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The Effect of Dietary Protein Levels on Nitrogen Metabolism in Young Korean Women (한국여성의 단백질 섭취수준이 질소대사에 미치는 영향)

  • 구재옥
    • Journal of Nutrition and Health
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    • v.21 no.1
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    • pp.47-60
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    • 1988
  • This study was performed to investigate the effect of dietary levels on protein metabolism in eight healthy Korean adult females. The 20-day metabolic study consisted of 2 day adaptation period and three 6-day experimental. Three experimental diets were low protein (LP : protein 44g), high protein(HP : protein 85g) and high animal protein (HAP : protein-84g). The apparent absorption and balance on nitrogen were significantly higher in high protein than in low protein diet. Nitrogen, absorption rate was about 75% for low protein and about 85% for high protein intake. The mean values of nitrogen balance were -1.28% for low protein and 0.78% for high protein diet. All the subjects were in negative nitrogen balance at the low protein intake while they were in positive nitrogen balance at the high protein intake. The mean daily urinary nitrogen excretion increased with increased level of protein intake. Urea nitrogen was the largest part of the urinary nitrogen. The ratio of urea nitrogen to total urinary nitrogen increased significantly for 79 to 85% as protein intake was doubled.

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Nitrogen Harvest Index in Some Varieties of Mulberry, Morus spp.

  • Kumar, Jalaja S.;Chakraborty, Chumki;Sarkar, A.
    • International Journal of Industrial Entomology and Biomaterials
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    • v.5 no.1
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    • pp.131-134
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    • 2002
  • Mulberry being the only food of silkworm, Bombyx mori L., is of great economic importance to the silk industry, The success in cocoon production mainly depends on the supply of quality leaves in sufficient quantity. In mulberry, where the economic product is leaf, the uptake of nitrogen from soil is very heavy and high responses to application of nutrients have been reported. Nitrogen supports vegetative growth particularly the leaf biomass. Variation in nitrogen harvest index and other physiological and yield contributing traits were estimated in five mulberry genotypes. Considerable variation was observed for nitrogen harvest index, protein yield per plant and harvest index. The correlation studies indicated the protein yield per plant was significantly correlated with leaf yield, nitrogen content in leaf, nitrogen harvest index and harvest index. The broad sense heritability estimates revealed that harvest index showed highest heritability (88.07%) followed by nitrogen content (82.52%), protein yield (70.28%) and nitrogen harvest index (66.52%).

Patterns of Nitrogen Excretion in Growing Pigs

  • Lee, K.U.;Boyd, R.D.;Austic, R.E.;Ross, D.A.;Han, In K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.11 no.6
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    • pp.732-738
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    • 1998
  • Three crossbred gilts weighing $61{\pm}2kg$ ($mean{\pm}SD$) and three gilts weighing $52{\pm}3kg $ on the day before the first treatment began (d -1) were used for each of two experiments (Exp. 1 and Exp. 2), respectively. In Exp. 1, all pigs were fed the experimental diet (CP 19%) from d -7 to the end of study (d 21) to verify that nitrogen retention is constant during the 21 -d period. In Exp. 2, pigs were fed the control diet (CP 15.5 %) from d -7 to d 8 and then the low-lysine diet from d 9 to d 16 in order to determine how rapidly dietary changes in amino acid composition results in a new equilibrium for nitrogen metabolism. The amount of urine nitrogen loss was not different over 21 days (p > 0.10). Rates of nitrogen retention were not different among pigs (p > 0.10) nor over time (p > 0.10). Average nitrogen retention during the period was 1.00 g/kg $BW^{0.75}$ per day. The apparent biological value was 41%, which did not change over the 3-week period (p > 0.10). The overall efficiency of nitrogen use for nitrogen retention was 35% (Exp. 1). The amount of nitrogen loss in urine and the efficiency of nitrogen utilization for nitrogen gain reached a new equilibrium within 2 to 3 d after the diet was changed. The low-lysine diet resulted in a 20% increase of nitrogen loss in urine (p < 0.001) and a 9% decline in efficiency of nitrogen use for nitrogen retention (p < 0.001). Nitrogen retention while the pigs were fed the control diet was also higher than the retention when pigs were fed the low lysine diet (p < 0.001). The efficiency of nitrogen use for nitrogen retention in pigs fed the control diet was 57% (Exp. 2), which was higher (p < 0.001) than that from pigs fed the low-lysine diets (52%).

