSS. Simultaneous nitrification and denitrification (SND) was observed in an aerobic reactor(II) as a result of DO concentration gradient developed along the depth by the Air-vent system. In order to achieve T-N removal greater than 90%, the C/N ratio should be over 6.0 and the difference between $BOD_5$ loading and nitrogen loading rate be over 100 kg/day (0.130 kg $T-N/m^3{\cdot}d$). Even with high influent T-P concentration of 5.360 mg/L from SSS (compared with 2.465 mg/L from CSS) T-P removal achieved 93.5% which was 15.5% higher than that of the SBR with influent from CSS. This is probably due to high influent $BOD_5$ concentration from SSS that could provide soluble carbon source to release phosphorus at anaerobic condition. In order to achieve T-P removal greater than 90%, the difference between $BOD_5$ loading and phosphorus loading rate should be over 100 kg /day (0.130 kg $T-N/m^3{\cdot}d$).

Effects of Aluminum Sulfate Addition on Six-Week-Old Broiler Performance and Nitrogen Contents in Litter at the Sixth Week (Aluminum Sulfate 처리가 6주령 육계생산 능력 및 깔짚 내의 질소 함량에 미치는 영향)

  • 최인학;남기홍
    • Korean Journal of Poultry Science
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    • v.29 no.4
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    • pp.265-270
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    • 2002
  • This study was conducted to determine the effect of aluminum sulfate[Al$_2$(S0$_4$)$_3$$.$14H$_2$O], commonly referred to as ALUM, addition to broiler litter on 3 and 6 week old broiler performance and the nitrogen content of the litter at 6 weeks of age. The two treatment groups were 134 identical diets with the same protein levels, but one group(T$_1$) had ALUM added as a top dressing to the litter at a rate of 200 g ALUM / kg of rice bran, while T$_2$ did not have ALUM added to the litter. Feed consumption for T$_1$ was higher in the 22 to 42 day-old and 0 to 42 day-old periods (P<0.05). Body weight in T$_1$ was also higher in the 0 to 42 day -old period (P<0.0l). There was no difference, however, in the feed : gain ratio between T$_1$ and T$_2$ During the first 5 weeks, T$_1$ had significantly less(P<0.05 or 0.01) ammonia emission from the litter than T$_2$ but at 6 weeks there was no difference in ammonia concentration between the two groups. At 6 weeks, T$_1$ had a lower litter pH than Ta (P<0.05) and total Kjeldahl nitrogen(TKN) was higher far T$_1$ than T$_2$ (P<0.05). However,71 did not show any difference from T$_2$ in the content of NH4-N and NO3-N. In summary, the addition of ALUM to broiler litter improved broiler performance at 6 weeks, while increasing nitrogen content from the litter used as the nitrogen fertilizer although ammonia emission was increased in T$_1$ at 6 weeks.

Reasonable Split Application Method of Nitrogen Fertilizer for Early Dry Seeding Culture of Rice in Honam Plain Area (호남평야지에서 벼 건답직파 조기파종재배에 알맞은 질소 분시방법)

  • Kim, Sang-Su;Choi, Weon-Young;Back, Nam-Hyun;Choi, Min-Gyu;Park, Hong-Kyu
    • Korean Journal of Soil Science and Fertilizer
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    • v.40 no.6
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    • pp.460-464
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    • 2007
  • This study was carried out to elucidate the proper split application method of nitrogen fertilizer for early dry seeding culture of rice in Honam plain area from 1997 to 1998 in Korea. Dongjinbyeo was selected as rice variety for this experiment. The rate of $160kg\;ha^{-1}$ of nitrogen was split as 40-30-30% of total nitrogen at three different application time combination ; T1) basal-5th leaf-panicle formation(PF) stage, T2) 3rd leaf-5th leaf-PF, and T3) 3rd leaf-7th leaf-PF. The content of $NH_4-N$ in soil at 5th leaf stage was higher in top dressing plots(T2, T3) compared with basal application(T1), at 7th leaf stage it was most in top dressed at 3rd leaf and 5th leaf stage, but there was no difference at heading stage. Amount of nitrogen uptake and nitrogen use efficiency was higher in the order of T3, T2 and T1(basal application). Spikelet number per unit area was more in the order of T3, T2 and T1, but rate of ripened grain and 1,000 grain weight were not significantly different among three nitrogen split application methods. Milled rice yields were higher in top dressed plots compared with basal nitrogen application plots. From the results of this experiment, reasonable nitrogen split application method for early dry seeding culture of rice could be 40-30-30% of total nitrogen at 3rd leaf, 7th leaf and panicle formation stage.

Chemical Properties of Dairy Slurry for Liquid Composting (液狀콤포스트化 處理에 있어서 乳牛糞尿의 化學的 特性)

  • 홍지형;최병민
    • Journal of Animal Environmental Science
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    • v.1 no.2
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    • pp.165-171
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    • 1995
  • Aerobic treatment of animal slurries represents an increasingly popular option for farmers in the management of animal wastes. This study was performed to find out the chemical characteristics of dairy slurry associated with liquid-solid separation. Total solids concentration varies widely depending on the slurry manure handling systems. Hydrogen ion exponent(pH), volatile solids(VS), ammonia nitrogen(NH$_3$-N), nitrate nitrogen (NO$_3$-N), and chemical oxygen demand(COD) essentially depends on the total solids content of animal liquid wastes. Total solids content of the dairy slurry ranges from 6.6 to 7.5% depending on the feed slurry and separator. Separated liquids from dairy slurry have been successfully downed for up to about 21, 900mg/$\ell$ of the COD value. It has also been found that separated slurry decreased from 37.8 to 26.0mg/$\ell$ of the NO$_3$-N concentration.

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