Demonstration of constant nitrogen and energy amounts in pig urine under acidic conditions at room temperature and determination of the minimum amount of hydrochloric acid required for nitrogen preservation in pig urine

  • Jongkeon Kim;Bokyung Hong;Myung Ja Lee;Beob Gyun Kim
    • Animal Bioscience
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    • v.36 no.3
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    • pp.492-497
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    • 2023
  • Objective: The objectives were to demonstrate that the nitrogen and energy in pig urine supplemented with hydrochloric acid (HCl) are not volatilized and to determine the minimum amount of HCl required for nitrogen preservation from pig urine. Methods: In Exp. 1, urine samples of 3.0 L each with 5 different nitrogen concentrations were divided into 2 groups: 1.5 L of urine added with i) 100 mL of distilled water or ii) 100 mL of 6 N HCl. The urine in open plastic containers was placed on a laboratory table at room temperature for 10 d. The weight, nitrogen concentration, and gross energy concentration of the urine samples were determined every 2 d. In Exp. 2, three urine samples with different nitrogen concentrations were added with different amounts of 6 N HCl to obtain varying pH values. All urine samples were placed on a laboratory table for 5 d followed by nitrogen analysis. Results: Nitrogen amounts in urine supplemented with distilled water decreased linearly with time, whereas those supplemented with 6 N HCl remained constant. Based on the linear broken-line analysis, nitrogen was not volatilized at a pH below 5.12 (standard error = 0.71 and p<0.01). In Exp. 3, an equation for determining the amount of 6 N HCl to preserve nitrogen in pig urine was developed: additional 6 N HCl (mL) to 100 mL of urine = 3.83×nitrogen in urine (g/100 mL)+0.71 with R2 = 0.96 and p<0.01. If 62.7 g/d of nitrogen is excreted, at least 240 mL of 6 N HCl should be added to the urine collection container. Conclusion: Nitrogen in pig urine is not volatilized at a pH below 5.12 at room temperature and the amount of 6 N HCl required for nitrogen preservation may be up to 240 mL per day for a 110-kg pig depending on urinary nitrogen excretion.

Use of Hairy Vetch Green Manure as Nitrogen Fertilizer for Corn Production

  • Seo, Jong-ho;Lee, Ho-jin;Hur, Il-bong;Kim, Si-ju;Kim, Chung-kuk;Jo, Hyeon-suk
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.45 no.5
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    • pp.294-299
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    • 2000
  • Hairy vetch (Vicia villosa Roth) winter annual is very effective on reducing chemical nitrogen fertilizer for subsequent com by fixed organic green manure nitrogen fixed during hairy vetch growth. In this experiment, hairy vetch produced above-ground dry matter of 5 ton/ha, nitrogen yield 200 kgN/ha, at com planting on the average during 1997 and 1998. Changes in com yield and nitrogen uptake for two years were investigated after application of nitrogen fertilizer 0, 60, 120, 180, 240 kgN/ha on plot of winter fallow and hairy vetch green manure, respectively. Nitrogen status such as ear-leaf N%, SPAD value at silk and dough stage, and com yield decreased in proportion to reduction of nitrogen fertilizer at winter fallow, but nitrogen status and yield of com were not different among nitrogen fertilizer rate at hairy vetch green manure. Com yield (total dry matter) at 0 kgN/ha plot of hairy vetch was 22, 20 ton/ha in 1997, 1998, respectively and com could produce more dry matter 9, 13 ton/ha by hairy vetch green manure compared with winter fallow under the condition of no nitrogen fertilizer in 1997, 1998, respectively. Com yield (total dry matter) at 60kgN/ha of hairy vetch green manure was higher than that of high N fertilizer rate such as 180, 240 kgN/ha of winter fallow. Nitrogen uptake of com at plot of hairy vetch-no nitrogen fertilizer slightly decreased than at plot of hairy vetch - nitrogen fertilizer, but com absorbed more nitrogen of 141, 159 kgN/ha by hairy vetch green manure compared with winter fallow under no nitrogen fertilizer condition in 1997, 1998, respectively. Nitrogen fertilizer reduction for com by hairy vetch green manure was 149, 161kgN/ha in 1997, 1998, respectively. Still more, com could absorb more soil nitrogen by nitrogen fertilizer 60kgN/ha of hairy vetch green manure than by high nitrogen fertilizer such as 180, 240 kgN/ha at winter fallow. It is concluded that nitrogen fertilizer for corn could be reduced by winter cultivation and soil incorporation of hairy vetch at com planting.

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Nitrogen Budget of South Korea Including Gaseous Nitrogen Oxides from 2012 to 2014 (기체상 질소산화물을 포함한 2012~2014년도 대한민국 질소수지 연구)

  • Lee, Hanuk;Oa, Seyeon;Park, Jae-Woo
    • Journal of Soil and Groundwater Environment
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    • v.22 no.4
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    • pp.49-59
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    • 2017
  • This study estimated the nitrogen budget, including gaseous nitrogen oxides ($NO_x$), of South Korea in 2012~2014. The nitrogen budget was classified into three categories: agricultural and livestock, forest, and city. To estimate the nitrogen budget, several input and output parameters were investigated, including deposition, fixation, irrigation, chemical fertilizer use, compost, fuel, denitrification, volatilization, runoff, crop uptake, leaching, and $NO_x$ emissions. The annual nitrogen inputs from 2012 to 2014 were 6,202,828, 6,137,708, and 6,022,379 ton/yr, respectively. The corresponding annual nitrogen outputs were 1,393,763, 1,380,406, and 1,360,819 ton/yr, respectively, signifying a slight decrease from 2012 to 2014. $NO_x$ was the parameter contributing to the nitrogen budget to the greatest extent. The annual ratios of $NO_x$ emissions by vehicles, power plants, and businesses were 0.31, 0.31, and 0.30 in 2012, 2013, and 2014, respectively. A change in government policy that prohibited the disposal of livestock manure and sewage sludge in the ocean from 2012 affected nitrogen budget profile. As a result, the ocean disposal ratio completely diminished, which differs from previous studies.

Changes of Chemical Compounds off the Compost of Municipal Refuse;2. Changes in Nitrogen Compounds (폐기물의 퇴비화 과정중 물질 변화;2. 질소화합물 변화)

  • Seo, Jeoung-Yoon
    • Korean Journal of Environmental Agriculture
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    • v.7 no.2
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    • pp.146-152
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    • 1988
  • In order to examine the seasonal variation of organic and inorganic nitrogen compounds, nitrogen compounds in compost were determined at various composting periods. Total nitrogen, organic nitrogen and biodegradable nitrogen contents in compost were almost not changed, while nonbiodegradable nitrogen contents were increased a little with the lapse of composting time. But effective contents of total nitrogen, organic nitrogen and biodegradable nitrogen were decreased with the progress of composting, while effective contents of nonbiodegradable nitrogen were not changed during composting. Ammonium nitrogen contents in compost were highest at the start of composting, and then the contents were decreased with the lapse of composting time. But after turning the contents were increased again, and thereafter the contents were decreased with the progress of composting. Nitrate contents showed a tendency adverse to ammonium nitrogen contents. Organic nitrogens in organic matter in compost were increased slowly within 9 weeks after composting, and thereafter the contents were increased rapidly to 21 weeks after composting. Total nitrogen contents determined by $F{\"{o}}rster$ Method were higher than those determined by Kjeldahl Method. Total nitrogen contents determined by Kjeldahl Method were 6% higher than biodegradable nitrogen contents determined by $F{\"{o}}rster$ Method. Loss of nitrogen in compost was highest at early periods of composting and its losses determined by Kjeldahl Method and $F{\"{o}}rster$ Method in 30 weeks after composting were 50% and 48% of total nitrogen, respectively. Highly positive correlations were observed among total nitrogen determined by Kjeldahl Method, biodegradable nitrogen determined by Kjeldahl Method, total nitrogen determined by $F{\"{o}}rster$ Method and biodegradable nitrogen determined by $F{\"{o}}rster$ Method one another.

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Nitrogen Utility during the Population Development with Different Clipping Treatments on Italian Rye Grass Field (Italian Rye Grass 초지군락의 청예처리빈도에 따른 생산성과 질소이용성)

  • 송승달
    • Journal of Plant Biology
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    • v.22 no.3
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    • pp.63-69
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    • 1979
  • The plant growth and net production, the nitrogen uptake and recycling, the nitrogen mobility and allocation to each organ, and the nitrogen utility from the Italian rye grass field during the population development were analyzed in comparison with different clipping treatments. The maximum dry matter standing crop and nitrogen quantity of harvest increased significantly, however, the annual amounts of dry matter and nitrogen assimilation showed little variations with increasing clipping frequencies. Plants treated with frequent clippings allocated relatively more nitrogen to leaves and less to roots during the experimental period. The amount of recycling of nitrogen decreased considerably due to frequent clippings. The annual averages of nitrogen utility indices changed in inverse relation to the nitrogen availability; such as 63, 58, 44 and 35 for C, A, M and J plots, respectively.

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Effect of High Nitrogen Application on Two Components of Dark Respiration in a Rice Cultivar Takanari

  • Akita, Shigemi;Lee, Kwang-hong
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.47 no.4
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    • pp.323-327
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    • 2002
  • Plant growth and the two components of respiration, growth and maintenance, were compared between low and high nitrogen applications in hydroponic culture on a high-yielding rice cultivar 'Takanari' (Oryza sativa L.). Grain yield decreased by high nitrogen application, and thus this cultivar has low adaptability to nitrogen. Growth efficiency (GE) and net assimilation rate (NAR) were lower in the high-nitrogen plot. The maintenance coefficient (m) and growth coefficient (g) of dark respiration were 0.0111 $d^{-1}$ and 0.196 in the low-nitrogen plot and 0.0166 $d^{-1}$ and 0.237 in the high-nitrogen plot, respectively. Thus, high nitrogen application increased both g and m. Calculated $R_m$ (maintenance respiration rate) was 70 and 90% of total respiration rate at heading, respectively. The significance of nitrogen adaptability and g was discussed.

Growth Simulation of Ilpumbyeo under Korean Environment Using ORYZA2000: II Growth Simulation by New Genetic Coefficients

  • Lee Chung-Kuen;Shin Jae-Hoon;Shin Jin-Chul;Kim Duk-Su;Choi Kyung-Jin
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2004.04a
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    • pp.102-103
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    • 2004
  • [ $\bigcirc$ ] In the growth simulation without changing of module with ORYZA2000, dry matter, LAI and leaf nitrogen content(FNLV) were estimated well under high nitrogen applicated condition, but overestimated under low nitrogen applicated condition. $\bigcirc$ Nitrogen stress factor on the SLA was introduced into ORYZA2000 because especially overestimated LAI under low nitrogen applicated condition was originated from SLA decrease with leaf nitrogen(FNLV) decrease. $\bigcirc$ In the growth simulation with modified SLA modified module, LAI was estimated well under even low nitrogen applicated condition, but dry matter was hardly changed compared with default. $\bigcirc$ Simulated plant nitrogen content and dry matter have no clear difference between modules, but compared with observed values, panicle weight(WSO) and rough rice yield(WRR14) were overestimated under high nitrogen applicated because of lodging, pest, disease and low nitrogen use efficiency.

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